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The map label for this gene is pncA [C]

Identifier: 113476076

GI number: 113476076

Start: 3777196

End: 3777939

Strand: Direct

Name: pncA [C]

Synonym: Tery_2452

Alternate gene names: 113476076

Gene position: 3777196-3777939 (Clockwise)

Preceding gene: 113476075

Following gene: 113476077

Centisome position: 48.74

GC content: 41.4

Gene sequence:

>744_bases
ATGACTACAACTCTAGCTACTCCTGATACTAGTTTAGCAACCATTCAAGCTGCTTTACCTATTGATCCACAGCCCTATAT
TATAGCCGATCGCCCTACCGGACTCATAGTCGTAGATGTACTTAATGGTTTTTGCACTGTTGGTTTTGGACCATTAGCCC
CCCAAGAGCCAAACGAACAAATCGCTACAATGGTCTCGGAAAGCGATCGCCTAGCTCGTACTTTTGTAGAAAAAGGTTGG
CCTGTATTAGCCTTTTTAGATACCCATGAACCAGGAAAACCCGAGCCTCCTTATCCACCACATTGTGAAAAGGGTACGGG
AGAAGAAGAACTCGTTCCCGAACTTCAATGGTTACACGATAACCCCCTGGCAACATTGGTTTTTAAGGATTGTATTAATG
GATTTATAGGTTCTATTGACATAGATACCCAAGGTAATGTATTGTTGGATTGGATAAATAAGAATAAGCTTGAGGCCCTT
GTTGTAGTTGGAATTTGTACTGATATCTGTGTCATGGACTTTGTTGTTACTATTCTGTCAGTACGCAATCATGGTTTAGC
GCCTACCTTAAAAGATGTGGCTGTATACGATCAAGGTTGTGCTACTTTTGATATGACAGCACAAATGGCAGCCGAGAAAG
GCTTACCCAAAACTGCTATTCATCCACAGAAAATATCTCATCATGTAGGGTTATATACAATGGCCGAACGTGGTGCATTT
ATTGCTTCTACTATTAAGCTTTAG

Upstream 100 bases:

>100_bases
AAAATAACTATAAGTACTAACAAAAAATTAGTCCTTCCCGATTCAAAACTGATGAGATTTGAACAGAAAATCTCTTGAAA
AAATAAGGATTAAAATAAAT

Downstream 100 bases:

>100_bases
AAGCTGATTCACTTAACTAGCTATTAGCTTTTAGCAGTCCTAGTTGAAATCACTAGTTTCTATAAACCGAAAGGTTTAAG
AGAGTTTTAAATACCCTTCT

Product: isochorismatase hydrolase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTTTLATPDTSLATIQAALPIDPQPYIIADRPTGLIVVDVLNGFCTVGFGPLAPQEPNEQIATMVSESDRLARTFVEKGW
PVLAFLDTHEPGKPEPPYPPHCEKGTGEEELVPELQWLHDNPLATLVFKDCINGFIGSIDIDTQGNVLLDWINKNKLEAL
VVVGICTDICVMDFVVTILSVRNHGLAPTLKDVAVYDQGCATFDMTAQMAAEKGLPKTAIHPQKISHHVGLYTMAERGAF
IASTIKL

Sequences:

>Translated_247_residues
MTTTLATPDTSLATIQAALPIDPQPYIIADRPTGLIVVDVLNGFCTVGFGPLAPQEPNEQIATMVSESDRLARTFVEKGW
PVLAFLDTHEPGKPEPPYPPHCEKGTGEEELVPELQWLHDNPLATLVFKDCINGFIGSIDIDTQGNVLLDWINKNKLEAL
VVVGICTDICVMDFVVTILSVRNHGLAPTLKDVAVYDQGCATFDMTAQMAAEKGLPKTAIHPQKISHHVGLYTMAERGAF
IASTIKL
>Mature_246_residues
TTTLATPDTSLATIQAALPIDPQPYIIADRPTGLIVVDVLNGFCTVGFGPLAPQEPNEQIATMVSESDRLARTFVEKGWP
VLAFLDTHEPGKPEPPYPPHCEKGTGEEELVPELQWLHDNPLATLVFKDCINGFIGSIDIDTQGNVLLDWINKNKLEALV
VVGICTDICVMDFVVTILSVRNHGLAPTLKDVAVYDQGCATFDMTAQMAAEKGLPKTAIHPQKISHHVGLYTMAERGAFI
ASTIKL

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26800; Mature: 26669

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTLATPDTSLATIQAALPIDPQPYIIADRPTGLIVVDVLNGFCTVGFGPLAPQEPNEQ
CCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCCCCCCCCHH
IATMVSESDRLARTFVEKGWPVLAFLDTHEPGKPEPPYPPHCEKGTGEEELVPELQWLHD
HHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NPLATLVFKDCINGFIGSIDIDTQGNVLLDWINKNKLEALVVVGICTDICVMDFVVTILS
CCHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHH
VRNHGLAPTLKDVAVYDQGCATFDMTAQMAAEKGLPKTAIHPQKISHHVGLYTMAERGAF
HHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEEEHHCCCE
IASTIKL
EEEEECC
>Mature Secondary Structure 
TTTLATPDTSLATIQAALPIDPQPYIIADRPTGLIVVDVLNGFCTVGFGPLAPQEPNEQ
CCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCCCCCCCCHH
IATMVSESDRLARTFVEKGWPVLAFLDTHEPGKPEPPYPPHCEKGTGEEELVPELQWLHD
HHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NPLATLVFKDCINGFIGSIDIDTQGNVLLDWINKNKLEALVVVGICTDICVMDFVVTILS
CCHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHH
VRNHGLAPTLKDVAVYDQGCATFDMTAQMAAEKGLPKTAIHPQKISHHVGLYTMAERGAF
HHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEEEHHCCCE
IASTIKL
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA