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The map label for this gene is gltD [H]

Identifier: 110835089

GI number: 110835089

Start: 2541034

End: 2542455

Strand: Reverse

Name: gltD [H]

Synonym: ABO_2228

Alternate gene names: 110835089

Gene position: 2542455-2541034 (Counterclockwise)

Preceding gene: 110835090

Following gene: 110835088

Centisome position: 81.49

GC content: 54.29

Gene sequence:

>1422_bases
ATGGCTGAACGTTTAAGCAATAATTTCCAGTTTCTTGACGTGCCCCGGCACGATCCGAAGAAAAAGGATATTGACGACCG
TAAGCACAAGTACGAGGAAATCTACTACCCATTTGAAACCCGGGAAGTAGAAAATCAGGCGCATCGCTGCCTGCATTGCG
GTAACCCGTACTGTGAATGGAAGTGCCCGGTCCATAACTACATTCCTAACTGGTTGAAGCTGATCAGTGAAGGCAACCTG
CTGGAAGCGGTGGAGTTGAGTCACCAGACCAACTCCCTGCCAGAAGTGTGTGGCCGGGTGTGTCCGCAGGACCGTCTGTG
TGAAGGGGCCTGTACTCTAAATGATGGCTTTGGCGCGGTAACGATTGGTGCCACTGAGAAGTACATTACCGATACTGCGC
TGGCTATGGGCTGGCGCCCGGATATGTCCAAGGTAGTGTGGACCGACAAGAAGGTCGCAGTGATTGGTGCTGGCCCTGCC
GGTATCGGCTGTGCCGATGTGTTGGTGCGCAGTGGCGTGAAGCCGGTGGTATTCGATCGCTATGAGGAAATTGGCGGCCT
GCTGACTTTTGGTATTCCCGAGTTCAAGTTAGAAAAGCCGGTCATGGCCAAACGCCGTGAAGTCTTTGAGGGCATGGGGG
TGGAATTCCGTCTGGGCGTAGAGGTCGGTAAGGACATCACCATTGATGAACTGCTTGCCGACTACGATGCCGTCTTCATG
GGCATGGGCACTTACACCTATATGAAAGGTGGTTTCCCGGGTGAAGAGTTGGACGGTGTGTATGAAGCTCTGCCGTTCCT
GATCTCTAACGCTAACCGTAATTTGGGCTTTGAAAAAGACGAAGCGGACTTCATCAATATGCAGGGCAAGCGTGTGGTAG
TGCTGGGTGGTGGTGATACCGCTATGGACTGTAATCGCACCTCAATCCGTCAGCAGGCGCAAAGCGTTAGCTGTGCCTAC
CGTCGAGATGAAGAAAACATGCCCGGTTCACGCAAGGAAGTGACCAACGCCAAAGAAGAAGGCGTGCAGTTCCTGTTCAA
CCGCCAACCCATTGAAGTGGTGGGTGAAGACGGCAAGGTGGTCGGCGTTAAGGTAGTGGAAACCAAGCTTGGCGAGCCAG
ACAACAATGGCCGCCGCCGCCCTGAGCCGATTCCGGGCAGTGAAGAAATTCTGCCGGCGGATGCGGTGATTGTGGCGTTC
GGTTTCCGCCCGAGCCCAGCGGATTGGTTTGGTGAGAAAAGCATCAATACCGACGATTCCGGCCGCGTGGTCGCCGTGGA
AGAACAGGCATTCCCGTTCCAGACGAGCAATGAGAAGATCTTTGCAGGTGGTGACATGGTGCGTGGCTCGGACTTGGTGG
TGACTGCCATTTGGGAAGGCCGTGAAGCTGCCAAAGGGATTCTTGACTACTTGGATGTGTGA

Upstream 100 bases:

>100_bases
ATGCCATGAGCCGCAAGTTCTGGTTGGTTAAGCCCAAGGCCGCAAGCCTGGAAAACCTGCTGCAGAGCACGCGCGCCAAT
CCGGCATAACGGGGGATACC

Downstream 100 bases:

>100_bases
GTTGCGCAACAGGCAACATGCTGTACGCAACATAGGGCCTGCTTTTGCAGGCCCTTTTTCTATATGATGTTGAAGATTTT
TATTTTCGCGCGTTGCGTGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAERLSNNFQFLDVPRHDPKKKDIDDRKHKYEEIYYPFETREVENQAHRCLHCGNPYCEWKCPVHNYIPNWLKLISEGNL
LEAVELSHQTNSLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGATEKYITDTALAMGWRPDMSKVVWTDKKVAVIGAGPA
GIGCADVLVRSGVKPVVFDRYEEIGGLLTFGIPEFKLEKPVMAKRREVFEGMGVEFRLGVEVGKDITIDELLADYDAVFM
GMGTYTYMKGGFPGEELDGVYEALPFLISNANRNLGFEKDEADFINMQGKRVVVLGGGDTAMDCNRTSIRQQAQSVSCAY
RRDEENMPGSRKEVTNAKEEGVQFLFNRQPIEVVGEDGKVVGVKVVETKLGEPDNNGRRRPEPIPGSEEILPADAVIVAF
GFRPSPADWFGEKSINTDDSGRVVAVEEQAFPFQTSNEKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLDV

Sequences:

>Translated_473_residues
MAERLSNNFQFLDVPRHDPKKKDIDDRKHKYEEIYYPFETREVENQAHRCLHCGNPYCEWKCPVHNYIPNWLKLISEGNL
LEAVELSHQTNSLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGATEKYITDTALAMGWRPDMSKVVWTDKKVAVIGAGPA
GIGCADVLVRSGVKPVVFDRYEEIGGLLTFGIPEFKLEKPVMAKRREVFEGMGVEFRLGVEVGKDITIDELLADYDAVFM
GMGTYTYMKGGFPGEELDGVYEALPFLISNANRNLGFEKDEADFINMQGKRVVVLGGGDTAMDCNRTSIRQQAQSVSCAY
RRDEENMPGSRKEVTNAKEEGVQFLFNRQPIEVVGEDGKVVGVKVVETKLGEPDNNGRRRPEPIPGSEEILPADAVIVAF
GFRPSPADWFGEKSINTDDSGRVVAVEEQAFPFQTSNEKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLDV
>Mature_472_residues
AERLSNNFQFLDVPRHDPKKKDIDDRKHKYEEIYYPFETREVENQAHRCLHCGNPYCEWKCPVHNYIPNWLKLISEGNLL
EAVELSHQTNSLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGATEKYITDTALAMGWRPDMSKVVWTDKKVAVIGAGPAG
IGCADVLVRSGVKPVVFDRYEEIGGLLTFGIPEFKLEKPVMAKRREVFEGMGVEFRLGVEVGKDITIDELLADYDAVFMG
MGTYTYMKGGFPGEELDGVYEALPFLISNANRNLGFEKDEADFINMQGKRVVVLGGGDTAMDCNRTSIRQQAQSVSCAYR
RDEENMPGSRKEVTNAKEEGVQFLFNRQPIEVVGEDGKVVGVKVVETKLGEPDNNGRRRPEPIPGSEEILPADAVIVAFG
FRPSPADWFGEKSINTDDSGRVVAVEEQAFPFQTSNEKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLDV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=490, Percent_Identity=29.1836734693878, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=66.1702127659574, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=58.7852494577007, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI87082180, Length=461, Percent_Identity=54.2299349240781, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1788468, Length=454, Percent_Identity=28.8546255506608, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789243, Length=426, Percent_Identity=30.5164319248826, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=31.7813765182186, Blast_Score=222, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI71984108, Length=487, Percent_Identity=28.952772073922, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17543860, Length=251, Percent_Identity=26.2948207171315, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=500, Percent_Identity=31.2, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI28574881, Length=482, Percent_Identity=35.6846473029046, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24665539, Length=482, Percent_Identity=35.6846473029046, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=35.6846473029046, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=35.6846473029046, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24640763, Length=468, Percent_Identity=28.2051282051282, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18858217, Length=468, Percent_Identity=28.2051282051282, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52531; Mature: 52400

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLSNNFQFLDVPRHDPKKKDIDDRKHKYEEIYYPFETREVENQAHRCLHCGNPYCEW
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCEEE
KCPVHNYIPNWLKLISEGNLLEAVELSHQTNSLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGATEKYITDTALAMGWRPDMSKVVWTDKKVAVIGAGPAGIGCADVLVRSGVKPVVFDR
EECCCHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
YEEIGGLLTFGIPEFKLEKPVMAKRREVFEGMGVEFRLGVEVGKDITIDELLADYDAVFM
HHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
GMGTYTYMKGGFPGEELDGVYEALPFLISNANRNLGFEKDEADFINMQGKRVVVLGGGDT
CCCHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCC
AMDCNRTSIRQQAQSVSCAYRRDEENMPGSRKEVTNAKEEGVQFLFNRQPIEVVGEDGKV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCCCE
VGVKVVETKLGEPDNNGRRRPEPIPGSEEILPADAVIVAFGFRPSPADWFGEKSINTDDS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCC
GRVVAVEEQAFPFQTSNEKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLDV
CCEEEEECCCCCCCCCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
AERLSNNFQFLDVPRHDPKKKDIDDRKHKYEEIYYPFETREVENQAHRCLHCGNPYCEW
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCEEE
KCPVHNYIPNWLKLISEGNLLEAVELSHQTNSLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGATEKYITDTALAMGWRPDMSKVVWTDKKVAVIGAGPAGIGCADVLVRSGVKPVVFDR
EECCCHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
YEEIGGLLTFGIPEFKLEKPVMAKRREVFEGMGVEFRLGVEVGKDITIDELLADYDAVFM
HHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
GMGTYTYMKGGFPGEELDGVYEALPFLISNANRNLGFEKDEADFINMQGKRVVVLGGGDT
CCCHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCC
AMDCNRTSIRQQAQSVSCAYRRDEENMPGSRKEVTNAKEEGVQFLFNRQPIEVVGEDGKV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCCCE
VGVKVVETKLGEPDNNGRRRPEPIPGSEEILPADAVIVAFGFRPSPADWFGEKSINTDDS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCC
GRVVAVEEQAFPFQTSNEKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLDV
CCEEEEECCCCCCCCCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]