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The map label for this gene is hybC [C]

Identifier: 113476882

GI number: 113476882

Start: 5163224

End: 5164828

Strand: Reverse

Name: hybC [C]

Synonym: Tery_3368

Alternate gene names: 113476882

Gene position: 5164828-5163224 (Counterclockwise)

Preceding gene: 113476883

Following gene: 113476881

Centisome position: 66.64

GC content: 43.8

Gene sequence:

>1605_bases
ATGGTTCAAACTAAAGTACAAACATTAGACATTTCCCCCGTGGGTAGGGTTGAAGGCGACCTAGATGTAAGAGTTGATAT
TCAAGACGGATATGTAAGCAACGCTTGGACTCAAGCTCAAATGTTTCGAGGTTTTGAAGTCATCCTCAAAGGCAAAGACC
CTAAAGCTGGCCTCATCGTCACTCCCAGAATTTGTGGAATTTGTGGTGCTTCTCACCTCACCTGTGCTTCCTGGGCATTA
GATACGGCTTGGCAAACGGAAGTACCCCGTAACGCGATCTTGGCTCGTAACCTTGGTCAACTAGTGGAAACACTTCAAAG
TCATCCACGCTACTTTTATGGGTTATACGCCATCGACTTAACAAACAAAAAATACCAAAATAGTCCTTTCTACGAAGAAG
CTTGCAAGCGTTTTACAGCATTCACAGGTACATCCTATGAACCAGGTATTACTGTAGCAGCAAAACCCGTAGAAATATAT
GCTCTATTTGGTGGTCAATGGCCTCACTCTAGTTTCATGGTTCCTGGTGGTGTTATGTGTGCTCCCACTCTCACCGATGT
GACCCGCGCCTGGGGAATGTTGGAATATTTCCGCACTAACTGGTTAGAACCGTTATGGTTAGGTTGTTCCATTGAGAGGT
TTGAAAAAATCCAAACTTATGAAGATTTTCTGGAATGGTTAGATGAAAATCCTCATCATGCCAGTTCAGACCTTGGTTTC
TACTGGCGCATGGGTCTGAATATTGGTTTGGATAAAATTGGAGCTGGTGTTGGTAAATATATGAGTTGGGGGTATTTGCC
TCACGAAGACAAATATCAACACCCGACTATTGAAGGTCGGAATGCTGCTATGATTATGAAAAGTGGTGTCTATGATAGCA
CTACAGATATTCATCAATTGATGGAGCATACTTTTACCCGGGAGAATACTAGCCACGCATGGTATAATGAGGGTGATGAG
AATATACATCCTTTTGACCGGAATACTGCACCTATTCGTAATAATGACCTTGACTTTGATGGTGCTTATTCTTGGTCAAC
TGCTGTATCTCACAAAGACTTAGGTCGGATGGAAGTTGGGGCTTTGGCACGGGAACTAATAGCAGGTGGCGACCATGGGG
AAAGTTGGCAGCATAAAGATGGTTTAGTTTTGGATATGTTCAAGAAAATGGGCGGACCGAGCGTACATTTACGTGTGTTT
GCCCGAATGCACGAATTAACTAAGTTGTATCGTCAAACAGAACATTGTTTGCGGGAGTTTAATTTGCGCGATCCTTGGTA
TATTAAGCCTCAAGAAAAAGATGGTCGTGGTTGGGGTGCTACTAATGCTAGTCGTGGTTCTCTCTGTCATTGGGTGGAAA
TAGAAGGGGGTAAGATTAATAATTATCAAATTATTGCACCGACTACTTGGAATGTAGGACCTCGTGATGGTCAAGGGGTG
CGTGGACCTATTGAGGAAGCGTTAGTTGGTATTCCTATTGCTGACGTGAATGATCCGGTGGAAGTCGGTCATGTTGCTCG
CTCTTTTGATTCTTGTTTGGTGTGTACAGTTCATGCTCATGATGCTAAGACAGGAGAAGAGCTAGCGCGGTTCCGGACTT
CTTAG

Upstream 100 bases:

>100_bases
TTTCATGTTGCGTAGCAAAACCTAACCAAAACAGACTGTAGCTACTGATAAATTATGAGTGATGACTAATAACTGTATTA
TTCTGGAGGTTTAACAAAAA

Downstream 100 bases:

>100_bases
ATATTACTTCAGTATGGTTTTTTAATCAGGACTTACGCCATAAGGTAAGATTATACAATGTTTGTAGAAGTTAAGGGCTA
CTCATTATATATGGTTGTTA

Product: hydrogen:quinone oxidoreductase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MVQTKVQTLDISPVGRVEGDLDVRVDIQDGYVSNAWTQAQMFRGFEVILKGKDPKAGLIVTPRICGICGASHLTCASWAL
DTAWQTEVPRNAILARNLGQLVETLQSHPRYFYGLYAIDLTNKKYQNSPFYEEACKRFTAFTGTSYEPGITVAAKPVEIY
ALFGGQWPHSSFMVPGGVMCAPTLTDVTRAWGMLEYFRTNWLEPLWLGCSIERFEKIQTYEDFLEWLDENPHHASSDLGF
YWRMGLNIGLDKIGAGVGKYMSWGYLPHEDKYQHPTIEGRNAAMIMKSGVYDSTTDIHQLMEHTFTRENTSHAWYNEGDE
NIHPFDRNTAPIRNNDLDFDGAYSWSTAVSHKDLGRMEVGALARELIAGGDHGESWQHKDGLVLDMFKKMGGPSVHLRVF
ARMHELTKLYRQTEHCLREFNLRDPWYIKPQEKDGRGWGATNASRGSLCHWVEIEGGKINNYQIIAPTTWNVGPRDGQGV
RGPIEEALVGIPIADVNDPVEVGHVARSFDSCLVCTVHAHDAKTGEELARFRTS

Sequences:

>Translated_534_residues
MVQTKVQTLDISPVGRVEGDLDVRVDIQDGYVSNAWTQAQMFRGFEVILKGKDPKAGLIVTPRICGICGASHLTCASWAL
DTAWQTEVPRNAILARNLGQLVETLQSHPRYFYGLYAIDLTNKKYQNSPFYEEACKRFTAFTGTSYEPGITVAAKPVEIY
ALFGGQWPHSSFMVPGGVMCAPTLTDVTRAWGMLEYFRTNWLEPLWLGCSIERFEKIQTYEDFLEWLDENPHHASSDLGF
YWRMGLNIGLDKIGAGVGKYMSWGYLPHEDKYQHPTIEGRNAAMIMKSGVYDSTTDIHQLMEHTFTRENTSHAWYNEGDE
NIHPFDRNTAPIRNNDLDFDGAYSWSTAVSHKDLGRMEVGALARELIAGGDHGESWQHKDGLVLDMFKKMGGPSVHLRVF
ARMHELTKLYRQTEHCLREFNLRDPWYIKPQEKDGRGWGATNASRGSLCHWVEIEGGKINNYQIIAPTTWNVGPRDGQGV
RGPIEEALVGIPIADVNDPVEVGHVARSFDSCLVCTVHAHDAKTGEELARFRTS
>Mature_534_residues
MVQTKVQTLDISPVGRVEGDLDVRVDIQDGYVSNAWTQAQMFRGFEVILKGKDPKAGLIVTPRICGICGASHLTCASWAL
DTAWQTEVPRNAILARNLGQLVETLQSHPRYFYGLYAIDLTNKKYQNSPFYEEACKRFTAFTGTSYEPGITVAAKPVEIY
ALFGGQWPHSSFMVPGGVMCAPTLTDVTRAWGMLEYFRTNWLEPLWLGCSIERFEKIQTYEDFLEWLDENPHHASSDLGF
YWRMGLNIGLDKIGAGVGKYMSWGYLPHEDKYQHPTIEGRNAAMIMKSGVYDSTTDIHQLMEHTFTRENTSHAWYNEGDE
NIHPFDRNTAPIRNNDLDFDGAYSWSTAVSHKDLGRMEVGALARELIAGGDHGESWQHKDGLVLDMFKKMGGPSVHLRVF
ARMHELTKLYRQTEHCLREFNLRDPWYIKPQEKDGRGWGATNASRGSLCHWVEIEGGKINNYQIIAPTTWNVGPRDGQGV
RGPIEEALVGIPIADVNDPVEVGHVARSFDSCLVCTVHAHDAKTGEELARFRTS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=587, Percent_Identity=27.0868824531516, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787207, Length=590, Percent_Identity=26.4406779661017, Blast_Score=165, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 60199; Mature: 60199

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTKVQTLDISPVGRVEGDLDVRVDIQDGYVSNAWTQAQMFRGFEVILKGKDPKAGLIV
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCEEE
TPRICGICGASHLTCASWALDTAWQTEVPRNAILARNLGQLVETLQSHPRYFYGLYAIDL
CCHHHHHCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
TNKKYQNSPFYEEACKRFTAFTGTSYEPGITVAAKPVEIYALFGGQWPHSSFMVPGGVMC
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCCCCCCEECCCCEEE
APTLTDVTRAWGMLEYFRTNWLEPLWLGCSIERFEKIQTYEDFLEWLDENPHHASSDLGF
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
YWRMGLNIGLDKIGAGVGKYMSWGYLPHEDKYQHPTIEGRNAAMIMKSGVYDSTTDIHQL
EEEECCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
MEHTFTRENTSHAWYNEGDENIHPFDRNTAPIRNNDLDFDGAYSWSTAVSHKDLGRMEVG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHHHH
ALARELIAGGDHGESWQHKDGLVLDMFKKMGGPSVHLRVFARMHELTKLYRQTEHCLREF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
NLRDPWYIKPQEKDGRGWGATNASRGSLCHWVEIEGGKINNYQIIAPTTWNVGPRDGQGV
CCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCEEEEECCCCCCCCCCCCCC
RGPIEEALVGIPIADVNDPVEVGHVARSFDSCLVCTVHAHDAKTGEELARFRTS
CCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCC
>Mature Secondary Structure
MVQTKVQTLDISPVGRVEGDLDVRVDIQDGYVSNAWTQAQMFRGFEVILKGKDPKAGLIV
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCEEE
TPRICGICGASHLTCASWALDTAWQTEVPRNAILARNLGQLVETLQSHPRYFYGLYAIDL
CCHHHHHCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
TNKKYQNSPFYEEACKRFTAFTGTSYEPGITVAAKPVEIYALFGGQWPHSSFMVPGGVMC
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCCCCCCEECCCCEEE
APTLTDVTRAWGMLEYFRTNWLEPLWLGCSIERFEKIQTYEDFLEWLDENPHHASSDLGF
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
YWRMGLNIGLDKIGAGVGKYMSWGYLPHEDKYQHPTIEGRNAAMIMKSGVYDSTTDIHQL
EEEECCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
MEHTFTRENTSHAWYNEGDENIHPFDRNTAPIRNNDLDFDGAYSWSTAVSHKDLGRMEVG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHHHH
ALARELIAGGDHGESWQHKDGLVLDMFKKMGGPSVHLRVFARMHELTKLYRQTEHCLREF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
NLRDPWYIKPQEKDGRGWGATNASRGSLCHWVEIEGGKINNYQIIAPTTWNVGPRDGQGV
CCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCEEEEECCCCCCCCCCCCCC
RGPIEEALVGIPIADVNDPVEVGHVARSFDSCLVCTVHAHDAKTGEELARFRTS
CCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]