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The map label for this gene is gatA2 [H]

Identifier: 113475075

GI number: 113475075

Start: 2060638

End: 2061978

Strand: Reverse

Name: gatA2 [H]

Synonym: Tery_1350

Alternate gene names: 113475075

Gene position: 2061978-2060638 (Counterclockwise)

Preceding gene: 113475076

Following gene: 113475074

Centisome position: 26.61

GC content: 34.23

Gene sequence:

>1341_bases
GTGAAATTAACATCTTTATCTGCCCATACCTTAGCAAAAATAATTCGTGAACGCCTGGTTTCTTGTGAAGAAGTAGTGAC
AGCTTATCTTCAACGTATTTCTTACTATAACCCTCAATTAAATGCTATTATTACTCTCGATCCAGAACAAGTTGATCAAC
AGGTTAAAAAAGCAGATCGTGCCTTAGCAAAAGGAAAATGTTTTGGACCTTTACACGGAGTTCCTATTACTATTAAAGAC
AGTTTAGAAACGAAAGGGCTGAGAACAACTTGTAGTTATGAACCTTTAGCTAACTATATTCCCCGAAAAGATGCAACGGT
TGTAGCAAAATTAAAAGCAGCCGGCGCTATTATTTTAGGTAAGACTAATACCCCAAAATTAACCGGAGATTTTCAAACCA
ATAGTCCTTTATTTGGTCGCACAAATAACCCTTGGAATTTAGACTATACACCAGGGGGAAGTACAGGAGGAGGTGGGTCT
GCGATCGCTGCTCAATTATCGGTTTTTGACATAGGAAGTGATTTGGGAGGGTCGCTTCGAATTCCGGCTCATTTTTGTGG
TATTTATACCATTAAAGCAACAGAAAAGAGGGTTTCAACCTATGGTCATATTCCTGAATTACCGGGTAATCCTTTAACAA
TTAATCATTGTCAAAATGTCGGATATTTGGGACGATCAGTTGAAGATTTAATGTTATGTTTTTCGGTCATAAAAGAAAGT
AATCATCAAGGATTAAATGAAAAAATTAACTCATTTTCTAGTTTAAATTCTTATGGCTTTGTATGGTCTTATGGTTTCGG
TAATATTCCTTGTTGTTCTGAAACTAAAAAAGCTATTAAAACAGTTATTGATGTTTTATCTGAGTTAGATTATCAGCTAG
AATATAGAAATCTTTCTCAAATCAATATGGATGAAGTTTGGGAGATTTATAGAAATATTTTAAGCTATGAATTTAATCCT
TCAACGCCAAAAGATGAGCAGAAAAACTATATGATTGCTCTTGAAAAACGTCAAATGATTATGAATCAATTAGCCCTATT
TTTTCAGAAGGTTGATATTTGGTTAATTCCAGTTGTTCCTTTTCCTGCTTTTCCTCGTTTTCCTTTTGGTAATGATATAG
ATATAGAGGGAAAAAGTTATCCATATTTTCAAGCGATCGGTGCTTATACAACCTTATTTAATTTAACTGGTTATCCTGTG
GTTGTTATCCCATTAACTTTATCCTCTAAGGGGCTCCCTATTGGTGTTCAATTAGTAGGAAACTATGGAAATGATCAAAA
GCTTTTAACAATTGCTAACCAGATCAGTAGCATTATTAATCCTTTACCTTGTCCTTTATAA

Upstream 100 bases:

>100_bases
AATGACTAAAAATTAGAATTGTATTGGCATTCAAATCTATAGTTTTTAATCCCCTATTTTATTTTACAACTGATAGACAT
ACCAATGATGATTAATTAAA

Downstream 100 bases:

>100_bases
AATTGAAAATAAATTATAGATATAGCTTTTATATTTATTTAAGTGATAAATACTATTATTTATATAAATATATAACTGAT
GAGATTAAATAAATCCCAAA

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKLTSLSAHTLAKIIRERLVSCEEVVTAYLQRISYYNPQLNAIITLDPEQVDQQVKKADRALAKGKCFGPLHGVPITIKD
SLETKGLRTTCSYEPLANYIPRKDATVVAKLKAAGAIILGKTNTPKLTGDFQTNSPLFGRTNNPWNLDYTPGGSTGGGGS
AIAAQLSVFDIGSDLGGSLRIPAHFCGIYTIKATEKRVSTYGHIPELPGNPLTINHCQNVGYLGRSVEDLMLCFSVIKES
NHQGLNEKINSFSSLNSYGFVWSYGFGNIPCCSETKKAIKTVIDVLSELDYQLEYRNLSQINMDEVWEIYRNILSYEFNP
STPKDEQKNYMIALEKRQMIMNQLALFFQKVDIWLIPVVPFPAFPRFPFGNDIDIEGKSYPYFQAIGAYTTLFNLTGYPV
VVIPLTLSSKGLPIGVQLVGNYGNDQKLLTIANQISSIINPLPCPL

Sequences:

