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The map label for this gene is bioB

Identifier: 110835081

GI number: 110835081

Start: 2533136

End: 2534287

Strand: Reverse

Name: bioB

Synonym: ABO_2220

Alternate gene names: 110835081

Gene position: 2534287-2533136 (Counterclockwise)

Preceding gene: 110835088

Following gene: 110835080

Centisome position: 81.22

GC content: 56.94

Gene sequence:

>1152_bases
ATGCCGGCTATCGCCAGTTCCCCCCAATCGCGATCCACGGATATGGCTGTACGCCATGACTGGAAACGTAGTGAGATCGA
CGCTCTGTTTGCTCTGCCATTCAACGACTTGTTGTTCCAGGCAGCCGCTTTGCACCGGGCCCATTTCGATCCTAATGCCG
TGCAGGTGAGCACCCTGTTGTCGATTAAGACCGGTGCTTGCCCGGAAGACTGCAAATACTGCTCCCAGTCTGGGCACTAC
AACACCGAGCTGGAAAAAGAAAAATTGTTGGAAGTTTCCAGAGTAGTGGAAGAGGCCCGTGCCGCTCGGCAAAACGGGGC
GTCCCGTTTCTGCATGGGCGCCGCTTGGCGTAGCCCCCGTGACAAGGATATGCCCTACGTGCTGGATATGATCCGTCAGG
TTAAAGACCTGGGCATGGAAACCTGCATGACCCTGGGTATGCTCAATGAAGGGCAGGCGGGAGCTCTCGCAGAGGCGGGC
CTGGATTACTACAACCACAATCTGGATACCTCCCCGGAATACTACGGGCAGGTGATCACCACCCGGACTTATAACGATCG
CCTTTCGACCTTAGCCAATGTGCGTGATGCGGGCATGAAAGTGTGCTGCGGTGGCATCGTAGGCATGGGCGAAGAGCGCA
ATGACCGGGTGGGTTTGTTGCAGCAGCTGGCGAATCTGCCGCACCATCCGGAATCGGTGCCGATCAATATGTTGGTTAAA
ATTGAAGGTACGCCGCTGGCGGATGTGGACGATCTGGACCCGTTTGAATTCGTGCGCACTGTAGCCGTGGCGCGAATTTT
GATGCCGCAATCCTATGTGCGGTTGTCCGCCGGCCGTCAGGAAATGAACGATGAAGCCCAGGCCCTGTGCTTTATGGCCG
GTGCCAATTCCATTTTTTATGGCGAACGCTTGCTGACTACCGATAACCCGGAAGCCAACCACGATCAGCGCTTGTTCAAG
CGCTTGGGTATCCATCCATTGGACCCCCGTCACGAGGCGTCGGACGAGGCCCATGAAGAAGCCATCCAGGCTCAGGTTTC
TGAGCAACAAGCCGAGGAGGCTGGGCTCTTCTACAATGCCATGGACAGCAAACGCACGGCCAAGCACGTGCTCGACAAGG
ACACCGGGCGCCCCACCGCTACCGAACACTGA

Upstream 100 bases:

>100_bases
GATTAAAAAATGTACTGCAGTAAACTTTTGTTGCGGCTTTCAGGTTGACAGGCATTACAGGGCTCCTTAGCCTGCTGAGG
TTAATCGAGGAGAATGAAGC

Downstream 100 bases:

>100_bases
GGTCTGCCCGTGGGCTTTGATCTGGCCACCGATCTGGCTGCACGCCGCCGCCAGCACCGTTACCGGCAAGCGCGTATCAT
GGATGGCCCCAGTGGCCGCC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPAIASSPQSRSTDMAVRHDWKRSEIDALFALPFNDLLFQAAALHRAHFDPNAVQVSTLLSIKTGACPEDCKYCSQSGHY
NTELEKEKLLEVSRVVEEARAARQNGASRFCMGAAWRSPRDKDMPYVLDMIRQVKDLGMETCMTLGMLNEGQAGALAEAG
LDYYNHNLDTSPEYYGQVITTRTYNDRLSTLANVRDAGMKVCCGGIVGMGEERNDRVGLLQQLANLPHHPESVPINMLVK
IEGTPLADVDDLDPFEFVRTVAVARILMPQSYVRLSAGRQEMNDEAQALCFMAGANSIFYGERLLTTDNPEANHDQRLFK
RLGIHPLDPRHEASDEAHEEAIQAQVSEQQAEEAGLFYNAMDSKRTAKHVLDKDTGRPTATEH

Sequences:

>Translated_383_residues
MPAIASSPQSRSTDMAVRHDWKRSEIDALFALPFNDLLFQAAALHRAHFDPNAVQVSTLLSIKTGACPEDCKYCSQSGHY
NTELEKEKLLEVSRVVEEARAARQNGASRFCMGAAWRSPRDKDMPYVLDMIRQVKDLGMETCMTLGMLNEGQAGALAEAG
LDYYNHNLDTSPEYYGQVITTRTYNDRLSTLANVRDAGMKVCCGGIVGMGEERNDRVGLLQQLANLPHHPESVPINMLVK
IEGTPLADVDDLDPFEFVRTVAVARILMPQSYVRLSAGRQEMNDEAQALCFMAGANSIFYGERLLTTDNPEANHDQRLFK
RLGIHPLDPRHEASDEAHEEAIQAQVSEQQAEEAGLFYNAMDSKRTAKHVLDKDTGRPTATEH
>Mature_382_residues
PAIASSPQSRSTDMAVRHDWKRSEIDALFALPFNDLLFQAAALHRAHFDPNAVQVSTLLSIKTGACPEDCKYCSQSGHYN
TELEKEKLLEVSRVVEEARAARQNGASRFCMGAAWRSPRDKDMPYVLDMIRQVKDLGMETCMTLGMLNEGQAGALAEAGL
DYYNHNLDTSPEYYGQVITTRTYNDRLSTLANVRDAGMKVCCGGIVGMGEERNDRVGLLQQLANLPHHPESVPINMLVKI
EGTPLADVDDLDPFEFVRTVAVARILMPQSYVRLSAGRQEMNDEAQALCFMAGANSIFYGERLLTTDNPEANHDQRLFKR
LGIHPLDPRHEASDEAHEEAIQAQVSEQQAEEAGLFYNAMDSKRTAKHVLDKDTGRPTATEH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=346, Percent_Identity=61.5606936416185, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=45.9119496855346, Blast_Score=325, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ALCBS (Q0VMD0)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693940.1
- ProteinModelPortal:   Q0VMD0
- SMR:   Q0VMD0
- STRING:   Q0VMD0
- GeneID:   4212134
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2220
- NMPDR:   fig|393595.12.peg.2219
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q0VMD0
- BioCyc:   ABOR393595:ABO_2220-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 42613; Mature: 42482

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIASSPQSRSTDMAVRHDWKRSEIDALFALPFNDLLFQAAALHRAHFDPNAVQVSTLL
CCCCCCCCCCCCCCHHHHCCCCHHHHCEEEECCHHHHHHHHHHHHHHCCCCCCEEHHHHH
SIKTGACPEDCKYCSQSGHYNTELEKEKLLEVSRVVEEARAARQNGASRFCMGAAWRSPR
HHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
DKDMPYVLDMIRQVKDLGMETCMTLGMLNEGQAGALAEAGLDYYNHNLDTSPEYYGQVIT
CCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCEEE
TRTYNDRLSTLANVRDAGMKVCCGGIVGMGEERNDRVGLLQQLANLPHHPESVPINMLVK
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEE
IEGTPLADVDDLDPFEFVRTVAVARILMPQSYVRLSAGRQEMNDEAQALCFMAGANSIFY
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCHHHHCHHHHHHHHHHCCCCEEE
GERLLTTDNPEANHDQRLFKRLGIHPLDPRHEASDEAHEEAIQAQVSEQQAEEAGLFYNA
CCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
MDSKRTAKHVLDKDTGRPTATEH
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PAIASSPQSRSTDMAVRHDWKRSEIDALFALPFNDLLFQAAALHRAHFDPNAVQVSTLL
CCCCCCCCCCCCCHHHHCCCCHHHHCEEEECCHHHHHHHHHHHHHHCCCCCCEEHHHHH
SIKTGACPEDCKYCSQSGHYNTELEKEKLLEVSRVVEEARAARQNGASRFCMGAAWRSPR
HHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
DKDMPYVLDMIRQVKDLGMETCMTLGMLNEGQAGALAEAGLDYYNHNLDTSPEYYGQVIT
CCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCEEE
TRTYNDRLSTLANVRDAGMKVCCGGIVGMGEERNDRVGLLQQLANLPHHPESVPINMLVK
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEE
IEGTPLADVDDLDPFEFVRTVAVARILMPQSYVRLSAGRQEMNDEAQALCFMAGANSIFY
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCHHHHCHHHHHHHHHHCCCCEEE
GERLLTTDNPEANHDQRLFKRLGIHPLDPRHEASDEAHEEAIQAQVSEQQAEEAGLFYNA
CCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
MDSKRTAKHVLDKDTGRPTATEH
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA