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The map label for this gene is hydA [H]

Identifier: 113476883

GI number: 113476883

Start: 5165518

End: 5166480

Strand: Reverse

Name: hydA [H]

Synonym: Tery_3369

Alternate gene names: 113476883

Gene position: 5166480-5165518 (Counterclockwise)

Preceding gene: 113476885

Following gene: 113476882

Centisome position: 66.66

GC content: 40.81

Gene sequence:

>963_bases
ATGATAAATCTACTGTGGCTTCAAGGTGGAGCTTGTTCTGGCAATACCATATCATTTCTCAATGCTGAAGAACCAAATAT
TTGTGATTTAATTACAGACTTCGGCATTAATGTACTTTGGCATCCTTCCCTAGGAATAGAACTTGGTACAAATGTTCAAC
AATTATTAAAAGATTGTATTTCCGGAAAAATACAATTAGATATTTTAGTATACGAAGGAACAGTAATTAACGCACCAAAT
GGTATGGGAGATTGGAACCGTTTTGCCGATCGGCCAATGAAAAACTGGGTAAAAGAATTAGCAGAAATTGCCAGTTTCGT
TGTTGCGGTAGGAGACTGCGCTACTTATGGCGGTATTCCGGCAATGGCACCAAACCCCAGTGAGTCTGAAGGTTTACAAT
TTCTGAGACGGAAAAAAGGTGGATTTTTAGGAGAAGACTTCAAAAGCCAAGCTGGATATCCTGTGATCAACATTCCTGGT
TGCCCTGCACACCCAGATTGGATTTCGCAAATATTGGTAGCAGTAGCCACTGGCAGACTTAATGACATTACCTTAGACGA
ATTTCACCGCCCTGAAACATTTTTCAAAAGCTTCACTCAAACAGGTTGTACTCGCAACGTTCACTTTGCTTACAAAGCTT
CAACCTCAGATTTTGGGCAACGTCAAGGATGTCTATTTTATGATTTAGGTTGTCGCGGACCGATGACTCGTTCTTCCTGT
AACCGCATTTTGTGGAACCGAGTTTCTTCTAAAACTCGAGCGGGGATGCCATGTTTAGGTTGCACGGAACCAGAATTTCC
ATTCCAGGATCTTATGCCTGGAACAGTATTTACAACTCAAACAGTTATGGGAGTGCCTAAAGAATTACCAACAGGAGTTA
ACCGTAAAGATTATGCTGTATTGACTATGGTTGCTAAAAATTCAACGCCAGCATGGGCAGAGGAAGATTTCTTTACAGTT
TAA

Upstream 100 bases:

>100_bases
ATCCTCTATAATTATATGTATATAGGTTGAATTAGGGTGCAACAAATTCTCTCGTGAATCCCATCTCAACCTCAGATAAC
AGAGAGGAGAATTAGAGAGA

Downstream 100 bases:

>100_bases
AATTTGTAGTTTGGTTAAAGAAACAAAACCCAACATAATTAGGAGTTATATCATATCCAGATAACTAGTGATCAAAGTGA
TCAAAAAGTATGTAGTTTGT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MINLLWLQGGACSGNTISFLNAEEPNICDLITDFGINVLWHPSLGIELGTNVQQLLKDCISGKIQLDILVYEGTVINAPN
GMGDWNRFADRPMKNWVKELAEIASFVVAVGDCATYGGIPAMAPNPSESEGLQFLRRKKGGFLGEDFKSQAGYPVINIPG
CPAHPDWISQILVAVATGRLNDITLDEFHRPETFFKSFTQTGCTRNVHFAYKASTSDFGQRQGCLFYDLGCRGPMTRSSC
NRILWNRVSSKTRAGMPCLGCTEPEFPFQDLMPGTVFTTQTVMGVPKELPTGVNRKDYAVLTMVAKNSTPAWAEEDFFTV

Sequences:

>Translated_320_residues
MINLLWLQGGACSGNTISFLNAEEPNICDLITDFGINVLWHPSLGIELGTNVQQLLKDCISGKIQLDILVYEGTVINAPN
GMGDWNRFADRPMKNWVKELAEIASFVVAVGDCATYGGIPAMAPNPSESEGLQFLRRKKGGFLGEDFKSQAGYPVINIPG
CPAHPDWISQILVAVATGRLNDITLDEFHRPETFFKSFTQTGCTRNVHFAYKASTSDFGQRQGCLFYDLGCRGPMTRSSC
NRILWNRVSSKTRAGMPCLGCTEPEFPFQDLMPGTVFTTQTVMGVPKELPTGVNRKDYAVLTMVAKNSTPAWAEEDFFTV
>Mature_320_residues
MINLLWLQGGACSGNTISFLNAEEPNICDLITDFGINVLWHPSLGIELGTNVQQLLKDCISGKIQLDILVYEGTVINAPN
GMGDWNRFADRPMKNWVKELAEIASFVVAVGDCATYGGIPAMAPNPSESEGLQFLRRKKGGFLGEDFKSQAGYPVINIPG
CPAHPDWISQILVAVATGRLNDITLDEFHRPETFFKSFTQTGCTRNVHFAYKASTSDFGQRQGCLFYDLGCRGPMTRSSC
NRILWNRVSSKTRAGMPCLGCTEPEFPFQDLMPGTVFTTQTVMGVPKELPTGVNRKDYAVLTMVAKNSTPAWAEEDFFTV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=271, Percent_Identity=30.9963099630996, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789371, Length=272, Percent_Identity=26.8382352941176, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 35227; Mature: 35227

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLLWLQGGACSGNTISFLNAEEPNICDLITDFGINVLWHPSLGIELGTNVQQLLKDCI
CEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEECCCCCCEECCCHHHHHHHHH
SGKIQLDILVYEGTVINAPNGMGDWNRFADRPMKNWVKELAEIASFVVAVGDCATYGGIP
CCCEEEEEEEEECEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AMAPNPSESEGLQFLRRKKGGFLGEDFKSQAGYPVINIPGCPAHPDWISQILVAVATGRL
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCC
NDITLDEFHRPETFFKSFTQTGCTRNVHFAYKASTSDFGQRQGCLFYDLGCRGPMTRSSC
CCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHH
NRILWNRVSSKTRAGMPCLGCTEPEFPFQDLMPGTVFTTQTVMGVPKELPTGVNRKDYAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEHHHHHHCCCHHCCCCCCCCCEEE
LTMVAKNSTPAWAEEDFFTV
EEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MINLLWLQGGACSGNTISFLNAEEPNICDLITDFGINVLWHPSLGIELGTNVQQLLKDCI
CEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEECCCCCCEECCCHHHHHHHHH
SGKIQLDILVYEGTVINAPNGMGDWNRFADRPMKNWVKELAEIASFVVAVGDCATYGGIP
CCCEEEEEEEEECEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AMAPNPSESEGLQFLRRKKGGFLGEDFKSQAGYPVINIPGCPAHPDWISQILVAVATGRL
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCC
NDITLDEFHRPETFFKSFTQTGCTRNVHFAYKASTSDFGQRQGCLFYDLGCRGPMTRSSC
CCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHH
NRILWNRVSSKTRAGMPCLGCTEPEFPFQDLMPGTVFTTQTVMGVPKELPTGVNRKDYAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEHHHHHHCCCHHCCCCCCCCCEEE
LTMVAKNSTPAWAEEDFFTV
EEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 3134950; 7854413 [H]