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The map label for this gene is pnp

Identifier: 113475234

GI number: 113475234

Start: 2339841

End: 2341994

Strand: Reverse

Name: pnp

Synonym: Tery_1541

Alternate gene names: 113475234

Gene position: 2341994-2339841 (Counterclockwise)

Preceding gene: 113475242

Following gene: 113475229

Centisome position: 30.22

GC content: 41.5

Gene sequence:

>2154_bases
ATGGAAGAGATAGATAAATCCATATCCTTTGACGGACGGGATATTCGACTCAAAGTTGGTTTATTTGCACCTCAAGCTGG
TGGTGCTGTGATGATAGAGTCAGGAGACACAGCAGTATTAGTCACAGCTACTACAGCTAAAGGTAGAGAAGGAATAGATT
TTCTACCTCTTTTGGTTGACTATGAGGAAAGACTCTACGCTGGAGGAAAAATTCCCGGTGGTTTTTTGCGCCGGGAAGGT
CGTCCTCCAGAAAAGGTGACACTGACAAGTCGTTTAATTGATAGACCTCTACGCCCCCTATTTCCTAGTTGGTTACGGGA
TGATATTCAAATTGTGGCCACAACATTGTCCATGGATGAGGAAGTACCACCAGATGTGTTAGCCGTAACAGGTAGTTCAG
TAGCAGTATTACTGGCCAAATTACCTTTTTATGGCCCAATGGCTGCAGTACGGGTAGGGCTAGTAGGGGATGACTTTATC
ATTAATCCTACTTACCGTGAAGTCAAAAATGGTGATTTAGACTTAGTGGTCGCTGGGTCTCCCCAGGGGGTGATCATGGT
AGAAGCAGGGGCAAATCAATTGCCAGAACAAGATATTATTGAGGCAATTGATTTTGGCTATGAAGCAGTTTGTGACCTGA
TTCAAGCTCAACGAGAAATTATTGCTGAAATGGGTATTGAACTTGTAGAATTAGAACCTCCGGAAGTAGACCCTACCTTA
GAAAACTATATCAAGGAAAAAGCTACAGAACAGATTAAAGAGATTTTGTCTCAATATGATTTAGATAAAAATCAGCGGGA
CGAAAAACTCGATGAAATTAAAGATTCTCTGGCAGAAAGCATCACTGAACTTCCTGAAGAAGAAGCAGTAAGAGCAATGG
TTGAATCTGAAAGTATGTTGCTTGGGAATGCCTTTAAGAGCGTCACCAAAAAGTTAATGCGTCAACAAATTGTTGCTGAA
GGTATCCGGGTTGATGGTCGGAAGTTAGATGAAGTACGCCCTGTTTCTTGTCGGGTAGGAGTACTGCCATCACGAGTCCA
CGGTAGTTCTCTATTTAATCGAGGTTTAACTCAAGTAATGTCTGCTGTTACTTTGGGAACACCAGGTGATGCTCAAGAAC
TAGCGGATGATTTACATCCCCAAGACGAAAAACGTTATTTGCACCATTACAACTTCCCTCCTTTTTCTGTAGGAGAAACC
AAACCATTGCGACAGCCTGGTCGTAGAGAAATTGGTCATGGTGCTTTAGCAGAACGTGCTTTGATACCTGTCATACCAAA
AAGCGATATTTTTCCCTATGTAATTCGAGTCGTTTCAGAAGTCCTTTCTTCAAATGGTTCAACTTCTATGGGTTCAGTTT
GTGCTTCTACTCTTGGTTTAATGGATGCTGGAGTTCCTATTGTTAAACCCGTGAGTGGAGCAGCTATGGGTTTAATCAAA
GAAGATGAGGAAGTCCGAATTCTGACAGATATTCAGGGTATAGAAGATTTTTTGGGTGATATGGACTTCAAAGTTGCTGG
TACCGATAGTGGAATTACAGCTTTACAGATGGATATGAAAATTACAGGTTTGCCTATAGAGGTCATTGCTGATGCTATTC
ATCAAGCTAAACCAGCACGGATACATATTTTAGACAAGATGCTAAGTGTTCTCGATCTACCTCGTCCCGATATGTCGCCC
TATGCACCGAGACTCTTAAGCTTCAAGATAGACCCAGAAATGATCGGTTTAGTCATCGGACCTGGTGGTAAAACTATTAA
AGGTATTACTGAGGAAACCGGTGTTAAAATTGATATTGATGATGATGGAACCGTAACTATTGCAGCAGCAGATGGTGAAA
AAGCTAAACAAGCTTGCAATATTATTCAAGGGATGACCAAGAAACTCAATCCTGGAGATGTTTATGTTGGTCGAGTGACT
CGAATTATTCCTATTGGTGCTTTTGTAGAAGTATTTGCTGGAAAAGAAGGAATGGTTCATATATCCCAAATTGCAGATTA
TCGCGTAGGCAAGGTAGAAGATGAGCTAGCAATTGGAGATGAGGTCATTGTTAAAGTACGAGAAATAGATTCTAAGGGCC
GAGTGAATTTAACTCGCTTGAATATTCACCCTGACGAAGCTGCCGCAGCTCGTGCTAATGCAATGAATTATTAA

