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The map label for this gene is est [H]

Identifier: 110835315

GI number: 110835315

Start: 2779097

End: 2779987

Strand: Direct

Name: est [H]

Synonym: ABO_2454

Alternate gene names: 110835315

Gene position: 2779097-2779987 (Clockwise)

Preceding gene: 110835314

Following gene: 110835316

Centisome position: 89.07

GC content: 56.57

Gene sequence:

>891_bases
ATGAGCCTTCAAGCCCGCCTCATCAAAGCCGTGACCAAGCGCACCATCAAACGCTCCGGTCTCAACCAAGACCAACTAGT
ACGGCATCTGCGTAAGGTATTCAACGAAACCCCTGTTCTGACCCTATTACCCCGTGGAGTAAAACTCAGTCGCGTGGAGC
ACCCCGCTTTTACCGGTGATCGAATCAGCGTGCAGCGTCCAGAAATGGCGGTGCTTTACCTGCATGGCGGCGCCTACATT
GGCGGCATCACCAAGACCTACCACAACTTAGCCGGGCGGTTGGCAAAAAAATTAAACGCTGAGGTATTTTTACCCGTCTA
TCCGTTTGCCCCAGAGCATCCTTACCCGGCCGCAGTGAATCGAGTCATGGAGGCCTACGAATACCTATTAAGCCTAGGCA
AGCAACCGCAGGATATCGTTATTGCCGGCGATTCCGCCGGTGGTGGGCTCACCTTGGCCACGCTGCTGCATATCCGCGAC
AAAGGCCTGGAACAACCCCGCTGCGCGGTGACATTTTCGCCCGCCAGCAACGCCTTTCCTGACGACAGTATCCTGGAGGC
CCTAGACCCCAGCGATGCGATGCTGTCTGCAGACATCATTCGCACTGCTATCGAGATCTATACCCCCAACCCAGAAGACC
GCAGCCAGCCCTACGCCTCCCCCTGCCTAGGAGACTACACCAACATCTGCCCGCTACTGATCACCGCCAGCACCGACGAA
CTGCTTTATGCGGATGGCAAGCGAGTGAAGCGCGTGGCGGAAAAGGCGGGCGTCAAGGTGACATGGATAGAGCGCCCCGG
CGTATTCCATGTATGGCCGGTCATGGTGCCCTTCCTGCCAGAGGCGAACAAAGACCTGAAACGGATTGTCGCATTCATTA
AAGAAGCATAG

Upstream 100 bases:

>100_bases
GGCCAGTGCCAACTGGCCCGATCAACCCTGCCAATACGCCCCCTCACTCGCTAGTGTTGGGGCGCCATAACACAACAACG
AACCAGATAACGGACAAACT

Downstream 100 bases:

>100_bases
GCGCGAGCGCTTAAGGCGATAAAAACCAAGCCCGCAGTGCTCCAACCCTGGTTGAAGTTTTCGGCATTAATCATTGAACG
TTTCTGTCATTGCTCTGCCG

Product: lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSLQARLIKAVTKRTIKRSGLNQDQLVRHLRKVFNETPVLTLLPRGVKLSRVEHPAFTGDRISVQRPEMAVLYLHGGAYI
GGITKTYHNLAGRLAKKLNAEVFLPVYPFAPEHPYPAAVNRVMEAYEYLLSLGKQPQDIVIAGDSAGGGLTLATLLHIRD
KGLEQPRCAVTFSPASNAFPDDSILEALDPSDAMLSADIIRTAIEIYTPNPEDRSQPYASPCLGDYTNICPLLITASTDE
LLYADGKRVKRVAEKAGVKVTWIERPGVFHVWPVMVPFLPEANKDLKRIVAFIKEA

Sequences:

>Translated_296_residues
MSLQARLIKAVTKRTIKRSGLNQDQLVRHLRKVFNETPVLTLLPRGVKLSRVEHPAFTGDRISVQRPEMAVLYLHGGAYI
GGITKTYHNLAGRLAKKLNAEVFLPVYPFAPEHPYPAAVNRVMEAYEYLLSLGKQPQDIVIAGDSAGGGLTLATLLHIRD
KGLEQPRCAVTFSPASNAFPDDSILEALDPSDAMLSADIIRTAIEIYTPNPEDRSQPYASPCLGDYTNICPLLITASTDE
LLYADGKRVKRVAEKAGVKVTWIERPGVFHVWPVMVPFLPEANKDLKRIVAFIKEA
>Mature_295_residues
SLQARLIKAVTKRTIKRSGLNQDQLVRHLRKVFNETPVLTLLPRGVKLSRVEHPAFTGDRISVQRPEMAVLYLHGGAYIG
GITKTYHNLAGRLAKKLNAEVFLPVYPFAPEHPYPAAVNRVMEAYEYLLSLGKQPQDIVIAGDSAGGGLTLATLLHIRDK
GLEQPRCAVTFSPASNAFPDDSILEALDPSDAMLSADIIRTAIEIYTPNPEDRSQPYASPCLGDYTNICPLLITASTDEL
LYADGKRVKRVAEKAGVKVTWIERPGVFHVWPVMVPFLPEANKDLKRIVAFIKEA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533410, Length=124, Percent_Identity=31.4516129032258, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=124, Percent_Identity=31.4516129032258, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32707; Mature: 32576

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQARLIKAVTKRTIKRSGLNQDQLVRHLRKVFNETPVLTLLPRGVKLSRVEHPAFTGD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCC
RISVQRPEMAVLYLHGGAYIGGITKTYHNLAGRLAKKLNAEVFLPVYPFAPEHPYPAAVN
CEEEECCCEEEEEEECCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
RVMEAYEYLLSLGKQPQDIVIAGDSAGGGLTLATLLHIRDKGLEQPRCAVTFSPASNAFP
HHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
DDSILEALDPSDAMLSADIIRTAIEIYTPNPEDRSQPYASPCLGDYTNICPLLITASTDE
CHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCC
LLYADGKRVKRVAEKAGVKVTWIERPGVFHVWPVMVPFLPEANKDLKRIVAFIKEA
EEECCCHHHHHHHHHCCCEEEEEECCCEEEEHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SLQARLIKAVTKRTIKRSGLNQDQLVRHLRKVFNETPVLTLLPRGVKLSRVEHPAFTGD
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCC
RISVQRPEMAVLYLHGGAYIGGITKTYHNLAGRLAKKLNAEVFLPVYPFAPEHPYPAAVN
CEEEECCCEEEEEEECCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
RVMEAYEYLLSLGKQPQDIVIAGDSAGGGLTLATLLHIRDKGLEQPRCAVTFSPASNAFP
HHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
DDSILEALDPSDAMLSADIIRTAIEIYTPNPEDRSQPYASPCLGDYTNICPLLITASTDE
CHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCC
LLYADGKRVKRVAEKAGVKVTWIERPGVFHVWPVMVPFLPEANKDLKRIVAFIKEA
EEECCCHHHHHHHHHCCCEEEEEECCCEEEEHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]