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The map label for this gene is rpoD2 [H]

Identifier: 113474616

GI number: 113474616

Start: 1251949

End: 1252959

Strand: Direct

Name: rpoD2 [H]

Synonym: Tery_0784

Alternate gene names: 113474616

Gene position: 1251949-1252959 (Clockwise)

Preceding gene: 113474615

Following gene: 113474617

Centisome position: 16.15

GC content: 38.18

Gene sequence:

>1011_bases
ATGTCAAAGCTAAATCAAGAAAATAAAAATCAAAAGAAAACTCAAACTAAATTTGGTCCTACTACTTACTCTTCAATGGA
CACAGTTCGTACCTACTTACATGAGATTGGTCGTGTACCACTTTTAACTCGTGAGGAAGAGATTGTTTATGGTAAACAAG
TGCAGCAAATGATGCAATTCCTAGAATTAAAAGAAAAATTAGAGGAAAAACTTAATAGAACAGCAACTTTACTAGAATGG
GCAGAACATTTACTAGTAAGTGAAAAAGTACTTAAGCAAGCAATTAAGCAAGGTCAACGTGCCAAGCAAAAAATGATTGA
AGCTAATCTTCGCTTAGTAGTAGCGATCGCTAAAAAGTATCAAAAGCGAAATATGGAGTTCTTAGATTTAATTCAAGAAG
GTACTCTTGGTTTAGAAAGAGGGGTGGAAAAATTTGATCCTACACGGGGATACAAATTTTCTACTTATGCTTACTGGTGG
ATTCGTCAGGCAATTACTAGAGCGATCGCACAACAAGCTCGTACTATTCGCCTACCTATTCATATTACTGAAAAGCTCAA
CAAAATCAAAAGAGTTCAGAGAGAACTAATTCAGAGTTTGGGTCGTAGTCCTACTCCTTCTGAGATTGCCCAAGCTTTAG
AATTAGAACCTTCTCAGATTAGAGAATATCTTTTGATGGCCCGTCATCCTATTTCCTTAGATTTAAGAGTTGGAGATAAT
CAGGATACTGAACTGCAAGAACTTTTGGAGGATGAAACATCTTCTCCGGATGACTACATAACTGGTGAGTTATTGCGTCA
AGATATAAATACCTTACTGGCAGAATTGAGTGAGCAACAACGTCGTGTATTGGTATTGCGTTTTGGTCTTGAGGATGGTA
AGGAGATGTCTTTAGCAAAAGTGGGAGATAAACTTCAACTTAGTCGTGAGCGAGTTCGCCAACTAGAACATCAAGCTTTG
GCCTTTTTACGCCGTCGTCAGGCCAAGGTGCGGGAGTATGTAGCTAGCTAA

Upstream 100 bases:

>100_bases
TAGACAAGGATATCCTTATCTAGTTGGATATCTATGTATTTCTAACCTAAGAAGAAAATTATAACTACTGCAATATTTAT
TAAAGGAGTTTATTAAAAAA

Downstream 100 bases:

>100_bases
CTGAAGTCAGAAGCCAACTTCCCCTTAGCTTCTCTAAGGGGAGAAATCAGTGGAAACATATATATATTCCACGCTACATT
TCCTAACTGCTATAGTATTT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSKLNQENKNQKKTQTKFGPTTYSSMDTVRTYLHEIGRVPLLTREEEIVYGKQVQQMMQFLELKEKLEEKLNRTATLLEW
AEHLLVSEKVLKQAIKQGQRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPTRGYKFSTYAYWW
IRQAITRAIAQQARTIRLPIHITEKLNKIKRVQRELIQSLGRSPTPSEIAQALELEPSQIREYLLMARHPISLDLRVGDN
QDTELQELLEDETSSPDDYITGELLRQDINTLLAELSEQQRRVLVLRFGLEDGKEMSLAKVGDKLQLSRERVRQLEHQAL
AFLRRRQAKVREYVAS

Sequences:

>Translated_336_residues
MSKLNQENKNQKKTQTKFGPTTYSSMDTVRTYLHEIGRVPLLTREEEIVYGKQVQQMMQFLELKEKLEEKLNRTATLLEW
AEHLLVSEKVLKQAIKQGQRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPTRGYKFSTYAYWW
IRQAITRAIAQQARTIRLPIHITEKLNKIKRVQRELIQSLGRSPTPSEIAQALELEPSQIREYLLMARHPISLDLRVGDN
QDTELQELLEDETSSPDDYITGELLRQDINTLLAELSEQQRRVLVLRFGLEDGKEMSLAKVGDKLQLSRERVRQLEHQAL
AFLRRRQAKVREYVAS
>Mature_335_residues
SKLNQENKNQKKTQTKFGPTTYSSMDTVRTYLHEIGRVPLLTREEEIVYGKQVQQMMQFLELKEKLEEKLNRTATLLEWA
EHLLVSEKVLKQAIKQGQRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPTRGYKFSTYAYWWI
RQAITRAIAQQARTIRLPIHITEKLNKIKRVQRELIQSLGRSPTPSEIAQALELEPSQIREYLLMARHPISLDLRVGDNQ
DTELQELLEDETSSPDDYITGELLRQDINTLLAELSEQQRRVLVLRFGLEDGKEMSLAKVGDKLQLSRERVRQLEHQALA
FLRRRQAKVREYVAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=48.9082969432314, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789098, Length=305, Percent_Identity=38.6885245901639, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=28.6324786324786, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788231, Length=221, Percent_Identity=27.6018099547511, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 39257; Mature: 39126

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00715 SIGMA70_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNQENKNQKKTQTKFGPTTYSSMDTVRTYLHEIGRVPLLTREEEIVYGKQVQQMMQF
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LELKEKLEEKLNRTATLLEWAEHLLVSEKVLKQAIKQGQRAKQKMIEANLRLVVAIAKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKRNMEFLDLIQEGTLGLERGVEKFDPTRGYKFSTYAYWWIRQAITRAIAQQARTIRLPI
HHHCHHHHHHHHHCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC
HITEKLNKIKRVQRELIQSLGRSPTPSEIAQALELEPSQIREYLLMARHPISLDLRVGDN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
QDTELQELLEDETSSPDDYITGELLRQDINTLLAELSEQQRRVLVLRFGLEDGKEMSLAK
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCHHHHHH
VGDKLQLSRERVRQLEHQALAFLRRRQAKVREYVAS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLNQENKNQKKTQTKFGPTTYSSMDTVRTYLHEIGRVPLLTREEEIVYGKQVQQMMQF
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LELKEKLEEKLNRTATLLEWAEHLLVSEKVLKQAIKQGQRAKQKMIEANLRLVVAIAKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKRNMEFLDLIQEGTLGLERGVEKFDPTRGYKFSTYAYWWIRQAITRAIAQQARTIRLPI
HHHCHHHHHHHHHCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC
HITEKLNKIKRVQRELIQSLGRSPTPSEIAQALELEPSQIREYLLMARHPISLDLRVGDN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
QDTELQELLEDETSSPDDYITGELLRQDINTLLAELSEQQRRVLVLRFGLEDGKEMSLAK
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCHHHHHH
VGDKLQLSRERVRQLEHQALAFLRRRQAKVREYVAS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]