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The map label for this gene is spsK [H]

Identifier: 113475071

GI number: 113475071

Start: 2049484

End: 2050365

Strand: Reverse

Name: spsK [H]

Synonym: Tery_1344

Alternate gene names: 113475071

Gene position: 2050365-2049484 (Counterclockwise)

Preceding gene: 113475072

Following gene: 113475070

Centisome position: 26.46

GC content: 39.57

Gene sequence:

>882_bases
ATGAAAATCTTATTAACAGGTATTAACGGACAACTAGGAAACCAGCTACAAACACCTCTCAAAAAAATAGGAGAAGTATC
AGTAGTGGGGCGAGAAAACTTAGACCTAGCAGACTCCAATGCTATCTCCCAACTTATAGGCCAAGTTCAACCAGAACTAA
TTATCAATGCTGCAGCTTATACAGCAGTTGACAAATCTGAAAAGGAACCAGAATTAGCTGATGCTGTCAATAGGATCGCT
CCTGGAATTATGGCAAAAGAGGCAAAAAAGTTGGGGGCAACCTTAATACACATTTCCACCGACTATGTTTTTGATGGTAG
TCAAAGCAGGCCCTACACAGAAACCGACTTGACTAACCCCCTCGGAATTTATGGTAAATCTAAGTTAGCTGGAGAAGAGG
CAATTCGAGAAACAGAAGTTAATCATATTATTATCAGAACAGCTTGGGTCTATGGAGCCTATGGAAAAGGTAACTTTGTC
AAAACTATGTTGCGCTTAGGTAAGGATAGAGAAGAATTAAGGGTTGTCTATGATCAGGTCGGTTGCCCAACTTGGACTGG
AGACTTAGCAGAGGCGATCGCTCAAATTATTCCTCATCTTAATTCCCAAACTTATGGAACTTATCACTATACAAATAGTG
GAGCTATTAGTTGGTATGATTTTGCTATGGCTATTTTTGAGGAAGCAAAAAAACTGGGTTTTCCTTTAGAGGTAAAACGA
GTAGTTCCCATTACTACTGCCGAGTATCCAACTCCAGCAAAACGACCTGCCTTTTCAGTTTTGAATAGTGGCAAAATTTC
AAAAGTTTTGGGAAACCATCCTCCATACTGGTTAAATAGTTTGAGGCAAATGTTAAAGCAGTTAGCTGCTGTAACCAATT
AA

Upstream 100 bases:

>100_bases
GAATAGGGAGTTAAAACTCTAAGCTAAAAATCATTTTTTTCAAATCTAATCAAAATAAAATCGAAAAATTTAACTGAAAC
TTAGTCACGAAAAATTACCA

Downstream 100 bases:

>100_bases
CAATTTGACAGCTAGAATTTTTGTGATTTAAATTTGGAGCTAATGAATACTATTGCTACTCCGTCTCCTTGGATAAAAAT
CTTTCAACCTTGTCCTCAAC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKILLTGINGQLGNQLQTPLKKIGEVSVVGRENLDLADSNAISQLIGQVQPELIINAAAYTAVDKSEKEPELADAVNRIA
PGIMAKEAKKLGATLIHISTDYVFDGSQSRPYTETDLTNPLGIYGKSKLAGEEAIRETEVNHIIIRTAWVYGAYGKGNFV
KTMLRLGKDREELRVVYDQVGCPTWTGDLAEAIAQIIPHLNSQTYGTYHYTNSGAISWYDFAMAIFEEAKKLGFPLEVKR
VVPITTAEYPTPAKRPAFSVLNSGKISKVLGNHPPYWLNSLRQMLKQLAAVTN

Sequences:

>Translated_293_residues
MKILLTGINGQLGNQLQTPLKKIGEVSVVGRENLDLADSNAISQLIGQVQPELIINAAAYTAVDKSEKEPELADAVNRIA
PGIMAKEAKKLGATLIHISTDYVFDGSQSRPYTETDLTNPLGIYGKSKLAGEEAIRETEVNHIIIRTAWVYGAYGKGNFV
KTMLRLGKDREELRVVYDQVGCPTWTGDLAEAIAQIIPHLNSQTYGTYHYTNSGAISWYDFAMAIFEEAKKLGFPLEVKR
VVPITTAEYPTPAKRPAFSVLNSGKISKVLGNHPPYWLNSLRQMLKQLAAVTN
>Mature_293_residues
MKILLTGINGQLGNQLQTPLKKIGEVSVVGRENLDLADSNAISQLIGQVQPELIINAAAYTAVDKSEKEPELADAVNRIA
PGIMAKEAKKLGATLIHISTDYVFDGSQSRPYTETDLTNPLGIYGKSKLAGEEAIRETEVNHIIIRTAWVYGAYGKGNFV
KTMLRLGKDREELRVVYDQVGCPTWTGDLAEAIAQIIPHLNSQTYGTYHYTNSGAISWYDFAMAIFEEAKKLGFPLEVKR
VVPITTAEYPTPAKRPAFSVLNSGKISKVLGNHPPYWLNSLRQMLKQLAAVTN

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=289, Percent_Identity=31.1418685121107, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI33519455, Length=289, Percent_Identity=31.1418685121107, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=43.4782608695652, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32204; Mature: 32204

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLTGINGQLGNQLQTPLKKIGEVSVVGRENLDLADSNAISQLIGQVQPELIINAAAY
CEEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHCCHHHEEEHHHH
TAVDKSEKEPELADAVNRIAPGIMAKEAKKLGATLIHISTDYVFDGSQSRPYTETDLTNP
HHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCCCCCC
LGIYGKSKLAGEEAIRETEVNHIIIRTAWVYGAYGKGNFVKTMLRLGKDREELRVVYDQV
CCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHC
GCPTWTGDLAEAIAQIIPHLNSQTYGTYHYTNSGAISWYDFAMAIFEEAKKLGFPLEVKR
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
VVPITTAEYPTPAKRPAFSVLNSGKISKVLGNHPPYWLNSLRQMLKQLAAVTN
EEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILLTGINGQLGNQLQTPLKKIGEVSVVGRENLDLADSNAISQLIGQVQPELIINAAAY
CEEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHCCHHHEEEHHHH
TAVDKSEKEPELADAVNRIAPGIMAKEAKKLGATLIHISTDYVFDGSQSRPYTETDLTNP
HHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCCCCCC
LGIYGKSKLAGEEAIRETEVNHIIIRTAWVYGAYGKGNFVKTMLRLGKDREELRVVYDQV
CCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHC
GCPTWTGDLAEAIAQIIPHLNSQTYGTYHYTNSGAISWYDFAMAIFEEAKKLGFPLEVKR
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
VVPITTAEYPTPAKRPAFSVLNSGKISKVLGNHPPYWLNSLRQMLKQLAAVTN
EEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]