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The map label for this gene is tuf1 [H]

Identifier: 113968530

GI number: 113968530

Start: 211092

End: 212276

Strand: Direct

Name: tuf1 [H]

Synonym: Shewmr4_0185

Alternate gene names: 113968530

Gene position: 211092-212276 (Clockwise)

Preceding gene: 113968529

Following gene: 113968531

Centisome position: 4.49

GC content: 47.59

Gene sequence:

>1185_bases
ATGGCAAAAGCTAAATTTGAACGTAATAAGCCCCATGTTAACGTGGGCACCATCGGTCACGTTGACCATGGTAAAACCAC
TCTGACTGCAGCTATCTCTCACGTACTGGCTAAGACCTACGGTGGTGAAGCTAAAGACTTCTCTCAAATCGATAACGCTC
CAGAAGAGCGTGAGCGCGGTATTACCATCAATACCTCTCACATCGAATACGATACTCCAACTCGCCACTACGCACACGTA
GACTGCCCAGGCCACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCGATCCTGGTAGTAGC
TTCTACTGACGGTCCAATGCCACAAACTCGTGAGCACATCCTGCTGTCTCGCCAAGTAGGCGTACCATTCATCATCGTAT
TCATGAACAAGTGTGACATGGTTGACGATGCTGAACTGTTAGAATTAGTAGAAATGGAAGTACGTGAACTGTTATCAGAA
TACGACTTCCCAGGTGATGACTTACCAGTTATCCAAGGTTCTGCTCTGAAAGCGCTAGAAGGCGAGCCAGAGTGGGAAGC
TAAGATCATCGAATTAGCAGAAGCACTGGATTCTTACATTCCAGAACCAGAGCGTGATATCGATAAGCCATTCCTGATGC
CAATCGAAGACGTATTCTCAATCTCTGGCCGTGGTACAGTAGTAACTGGTCGTGTTGAGCGTGGTATCGTACGTGTTGGT
GACGAAGTAGAAATCGTTGGTATCCGTGCAACCACTAAGACAACCTGTACTGGTGTTGAAATGTTCCGTAAGCTGCTTGA
CGAAGGTCGTGCAGGTGAGAACTGTGGTATCCTGTTACGTGGTACTAAGCGTGATGACGTAGAACGTGGTCAAGTATTAT
CTAAGCCAGGCTCAATCAACCCACACACTACTTTTGAATCAGAAGTATACGTTCTGTCAAAAGAAGAAGGTGGTCGTCAC
ACTCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTGACTGGAACTATCGAACTGCCAGAAGG
CGTAGAGATGGTAATGCCAGGCGACAACATCAAGATGAAAGTCACCCTGATCTGCCCAATCGCGATGGACGAAGGTTTAC
GCTTCGCAATCCGTGAAGGTGGCCGTACAGTCGGTGCAGGTGTTGTAGCTAAGATTTTCGCTTAA

Upstream 100 bases:

>100_bases
GCTCCTATACCTAAATAATCGGTTCGATGTCATTTATATGCATCGGATTTTTTCTATATGTATGTTTTCATCACCAAGAC
TCTTGGATTGAGGCAATACC

Downstream 100 bases:

>100_bases
TCGCGAACTTAGATATAGTGCTGAAAAAGGAAGCTTAGGCTTCCTTTTTTGTTTAAGTCGATTATTTACTGGGTTTTGTG
GGCTGCAACTGTTGTGCATC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKIIELAEALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGIRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMKVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Sequences:

>Translated_394_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKIIELAEALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGIRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMKVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIFA
>Mature_393_residues
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGEPEWEAKIIELAEALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVGD
EVEIVGIRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMKVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI4503475, Length=449, Percent_Identity=29.3986636971047, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.3453724604966, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=29.3453724604966, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=28.5077951002227, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=29.2753623188406, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=29.4460641399417, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=29.4460641399417, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=29.4460641399417, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=28.8571428571429, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=357, Percent_Identity=27.4509803921569, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI82546879, Length=430, Percent_Identity=24.4186046511628, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI53729339, Length=232, Percent_Identity=31.4655172413793, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=232, Percent_Identity=31.4655172413793, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=250, Percent_Identity=25.2, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=86.2944162436548, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=86.2944162436548, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=28.9124668435013, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=30.5555555555556, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=27.9503105590062, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=46.1139896373057, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.7777777777778, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.7777777777778, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.2758620689655, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=27.6497695852535, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=27.6497695852535, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=30.6273062730627, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=25.4004576659039, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=447, Percent_Identity=26.1744966442953, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=152, Percent_Identity=36.1842105263158, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.0851063829787, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.4760705289673, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=29.9776286353468, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=29.9776286353468, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=23.1947483588621, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=28.7833827893175, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=27.8571428571429, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=30.1255230125523, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=57.3264781491003, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=57.3264781491003, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.5544041450777, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=27.6169265033408, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=27.6169265033408, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=27.6169265033408, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=27.6169265033408, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=27.8396436525612, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=27.8396436525612, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=30.1324503311258, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=29.2899408284024, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=30.7462686567164, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24647046, Length=366, Percent_Identity=26.775956284153, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24647048, Length=366, Percent_Identity=26.775956284153, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28572034, Length=163, Percent_Identity=36.1963190184049, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43417; Mature: 43286

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKIIELAEALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGIRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCEECCCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMK
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEEEEEECCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKIIELAEALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGIRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCEECCCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMK
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEEEEEECCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA