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The map label for this gene is hisZ [H]

Identifier: 110835059

GI number: 110835059

Start: 2509564

End: 2510739

Strand: Reverse

Name: hisZ [H]

Synonym: ABO_2198

Alternate gene names: 110835059

Gene position: 2510739-2509564 (Counterclockwise)

Preceding gene: 110835060

Following gene: 110835058

Centisome position: 80.47

GC content: 60.29

Gene sequence:

>1176_bases
ATGATGAATCAGGAAGAACAGTGGCTACTGCCCGACGGGGTAGAAGAGGTCCTCCCGGGTCGCGCCCGGGCCATCGAGCA
GCTGCGTCGGCAGACGCTGGATCTGTATCAGCGTTGGGGTTACGAACTTGTTTTCCCGCCGTTGATTGAGTTTATGGAAT
CCTTGCTGAACGGCGGTGGCCGTGATCTGGAGAGGGAAACCTTCAAGATTACCGACCAGCTCACCGGGCGCCTGATGGGT
GTTCGTGCGGATATTACCCCGCAAGTGGCGCGTATGGATGCCCATAGCTTGCGCCGTAACGCACCTTCTCGTCTTTGTTA
CTGCTCCAGCGCCCTGCGCACCCGGCCGGTGGTCGCCGGTGGCACGCGCCTGCCCTATCAGTTGGGCGTAGAGCTATTTG
GTCACGCCGGTTCAGACAGTGATCTCGAGGTGATTTCCTTGCTGCTGGAAACGCTGGCATTGTCAGGCGTCGAGCAACAG
GTGGTACTGGATTTGGGCCACGTGGGTTTGTTCCGTGGTCTGCTCGAGGGCTGTGATTTGAGCGAAACTGAGCAGAACCA
ATTGGAAGATATCTATGTGCGCAAAGCGCGGGTAGAGCTGGATTCTTTTATTGCCAGCCATGATTTGCCTGCGCCTGCGG
CCAAGGCCTTGGCCGCCTTGCCTTGGTTAGCAGGTGGCGTGGAGGTGCTCAATGAGGCCCGTGCGTTATTGTCCCACTTC
TCTGGCGCCCTAGCGGCATTGGATGAGCTGGCGGTGCTGGCCGATGTGCTCGGCGCTCGAGGGGTGCGGCTGCACCTGGA
CTTGGGTGAGCTGCGTGGTTATCACTACCACACCGGTTGCGTGTTTGCGGCGTACTTGCCCGGTGAGAGCGAGCCCGTGG
CCAAGGGCGGTCGCTACGATCACATTGGTGAAGTATTTGGTCGGGCCCGCCCGGCCACCGGCTTCAGTGCGGATTTGAAG
ATGCTGGCTGAGCGTGCGCAATCTGCCGCGCTTACCGGTATTCTGGCTGAAGGGTCGCTGCAGGACGCCGCGTTCGCGAC
TAGGGTTGCGGCTCTTCGTGAGCAGGGCGAGCGTGTGGTGCTGGCCCTTCCCGGTGCAGATAATGATCCGGCTGAACTAG
GGTGCCAGCGTCGTCTGGTTGCTCAGGCCGATGGTTGGGTTGTGACCGACCTCTAA

Upstream 100 bases:

>100_bases
GGCCCTTCAGGTGGCGCGTCTGGATAGTCGCCTGCTGCGAATTATAGGGCTGGCACTCATGGTATTGGGTGCGGGCTTGT
TATTACTGATGCGGTCGTAA

Downstream 100 bases:

>100_bases
AACCGAATTTCTTTTTTCCTGAATAGATGGCAGCAAGCAATCATGGGTAAGAACGTAGTCATTCTCGGCACCCAGTGGGG
TGATGAAGGCAAAGGCAAAA

Product: ATP phosphoribosyl transferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MMNQEEQWLLPDGVEEVLPGRARAIEQLRRQTLDLYQRWGYELVFPPLIEFMESLLNGGGRDLERETFKITDQLTGRLMG
VRADITPQVARMDAHSLRRNAPSRLCYCSSALRTRPVVAGGTRLPYQLGVELFGHAGSDSDLEVISLLLETLALSGVEQQ
VVLDLGHVGLFRGLLEGCDLSETEQNQLEDIYVRKARVELDSFIASHDLPAPAAKALAALPWLAGGVEVLNEARALLSHF
SGALAALDELAVLADVLGARGVRLHLDLGELRGYHYHTGCVFAAYLPGESEPVAKGGRYDHIGEVFGRARPATGFSADLK
MLAERAQSAALTGILAEGSLQDAAFATRVAALREQGERVVLALPGADNDPAELGCQRRLVAQADGWVVTDL

Sequences:

>Translated_391_residues
MMNQEEQWLLPDGVEEVLPGRARAIEQLRRQTLDLYQRWGYELVFPPLIEFMESLLNGGGRDLERETFKITDQLTGRLMG
VRADITPQVARMDAHSLRRNAPSRLCYCSSALRTRPVVAGGTRLPYQLGVELFGHAGSDSDLEVISLLLETLALSGVEQQ
VVLDLGHVGLFRGLLEGCDLSETEQNQLEDIYVRKARVELDSFIASHDLPAPAAKALAALPWLAGGVEVLNEARALLSHF
SGALAALDELAVLADVLGARGVRLHLDLGELRGYHYHTGCVFAAYLPGESEPVAKGGRYDHIGEVFGRARPATGFSADLK
MLAERAQSAALTGILAEGSLQDAAFATRVAALREQGERVVLALPGADNDPAELGCQRRLVAQADGWVVTDL
>Mature_391_residues
MMNQEEQWLLPDGVEEVLPGRARAIEQLRRQTLDLYQRWGYELVFPPLIEFMESLLNGGGRDLERETFKITDQLTGRLMG
VRADITPQVARMDAHSLRRNAPSRLCYCSSALRTRPVVAGGTRLPYQLGVELFGHAGSDSDLEVISLLLETLALSGVEQQ
VVLDLGHVGLFRGLLEGCDLSETEQNQLEDIYVRKARVELDSFIASHDLPAPAAKALAALPWLAGGVEVLNEARALLSHF
SGALAALDELAVLADVLGARGVRLHLDLGELRGYHYHTGCVFAAYLPGESEPVAKGGRYDHIGEVFGRARPATGFSADLK
MLAERAQSAALTGILAEGSLQDAAFATRVAALREQGERVVLALPGADNDPAELGCQRRLVAQADGWVVTDL

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=326, Percent_Identity=26.0736196319018, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 42452; Mature: 42452

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNQEEQWLLPDGVEEVLPGRARAIEQLRRQTLDLYQRWGYELVFPPLIEFMESLLNGGG
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCC
RDLERETFKITDQLTGRLMGVRADITPQVARMDAHSLRRNAPSRLCYCSSALRTRPVVAG
CCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEC
GTRLPYQLGVELFGHAGSDSDLEVISLLLETLALSGVEQQVVLDLGHVGLFRGLLEGCDL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
SETEQNQLEDIYVRKARVELDSFIASHDLPAPAAKALAALPWLAGGVEVLNEARALLSHF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGALAALDELAVLADVLGARGVRLHLDLGELRGYHYHTGCVFAAYLPGESEPVAKGGRYD
HHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCEEECCEEEEEECCCCCCCCCCCCCCH
HIGEVFGRARPATGFSADLKMLAERAQSAALTGILAEGSLQDAAFATRVAALREQGERVV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
LALPGADNDPAELGCQRRLVAQADGWVVTDL
EECCCCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MMNQEEQWLLPDGVEEVLPGRARAIEQLRRQTLDLYQRWGYELVFPPLIEFMESLLNGGG
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCC
RDLERETFKITDQLTGRLMGVRADITPQVARMDAHSLRRNAPSRLCYCSSALRTRPVVAG
CCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEC
GTRLPYQLGVELFGHAGSDSDLEVISLLLETLALSGVEQQVVLDLGHVGLFRGLLEGCDL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
SETEQNQLEDIYVRKARVELDSFIASHDLPAPAAKALAALPWLAGGVEVLNEARALLSHF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGALAALDELAVLADVLGARGVRLHLDLGELRGYHYHTGCVFAAYLPGESEPVAKGGRYD
HHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCEEECCEEEEEECCCCCCCCCCCCCCH
HIGEVFGRARPATGFSADLKMLAERAQSAALTGILAEGSLQDAAFATRVAALREQGERVV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
LALPGADNDPAELGCQRRLVAQADGWVVTDL
EECCCCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA