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The map label for this gene is topA [H]

Identifier: 113473962

GI number: 113473962

Start: 45501

End: 48125

Strand: Reverse

Name: topA [H]

Synonym: Tery_0027

Alternate gene names: 113473962

Gene position: 48125-45501 (Counterclockwise)

Preceding gene: 113473966

Following gene: 113473956

Centisome position: 0.62

GC content: 41.33

Gene sequence:

>2625_bases
ATGTCAACCCTTGTTATTGTAGAATCTCCGACTAAAGCCCGCACCATTCGCAACTATCTACCCTCCGACTACCGCGTGGA
AGCATCTATGGGTCATGTGCGTGACCTACCACCTTCTGCCGAGGAAATACCAGAAAAATTCAAAGGAGAAAAGTGGGCAC
AGTTAGGGGTAAATGTAGAGTCAGATTTTGAACCAATCTACGTTGTCCCCAAAGACAAAAAGAAAACTGTCAAAGAACTC
AAAGATGCTCTGAAAGAAGTTGATGAATTACTTCTAGCCACAGATGAAGACCGTGAAGGAGAAAGTATAAGTTGGCATTT
ATTGCAACTACTAAAACCAAAAGTTCCTACTAAACGGATGGTCTTCCATGAAATTACTCCAGAAGCTATCCGTAGAGCAA
TTGATAATTGTCGTAACATAGATGAGCAGTTAGTCAGGGCACAAGAAACTCGTCGTATTCTAGACCGCCTCTACGGTTAT
ACTCTCTCACCTGTACTTTGGAAAAAAATTGCCAGAGGTCTTTCTGCAGGTAGAGTACAGTCAGTAGCAGTCCGACTCTT
GGTTAACCGAGAACGCCAACGTCAGGCTTTTAAGCGAGGAGGTTATTGGGATTTAAAGGCTAGTTTAGAACAAGAAAAAA
CTCCATTTGAGTCTAAACTTGTGACCCTTGGGGGGACAAAAATAGCTACTGGTAATGACTTTGATGAAAATACAGGCAAA
ATAGTTGAAGGTCGTAATGTTATTTTATTGGACGAAGCTCATGCAGAAGCTCTCAAAGAACAACTGCAACATAAGCCTTG
GACTGTTAATAGTTTTGATGAACGAGCTGTTAGACGTAAACCATCTCCACCATTTACTACTTCTACTTTACAACAGGAAG
CTAACCGCAAACTACGAATGGGTGCTAGGCAAACAATGCGGACAGCCCAAAATTTATATGAACAGGGCTTTATCACTTAT
ATGCGGACAGACTCAGTACATTTGTCTGATGAGGCGATCGCTGCTGCCCGAAGTTGTGTTGAGAAAATGTATGGCCCGGA
ATATCTAAGTTCTGAACCTAGGCAATACACTACTAAAAGTAAGGGGGCTCAGGAAGCTCACGAAGCTATTCGCCCTGCAG
GTAAAACTTTTCGCACTCCCCAGGAAACAGGATTGAAAAATCAAGAATTTGCTCTCTATGACTTAATTTGGAAAAGAACA
GTAGCTTGTCAAATGGCTGATTCTCGTCAAACCCATATTACAGTTAATTTACAAGTAGAAGATGCTGGTTTTCGTTCTAA
TGGAAAACGGATAGACTTTCCTGGGTTCCTCCGGGCTTATGTTGAAGGGTCTGATGACCCCGAAGTATCTTTGGAAAACC
AAGAAGTACCATTGCCTGCTATAAAACAGGGAGACCATCCTGCTTGCACAGAAATAGAAGTAGTGGGTCATGAAACTCAG
CCACCTGCCAGGTTTACCGAAGCGTCTTTGGTAAAAACTTTGGAAAGTGAAGGTATCGGCCGTCCGAGTACCTACGCTAC
TGTTATTGGTACTATTGTTGATCGTGGTTATGCCAAACTCCAGAGTAATGCTTTAGTTCCAACATTTACTGCTTTTGCTG
TTACAACTTTACTGGAAAAACATTTTCCTGAGTTTGTAGATGTCAAGTTTACTGCCCGTATGGAACAAACCCTCGATGAC
ATTTCTATGGGTGAAGCTCAGTGGGTACCTTACCTTAAAAAATTTTATTTTGGGGAAAGTGGACTTGATACTCAAATCAA
GGAACAGGAAAATCAAATTGACCCTAAGTTGGCCCGAACTATTGAGTTAGATAATTTGGATGCTAATTCAGAGATGACTT
ATCGTATATGTATTGGTAAGTTTGGTGCTTATATTGAGGCAGAAAATGGTGAGAATATAGTCAAAGCTTCTATTCCTGAA
GATTTAACTCCATCAGACCTAGACCCAGAACAAATAGAGAAACTGTTGAAGCAAAAAACAGAGGGTCCGCAGGAGTTGGG
AATTCATCCAGAAGAAGATAAGTCTATCTATATAATGACTGGTCGTTACGGTCCTTATGTGCAGTTGGGTGATGATTCTG
AGGGTAGTAAGAGGAAACCAAAGCGAGCTTCTTTGCCAAAGGGTGTCAATATGGAGGATGTAACTTTGGATCTAGCGGTA
GGGTTATTATCTTTACCTCGTACTTTGGGAACTCATCCAGAAACTGGTTGCAAAATTCAAGCTAATTTAGGACGTTTTGG
CCCATATATCGTTCACGACCAGGGAAAGGAGGATGGTAAAGACTATCGTTCTTTAAAGGTTAAGGATGATGTTTTGACTA
TTACTTTGGAACGTGCTTTGGAGTTATTAGCTCAACCGAAAAGATCGAGGAGAGGTAGTGCTAAAGCTGTACTTCCCCTC
AAAGATTTGGGTAAACATCCTGAAGATGGAGAAACAGTAGGTGTTTATGATGGTCGATATGGACTTTATGTGAAGCATGG
TAAGGTTAATGCTTCTTTGCCTAAGGATATGTCTGTTGAGGATGTAACTTGGGAAAAGGCTCTAGAGCTTTTGCATGCTA
AGGCTAGTAGTAAAAAATCTGCTCGTGGTAGGAAATCCACAAAGTCAAAAGCCGAAAATAACTAG

Upstream 100 bases:

>100_bases
ATCAACCACCCGAAACATTTATGACAGGTACATAACTATCTATTCAATCTAATACTAACATACAAAAATTAATCTCTTAT
AATACCTAATATAAGGTCTT

Downstream 100 bases:

>100_bases
GGTTTGTTCTTCTTGATCTTTTTAAGGAGTAGGGGGGTCGGCAGGGCATTTTTATTCTCAAAGAAAGCTATCGCATAACT
ACCAAAATTGAAGGCAGTTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 874; Mature: 873

Protein sequence:

>874_residues
MSTLVIVESPTKARTIRNYLPSDYRVEASMGHVRDLPPSAEEIPEKFKGEKWAQLGVNVESDFEPIYVVPKDKKKTVKEL
KDALKEVDELLLATDEDREGESISWHLLQLLKPKVPTKRMVFHEITPEAIRRAIDNCRNIDEQLVRAQETRRILDRLYGY
TLSPVLWKKIARGLSAGRVQSVAVRLLVNRERQRQAFKRGGYWDLKASLEQEKTPFESKLVTLGGTKIATGNDFDENTGK
IVEGRNVILLDEAHAEALKEQLQHKPWTVNSFDERAVRRKPSPPFTTSTLQQEANRKLRMGARQTMRTAQNLYEQGFITY
MRTDSVHLSDEAIAAARSCVEKMYGPEYLSSEPRQYTTKSKGAQEAHEAIRPAGKTFRTPQETGLKNQEFALYDLIWKRT
VACQMADSRQTHITVNLQVEDAGFRSNGKRIDFPGFLRAYVEGSDDPEVSLENQEVPLPAIKQGDHPACTEIEVVGHETQ
PPARFTEASLVKTLESEGIGRPSTYATVIGTIVDRGYAKLQSNALVPTFTAFAVTTLLEKHFPEFVDVKFTARMEQTLDD
ISMGEAQWVPYLKKFYFGESGLDTQIKEQENQIDPKLARTIELDNLDANSEMTYRICIGKFGAYIEAENGENIVKASIPE
DLTPSDLDPEQIEKLLKQKTEGPQELGIHPEEDKSIYIMTGRYGPYVQLGDDSEGSKRKPKRASLPKGVNMEDVTLDLAV
GLLSLPRTLGTHPETGCKIQANLGRFGPYIVHDQGKEDGKDYRSLKVKDDVLTITLERALELLAQPKRSRRGSAKAVLPL
KDLGKHPEDGETVGVYDGRYGLYVKHGKVNASLPKDMSVEDVTWEKALELLHAKASSKKSARGRKSTKSKAENN

Sequences:

>Translated_874_residues
MSTLVIVESPTKARTIRNYLPSDYRVEASMGHVRDLPPSAEEIPEKFKGEKWAQLGVNVESDFEPIYVVPKDKKKTVKEL
KDALKEVDELLLATDEDREGESISWHLLQLLKPKVPTKRMVFHEITPEAIRRAIDNCRNIDEQLVRAQETRRILDRLYGY
TLSPVLWKKIARGLSAGRVQSVAVRLLVNRERQRQAFKRGGYWDLKASLEQEKTPFESKLVTLGGTKIATGNDFDENTGK
IVEGRNVILLDEAHAEALKEQLQHKPWTVNSFDERAVRRKPSPPFTTSTLQQEANRKLRMGARQTMRTAQNLYEQGFITY
MRTDSVHLSDEAIAAARSCVEKMYGPEYLSSEPRQYTTKSKGAQEAHEAIRPAGKTFRTPQETGLKNQEFALYDLIWKRT
VACQMADSRQTHITVNLQVEDAGFRSNGKRIDFPGFLRAYVEGSDDPEVSLENQEVPLPAIKQGDHPACTEIEVVGHETQ
PPARFTEASLVKTLESEGIGRPSTYATVIGTIVDRGYAKLQSNALVPTFTAFAVTTLLEKHFPEFVDVKFTARMEQTLDD
ISMGEAQWVPYLKKFYFGESGLDTQIKEQENQIDPKLARTIELDNLDANSEMTYRICIGKFGAYIEAENGENIVKASIPE
DLTPSDLDPEQIEKLLKQKTEGPQELGIHPEEDKSIYIMTGRYGPYVQLGDDSEGSKRKPKRASLPKGVNMEDVTLDLAV
GLLSLPRTLGTHPETGCKIQANLGRFGPYIVHDQGKEDGKDYRSLKVKDDVLTITLERALELLAQPKRSRRGSAKAVLPL
KDLGKHPEDGETVGVYDGRYGLYVKHGKVNASLPKDMSVEDVTWEKALELLHAKASSKKSARGRKSTKSKAENN
>Mature_873_residues
STLVIVESPTKARTIRNYLPSDYRVEASMGHVRDLPPSAEEIPEKFKGEKWAQLGVNVESDFEPIYVVPKDKKKTVKELK
DALKEVDELLLATDEDREGESISWHLLQLLKPKVPTKRMVFHEITPEAIRRAIDNCRNIDEQLVRAQETRRILDRLYGYT
LSPVLWKKIARGLSAGRVQSVAVRLLVNRERQRQAFKRGGYWDLKASLEQEKTPFESKLVTLGGTKIATGNDFDENTGKI
VEGRNVILLDEAHAEALKEQLQHKPWTVNSFDERAVRRKPSPPFTTSTLQQEANRKLRMGARQTMRTAQNLYEQGFITYM
RTDSVHLSDEAIAAARSCVEKMYGPEYLSSEPRQYTTKSKGAQEAHEAIRPAGKTFRTPQETGLKNQEFALYDLIWKRTV
ACQMADSRQTHITVNLQVEDAGFRSNGKRIDFPGFLRAYVEGSDDPEVSLENQEVPLPAIKQGDHPACTEIEVVGHETQP
PARFTEASLVKTLESEGIGRPSTYATVIGTIVDRGYAKLQSNALVPTFTAFAVTTLLEKHFPEFVDVKFTARMEQTLDDI
SMGEAQWVPYLKKFYFGESGLDTQIKEQENQIDPKLARTIELDNLDANSEMTYRICIGKFGAYIEAENGENIVKASIPED
LTPSDLDPEQIEKLLKQKTEGPQELGIHPEEDKSIYIMTGRYGPYVQLGDDSEGSKRKPKRASLPKGVNMEDVTLDLAVG
LLSLPRTLGTHPETGCKIQANLGRFGPYIVHDQGKEDGKDYRSLKVKDDVLTITLERALELLAQPKRSRRGSAKAVLPLK
DLGKHPEDGETVGVYDGRYGLYVKHGKVNASLPKDMSVEDVTWEKALELLHAKASSKKSARGRKSTKSKAENN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=493, Percent_Identity=25.5578093306288, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI4507635, Length=517, Percent_Identity=24.3713733075435, Blast_Score=110, Evalue=7e-24,
Organism=Escherichia coli, GI1787529, Length=589, Percent_Identity=39.2190152801358, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1788061, Length=618, Percent_Identity=24.2718446601942, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=496, Percent_Identity=25.4032258064516, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=592, Percent_Identity=24.8310810810811, Blast_Score=112, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=578, Percent_Identity=23.1833910034602, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585251, Length=500, Percent_Identity=27.2, Blast_Score=119, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640096, Length=528, Percent_Identity=22.7272727272727, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98269; Mature: 98137

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00396 TOPOISOMERASE_I_PROK ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVIVESPTKARTIRNYLPSDYRVEASMGHVRDLPPSAEEIPEKFKGEKWAQLGVNVE
CCEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCC
SDFEPIYVVPKDKKKTVKELKDALKEVDELLLATDEDREGESISWHLLQLLKPKVPTKRM
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITPEAIRRAIDNCRNIDEQLVRAQETRRILDRLYGYTLSPVLWKKIARGLSAGRVQ
HHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHH
SVAVRLLVNRERQRQAFKRGGYWDLKASLEQEKTPFESKLVTLGGTKIATGNDFDENTGK
HHHHHHHHCCHHHHHHHHCCCCEEECCCHHHCCCCHHHEEEEECCCEEECCCCCCCCCCC
IVEGRNVILLDEAHAEALKEQLQHKPWTVNSFDERAVRRKPSPPFTTSTLQQEANRKLRM
EECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GARQTMRTAQNLYEQGFITYMRTDSVHLSDEAIAAARSCVEKMYGPEYLSSEPRQYTTKS
HHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHC
KGAQEAHEAIRPAGKTFRTPQETGLKNQEFALYDLIWKRTVACQMADSRQTHITVNLQVE
CCHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
DAGFRSNGKRIDFPGFLRAYVEGSDDPEVSLENQEVPLPAIKQGDHPACTEIEVVGHETQ
CCCCCCCCCEECCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCC
PPARFTEASLVKTLESEGIGRPSTYATVIGTIVDRGYAKLQSNALVPTFTAFAVTTLLEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HFPEFVDVKFTARMEQTLDDISMGEAQWVPYLKKFYFGESGLDTQIKEQENQIDPKLART
HCCHHCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHE
IELDNLDANSEMTYRICIGKFGAYIEAENGENIVKASIPEDLTPSDLDPEQIEKLLKQKT
EEECCCCCCCCEEEEEEEECCCCEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHC
EGPQELGIHPEEDKSIYIMTGRYGPYVQLGDDSEGSKRKPKRASLPKGVNMEDVTLDLAV
CCHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GLLSLPRTLGTHPETGCKIQANLGRFGPYIVHDQGKEDGKDYRSLKVKDDVLTITLERAL
HHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCHHHCEEEECCEEEEEHHHHH
ELLAQPKRSRRGSAKAVLPLKDLGKHPEDGETVGVYDGRYGLYVKHGKVNASLPKDMSVE
HHHHCCHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECCCEEEEEEECEECCCCCCCCCCC
DVTWEKALELLHAKASSKKSARGRKSTKSKAENN
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
STLVIVESPTKARTIRNYLPSDYRVEASMGHVRDLPPSAEEIPEKFKGEKWAQLGVNVE
CEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCC
SDFEPIYVVPKDKKKTVKELKDALKEVDELLLATDEDREGESISWHLLQLLKPKVPTKRM
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITPEAIRRAIDNCRNIDEQLVRAQETRRILDRLYGYTLSPVLWKKIARGLSAGRVQ
HHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHH
SVAVRLLVNRERQRQAFKRGGYWDLKASLEQEKTPFESKLVTLGGTKIATGNDFDENTGK
HHHHHHHHCCHHHHHHHHCCCCEEECCCHHHCCCCHHHEEEEECCCEEECCCCCCCCCCC
IVEGRNVILLDEAHAEALKEQLQHKPWTVNSFDERAVRRKPSPPFTTSTLQQEANRKLRM
EECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GARQTMRTAQNLYEQGFITYMRTDSVHLSDEAIAAARSCVEKMYGPEYLSSEPRQYTTKS
HHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHC
KGAQEAHEAIRPAGKTFRTPQETGLKNQEFALYDLIWKRTVACQMADSRQTHITVNLQVE
CCHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
DAGFRSNGKRIDFPGFLRAYVEGSDDPEVSLENQEVPLPAIKQGDHPACTEIEVVGHETQ
CCCCCCCCCEECCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCC
PPARFTEASLVKTLESEGIGRPSTYATVIGTIVDRGYAKLQSNALVPTFTAFAVTTLLEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HFPEFVDVKFTARMEQTLDDISMGEAQWVPYLKKFYFGESGLDTQIKEQENQIDPKLART
HCCHHCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHE
IELDNLDANSEMTYRICIGKFGAYIEAENGENIVKASIPEDLTPSDLDPEQIEKLLKQKT
EEECCCCCCCCEEEEEEEECCCCEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHC
EGPQELGIHPEEDKSIYIMTGRYGPYVQLGDDSEGSKRKPKRASLPKGVNMEDVTLDLAV
CCHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GLLSLPRTLGTHPETGCKIQANLGRFGPYIVHDQGKEDGKDYRSLKVKDDVLTITLERAL
HHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCHHHCEEEECCEEEEEHHHHH
ELLAQPKRSRRGSAKAVLPLKDLGKHPEDGETVGVYDGRYGLYVKHGKVNASLPKDMSVE
HHHHCCHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECCCEEEEEEECEECCCCCCCCCCC
DVTWEKALELLHAKASSKKSARGRKSTKSKAENN
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]