Upstream 100 bases:

>100_bases
TTCTGACCTCAGAGTTCCAACTTAAGCAATATAACACTTGCCCCATCAACAATGCTAGGATTGCCACAGAAACAAAAGAA
TATAGAAGGAAAAAAACTGG

Downstream 100 bases:

>100_bases
AGTATAGTTACAAGTCAAAAGTCAGACGTCAGAAATGGGGAGCAAAAATTCTGTTTTTATAGAAACCATTTAAAACCTCC
TTACCTAACCATCAACCTAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MEEIDKSISFDGRDIRLKVGLFAPQAGGAVMIESGDTAVLVTATTAKGREGIDFLPLLVDYEERLYAGGKIPGGFLRREG
RPPEKVTLTSRLIDRPLRPLFPSWLRDDIQIVATTLSMDEEVPPDVLAVTGSSVAVLLAKLPFYGPMAAVRVGLVGDDFI
INPTYREVKNGDLDLVVAGSPQGVIMVEAGANQLPEQDIIEAIDFGYEAVCDLIQAQREIIAEMGIELVELEPPEVDPTL
ENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESMLLGNAFKSVTKKLMRQQIVAE
GIRVDGRKLDEVRPVSCRVGVLPSRVHGSSLFNRGLTQVMSAVTLGTPGDAQELADDLHPQDEKRYLHHYNFPPFSVGET
KPLRQPGRREIGHGALAERALIPVIPKSDIFPYVIRVVSEVLSSNGSTSMGSVCASTLGLMDAGVPIVKPVSGAAMGLIK
EDEEVRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKITGLPIEVIADAIHQAKPARIHILDKMLSVLDLPRPDMSP
YAPRLLSFKIDPEMIGLVIGPGGKTIKGITEETGVKIDIDDDGTVTIAAADGEKAKQACNIIQGMTKKLNPGDVYVGRVT
RIIPIGAFVEVFAGKEGMVHISQIADYRVGKVEDELAIGDEVIVKVREIDSKGRVNLTRLNIHPDEAAAARANAMNY

Sequences:

>Translated_717_residues
MEEIDKSISFDGRDIRLKVGLFAPQAGGAVMIESGDTAVLVTATTAKGREGIDFLPLLVDYEERLYAGGKIPGGFLRREG
RPPEKVTLTSRLIDRPLRPLFPSWLRDDIQIVATTLSMDEEVPPDVLAVTGSSVAVLLAKLPFYGPMAAVRVGLVGDDFI
INPTYREVKNGDLDLVVAGSPQGVIMVEAGANQLPEQDIIEAIDFGYEAVCDLIQAQREIIAEMGIELVELEPPEVDPTL
ENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESMLLGNAFKSVTKKLMRQQIVAE
GIRVDGRKLDEVRPVSCRVGVLPSRVHGSSLFNRGLTQVMSAVTLGTPGDAQELADDLHPQDEKRYLHHYNFPPFSVGET
KPLRQPGRREIGHGALAERALIPVIPKSDIFPYVIRVVSEVLSSNGSTSMGSVCASTLGLMDAGVPIVKPVSGAAMGLIK
EDEEVRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKITGLPIEVIADAIHQAKPARIHILDKMLSVLDLPRPDMSP
YAPRLLSFKIDPEMIGLVIGPGGKTIKGITEETGVKIDIDDDGTVTIAAADGEKAKQACNIIQGMTKKLNPGDVYVGRVT
RIIPIGAFVEVFAGKEGMVHISQIADYRVGKVEDELAIGDEVIVKVREIDSKGRVNLTRLNIHPDEAAAARANAMNY
>Mature_717_residues
MEEIDKSISFDGRDIRLKVGLFAPQAGGAVMIESGDTAVLVTATTAKGREGIDFLPLLVDYEERLYAGGKIPGGFLRREG
RPPEKVTLTSRLIDRPLRPLFPSWLRDDIQIVATTLSMDEEVPPDVLAVTGSSVAVLLAKLPFYGPMAAVRVGLVGDDFI
INPTYREVKNGDLDLVVAGSPQGVIMVEAGANQLPEQDIIEAIDFGYEAVCDLIQAQREIIAEMGIELVELEPPEVDPTL
ENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESMLLGNAFKSVTKKLMRQQIVAE
GIRVDGRKLDEVRPVSCRVGVLPSRVHGSSLFNRGLTQVMSAVTLGTPGDAQELADDLHPQDEKRYLHHYNFPPFSVGET
KPLRQPGRREIGHGALAERALIPVIPKSDIFPYVIRVVSEVLSSNGSTSMGSVCASTLGLMDAGVPIVKPVSGAAMGLIK
EDEEVRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKITGLPIEVIADAIHQAKPARIHILDKMLSVLDLPRPDMSP
YAPRLLSFKIDPEMIGLVIGPGGKTIKGITEETGVKIDIDDDGTVTIAAADGEKAKQACNIIQGMTKKLNPGDVYVGRVT
RIIPIGAFVEVFAGKEGMVHISQIADYRVGKVEDELAIGDEVIVKVREIDSKGRVNLTRLNIHPDEAAAARANAMNY

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=36.6433566433566, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=47.7011494252874, Blast_Score=618, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI115534063, Length=648, Percent_Identity=35.3395061728395, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=36.9627507163324, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=36.9627507163324, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=36.9627507163324, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=36.4485981308411, Blast_Score=400, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_TRIEI (Q115K2)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_721295.1
- ProteinModelPortal:   Q115K2
- STRING:   Q115K2
- GeneID:   4242020
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_1541
- NMPDR:   fig|203124.1.peg.5636
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q115K2
- ProtClustDB:   PRK11824
- BioCyc:   TERY203124:TERY_1541-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77948; Mature: 77948

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIDKSISFDGRDIRLKVGLFAPQAGGAVMIESGDTAVLVTATTAKGREGIDFLPLLVD
CCCHHCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCHHHHHHC
YEERLYAGGKIPGGFLRREGRPPEKVTLTSRLIDRPLRPLFPSWLRDDIQIVATTLSMDE
HHHHHHCCCCCCCHHHHCCCCCCCCEEHHHHHHHCCCHHCCHHHHHCCHHEEHHHHCCCC
EVPPDVLAVTGSSVAVLLAKLPFYGPMAAVRVGLVGDDFIINPTYREVKNGDLDLVVAGS
CCCCCEEEECCCHHHHHHHHCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PQGVIMVEAGANQLPEQDIIEAIDFGYEAVCDLIQAQREIIAEMGIELVELEPPEVDPTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
ENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESML
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
LGNAFKSVTKKLMRQQIVAEGIRVDGRKLDEVRPVSCRVGVLPSRVHGSSLFNRGLTQVM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
SAVTLGTPGDAQELADDLHPQDEKRYLHHYNFPPFSVGETKPLRQPGRREIGHGALAERA
HHHHCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCC
LIPVIPKSDIFPYVIRVVSEVLSSNGSTSMGSVCASTLGLMDAGVPIVKPVSGAAMGLIK
EEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEC
EDEEVRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKITGLPIEVIADAIHQAKPAR
CCCCEEEEHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCH
IHILDKMLSVLDLPRPDMSPYAPRLLSFKIDPEMIGLVIGPGGKTIKGITEETGVKIDID
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCEECCCHHHCCEEEEEC
DDGTVTIAAADGEKAKQACNIIQGMTKKLNPGDVYVGRVTRIIPIGAFVEVFAGKEGMVH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECHHEEEEHHHHHHHHCCCCCCEE
ISQIADYRVGKVEDELAIGDEVIVKVREIDSKGRVNLTRLNIHPDEAAAARANAMNY
HHHHHCCCCCCCCHHHHCCHHHEEEEEECCCCCCEEEEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MEEIDKSISFDGRDIRLKVGLFAPQAGGAVMIESGDTAVLVTATTAKGREGIDFLPLLVD
CCCHHCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCHHHHHHC
YEERLYAGGKIPGGFLRREGRPPEKVTLTSRLIDRPLRPLFPSWLRDDIQIVATTLSMDE
HHHHHHCCCCCCCHHHHCCCCCCCCEEHHHHHHHCCCHHCCHHHHHCCHHEEHHHHCCCC
EVPPDVLAVTGSSVAVLLAKLPFYGPMAAVRVGLVGDDFIINPTYREVKNGDLDLVVAGS
CCCCCEEEECCCHHHHHHHHCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PQGVIMVEAGANQLPEQDIIEAIDFGYEAVCDLIQAQREIIAEMGIELVELEPPEVDPTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
ENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESML
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
LGNAFKSVTKKLMRQQIVAEGIRVDGRKLDEVRPVSCRVGVLPSRVHGSSLFNRGLTQVM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
SAVTLGTPGDAQELADDLHPQDEKRYLHHYNFPPFSVGETKPLRQPGRREIGHGALAERA
HHHHCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCC
LIPVIPKSDIFPYVIRVVSEVLSSNGSTSMGSVCASTLGLMDAGVPIVKPVSGAAMGLIK
EEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEC
EDEEVRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKITGLPIEVIADAIHQAKPAR
CCCCEEEEHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCH
IHILDKMLSVLDLPRPDMSPYAPRLLSFKIDPEMIGLVIGPGGKTIKGITEETGVKIDID
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCEECCCHHHCCEEEEEC
DDGTVTIAAADGEKAKQACNIIQGMTKKLNPGDVYVGRVTRIIPIGAFVEVFAGKEGMVH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECHHEEEEHHHHHHHHCCCCCCEE
ISQIADYRVGKVEDELAIGDEVIVKVREIDSKGRVNLTRLNIHPDEAAAARANAMNY
HHHHHCCCCCCCCHHHHCCHHHEEEEEECCCCCCEEEEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA