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The map label for this gene is miaA

Identifier: 110835065

GI number: 110835065

Start: 2515637

End: 2516626

Strand: Reverse

Name: miaA

Synonym: ABO_2204

Alternate gene names: 110835065

Gene position: 2516626-2515637 (Counterclockwise)

Preceding gene: 110835066

Following gene: 110835064

Centisome position: 80.66

GC content: 55.66

Gene sequence:

>990_bases
GTGATGCCTGAAGCGTCTCTGCCTATTTTATTGCTCATGGGGCCCACCTGCTCCGGTAAAACCGCGTTGGCGATTGAGGC
GGCGCAGACGCTACCCATCGAAATTATCAATGTGGATTCGGCGTTGGTGTATAAGGGCTTGAATATTGGTGCGGCGCGCC
CCACTGAAGAAGAGCTGGCATTGGCGCCACACCGGTTGCTGGGTTTTCGTGATCTTACCGAGCCCTATTCCGCTGCGGAT
TTTCGCGCCGATGCGCTCAAGCACATTGAACAGATCCACAACAACGGCAAATTGCCGTTGTTGGTGGGTGGCACGGTGCT
GTATTTCAAAGCCTTAGTGGAGGGGTTGGCTCCGTTGCCGGAAGCGGATCCGGTGGTGCGTGCCGAGATTGCAGCACTGG
CCGATGCGCAGGGCTGGCCGGCGGTGCATCAGGCGCTGGCTGAGGTGGACCCGGTCACCGCGGCGCGGCTCAAGCCCACG
GATAAGCAGCGGTTACAGAGGGCGCTAGAGGTCTTTCGTCTAACCGGCAGACCGTTGGCTGCGTTTCATGCGGAGCATGA
TAAAACGCTAACAAAGAGCCGGGATGGATTAAGTCAGTTCAGCGGATTGCCCTATCCTGTGCTCAGCACGGCCCTAGCGC
CGCAGGATAGAGCCTGGCTCCATGAACAGATTGCATTAAGGTTTCGCGCAATGCTGAATTCCGGTTTGCAGGAGGAAGTA
GAGGCGTTGCGACAGCAGCCCCAATTGAATGCAGATTTACCCGGTATTAGGGCCGTGGGATATCGCCAGATGTGGGAATA
TCTGGATGGTATGTACGGCTACGATAGCATGGTGGAGCGGGGCATTATTGCCACCCGGCAATTGGCTAAACGGCAGTTTA
CCTGGTTGAGGAAATGGCCGGAACTACAGTGGTTCGACAGTAATGATCGGTCACAACGAGACGCTCTGTTTGGATTGCTT
GCGAAGAACTGTAAATCTGTGTTTAGTTAG

Upstream 100 bases:

>100_bases
CTGGATCGACTGTTCATGCGGGGGCAATGAACCATGACCGCTTGCACGCGACACCCAAGCAGGCAACACGTGGAGGGCAC
GCGGTCTTTCGCGTGTCAGC

Downstream 100 bases:

>100_bases
CTCTGAAAGCATGCGTTTTAGTTTATAATGCAATGACTGCATAGGTCTGTGGCATTGCTGGGAAGGTGTCGCGGCTATGG
ATGGGGCGGGATTCGGAACC

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MMPEASLPILLLMGPTCSGKTALAIEAAQTLPIEIINVDSALVYKGLNIGAARPTEEELALAPHRLLGFRDLTEPYSAAD
FRADALKHIEQIHNNGKLPLLVGGTVLYFKALVEGLAPLPEADPVVRAEIAALADAQGWPAVHQALAEVDPVTAARLKPT
DKQRLQRALEVFRLTGRPLAAFHAEHDKTLTKSRDGLSQFSGLPYPVLSTALAPQDRAWLHEQIALRFRAMLNSGLQEEV
EALRQQPQLNADLPGIRAVGYRQMWEYLDGMYGYDSMVERGIIATRQLAKRQFTWLRKWPELQWFDSNDRSQRDALFGLL
AKNCKSVFS

Sequences:

>Translated_329_residues
MMPEASLPILLLMGPTCSGKTALAIEAAQTLPIEIINVDSALVYKGLNIGAARPTEEELALAPHRLLGFRDLTEPYSAAD
FRADALKHIEQIHNNGKLPLLVGGTVLYFKALVEGLAPLPEADPVVRAEIAALADAQGWPAVHQALAEVDPVTAARLKPT
DKQRLQRALEVFRLTGRPLAAFHAEHDKTLTKSRDGLSQFSGLPYPVLSTALAPQDRAWLHEQIALRFRAMLNSGLQEEV
EALRQQPQLNADLPGIRAVGYRQMWEYLDGMYGYDSMVERGIIATRQLAKRQFTWLRKWPELQWFDSNDRSQRDALFGLL
AKNCKSVFS
>Mature_329_residues
MMPEASLPILLLMGPTCSGKTALAIEAAQTLPIEIINVDSALVYKGLNIGAARPTEEELALAPHRLLGFRDLTEPYSAAD
FRADALKHIEQIHNNGKLPLLVGGTVLYFKALVEGLAPLPEADPVVRAEIAALADAQGWPAVHQALAEVDPVTAARLKPT
DKQRLQRALEVFRLTGRPLAAFHAEHDKTLTKSRDGLSQFSGLPYPVLSTALAPQDRAWLHEQIALRFRAMLNSGLQEEV
EALRQQPQLNADLPGIRAVGYRQMWEYLDGMYGYDSMVERGIIATRQLAKRQFTWLRKWPELQWFDSNDRSQRDALFGLL
AKNCKSVFS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=30.9904153354633, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1790613, Length=324, Percent_Identity=55.5555555555556, Blast_Score=333, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI25144712, Length=184, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=327, Percent_Identity=26.9113149847095, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24649830, Length=362, Percent_Identity=25.9668508287293, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ALCBS (Q0VME6)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693924.1
- ProteinModelPortal:   Q0VME6
- SMR:   Q0VME6
- STRING:   Q0VME6
- GeneID:   4211582
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2204
- NMPDR:   fig|393595.12.peg.2203
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q0VME6
- BioCyc:   ABOR393595:ABO_2204-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36537; Mature: 36537

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPEASLPILLLMGPTCSGKTALAIEAAQTLPIEIINVDSALVYKGLNIGAARPTEEELA
CCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHH
LAPHRLLGFRDLTEPYSAADFRADALKHIEQIHNNGKLPLLVGGTVLYFKALVEGLAPLP
HCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCC
EADPVVRAEIAALADAQGWPAVHQALAEVDPVTAARLKPTDKQRLQRALEVFRLTGRPLA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHH
AFHAEHDKTLTKSRDGLSQFSGLPYPVLSTALAPQDRAWLHEQIALRFRAMLNSGLQEEV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
EALRQQPQLNADLPGIRAVGYRQMWEYLDGMYGYDSMVERGIIATRQLAKRQFTWLRKWP
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELQWFDSNDRSQRDALFGLLAKNCKSVFS
CCEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMPEASLPILLLMGPTCSGKTALAIEAAQTLPIEIINVDSALVYKGLNIGAARPTEEELA
CCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHH
LAPHRLLGFRDLTEPYSAADFRADALKHIEQIHNNGKLPLLVGGTVLYFKALVEGLAPLP
HCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCC
EADPVVRAEIAALADAQGWPAVHQALAEVDPVTAARLKPTDKQRLQRALEVFRLTGRPLA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHH
AFHAEHDKTLTKSRDGLSQFSGLPYPVLSTALAPQDRAWLHEQIALRFRAMLNSGLQEEV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
EALRQQPQLNADLPGIRAVGYRQMWEYLDGMYGYDSMVERGIIATRQLAKRQFTWLRKWP
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELQWFDSNDRSQRDALFGLLAKNCKSVFS
CCEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA