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The map label for this gene is fadD21 [H]

Identifier: 113475635

GI number: 113475635

Start: 3060023

End: 3061867

Strand: Direct

Name: fadD21 [H]

Synonym: Tery_1972

Alternate gene names: 113475635

Gene position: 3060023-3061867 (Clockwise)

Preceding gene: 113475633

Following gene: 113475636

Centisome position: 39.48

GC content: 38.81

Gene sequence:

>1845_bases
ATGAGTAGTTTAAATAAAAAAATTAAAACATTAGTAGATTTATTACAACATAGAGCTATCCAAAACCCGGAAAAAATAGG
TTACAGCTTTTTAATAGATGGAGAAATAGAAACAGTTAACCTTACCTATAAACAACTGGAACAACGTTCACGGGCGATCG
CTGCCTATCTCCAGTCAGTATGTCCACAAGGAGAAGTTGCCCTACTCCTCTATCAACCGGGACTAGAATATATTACCACT
TTTTTTGGGTGTCTTTATGCAGGGGTTATAGCAATTCCTGCCTACCCCCCTAGGCCAAATCGTTCCCTAGACCGGATCCA
AACAATTATTAGAGATAGTAAGGCCAAAATAGCTTTGACAACTAAATCTATCATTTCTAGCCTAGAACCTCGTGCCTCAG
AAACTCCAGAATTAGAGAATTTAGGTTGGTTAGCTACTGACCAAATTCCGGACTATTTTGCAGATCAATGGCTTGAACCG
AATATTAATGAGAATACCCTTGCTTTTCTCCAATATACTTCTGGTTCGACAGCTAAACCTAAAGGGGTAATGATTACCCA
CCAGAACCTTTTGCATAATTCTGGTCTTATTCATCAATGTTTTGGACACTCTGGTGAAAGTAAAGGAGTAATTTGGCTAC
CTCCTTATCATGACATGGGTTTAATTGGTGGAATATTGCAACCCTTATATGGGGCTTTTCCTGTCATTCTCATGTCTCCT
TTGATGTTTCTCCAAAGTCCAGTGCGCTGGCTAAAAGCTATTTCTCGGTACCAAGGTACTACTAGTGGGGGACCAAATTT
TGCTTATGATTTGTGTGTCCGTAAAATTAAGCCTGAACAGATACAAACTTTAGACTTAAGTAGTTGGGAAGTAGCTTTTA
ATGGTGCAGAACCTATTAGTGCTGAAGTTTTAGAACGATTTACTAATACTTTTTCTGTATGTGGTTTTCGCAGAGAAGCT
TTTTATCCTTGTTATGGGATGGCTGAGGCTACTTTAATTATTTCTGGGGGTAATAAGTTTTCTCCGCCGGTAGAAACTAA
GGTTGCAGCTAGGGCATTGGAAAAAAACCAGATTGTTTTAGTTTACCCAACAGAAGGAACAAAAACATTAGTGGGTTGTG
GTCAAACTTTGCCAGAGCAACAGATTAAGATTGTTCATCCAGAAAAATTAACTCTTTGTACTGAGGGAGAGGTAGGAGAG
ATTTGGGTCTCTGGCCCAAGTATTGCTGGGGGTTATTGGCAAAAACCAGAAGCAACGAAGCAGATTTTTGCTGCTTATTT
AGCTGAAGCACCCGGAAAAGAGCCATTTATGAGAACTGGAGATTTAGGCTTTCTGGAGGCTGGGGAATTATTTGTGACGG
GTCGCCTTAAGGATGTAATTATTATTAATGGTCGAAATCATTACCCTCAAGATATTGAATGGACAGTGGAGCAAAGCCAT
CCCCTGATTAGACCAGGTTGTATTGCTAGCTTTTCTGTAGAAGTTGGAGGAGAAGAACGTTTGGTAGTAGTAGCTGAGGT
AGAACGGAAATTTCGTCAACTCCTTGAGAGAAGTCAAAAGTCAAAAATAAAGAGTCAAAAATATGAATCTACTGATAAAT
CTGTGGGCTCGGTAGCAGTTGGTTTTTTCAGTGAGCGTAATGGTAAGTCTGAGAATAAAAATTCTGATAATTCTCAAGAC
TTAATTAAATCTATCCAAAGGTCTGTGTCAATAAATCACGATTTACAGATTTATCAAATATTATTGCTTAAACCGGGAGC
TATTCCCAAAACTTCTAGTGGAAAAGTCCAGCGTTATGCTTGTCGCGAAGGTTTTTTAGCTGGAACTCTAAAAATATTTG
ATTAA

Upstream 100 bases:

>100_bases
AGCTTTTTTTATTACTTCTTATTTCTAATAATCTGTATATTTGCGTTAAAATTTGTGTCGTGCCTTTCTAATTATTTTTG
TGTTTTTATATCAAAATCAA

Downstream 100 bases:

>100_bases
AGAAAATGTTTAGGGGCTACATACAAATCAGAATTCAGAAGCCACAAGGAAGGGGCAAAGTTAGAGCTTAGAAGTATTCC
CTAAGTGGGCTTGAGGATTT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSSLNKKIKTLVDLLQHRAIQNPEKIGYSFLIDGEIETVNLTYKQLEQRSRAIAAYLQSVCPQGEVALLLYQPGLEYITT
FFGCLYAGVIAIPAYPPRPNRSLDRIQTIIRDSKAKIALTTKSIISSLEPRASETPELENLGWLATDQIPDYFADQWLEP
NINENTLAFLQYTSGSTAKPKGVMITHQNLLHNSGLIHQCFGHSGESKGVIWLPPYHDMGLIGGILQPLYGAFPVILMSP
LMFLQSPVRWLKAISRYQGTTSGGPNFAYDLCVRKIKPEQIQTLDLSSWEVAFNGAEPISAEVLERFTNTFSVCGFRREA
FYPCYGMAEATLIISGGNKFSPPVETKVAARALEKNQIVLVYPTEGTKTLVGCGQTLPEQQIKIVHPEKLTLCTEGEVGE
IWVSGPSIAGGYWQKPEATKQIFAAYLAEAPGKEPFMRTGDLGFLEAGELFVTGRLKDVIIINGRNHYPQDIEWTVEQSH
PLIRPGCIASFSVEVGGEERLVVVAEVERKFRQLLERSQKSKIKSQKYESTDKSVGSVAVGFFSERNGKSENKNSDNSQD
LIKSIQRSVSINHDLQIYQILLLKPGAIPKTSSGKVQRYACREGFLAGTLKIFD

Sequences:

>Translated_614_residues
MSSLNKKIKTLVDLLQHRAIQNPEKIGYSFLIDGEIETVNLTYKQLEQRSRAIAAYLQSVCPQGEVALLLYQPGLEYITT
FFGCLYAGVIAIPAYPPRPNRSLDRIQTIIRDSKAKIALTTKSIISSLEPRASETPELENLGWLATDQIPDYFADQWLEP
NINENTLAFLQYTSGSTAKPKGVMITHQNLLHNSGLIHQCFGHSGESKGVIWLPPYHDMGLIGGILQPLYGAFPVILMSP
LMFLQSPVRWLKAISRYQGTTSGGPNFAYDLCVRKIKPEQIQTLDLSSWEVAFNGAEPISAEVLERFTNTFSVCGFRREA
FYPCYGMAEATLIISGGNKFSPPVETKVAARALEKNQIVLVYPTEGTKTLVGCGQTLPEQQIKIVHPEKLTLCTEGEVGE
IWVSGPSIAGGYWQKPEATKQIFAAYLAEAPGKEPFMRTGDLGFLEAGELFVTGRLKDVIIINGRNHYPQDIEWTVEQSH
PLIRPGCIASFSVEVGGEERLVVVAEVERKFRQLLERSQKSKIKSQKYESTDKSVGSVAVGFFSERNGKSENKNSDNSQD
LIKSIQRSVSINHDLQIYQILLLKPGAIPKTSSGKVQRYACREGFLAGTLKIFD
>Mature_613_residues
SSLNKKIKTLVDLLQHRAIQNPEKIGYSFLIDGEIETVNLTYKQLEQRSRAIAAYLQSVCPQGEVALLLYQPGLEYITTF
FGCLYAGVIAIPAYPPRPNRSLDRIQTIIRDSKAKIALTTKSIISSLEPRASETPELENLGWLATDQIPDYFADQWLEPN
INENTLAFLQYTSGSTAKPKGVMITHQNLLHNSGLIHQCFGHSGESKGVIWLPPYHDMGLIGGILQPLYGAFPVILMSPL
MFLQSPVRWLKAISRYQGTTSGGPNFAYDLCVRKIKPEQIQTLDLSSWEVAFNGAEPISAEVLERFTNTFSVCGFRREAF
YPCYGMAEATLIISGGNKFSPPVETKVAARALEKNQIVLVYPTEGTKTLVGCGQTLPEQQIKIVHPEKLTLCTEGEVGEI
WVSGPSIAGGYWQKPEATKQIFAAYLAEAPGKEPFMRTGDLGFLEAGELFVTGRLKDVIIINGRNHYPQDIEWTVEQSHP
LIRPGCIASFSVEVGGEERLVVVAEVERKFRQLLERSQKSKIKSQKYESTDKSVGSVAVGFFSERNGKSENKNSDNSQDL
IKSIQRSVSINHDLQIYQILLLKPGAIPKTSSGKVQRYACREGFLAGTLKIFD

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=541, Percent_Identity=26.9870609981516, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI225735629, Length=539, Percent_Identity=25.7884972170686, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI45827692, Length=539, Percent_Identity=25.7884972170686, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI55749758, Length=509, Percent_Identity=25.147347740668, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI225735625, Length=640, Percent_Identity=24.375, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI45827694, Length=536, Percent_Identity=25.5597014925373, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI225735627, Length=510, Percent_Identity=24.5098039215686, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI45827696, Length=510, Percent_Identity=24.5098039215686, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI187761345, Length=483, Percent_Identity=23.3954451345756, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI187761343, Length=483, Percent_Identity=23.3954451345756, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45827698, Length=472, Percent_Identity=24.5762711864407, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=25.8188824662813, Blast_Score=138, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI193202679, Length=674, Percent_Identity=22.9970326409496, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=23.5408560311284, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=23.3009708737864, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=479, Percent_Identity=22.1294363256785, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654656, Length=469, Percent_Identity=25.3731343283582, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI18859661, Length=349, Percent_Identity=27.2206303724928, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 68189; Mature: 68058

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLNKKIKTLVDLLQHRAIQNPEKIGYSFLIDGEIETVNLTYKQLEQRSRAIAAYLQSV
CCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHH
CPQGEVALLLYQPGLEYITTFFGCLYAGVIAIPAYPPRPNRSLDRIQTIIRDSKAKIALT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCEEEEE
TKSIISSLEPRASETPELENLGWLATDQIPDYFADQWLEPNINENTLAFLQYTSGSTAKP
HHHHHHHCCCCCCCCCCHHHCCCEECCCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
KGVMITHQNLLHNSGLIHQCFGHSGESKGVIWLPPYHDMGLIGGILQPLYGAFPVILMSP
CCEEEEEHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LMFLQSPVRWLKAISRYQGTTSGGPNFAYDLCVRKIKPEQIQTLDLSSWEVAFNGAEPIS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCH
AEVLERFTNTFSVCGFRREAFYPCYGMAEATLIISGGNKFSPPVETKVAARALEKNQIVL
HHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEE
VYPTEGTKTLVGCGQTLPEQQIKIVHPEKLTLCTEGEVGEIWVSGPSIAGGYWQKPEATK
EECCCCCEEEEECCCCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCHHHH
QIFAAYLAEAPGKEPFMRTGDLGFLEAGELFVTGRLKDVIIINGRNHYPQDIEWTVEQSH
HHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEECCEEEEEECCCCCCCCCCEEEEECCC
PLIRPGCIASFSVEVGGEERLVVVAEVERKFRQLLERSQKSKIKSQKYESTDKSVGSVAV
CCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
GFFSERNGKSENKNSDNSQDLIKSIQRSVSINHDLQIYQILLLKPGAIPKTSSGKVQRYA
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHH
CREGFLAGTLKIFD
HHCCCEEEEEEECC
>Mature Secondary Structure 
SSLNKKIKTLVDLLQHRAIQNPEKIGYSFLIDGEIETVNLTYKQLEQRSRAIAAYLQSV
CHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHH
CPQGEVALLLYQPGLEYITTFFGCLYAGVIAIPAYPPRPNRSLDRIQTIIRDSKAKIALT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCEEEEE
TKSIISSLEPRASETPELENLGWLATDQIPDYFADQWLEPNINENTLAFLQYTSGSTAKP
HHHHHHHCCCCCCCCCCHHHCCCEECCCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
KGVMITHQNLLHNSGLIHQCFGHSGESKGVIWLPPYHDMGLIGGILQPLYGAFPVILMSP
CCEEEEEHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LMFLQSPVRWLKAISRYQGTTSGGPNFAYDLCVRKIKPEQIQTLDLSSWEVAFNGAEPIS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCH
AEVLERFTNTFSVCGFRREAFYPCYGMAEATLIISGGNKFSPPVETKVAARALEKNQIVL
HHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEE
VYPTEGTKTLVGCGQTLPEQQIKIVHPEKLTLCTEGEVGEIWVSGPSIAGGYWQKPEATK
EECCCCCEEEEECCCCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCHHHH
QIFAAYLAEAPGKEPFMRTGDLGFLEAGELFVTGRLKDVIIINGRNHYPQDIEWTVEQSH
HHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEECCEEEEEECCCCCCCCCCEEEEECCC
PLIRPGCIASFSVEVGGEERLVVVAEVERKFRQLLERSQKSKIKSQKYESTDKSVGSVAV
CCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
GFFSERNGKSENKNSDNSQDLIKSIQRSVSINHDLQIYQILLLKPGAIPKTSSGKVQRYA
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHH
CREGFLAGTLKIFD
HHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]