>Translated_446_residues
MKLTSLSAHTLAKIIRERLVSCEEVVTAYLQRISYYNPQLNAIITLDPEQVDQQVKKADRALAKGKCFGPLHGVPITIKD
SLETKGLRTTCSYEPLANYIPRKDATVVAKLKAAGAIILGKTNTPKLTGDFQTNSPLFGRTNNPWNLDYTPGGSTGGGGS
AIAAQLSVFDIGSDLGGSLRIPAHFCGIYTIKATEKRVSTYGHIPELPGNPLTINHCQNVGYLGRSVEDLMLCFSVIKES
NHQGLNEKINSFSSLNSYGFVWSYGFGNIPCCSETKKAIKTVIDVLSELDYQLEYRNLSQINMDEVWEIYRNILSYEFNP
STPKDEQKNYMIALEKRQMIMNQLALFFQKVDIWLIPVVPFPAFPRFPFGNDIDIEGKSYPYFQAIGAYTTLFNLTGYPV
VVIPLTLSSKGLPIGVQLVGNYGNDQKLLTIANQISSIINPLPCPL
>Mature_446_residues
MKLTSLSAHTLAKIIRERLVSCEEVVTAYLQRISYYNPQLNAIITLDPEQVDQQVKKADRALAKGKCFGPLHGVPITIKD
SLETKGLRTTCSYEPLANYIPRKDATVVAKLKAAGAIILGKTNTPKLTGDFQTNSPLFGRTNNPWNLDYTPGGSTGGGGS
AIAAQLSVFDIGSDLGGSLRIPAHFCGIYTIKATEKRVSTYGHIPELPGNPLTINHCQNVGYLGRSVEDLMLCFSVIKES
NHQGLNEKINSFSSLNSYGFVWSYGFGNIPCCSETKKAIKTVIDVLSELDYQLEYRNLSQINMDEVWEIYRNILSYEFNP
STPKDEQKNYMIALEKRQMIMNQLALFFQKVDIWLIPVVPFPAFPRFPFGNDIDIEGKSYPYFQAIGAYTTLFNLTGYPV
VVIPLTLSSKGLPIGVQLVGNYGNDQKLLTIANQISSIINPLPCPL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=29.2433537832311, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI222831590, Length=468, Percent_Identity=27.991452991453, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI166795287, Length=189, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=31.6205533596838, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=307, Percent_Identity=29.6416938110749, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=210, Percent_Identity=33.8095238095238, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=146, Percent_Identity=34.2465753424658, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=155, Percent_Identity=38.0645161290323, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=174, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=174, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=232, Percent_Identity=29.3103448275862, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=400, Percent_Identity=26.25, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=201, Percent_Identity=30.3482587064677, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=178, Percent_Identity=31.4606741573034, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648435, Length=481, Percent_Identity=27.6507276507277, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24648437, Length=481, Percent_Identity=27.6507276507277, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45550774, Length=483, Percent_Identity=27.9503105590062, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21356731, Length=483, Percent_Identity=28.7784679089027, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=25.7731958762887, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=25.7731958762887, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=25.7731958762887, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=25.7731958762887, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=27.7665995975855, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=24.1935483870968, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648441, Length=407, Percent_Identity=26.7813267813268, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648439, Length=407, Percent_Identity=26.7813267813268, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648113, Length=204, Percent_Identity=40.1960784313725, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 49355; Mature: 49355

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTSLSAHTLAKIIRERLVSCEEVVTAYLQRISYYNPQLNAIITLDPEQVDQQVKKADR
CCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHH
ALAKGKCFGPLHGVPITIKDSLETKGLRTTCSYEPLANYIPRKDATVVAKLKAAGAIILG
HHHCCCCCCCCCCCCEEEECCCCCCCCCEECCCCHHHHHCCCCCCCEEEEEHHCCEEEEE
KTNTPKLTGDFQTNSPLFGRTNNPWNLDYTPGGSTGGGGSAIAAQLSVFDIGSDLGGSLR
CCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHEEEEEEEECCCCCCCCEE
IPAHFCGIYTIKATEKRVSTYGHIPELPGNPLTINHCQNVGYLGRSVEDLMLCFSVIKES
CCEEEEEEEEEECHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
NHQGLNEKINSFSSLNSYGFVWSYGFGNIPCCSETKKAIKTVIDVLSELDYQLEYRNLSQ
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
INMDEVWEIYRNILSYEFNPSTPKDEQKNYMIALEKRQMIMNQLALFFQKVDIWLIPVVP
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEECC
FPAFPRFPFGNDIDIEGKSYPYFQAIGAYTTLFNLTGYPVVVIPLTLSSKGLPIGVQLVG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEE
NYGNDQKLLTIANQISSIINPLPCPL
CCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLTSLSAHTLAKIIRERLVSCEEVVTAYLQRISYYNPQLNAIITLDPEQVDQQVKKADR
CCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHH
ALAKGKCFGPLHGVPITIKDSLETKGLRTTCSYEPLANYIPRKDATVVAKLKAAGAIILG
HHHCCCCCCCCCCCCEEEECCCCCCCCCEECCCCHHHHHCCCCCCCEEEEEHHCCEEEEE
KTNTPKLTGDFQTNSPLFGRTNNPWNLDYTPGGSTGGGGSAIAAQLSVFDIGSDLGGSLR
CCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHEEEEEEEECCCCCCCCEE
IPAHFCGIYTIKATEKRVSTYGHIPELPGNPLTINHCQNVGYLGRSVEDLMLCFSVIKES
CCEEEEEEEEEECHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
NHQGLNEKINSFSSLNSYGFVWSYGFGNIPCCSETKKAIKTVIDVLSELDYQLEYRNLSQ
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
INMDEVWEIYRNILSYEFNPSTPKDEQKNYMIALEKRQMIMNQLALFFQKVDIWLIPVVP
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEECC
FPAFPRFPFGNDIDIEGKSYPYFQAIGAYTTLFNLTGYPVVVIPLTLSSKGLPIGVQLVG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEE
NYGNDQKLLTIANQISSIINPLPCPL
CCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]