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The map label for this gene is cobS [H]

Identifier: 113476082

GI number: 113476082

Start: 3782982

End: 3783746

Strand: Direct

Name: cobS [H]

Synonym: Tery_2458

Alternate gene names: 113476082

Gene position: 3782982-3783746 (Clockwise)

Preceding gene: 113476079

Following gene: 113476085

Centisome position: 48.81

GC content: 39.61

Gene sequence:

>765_bases
ATGCAACTAATTAGAGAAACTCTTGCCAAGTTCATGGCTGGAATTGCTTTTTACACCTGTTTACCAATTCCCCATGATTG
GAATTTAGATTTTCACGGTATTGCTCGTTTAGCTCCATTGATCGGATTGATACTTGGAGGTATTCAAGGTTTAGTAGATA
TAGGATTACATTCTTTAGGAATGCCTGTACTCACTAGATCAACTGTAGTGATTATATCTGGAATTGCTTTAACTGGCGGT
TTACATTTGGATGGGGTAATGGATGCTGCTGATGGGTTAGCGGTACCAGAAAAAAAACGAAGACTCGAAGTAATGACAGA
TAGCGCTACAGGAGCTTTTGGTGCAATGGCAGCGATCGCTCTACTACTCATCAAAATTACAGCTTTAACAGATTTAGACA
GCGATCGCCTTTTAATACTAATGGGAATAGCAGGTTGGGGGAGATGGGGTCAGTTAGTAGCGATCGCTTGTTACCCCTAT
CTTAAACCTACTGGAAAAGGAGCATTTCACAAAAAAGCAAAATATTCTATTTTGGATTTTTTACTAGGAATACTCTTATT
ACTGCTTATGAGTGGAATTCTTACTTTATTAGATCCTCAAAATTGGTTGGTAGCTTGTGGAATGGCAATAGGAGGAATAG
CGATCGCTCTACTAACAGGTGCTTGGTTTAACCGTTATTTGGGCGGTCATACTGGCGACACTTACGGCGCTGTAGTTGAA
TGGACAGAAGCATTATTACTATGCGTTGTAGTTACTTTAGAATAA

Upstream 100 bases:

>100_bases
GGTTGATCGGTTTTCCCGCTATGAGGCTCCCTTATAGAAGAGGGCGAGTCTTCAACCAACTTCAGGATCATAAATCATAA
ATTTGCGACGGTAAATATTG

Downstream 100 bases:

>100_bases
AGGATTGGGTTTTTGTGAAAAAATTTGAACAGAAAACTTACCCTAAACAAAACTATCACAATTTGAAAAAAACTGCTCAG
GTCGGAACCTCCTGTATTTT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQLIRETLAKFMAGIAFYTCLPIPHDWNLDFHGIARLAPLIGLILGGIQGLVDIGLHSLGMPVLTRSTVVIISGIALTGG
LHLDGVMDAADGLAVPEKKRRLEVMTDSATGAFGAMAAIALLLIKITALTDLDSDRLLILMGIAGWGRWGQLVAIACYPY
LKPTGKGAFHKKAKYSILDFLLGILLLLLMSGILTLLDPQNWLVACGMAIGGIAIALLTGAWFNRYLGGHTGDTYGAVVE
WTEALLLCVVVTLE

Sequences:

>Translated_254_residues
MQLIRETLAKFMAGIAFYTCLPIPHDWNLDFHGIARLAPLIGLILGGIQGLVDIGLHSLGMPVLTRSTVVIISGIALTGG
LHLDGVMDAADGLAVPEKKRRLEVMTDSATGAFGAMAAIALLLIKITALTDLDSDRLLILMGIAGWGRWGQLVAIACYPY
LKPTGKGAFHKKAKYSILDFLLGILLLLLMSGILTLLDPQNWLVACGMAIGGIAIALLTGAWFNRYLGGHTGDTYGAVVE
WTEALLLCVVVTLE
>Mature_254_residues
MQLIRETLAKFMAGIAFYTCLPIPHDWNLDFHGIARLAPLIGLILGGIQGLVDIGLHSLGMPVLTRSTVVIISGIALTGG
LHLDGVMDAADGLAVPEKKRRLEVMTDSATGAFGAMAAIALLLIKITALTDLDSDRLLILMGIAGWGRWGQLVAIACYPY
LKPTGKGAFHKKAKYSILDFLLGILLLLLMSGILTLLDPQNWLVACGMAIGGIAIALLTGAWFNRYLGGHTGDTYGAVVE
WTEALLLCVVVTLE

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=34.6774193548387, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27049; Mature: 27049

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIRETLAKFMAGIAFYTCLPIPHDWNLDFHGIARLAPLIGLILGGIQGLVDIGLHSLG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPVLTRSTVVIISGIALTGGLHLDGVMDAADGLAVPEKKRRLEVMTDSATGAFGAMAAIA
CCHHHCCCEEEEEHHHHCCCCCCCHHHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHH
LLLIKITALTDLDSDRLLILMGIAGWGRWGQLVAIACYPYLKPTGKGAFHKKAKYSILDF
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHEEHEEEECCCCCCCCCCHHHHHHHHHHHH
LLGILLLLLMSGILTLLDPQNWLVACGMAIGGIAIALLTGAWFNRYLGGHTGDTYGAVVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
WTEALLLCVVVTLE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MQLIRETLAKFMAGIAFYTCLPIPHDWNLDFHGIARLAPLIGLILGGIQGLVDIGLHSLG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPVLTRSTVVIISGIALTGGLHLDGVMDAADGLAVPEKKRRLEVMTDSATGAFGAMAAIA
CCHHHCCCEEEEEHHHHCCCCCCCHHHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHH
LLLIKITALTDLDSDRLLILMGIAGWGRWGQLVAIACYPYLKPTGKGAFHKKAKYSILDF
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHEEHEEEECCCCCCCCCCHHHHHHHHHHHH
LLGILLLLLMSGILTLLDPQNWLVACGMAIGGIAIALLTGAWFNRYLGGHTGDTYGAVVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
WTEALLLCVVVTLE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA