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The map label for this gene is yafV [C]

Identifier: 113475320

GI number: 113475320

Start: 2493403

End: 2494218

Strand: Reverse

Name: yafV [C]

Synonym: Tery_1632

Alternate gene names: 113475320

Gene position: 2494218-2493403 (Counterclockwise)

Preceding gene: 113475321

Following gene: 113475319

Centisome position: 32.18

GC content: 39.09

Gene sequence:

>816_bases
ATGAAATCCTATCTCGCTGCGGCTATATCAATGACAAGTGTGCCTGACTTGGCAAAAAACTTATTACAAGCAGAAGAATT
AATAGACTTAGCTATCCGTCAAGGTGCAGAATTAGTTACCTTGCCAGAAAATTTTTCTTTCTTAGGTGGAGAGGAAGAAA
AAATATCTCAAGCAGAAGCGATCGCTAAGGAGAGTGAAAAGTTTCTCAAAACAATTGCTCAAAAATATCAAATCACAATT
TTAGGAGGTGGTTATCCAGTACCGACAACAAATGGCAAAGTTTACAATGTTGCATCTTTAATTGAGCCTAATGGCATAGA
AGTAGCAAGGTATGAAAAAGCACATTTATTTGATGTTAACTTACCCGATGGTAATACTTATCAAGAATCAAATACGGTCA
AAGCAGGAATGAAGTTACCATCAGTGTATGAATCTAAAGATTATGGAAATTTAGGTATATCAGTATGCTATGATGTAAGA
TTCCCTGAACTCTATCGACATCTTTCAAATGCAGGAGCTGAAGTTTTATTTATACCAGCGGCTTTTACTGCATACACAGG
AAAAGACCATTGGCAAGTATTACTCCAAGCACGAGCTATAGAAAATACCTGTTATATTATCGCCCCGGCCCAAACAGGTA
ATCACTACGGTAGAAGACAAACTCATGGCCATGCAATGATAGTTGACCCCTGGGGTTTGATTTTGGCAGATACCGGGGAC
AAACCAGGAGTGGCGATCGCAGAAATTGACCCTACTCGCTTAGAACAAGTTCGTAGGCAAATGCCGTCCCTCCAACATAG
GGTATTTATATCTTGA

Upstream 100 bases:

>100_bases
TTGTCTATTCTTTATTTCCTATATTTCCTATTATCTAAAATCTGTTTCTTGTATATTTTACTCTAATAAAAACTTTAAGA
CTTTGTAAAAAACTGACTTA

Downstream 100 bases:

>100_bases
CGAGAAAATCAGGACTCAAATCTCCCCTTTTCCAGGTATGCATTATAAGTAACTTTTAAAACCTTTTTCTGATCACAGAA
AAAGGAAGATTATTATCTCC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKSYLAAAISMTSVPDLAKNLLQAEELIDLAIRQGAELVTLPENFSFLGGEEEKISQAEAIAKESEKFLKTIAQKYQITI
LGGGYPVPTTNGKVYNVASLIEPNGIEVARYEKAHLFDVNLPDGNTYQESNTVKAGMKLPSVYESKDYGNLGISVCYDVR
FPELYRHLSNAGAEVLFIPAAFTAYTGKDHWQVLLQARAIENTCYIIAPAQTGNHYGRRQTHGHAMIVDPWGLILADTGD
KPGVAIAEIDPTRLEQVRRQMPSLQHRVFIS

Sequences:

>Translated_271_residues
MKSYLAAAISMTSVPDLAKNLLQAEELIDLAIRQGAELVTLPENFSFLGGEEEKISQAEAIAKESEKFLKTIAQKYQITI
LGGGYPVPTTNGKVYNVASLIEPNGIEVARYEKAHLFDVNLPDGNTYQESNTVKAGMKLPSVYESKDYGNLGISVCYDVR
FPELYRHLSNAGAEVLFIPAAFTAYTGKDHWQVLLQARAIENTCYIIAPAQTGNHYGRRQTHGHAMIVDPWGLILADTGD
KPGVAIAEIDPTRLEQVRRQMPSLQHRVFIS
>Mature_271_residues
MKSYLAAAISMTSVPDLAKNLLQAEELIDLAIRQGAELVTLPENFSFLGGEEEKISQAEAIAKESEKFLKTIAQKYQITI
LGGGYPVPTTNGKVYNVASLIEPNGIEVARYEKAHLFDVNLPDGNTYQESNTVKAGMKLPSVYESKDYGNLGISVCYDVR
FPELYRHLSNAGAEVLFIPAAFTAYTGKDHWQVLLQARAIENTCYIIAPAQTGNHYGRRQTHGHAMIVDPWGLILADTGD
KPGVAIAEIDPTRLEQVRRQMPSLQHRVFIS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=39.9253731343284, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=39.9253731343284, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=39.9253731343284, Blast_Score=200, Evalue=9e-52,
Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=32.5842696629214, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=35.7512953367876, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786412, Length=232, Percent_Identity=27.1551724137931, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556280, Length=268, Percent_Identity=39.9253731343284, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322335, Length=297, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=265, Percent_Identity=33.2075471698113, Blast_Score=162, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17933642, Length=259, Percent_Identity=38.2239382239382, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21355835, Length=263, Percent_Identity=31.5589353612167, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29911; Mature: 29911

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYLAAAISMTSVPDLAKNLLQAEELIDLAIRQGAELVTLPENFSFLGGEEEKISQAEA
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHH
IAKESEKFLKTIAQKYQITILGGGYPVPTTNGKVYNVASLIEPNGIEVARYEKAHLFDVN
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEHHCCCCCCEEEEECCEEEEEEE
LPDGNTYQESNTVKAGMKLPSVYESKDYGNLGISVCYDVRFPELYRHLSNAGAEVLFIPA
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEC
AFTAYTGKDHWQVLLQARAIENTCYIIAPAQTGNHYGRRQTHGHAMIVDPWGLILADTGD
EEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCEEEEECCCC
KPGVAIAEIDPTRLEQVRRQMPSLQHRVFIS
CCCEEEEECCHHHHHHHHHHCCCHHHEEECC
>Mature Secondary Structure
MKSYLAAAISMTSVPDLAKNLLQAEELIDLAIRQGAELVTLPENFSFLGGEEEKISQAEA
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHH
IAKESEKFLKTIAQKYQITILGGGYPVPTTNGKVYNVASLIEPNGIEVARYEKAHLFDVN
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEHHCCCCCCEEEEECCEEEEEEE
LPDGNTYQESNTVKAGMKLPSVYESKDYGNLGISVCYDVRFPELYRHLSNAGAEVLFIPA
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEC
AFTAYTGKDHWQVLLQARAIENTCYIIAPAQTGNHYGRRQTHGHAMIVDPWGLILADTGD
EEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCEEEEECCCC
KPGVAIAEIDPTRLEQVRRQMPSLQHRVFIS
CCCEEEEECCHHHHHHHHHHCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]