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The map label for this gene is thiO/thiG [H]

Identifier: 113474177

GI number: 113474177

Start: 438503

End: 439606

Strand: Reverse

Name: thiO/thiG [H]

Synonym: Tery_0284

Alternate gene names: 113474177

Gene position: 439606-438503 (Counterclockwise)

Preceding gene: 113474179

Following gene: 113474175

Centisome position: 5.67

GC content: 34.6

Gene sequence:

>1104_bases
ATGAGTCAATCAAACAAAATCATCATTATTGGTGGCGGTATTATCGGTATATCAATAGCAATAGAACTAAAGTTACGCGG
AGCTAAAGTAACAGTTATTAGCCGAGATTTTCAGCAAATTGCCACAAATGCTGCTGCCGGAATGTTAGCCCCCCAAGCTG
AAGCTATTCCCCCTGGAAAAATGTTAGATTTATGTTTAAAAAGTCGAACATTATATCCAGAATGGGTACAAAAAATAGAA
TCAATTAGTGGTTTAGAAACTGGTTATTGGTCTTGTGGAATTTTATCCCCAGTTTATGCTCTAAAAGATAGAGAAAATTA
TGCTTTACCAAGCCTGAATTATCAATGGCTAAATCAAGTAGCAATCCATCAATATCAACCAGGATTAAGTCCTGAAGTAA
TTGGGGGCTGGTTTTACCCTCAAGATGGGCAAGTAGATAATCGTTATTTATACCAATCAATTATAGCAGCAGCTAAAGAA
TTGAAAGTTGAAATTCAATCAGGAATTGTGACAGAAATAATTCGGAAAAAAGGAAAAATTAAAAGTCTCAAAACATCAAT
AGGTGAAATAGAAGCAGAACATTATGTCTTAGCTACAGGTGCATGGTCGCAGGAGTTATTACCAATTCCCATTTTTCCGA
CAAAAGGGCAAATGTTATCATTAAAAGTGCCCTCATATGTAAGTAGAGAAAATCTATCTTTGCAACAAGTTATATATGGA
AGTCACTGTTATATTGTACCCCGCAAAGATGGCAGAATAATTATTGGTGCAACTTGTGAAAATATCGGATTTGTTGATGG
AAATACTCCGGTAGGAATTCAACAATTATTAGGAAATGCAATTAGATTATATCCAGTGTTAAGGGATTACGAAATCTTAG
AATTTTGGTGGGGTTTTCGTCCTAATACTCCTGATGAAATGCCGATATTAGGAAAAAGTAATTATGATAATTTGACTTTA
GCAACTGGGCATTATCGAAATGGAATTTTATTAGCACCTGTAACAGGAATGTTGATAGCTGATTTAATTTGGAATAATCA
ATCTAACTCCTTATTGACAGCTTTTAATTATTCTCGGTTTATGGAAGTCGGAAGTCAAGTTTGA

Upstream 100 bases:

>100_bases
TAAGAAATGGTATTAATTCTAACTTTTCTTGATAATTATCTATACAATTATTCGTAAAAGTATCAGTTAATTAGTGTAAA
AAAGTCCTGAGTTTACCCTT

Downstream 100 bases:

>100_bases
GGAATTAATATAAAATAACCTTTGATTTAGAATATTCAATTTATTTGAGCAAAATACTTAAAAATTAGTCAATTAACTTA
TAGGGAATTGTGTAGATTAT

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSQSNKIIIIGGGIIGISIAIELKLRGAKVTVISRDFQQIATNAAAGMLAPQAEAIPPGKMLDLCLKSRTLYPEWVQKIE
SISGLETGYWSCGILSPVYALKDRENYALPSLNYQWLNQVAIHQYQPGLSPEVIGGWFYPQDGQVDNRYLYQSIIAAAKE
LKVEIQSGIVTEIIRKKGKIKSLKTSIGEIEAEHYVLATGAWSQELLPIPIFPTKGQMLSLKVPSYVSRENLSLQQVIYG
SHCYIVPRKDGRIIIGATCENIGFVDGNTPVGIQQLLGNAIRLYPVLRDYEILEFWWGFRPNTPDEMPILGKSNYDNLTL
ATGHYRNGILLAPVTGMLIADLIWNNQSNSLLTAFNYSRFMEVGSQV

Sequences:

>Translated_367_residues
MSQSNKIIIIGGGIIGISIAIELKLRGAKVTVISRDFQQIATNAAAGMLAPQAEAIPPGKMLDLCLKSRTLYPEWVQKIE
SISGLETGYWSCGILSPVYALKDRENYALPSLNYQWLNQVAIHQYQPGLSPEVIGGWFYPQDGQVDNRYLYQSIIAAAKE
LKVEIQSGIVTEIIRKKGKIKSLKTSIGEIEAEHYVLATGAWSQELLPIPIFPTKGQMLSLKVPSYVSRENLSLQQVIYG
SHCYIVPRKDGRIIIGATCENIGFVDGNTPVGIQQLLGNAIRLYPVLRDYEILEFWWGFRPNTPDEMPILGKSNYDNLTL
ATGHYRNGILLAPVTGMLIADLIWNNQSNSLLTAFNYSRFMEVGSQV
>Mature_366_residues
SQSNKIIIIGGGIIGISIAIELKLRGAKVTVISRDFQQIATNAAAGMLAPQAEAIPPGKMLDLCLKSRTLYPEWVQKIES
ISGLETGYWSCGILSPVYALKDRENYALPSLNYQWLNQVAIHQYQPGLSPEVIGGWFYPQDGQVDNRYLYQSIIAAAKEL
KVEIQSGIVTEIIRKKGKIKSLKTSIGEIEAEHYVLATGAWSQELLPIPIFPTKGQMLSLKVPSYVSRENLSLQQVIYGS
HCYIVPRKDGRIIIGATCENIGFVDGNTPVGIQQLLGNAIRLYPVLRDYEILEFWWGFRPNTPDEMPILGKSNYDNLTLA
TGHYRNGILLAPVTGMLIADLIWNNQSNSLLTAFNYSRFMEVGSQV

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=241, Percent_Identity=28.2157676348548, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571104, Length=393, Percent_Identity=24.1730279898219, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40766; Mature: 40635

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSNKIIIIGGGIIGISIAIELKLRGAKVTVISRDFQQIATNAAAGMLAPQAEAIPPGK
CCCCCEEEEEECCEEEEEEEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCHH
MLDLCLKSRTLYPEWVQKIESISGLETGYWSCGILSPVYALKDRENYALPSLNYQWLNQV
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCHHHHHHH
AIHQYQPGLSPEVIGGWFYPQDGQVDNRYLYQSIIAAAKELKVEIQSGIVTEIIRKKGKI
HHHHCCCCCCHHHEECEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCH
KSLKTSIGEIEAEHYVLATGAWSQELLPIPIFPTKGQMLSLKVPSYVSRENLSLQQVIYG
HHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHEEEC
SHCYIVPRKDGRIIIGATCENIGFVDGNTPVGIQQLLGNAIRLYPVLRDYEILEFWWGFR
CEEEEEECCCCCEEEEEEECCCEEECCCCCCHHHHHHCCCEEEEHHHCCCHHHHHHHCCC
PNTPDEMPILGKSNYDNLTLATGHYRNGILLAPVTGMLIADLIWNNQSNSLLTAFNYSRF
CCCCCCCCEECCCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHCCCCCCEEEEECHHHH
MEVGSQV
HHHCCCC
>Mature Secondary Structure 
SQSNKIIIIGGGIIGISIAIELKLRGAKVTVISRDFQQIATNAAAGMLAPQAEAIPPGK
CCCCEEEEEECCEEEEEEEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCHH
MLDLCLKSRTLYPEWVQKIESISGLETGYWSCGILSPVYALKDRENYALPSLNYQWLNQV
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCHHHHHHH
AIHQYQPGLSPEVIGGWFYPQDGQVDNRYLYQSIIAAAKELKVEIQSGIVTEIIRKKGKI
HHHHCCCCCCHHHEECEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCH
KSLKTSIGEIEAEHYVLATGAWSQELLPIPIFPTKGQMLSLKVPSYVSRENLSLQQVIYG
HHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHEEEC
SHCYIVPRKDGRIIIGATCENIGFVDGNTPVGIQQLLGNAIRLYPVLRDYEILEFWWGFR
CEEEEEECCCCCEEEEEEECCCEEECCCCCCHHHHHHCCCEEEEHHHCCCHHHHHHHCCC
PNTPDEMPILGKSNYDNLTLATGHYRNGILLAPVTGMLIADLIWNNQSNSLLTAFNYSRF
CCCCCCCCEECCCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHCCCCCCEEEEECHHHH
MEVGSQV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA