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The map label for this gene is ilvG [H]

Identifier: 113968706

GI number: 113968706

Start: 387107

End: 388780

Strand: Direct

Name: ilvG [H]

Synonym: Shewmr4_0362

Alternate gene names: 113968706

Gene position: 387107-388780 (Clockwise)

Preceding gene: 113968705

Following gene: 113968707

Centisome position: 8.23

GC content: 52.63

Gene sequence:

>1674_bases
ATGGAACCAGGGCAGATGATTAGAGGCGCAGACGCAGTCATAAAAGTGTTGGCAGCACATGGTGTTACCACAGTATTTGG
TTACCCAGGTGGTGCCATTATGCCAATCTACGATGCCCTCTATGGAAGCCCTGTCGAACATCTCTTAAGCCGCCACGAAC
AAGGTGCAGCCTTTGCCGCCGTTGGTTACGCCAGAGCCAGTGGTAAAACCGGCGTATGCTTTGCCACCTCAGGCCCTGGT
GCCACCAACTTAATCACTTCGCTTGCCGATGCGTTATTAGATTCTGTGCCTGTTGTCGCTATCACGGGTCAGGTTTCAAC
CGCTGTGATTGGCACTGATGCGTTTCAAGAAATCGATGTATTGGGCATGTCCTTAAGCTGCACTAAGCACAGCTTTATGG
TGACGGATGTAAACGATCTTATCCCGACCTTGTATCAAGCCTTCGAAATCGCGGCATCGGGTCGCCCTGGCCCAGTGCTG
GTGGATATCCCTAAAGATATCCAAATCGCGCAACTGGAATATCGCACTCCCTTGCTGGCGGTAACCAATGAACCACAGGC
GAGCGTCAGCGAGATTGACGCGGCCCGCGCCCTGTTAGCAGAGGCGAAGCAGCCTATGTTGTACGTGGGTGGTGGCGTCG
GGATGGCGGGCGCGGTCGATCAACTGCGGGAATTTATCAAAGCCACGGGTATGCCATCGGTTGCGACGTTAAAGGGCTTA
GGCAGTATTGCCCATGGCACTCCAGGTTATTTAGGCATGTTAGGTATGCATGGCGGAAAGGCGGCCAATCTAGCGGTGCA
GGATTGTGATTTATTAGTGGTTGCCGGTGCGCGCTTTGATGACAGGGTCACAGGGCGTTTAGCGACTTTTGCCAACAAGG
CTAAAGTCATCCATTTAGATATAGACGCCGCCGAGTTAGGCAAATTGCGCCAGCCCGATGTGGCCATTGCCGGTGATTTA
CGCCAGATTTTCCCTGCTTTAGCTATGGCGCTGAACATCACGCCTTGGCAAGCAGAGGTTGAACATCTTGCCCGTAAACA
TCAATGGGATTATCAACATCCCGGCAGCTTAATCTACGCTCCTGCCATGCTGCGCCGCCTTGCTAATAAACTGCCCGAAG
ACAGCGTAGTGAGCTGCGATGTGGGCCAACATCAAATGTGGGTCGCCCAGCATATGTGGTTCCGTCGCCCTGAAGATCAT
TTATCCAGCGCAGGTTTAGGCACTATGGGCTTTGGTTTACCCGCCGCTATCGGCGCCCAAGTCGCCCGCCCCGATGCAAC
TGTAGTCACAGTGTCTGGTGATGGCTCTTTTATGATGAACGTGCAGGAGCTGACCACCATCAAGCGCCGCAAATTACCGG
TGAAGATCCTACTAATCGACAACCAAAAATTGGGGATGGTGAAACAGTGGCAACAGCTATTCTTTGAAGAGCGCTACAGC
GAAACCGATCTGTCAGACAACCCCGATTTTGTGCAACTCGCTTCGGCCTTCGATATTCCCGGCCGCACGATTTTCTCTTC
CGACGAAGTGGAAGAGGCCTTAACCGAAATGTTAGCGGCTAAGGGTCCCTATTTGTTACACGTAGCAATCGACGATGCTT
TTAACGTTTGGCCACTGGTGCCCCCCGGCGCATCAAACAGCGATATGATGGACGAAATGGAGAAACAAACATGA

Upstream 100 bases:

>100_bases
TCTTCGTTTTCAGGCCCTAGGTTTTGGAAATGAGGTTTCGAAATAGCACAAATTACGAGTCGATTTTCGGCAATTCAGGA
TCAAGGTAGCGAGAGAGAAT

Downstream 100 bases:

>100_bases
TCCACTCCCTAGAATTAACGGTTCAACAACGCCCAGAAGTGATAGAACGTGTACTACGTGTGACTCGTCACCGTGGTTTT
ACTGTCACCCAAATGCAAAT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPVVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVTNEPQASVSEIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKATGMPSVATLKGL
GSIAHGTPGYLGMLGMHGGKAANLAVQDCDLLVVAGARFDDRVTGRLATFANKAKVIHLDIDAAELGKLRQPDVAIAGDL
RQIFPALAMALNITPWQAEVEHLARKHQWDYQHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVQLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT

Sequences:

>Translated_557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPVVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVTNEPQASVSEIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKATGMPSVATLKGL
GSIAHGTPGYLGMLGMHGGKAANLAVQDCDLLVVAGARFDDRVTGRLATFANKAKVIHLDIDAAELGKLRQPDVAIAGDL
RQIFPALAMALNITPWQAEVEHLARKHQWDYQHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVQLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT
>Mature_557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPVVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVTNEPQASVSEIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKATGMPSVATLKGL
GSIAHGTPGYLGMLGMHGGKAANLAVQDCDLLVVAGARFDDRVTGRLATFANKAKVIHLDIDAAELGKLRQPDVAIAGDL
RQIFPALAMALNITPWQAEVEHLARKHQWDYQHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVQLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=518, Percent_Identity=28.5714285714286, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=25.3141831238779, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=44.6460980036298, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=41.6520210896309, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=30.2491103202847, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=29.182156133829, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=26.0397830018083, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=525, Percent_Identity=28.7619047619048, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=26.4285714285714, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=26.4285714285714, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=42.3728813559322, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.2426470588235, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=476, Percent_Identity=24.7899159663866, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=470, Percent_Identity=24.0425531914894, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=481, Percent_Identity=22.8690228690229, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=502, Percent_Identity=28.0876494023904, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59613; Mature: 59613

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAA
CCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEE
VGYARASGKTGVCFATSGPGATNLITSLADALLDSVPVVAITGQVSTAVIGTDAFQEIDV
EEHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
LGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVLVDIPKDIQIAQLEYRTPLLA
HCCEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEEE
VTNEPQASVSEIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKATGMPSVATLKGL
ECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHC
GSIAHGTPGYLGMLGMHGGKAANLAVQDCDLLVVAGARFDDRVTGRLATFANKAKVIHLD
CHHCCCCCCHHEEEECCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEE
IDAAELGKLRQPDVAIAGDLRQIFPALAMALNITPWQAEVEHLARKHQWDYQHPGSLIYA
CCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEH
PAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMWFRRPEDHLSSAGLGTMGFGLPAAIGAQ
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHCCH
VARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQKLGMVKQWQQLFFEERYS
HCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
ETDLSDNPDFVQLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLV
CCCCCCCCCCEEHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PPGASNSDMMDEMEKQT
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAA
CCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEE
VGYARASGKTGVCFATSGPGATNLITSLADALLDSVPVVAITGQVSTAVIGTDAFQEIDV
EEHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
LGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVLVDIPKDIQIAQLEYRTPLLA
HCCEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEEE
VTNEPQASVSEIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKATGMPSVATLKGL
ECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHC
GSIAHGTPGYLGMLGMHGGKAANLAVQDCDLLVVAGARFDDRVTGRLATFANKAKVIHLD
CHHCCCCCCHHEEEECCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEE
IDAAELGKLRQPDVAIAGDLRQIFPALAMALNITPWQAEVEHLARKHQWDYQHPGSLIYA
CCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEH
PAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMWFRRPEDHLSSAGLGTMGFGLPAAIGAQ
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHCCH
VARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQKLGMVKQWQQLFFEERYS
HCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
ETDLSDNPDFVQLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLV
CCCCCCCCCCEEHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PPGASNSDMMDEMEKQT
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]