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The map label for this gene is lysRS [H]

Identifier: 110835308

GI number: 110835308

Start: 2771871

End: 2772806

Strand: Direct

Name: lysRS [H]

Synonym: ABO_2447

Alternate gene names: 110835308

Gene position: 2771871-2772806 (Clockwise)

Preceding gene: 110835307

Following gene: 110835309

Centisome position: 88.84

GC content: 58.76

Gene sequence:

>936_bases
ATGAGCAACTGGCAGCCCACCGCCTCTTTGCAGGCCATCAAGGCGCGCGCTGAACTACTAAGCACAGTGCGCGCCTTTTT
TGATGCCCGCCAAGTACTGGAAGTGGACACCCCGCAGCTGGCCCGTTACGGCGTCAGCGACCCACACATCCAGTGTATTG
CCGTGCCCGGCTACGGTTATCTGCAAAGCTCGCCGGAATACCATATGAAGCGTCTGCTCGCCGCTGGCAGTGGGCCTATA
TACCAGCTGTGCAAAGCCTTCCGCGAGGGCGAGGCTGGGGGGCGCCATAACCCTGAGTTCACCATGTTAGAATGGTACCG
GCCCGGTTTTTCCCTGAATCAGTTAGTTAGCGAATGCCTAGCGTTGTTTGCTGAACTGTTTCCCGGCATCAGGGCAAAGA
AATACCCCTTTCGTGACCTGTTCAAGAACATTACAGGCTTGGACCCACTTACCGTCGATGACGCAGAGCTAATCCATTAC
GCGGTATTCCACGGCGCGCCTACTGGCCTGAACAAAACCGCCGCGGTGGACTATCTGATGGCCACTCAGGTGGAAACGGC
CCTCCCTGCCGAACAACTGTCTGTGGTGACCGACTTCCCCGGCTGGGCCGCTGCGCTGGCCCAGACCCATAAGGACAAAG
ACGGTGCCATCGTGGCCAAGCGATTCGAGGTTTACTACCGGGGCCTGGAGCTGGCCAATGGCTACCAGGAACTCACTGAC
GCGCAAGAGCAGTCACAGCGCTTTCAACGGGACAATGCTCTGCGGCTCGAACAAGGATTGCCCCCCATGGCTCCCGATGC
TTATTTGCTCGACGCCATGACATCCGGACTTCCAGAATGTAGTGGTGTCGCCGTTGGCGTTGAGCGGCTACTCATGGCCC
AGCAGCCGCCCCTGACCATCGACCAGGTGCTGACGTTCCCCCACCCAAGAGCGTAA

Upstream 100 bases:

>100_bases
CCGGCGCAGTGGTACGTGTCCCGCTGTTCGTACAGACCGGTGAAATCGTCAAAGTCGACACCCGTACCGGCGATTACGTG
GGCCGCATCAAACAGTAGCC

Downstream 100 bases:

>100_bases
CGTCGCCCTCTCGCTTTTTGCTTAGTCATCGAACCCAACACAAGGAACGCTTGGCCATTGTGATAAAGTAGCCATCAATG
TCCGAACCGGTAAGCACTAT

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSNWQPTASLQAIKARAELLSTVRAFFDARQVLEVDTPQLARYGVSDPHIQCIAVPGYGYLQSSPEYHMKRLLAAGSGPI
YQLCKAFREGEAGGRHNPEFTMLEWYRPGFSLNQLVSECLALFAELFPGIRAKKYPFRDLFKNITGLDPLTVDDAELIHY
AVFHGAPTGLNKTAAVDYLMATQVETALPAEQLSVVTDFPGWAAALAQTHKDKDGAIVAKRFEVYYRGLELANGYQELTD
AQEQSQRFQRDNALRLEQGLPPMAPDAYLLDAMTSGLPECSGVAVGVERLLMAQQPPLTIDQVLTFPHPRA

Sequences:

>Translated_311_residues
MSNWQPTASLQAIKARAELLSTVRAFFDARQVLEVDTPQLARYGVSDPHIQCIAVPGYGYLQSSPEYHMKRLLAAGSGPI
YQLCKAFREGEAGGRHNPEFTMLEWYRPGFSLNQLVSECLALFAELFPGIRAKKYPFRDLFKNITGLDPLTVDDAELIHY
AVFHGAPTGLNKTAAVDYLMATQVETALPAEQLSVVTDFPGWAAALAQTHKDKDGAIVAKRFEVYYRGLELANGYQELTD
AQEQSQRFQRDNALRLEQGLPPMAPDAYLLDAMTSGLPECSGVAVGVERLLMAQQPPLTIDQVLTFPHPRA
>Mature_310_residues
SNWQPTASLQAIKARAELLSTVRAFFDARQVLEVDTPQLARYGVSDPHIQCIAVPGYGYLQSSPEYHMKRLLAAGSGPIY
QLCKAFREGEAGGRHNPEFTMLEWYRPGFSLNQLVSECLALFAELFPGIRAKKYPFRDLFKNITGLDPLTVDDAELIHYA
VFHGAPTGLNKTAAVDYLMATQVETALPAEQLSVVTDFPGWAAALAQTHKDKDGAIVAKRFEVYYRGLELANGYQELTDA
QEQSQRFQRDNALRLEQGLPPMAPDAYLLDAMTSGLPECSGVAVGVERLLMAQQPPLTIDQVLTFPHPRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=323, Percent_Identity=40.5572755417957, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789256, Length=325, Percent_Identity=27.0769230769231, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790571, Length=325, Percent_Identity=26.7692307692308, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 34418; Mature: 34286

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNWQPTASLQAIKARAELLSTVRAFFDARQVLEVDTPQLARYGVSDPHIQCIAVPGYGY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCC
LQSSPEYHMKRLLAAGSGPIYQLCKAFREGEAGGRHNPEFTMLEWYRPGFSLNQLVSECL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ALFAELFPGIRAKKYPFRDLFKNITGLDPLTVDDAELIHYAVFHGAPTGLNKTAAVDYLM
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
ATQVETALPAEQLSVVTDFPGWAAALAQTHKDKDGAIVAKRFEVYYRGLELANGYQELTD
HHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQEQSQRFQRDNALRLEQGLPPMAPDAYLLDAMTSGLPECSGVAVGVERLLMAQQPPLTI
HHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
DQVLTFPHPRA
HHHHCCCCCCC
>Mature Secondary Structure 
SNWQPTASLQAIKARAELLSTVRAFFDARQVLEVDTPQLARYGVSDPHIQCIAVPGYGY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCC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HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA