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The map label for this gene is gacS [H]

Identifier: 113474471

GI number: 113474471

Start: 997127

End: 998314

Strand: Direct

Name: gacS [H]

Synonym: Tery_0615

Alternate gene names: 113474471

Gene position: 997127-998314 (Clockwise)

Preceding gene: 113474470

Following gene: 113474472

Centisome position: 12.87

GC content: 29.71

Gene sequence:

>1188_bases
ATGTCATCAAAAATACTTGTTGTAGATGATGAACCAGATTTTGAATCACTGATCATCCGTAGCTTTAGAAAGAAAATAAA
ACAAAAAGAGCTGGATTTTATTTTTGCCACAAATGGTATAGAAGCACTAGAAATTTTGTCAGAGGGAGTTAATATAGATA
TAGTATTAACTGATATTAATATGCCCTTAATGGATGGGTTAACTTTATTAGATAATCTTCGAGTTTTCTATCCTGATATT
AAGGCAGTAATTGTTTCTGCTTATGATGATCTCGATAAAATTAGGTCTGCGATGAATAGAGGTGCTTTTGATTTTTTGAC
TAAGCCTATAGATTTTCAAGATTTAGAAATTACAATCCAAAAAACATTAGAAAATGTTCAACAGCTCAAAGAAAATAAGC
GTTTAAAGCAAGAAAAACATGAGGCAGAAGCAGCTAATCGAGCAAAAAGTGTATTTCTCGCAAATATGAGCCATGAATTA
CGAACACCATTAAATGCTATTATTGGGTACAGTGAAATTTTACTAGAAGATGCTCATAGTTTAGGTTCTAAAGACTTTAT
TCACGATATTAACAATATTCATACTTCAGGAAAACATTTATTAACGATAATTAATGATATTCTCGATCTTTCAAAAATTG
AGGCTGGTAAGATGGATGTTCACTGGGAAAGTGTTAATATTATGGTATTAATTGAAAATGTTTTAGCAACTATTCAACCT
TTGATTGAACAAAATAATAATATATTAGAAATAATTTGTGAAGATAACCAAGAACCGATAATGGCAGATCCTAATAAAAT
GCGTCAGGTAATTTTAAATCTTTTGAGTAATGCAGCTAAATTTACAGAAAATGGCAAAATAATTTTGAAAGTTAAAGTTG
CTAATAATGATGAAGATTTTATTTCTCAAAATAGAGACTGTAATTTTACAGATAAGGTGTTAATTCTAAGTGTTGCTGAT
ACTGGTATAGGTATATCAGAAGAAAAAATAGATAGTTTATTTCAACCATTTACTCAAGCTGATAATTCAACAACACGAAA
ATATGGAGGAACAGGTTTAGGTTTAGCTATTAGTCGTCACTTCTGTAAAATGATGGGTGGGGATATTACTGTAGTTAGTG
AAGTCAATAAGGGTTCAATATTTACTGTGAAATTTCCTGTTCCTCAAATAATATCTATTGATTCATAA

Upstream 100 bases:

>100_bases
CCCACTTATCCCTTGCTTCTTAAACCATCAACGTAGCTGTCTAATAACTTCGTAAACGGTTGTATAAATTAAAAACAGTT
GGCTTATTAATCAGAATATC

Downstream 100 bases:

>100_bases
AAAATATTAAATAAAAAATTAATATATACCTTACGATTAAGAAAAAAAATTATCTTTTTAGCTTTTACTATACATTAATG
CTTTCATTTTGAAACGACTT

Product: response regulator receiver sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSSKILVVDDEPDFESLIIRSFRKKIKQKELDFIFATNGIEALEILSEGVNIDIVLTDINMPLMDGLTLLDNLRVFYPDI
KAVIVSAYDDLDKIRSAMNRGAFDFLTKPIDFQDLEITIQKTLENVQQLKENKRLKQEKHEAEAANRAKSVFLANMSHEL
RTPLNAIIGYSEILLEDAHSLGSKDFIHDINNIHTSGKHLLTIINDILDLSKIEAGKMDVHWESVNIMVLIENVLATIQP
LIEQNNNILEIICEDNQEPIMADPNKMRQVILNLLSNAAKFTENGKIILKVKVANNDEDFISQNRDCNFTDKVLILSVAD
TGIGISEEKIDSLFQPFTQADNSTTRKYGGTGLGLAISRHFCKMMGGDITVVSEVNKGSIFTVKFPVPQIISIDS

Sequences:

>Translated_395_residues
MSSKILVVDDEPDFESLIIRSFRKKIKQKELDFIFATNGIEALEILSEGVNIDIVLTDINMPLMDGLTLLDNLRVFYPDI
KAVIVSAYDDLDKIRSAMNRGAFDFLTKPIDFQDLEITIQKTLENVQQLKENKRLKQEKHEAEAANRAKSVFLANMSHEL
RTPLNAIIGYSEILLEDAHSLGSKDFIHDINNIHTSGKHLLTIINDILDLSKIEAGKMDVHWESVNIMVLIENVLATIQP
LIEQNNNILEIICEDNQEPIMADPNKMRQVILNLLSNAAKFTENGKIILKVKVANNDEDFISQNRDCNFTDKVLILSVAD
TGIGISEEKIDSLFQPFTQADNSTTRKYGGTGLGLAISRHFCKMMGGDITVVSEVNKGSIFTVKFPVPQIISIDS
>Mature_394_residues
SSKILVVDDEPDFESLIIRSFRKKIKQKELDFIFATNGIEALEILSEGVNIDIVLTDINMPLMDGLTLLDNLRVFYPDIK
AVIVSAYDDLDKIRSAMNRGAFDFLTKPIDFQDLEITIQKTLENVQQLKENKRLKQEKHEAEAANRAKSVFLANMSHELR
TPLNAIIGYSEILLEDAHSLGSKDFIHDINNIHTSGKHLLTIINDILDLSKIEAGKMDVHWESVNIMVLIENVLATIQPL
IEQNNNILEIICEDNQEPIMADPNKMRQVILNLLSNAAKFTENGKIILKVKVANNDEDFISQNRDCNFTDKVLILSVADT
GIGISEEKIDSLFQPFTQADNSTTRKYGGTGLGLAISRHFCKMMGGDITVVSEVNKGSIFTVKFPVPQIISIDS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=334, Percent_Identity=33.8323353293413, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI87081816, Length=255, Percent_Identity=38.0392156862745, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=37.9310344827586, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI48994928, Length=263, Percent_Identity=34.2205323193916, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788713, Length=256, Percent_Identity=32.03125, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=28.9156626506024, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788549, Length=264, Percent_Identity=28.4090909090909, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=28.1512605042017, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786912, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=24.8945147679325, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=239, Percent_Identity=25.5230125523013, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=31.7829457364341, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790346, Length=211, Percent_Identity=27.9620853080569, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=24.1525423728814, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=30.3703703703704, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=33.6, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787894, Length=249, Percent_Identity=24.0963855421687, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788279, Length=240, Percent_Identity=25.8333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=279, Percent_Identity=26.8817204301075, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787487, Length=178, Percent_Identity=27.5280898876405, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=81, Percent_Identity=44.4444444444444, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44302; Mature: 44170

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKILVVDDEPDFESLIIRSFRKKIKQKELDFIFATNGIEALEILSEGVNIDIVLTDIN
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEEEECC
MPLMDGLTLLDNLRVFYPDIKAVIVSAYDDLDKIRSAMNRGAFDFLTKPIDFQDLEITIQ
CCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHH
KTLENVQQLKENKRLKQEKHEAEAANRAKSVFLANMSHELRTPLNAIIGYSEILLEDAHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCHHHHHHHHHHHHHHHHHH
LGSKDFIHDINNIHTSGKHLLTIINDILDLSKIEAGKMDVHWESVNIMVLIENVLATIQP
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEHHHHHHHHHH
LIEQNNNILEIICEDNQEPIMADPNKMRQVILNLLSNAAKFTENGKIILKVKVANNDEDF
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHH
ISQNRDCNFTDKVLILSVADTGIGISEEKIDSLFQPFTQADNSTTRKYGGTGLGLAISRH
HHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHH
FCKMMGGDITVVSEVNKGSIFTVKFPVPQIISIDS
HHHHHCCCEEEEEECCCCCEEEEEECCCCEEECCC
>Mature Secondary Structure 
SSKILVVDDEPDFESLIIRSFRKKIKQKELDFIFATNGIEALEILSEGVNIDIVLTDIN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEEEECC
MPLMDGLTLLDNLRVFYPDIKAVIVSAYDDLDKIRSAMNRGAFDFLTKPIDFQDLEITIQ
CCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHH
KTLENVQQLKENKRLKQEKHEAEAANRAKSVFLANMSHELRTPLNAIIGYSEILLEDAHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCHHHHHHHHHHHHHHHHHH
LGSKDFIHDINNIHTSGKHLLTIINDILDLSKIEAGKMDVHWESVNIMVLIENVLATIQP
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEHHHHHHHHHH
LIEQNNNILEIICEDNQEPIMADPNKMRQVILNLLSNAAKFTENGKIILKVKVANNDEDF
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHH
ISQNRDCNFTDKVLILSVADTGIGISEEKIDSLFQPFTQADNSTTRKYGGTGLGLAISRH
HHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHH
FCKMMGGDITVVSEVNKGSIFTVKFPVPQIISIDS
HHHHHCCCEEEEEECCCCCEEEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]