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The map label for this gene is ykoW [H]

Identifier: 113968965

GI number: 113968965

Start: 715476

End: 717662

Strand: Direct

Name: ykoW [H]

Synonym: Shewmr4_0621

Alternate gene names: 113968965

Gene position: 715476-717662 (Clockwise)

Preceding gene: 113968963

Following gene: 113968966

Centisome position: 15.2

GC content: 50.71

Gene sequence:

>2187_bases
ATGTCAGATGGTTTAGCAAATCCACTCCAACAGGCATTAGTGACGATTTTTAGCGAAACGCCGCTAGAAACCCTAGAGCA
AAATGTTGACCAAGCCCTAGAGGCCATCACGCTGCAGTTGCATTGTGATGGGGTATTTGTGCTGACGGGCAGTTTAGCCC
TAGACCATCTACGTACCCGTAATCTGTATTTAAAACCGCAATTTAGCCAGGGCCAACAGACACGGGTCTGGCCGCTCGCG
CGTATGCCGTTTTTTCGCTCCTTAGTGAGAACCCCCACACTGCTGAATCTTCCCGATGTCGATACCTTGCCCGCGGAGGC
TCAAGCGGAGCGGGCGTTACTTCGTGATTGGAACGTTAAAAGCCTATTGGTGTTACCGCCTGTAGTGTTTGGCGAAACCC
GTATCGCCCTCGGCGCGGTCAATTGCTCGGAATGCTGTGAATGGAGTGCGGAGTTTATTCGGGAATTTCAACATGCGGCT
GTGATGATTGGCTCGGCGATGGAACTGACCCGTATCGCCCACGATATGTTGGCCAGTGAGCACAAATATCGCGAAGTTTT
TAATCAATTGCCTTTAGCCTGCGCCCTGCTCGATAAACAGAACCAACTGACCATGCTGAACAAAGTCGCGTTGCAAACCT
TGCCGGTGCAGCATGGATACGATTTATTCAGCATGGTGCGCGAAGAGGAGCATGCCATGCTCACCGACACGCTGCACATG
GTGCGCGAAGGCGTATTGGGGCAAGCCTGGTGTGAGTTACCCTTAAAATCTAACCTGCAGTTGGATTGGCTGAGGCTGAG
CTTTAGCCAAATTCACGGCAATAAAGATACCCTAGTGATGATTGCCGAGGATGTCAGTGAAAAATATCGCCTCGCCGATG
AGCTGTCCTTCCATGCCAATTATGATGCTTTGACTGGGTTACCGAATCGGCTGCATTTTGAGGCCTTGCTCGAAAATCTG
CTGCAAACCAAAGATGAGATGCCCACCTGCGTTGCCTTTATCGATGTCGATCAGTTTCAGGTGATCAACAATGTGAGCGG
CCATCAAGCCGGTGATAAATTACTGTGCCAGCTTGCATTACGTCTCAAGCAATTGGTTCGTAAGGGGGATATTGTCGCGC
GTTTGGGCGGCGATGAGTTTGGGATTTTAATGCATTATTGCAATGTGGATTCGGCGCAGCATATCGCTAATCGGATCTGC
ACTCAGTTAGCGACCCACGAGTTTATTTGGGAAAACCGTTGCCACAATGTCAGTGTCAGCATGGGGATTGCTAAGCTCGA
AAAGTCTGCGACGGATATTTACACTGTGATGAGTCAGGCCGATGCGGCCTGTCGTCTCGCGAAGGATAAGGGCCGCAATG
GCTGGCACTTGTACAGTGCCAAAGATCCTAAAATGACCCGCCTGTATACCGAGATGATGGCCTCAGTCGATATCGTCAGC
GCCCTCGCGCTAAATCAATTTGAGCTGTATTTCCAAAGCATTGCCCCGTTAAGTCGCGAAGAATCCGGCCTGCATTTAGA
GGTGCTACTGCGGATGGTGCAGGCCAATGGCACTATTGTTTCGCCGGCGATCTTTCTCCCCGCGGCGGAGCGTTACAACT
TGGCGGCCAAGGTCGACTTGTGGGTGATCGATAATCTGCTGAAGTGGGGCAGTAGCCATTTGGATATTTGGCAACAGCTG
GAGTTGGTCTCGGTCAACCTGTCGGCGACCTCGTTAGGTGACAGCGAGTTTATGAATTGGCTCGAAATGCGCCTGATGAC
CGAACCTGAGCTGGTGGATAAGCTCTGCATTGAGATCACCGAAACCGCCGCGGTGAGCCAATTAGATCAGGCCACTAAAT
TGATTGAAGTGCTGAGGCCATTAAACTGTCAACTCGCGTTGGATGATTTTGGCGCGGGGTTCTCAAGCTTTGCCTACCTT
AAACGCTTGAACGTGGATTATGTCAAAATCGACGGCCAGTTTGTGATTAATCTCTGCGAGGATGAGGCCGACCAAGCCAT
CATTAAAGCCATTTGCCAGTTAGGGCAAGATATGGGCTTCGATGTAGTGGCGGAGTTTGTTGAATCCACCGAAATTGCCA
CTAAGTTAAAAAGCCTAGGCGTGGATTATGCCCAAGGTTATGCCATCAACAAGCCTATGCCGTTACTGACTTTAACCTCT
GGCTTGGCCGAACCTTGGCTACGCTAA

Upstream 100 bases:

>100_bases
CTGCGCTAGTCCCATGATGGACTTCGACGACTTGAGTTTATCCGAGGTCAGCGTAGAAATAATACCTTAAATGGAATTGT
AGGTAAGGAAATCCCTTTTC

Downstream 100 bases:

>100_bases
CTTTAGGTTATCTTGCGCTGAGTTTTCGCAAATCTGCAATTCTTAACGGCTAAGCGACTCATTTACCACTATTTATTTAA
TTGTGATCCCTCGCCATTTG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MSDGLANPLQQALVTIFSETPLETLEQNVDQALEAITLQLHCDGVFVLTGSLALDHLRTRNLYLKPQFSQGQQTRVWPLA
RMPFFRSLVRTPTLLNLPDVDTLPAEAQAERALLRDWNVKSLLVLPPVVFGETRIALGAVNCSECCEWSAEFIREFQHAA
VMIGSAMELTRIAHDMLASEHKYREVFNQLPLACALLDKQNQLTMLNKVALQTLPVQHGYDLFSMVREEEHAMLTDTLHM
VREGVLGQAWCELPLKSNLQLDWLRLSFSQIHGNKDTLVMIAEDVSEKYRLADELSFHANYDALTGLPNRLHFEALLENL
LQTKDEMPTCVAFIDVDQFQVINNVSGHQAGDKLLCQLALRLKQLVRKGDIVARLGGDEFGILMHYCNVDSAQHIANRIC
TQLATHEFIWENRCHNVSVSMGIAKLEKSATDIYTVMSQADAACRLAKDKGRNGWHLYSAKDPKMTRLYTEMMASVDIVS
ALALNQFELYFQSIAPLSREESGLHLEVLLRMVQANGTIVSPAIFLPAAERYNLAAKVDLWVIDNLLKWGSSHLDIWQQL
ELVSVNLSATSLGDSEFMNWLEMRLMTEPELVDKLCIEITETAAVSQLDQATKLIEVLRPLNCQLALDDFGAGFSSFAYL
KRLNVDYVKIDGQFVINLCEDEADQAIIKAICQLGQDMGFDVVAEFVESTEIATKLKSLGVDYAQGYAINKPMPLLTLTS
GLAEPWLR

Sequences:

>Translated_728_residues
MSDGLANPLQQALVTIFSETPLETLEQNVDQALEAITLQLHCDGVFVLTGSLALDHLRTRNLYLKPQFSQGQQTRVWPLA
RMPFFRSLVRTPTLLNLPDVDTLPAEAQAERALLRDWNVKSLLVLPPVVFGETRIALGAVNCSECCEWSAEFIREFQHAA
VMIGSAMELTRIAHDMLASEHKYREVFNQLPLACALLDKQNQLTMLNKVALQTLPVQHGYDLFSMVREEEHAMLTDTLHM
VREGVLGQAWCELPLKSNLQLDWLRLSFSQIHGNKDTLVMIAEDVSEKYRLADELSFHANYDALTGLPNRLHFEALLENL
LQTKDEMPTCVAFIDVDQFQVINNVSGHQAGDKLLCQLALRLKQLVRKGDIVARLGGDEFGILMHYCNVDSAQHIANRIC
TQLATHEFIWENRCHNVSVSMGIAKLEKSATDIYTVMSQADAACRLAKDKGRNGWHLYSAKDPKMTRLYTEMMASVDIVS
ALALNQFELYFQSIAPLSREESGLHLEVLLRMVQANGTIVSPAIFLPAAERYNLAAKVDLWVIDNLLKWGSSHLDIWQQL
ELVSVNLSATSLGDSEFMNWLEMRLMTEPELVDKLCIEITETAAVSQLDQATKLIEVLRPLNCQLALDDFGAGFSSFAYL
KRLNVDYVKIDGQFVINLCEDEADQAIIKAICQLGQDMGFDVVAEFVESTEIATKLKSLGVDYAQGYAINKPMPLLTLTS
GLAEPWLR
>Mature_727_residues
SDGLANPLQQALVTIFSETPLETLEQNVDQALEAITLQLHCDGVFVLTGSLALDHLRTRNLYLKPQFSQGQQTRVWPLAR
MPFFRSLVRTPTLLNLPDVDTLPAEAQAERALLRDWNVKSLLVLPPVVFGETRIALGAVNCSECCEWSAEFIREFQHAAV
MIGSAMELTRIAHDMLASEHKYREVFNQLPLACALLDKQNQLTMLNKVALQTLPVQHGYDLFSMVREEEHAMLTDTLHMV
REGVLGQAWCELPLKSNLQLDWLRLSFSQIHGNKDTLVMIAEDVSEKYRLADELSFHANYDALTGLPNRLHFEALLENLL
QTKDEMPTCVAFIDVDQFQVINNVSGHQAGDKLLCQLALRLKQLVRKGDIVARLGGDEFGILMHYCNVDSAQHIANRICT
QLATHEFIWENRCHNVSVSMGIAKLEKSATDIYTVMSQADAACRLAKDKGRNGWHLYSAKDPKMTRLYTEMMASVDIVSA
LALNQFELYFQSIAPLSREESGLHLEVLLRMVQANGTIVSPAIFLPAAERYNLAAKVDLWVIDNLLKWGSSHLDIWQQLE
LVSVNLSATSLGDSEFMNWLEMRLMTEPELVDKLCIEITETAAVSQLDQATKLIEVLRPLNCQLALDDFGAGFSSFAYLK
RLNVDYVKIDGQFVINLCEDEADQAIIKAICQLGQDMGFDVVAEFVESTEIATKLKSLGVDYAQGYAINKPMPLLTLTSG
LAEPWLR

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=560, Percent_Identity=27.1428571428571, Blast_Score=189, Evalue=8e-49,
Organism=Escherichia coli, GI1788381, Length=453, Percent_Identity=31.5673289183223, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1788849, Length=361, Percent_Identity=29.9168975069252, Blast_Score=164, Evalue=3e-41,
Organism=Escherichia coli, GI87081921, Length=421, Percent_Identity=26.8408551068884, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI87082096, Length=235, Percent_Identity=35.7446808510638, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI1790496, Length=238, Percent_Identity=32.7731092436975, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI226510982, Length=429, Percent_Identity=26.5734265734266, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI87081980, Length=255, Percent_Identity=30.9803921568627, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789650, Length=448, Percent_Identity=23.8839285714286, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081881, Length=189, Percent_Identity=34.9206349206349, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081845, Length=241, Percent_Identity=27.8008298755187, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788502, Length=256, Percent_Identity=25.78125, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787055, Length=268, Percent_Identity=27.6119402985075, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=33.1428571428571, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1786507, Length=121, Percent_Identity=36.3636363636364, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=30, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=30, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081743, Length=214, Percent_Identity=28.0373831775701, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693134, Length=174, Percent_Identity=30.4597701149425, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081974, Length=122, Percent_Identity=39.344262295082, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787410, Length=153, Percent_Identity=30.0653594771242, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=104, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 81699; Mature: 81568

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGLANPLQQALVTIFSETPLETLEQNVDQALEAITLQLHCDGVFVLTGSLALDHLRTR
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECEEEEEECHHHHHHHHHC
NLYLKPQFSQGQQTRVWPLARMPFFRSLVRTPTLLNLPDVDTLPAEAQAERALLRDWNVK
CEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCC
SLLVLPPVVFGETRIALGAVNCSECCEWSAEFIREFQHAAVMIGSAMELTRIAHDMLASE
EEEECCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HKYREVFNQLPLACALLDKQNQLTMLNKVALQTLPVQHGYDLFSMVREEEHAMLTDTLHM
HHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VREGVLGQAWCELPLKSNLQLDWLRLSFSQIHGNKDTLVMIAEDVSEKYRLADELSFHAN
HHHCCCCHHHHCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCC
YDALTGLPNRLHFEALLENLLQTKDEMPTCVAFIDVDQFQVINNVSGHQAGDKLLCQLAL
CHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHH
RLKQLVRKGDIVARLGGDEFGILMHYCNVDSAQHIANRICTQLATHEFIWENRCHNVSVS
HHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHH
MGIAKLEKSATDIYTVMSQADAACRLAKDKGRNGWHLYSAKDPKMTRLYTEMMASVDIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ALALNQFELYFQSIAPLSREESGLHLEVLLRMVQANGTIVSPAIFLPAAERYNLAAKVDL
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEECCCEECCCHHHCCCEEEEHH
WVIDNLLKWGSSHLDIWQQLELVSVNLSATSLGDSEFMNWLEMRLMTEPELVDKLCIEIT
HHHHHHHHHCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ETAAVSQLDQATKLIEVLRPLNCQLALDDFGAGFSSFAYLKRLNVDYVKIDGQFVINLCE
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHHHCCCCEEEECCEEEEEECC
DEADQAIIKAICQLGQDMGFDVVAEFVESTEIATKLKSLGVDYAQGYAINKPMPLLTLTS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCEECCCCCCHHHHHH
GLAEPWLR
HHCCCCCC
>Mature Secondary Structure 
SDGLANPLQQALVTIFSETPLETLEQNVDQALEAITLQLHCDGVFVLTGSLALDHLRTR
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECEEEEEECHHHHHHHHHC
NLYLKPQFSQGQQTRVWPLARMPFFRSLVRTPTLLNLPDVDTLPAEAQAERALLRDWNVK
CEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCC
SLLVLPPVVFGETRIALGAVNCSECCEWSAEFIREFQHAAVMIGSAMELTRIAHDMLASE
EEEECCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HKYREVFNQLPLACALLDKQNQLTMLNKVALQTLPVQHGYDLFSMVREEEHAMLTDTLHM
HHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VREGVLGQAWCELPLKSNLQLDWLRLSFSQIHGNKDTLVMIAEDVSEKYRLADELSFHAN
HHHCCCCHHHHCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCC
YDALTGLPNRLHFEALLENLLQTKDEMPTCVAFIDVDQFQVINNVSGHQAGDKLLCQLAL
CHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHH
RLKQLVRKGDIVARLGGDEFGILMHYCNVDSAQHIANRICTQLATHEFIWENRCHNVSVS
HHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHH
MGIAKLEKSATDIYTVMSQADAACRLAKDKGRNGWHLYSAKDPKMTRLYTEMMASVDIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ALALNQFELYFQSIAPLSREESGLHLEVLLRMVQANGTIVSPAIFLPAAERYNLAAKVDL
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEECCCEECCCHHHCCCEEEEHH
WVIDNLLKWGSSHLDIWQQLELVSVNLSATSLGDSEFMNWLEMRLMTEPELVDKLCIEIT
HHHHHHHHHCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ETAAVSQLDQATKLIEVLRPLNCQLALDDFGAGFSSFAYLKRLNVDYVKIDGQFVINLCE
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHHHCCCCEEEECCEEEEEECC
DEADQAIIKAICQLGQDMGFDVVAEFVESTEIATKLKSLGVDYAQGYAINKPMPLLTLTS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCEECCCCCCHHHHHH
GLAEPWLR
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]