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The map label for this gene is purA

Identifier: 110835058

GI number: 110835058

Start: 2508229

End: 2509521

Strand: Reverse

Name: purA

Synonym: ABO_2197

Alternate gene names: 110835058

Gene position: 2509521-2508229 (Counterclockwise)

Preceding gene: 110835059

Following gene: 110835057

Centisome position: 80.43

GC content: 58.08

Gene sequence:

>1293_bases
ATGGGTAAGAACGTAGTCATTCTCGGCACCCAGTGGGGTGATGAAGGCAAAGGCAAAATCGTCGATCTGCTTACCGATCA
GGCCTCATTGGTCGCCCGCTTTCAGGGTGGCCATAACGCTGGCCATACCCTGGTGATCGATGGCAAGAAAACGGTCCTGC
ACCTGATTCCGTCAGGCATTCTTCGTGAAGATGTGCAGTGCCTGATCGGTAACGGCGTGGTGCTTTCTCTGGAAGCCTTG
CTTAAGGAAATTGGTGGCCTGGAAGATCAGGGTGTGCCGGTTCGCGATCGCCTGCGTTTGTCCGCCTCCTGCCCGCTGAT
CTTGCCTGTGCACGTGGCTCTGGATCAGGCTCGGGAAGCGGCCCGTGGCAACAAGAAAATAGGCACCACGGGCCGGGGCA
TCGGCCCGGCCTATGAAGACAAGGTGGCTCGTCGAGGCTTGCGCCTGGGTGATGTGTACCAGCGTGAGCTGTTTGCCGCC
AAGCTGGGCGAAGTGATGGATTACCACAACTTTGTTCTGCGCAGCTATTACAATGCGGAGCCGGTGGATTTCCAGAAAAC
CCTGGATGACACCTTGCAGCTGGGCGAAGACGTTCGCAGCATGGTGACTGACGTGACGGCGGTGCTGCACGGCGCCCGTG
AGCGCGGCGAAAATATCATGTTCGAAGGGGCCCAGGGCACCCTGCTGGATATCGACCACGGCACCTACCCTTATGTCACC
AGCTCCAATACCACCGCTGGCGGTACCGCTACCGGCACCGGGTTTGGTCCTCTGTACCTAGATTATGTGCTGGGTATTAC
CAAGGCCTACACCACCCGGGTGGGGAGTGGCCCGTTCCCCACTGAGTTGTTCGACGAGAATGGCCGCTACCTGGCCGAGA
AGGGGCACGAATTTGGTTCTACCACGGGCCGTGCTCGCCGTTGCGGGTGGTTCGATGCGGTGGCGCTGAAGCGCTCCATC
CAGATTAACTCCATTACAGGCCTGTGCCTAACCAAGCTGGATGTGCTGGATGGCCTGGAAGAGATCAACATCTGTGTGGG
CTACCAGGATGCGGCCGGCAACTCGCTGGAGTGTGCCTGGGATGCGGATAGCTACGAAGAAGTGAAGCCAGTGTATGAAA
GCCTGCCGGGCTGGAGCGAATCTACCTTGGGGGTGAAGTCAGAAGATGATTTGCCGCCCAACGCCAAGGCTTACCTGAAG
CGTATTGAAGCAATTACCGGTGCGCCCATCGATATTATTTCCACAGGCCCGGACCGTGTGGAAACCATTATCAAGCGCCA
TCCGTTCTCCTGA

Upstream 100 bases:

>100_bases
AGGGTGCCAGCGTCGTCTGGTTGCTCAGGCCGATGGTTGGGTTGTGACCGACCTCTAAAACCGAATTTCTTTTTTCCTGA
ATAGATGGCAGCAAGCAATC

Downstream 100 bases:

>100_bases
ACCCGGCGCAAATGGAAAAGCGGCCCAAGGGCCGCTTTTTTTATGTCCGACGCTACGGTTTTTGTGTTGAACAAATTCTT
CTTATCTCCGTGACAGATTT

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGKNVVILGTQWGDEGKGKIVDLLTDQASLVARFQGGHNAGHTLVIDGKKTVLHLIPSGILREDVQCLIGNGVVLSLEAL
LKEIGGLEDQGVPVRDRLRLSASCPLILPVHVALDQAREAARGNKKIGTTGRGIGPAYEDKVARRGLRLGDVYQRELFAA
KLGEVMDYHNFVLRSYYNAEPVDFQKTLDDTLQLGEDVRSMVTDVTAVLHGARERGENIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGTATGTGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDENGRYLAEKGHEFGSTTGRARRCGWFDAVALKRSI
QINSITGLCLTKLDVLDGLEEINICVGYQDAAGNSLECAWDADSYEEVKPVYESLPGWSESTLGVKSEDDLPPNAKAYLK
RIEAITGAPIDIISTGPDRVETIIKRHPFS

Sequences:

>Translated_430_residues
MGKNVVILGTQWGDEGKGKIVDLLTDQASLVARFQGGHNAGHTLVIDGKKTVLHLIPSGILREDVQCLIGNGVVLSLEAL
LKEIGGLEDQGVPVRDRLRLSASCPLILPVHVALDQAREAARGNKKIGTTGRGIGPAYEDKVARRGLRLGDVYQRELFAA
KLGEVMDYHNFVLRSYYNAEPVDFQKTLDDTLQLGEDVRSMVTDVTAVLHGARERGENIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGTATGTGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDENGRYLAEKGHEFGSTTGRARRCGWFDAVALKRSI
QINSITGLCLTKLDVLDGLEEINICVGYQDAAGNSLECAWDADSYEEVKPVYESLPGWSESTLGVKSEDDLPPNAKAYLK
RIEAITGAPIDIISTGPDRVETIIKRHPFS
>Mature_429_residues
GKNVVILGTQWGDEGKGKIVDLLTDQASLVARFQGGHNAGHTLVIDGKKTVLHLIPSGILREDVQCLIGNGVVLSLEALL
KEIGGLEDQGVPVRDRLRLSASCPLILPVHVALDQAREAARGNKKIGTTGRGIGPAYEDKVARRGLRLGDVYQRELFAAK
LGEVMDYHNFVLRSYYNAEPVDFQKTLDDTLQLGEDVRSMVTDVTAVLHGARERGENIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGTATGTGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDENGRYLAEKGHEFGSTTGRARRCGWFDAVALKRSIQ
INSITGLCLTKLDVLDGLEEINICVGYQDAAGNSLECAWDADSYEEVKPVYESLPGWSESTLGVKSEDDLPPNAKAYLKR
IEAITGAPIDIISTGPDRVETIIKRHPFS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=41.7249417249417, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=41.0672853828306, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=40, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=68.1395348837209, Blast_Score=613, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=41.5313225058005, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=41.5313225058005, Blast_Score=321, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=426, Percent_Identity=44.3661971830986, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=41.8823529411765, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ALCBS (Q0VMF3)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693917.1
- ProteinModelPortal:   Q0VMF3
- SMR:   Q0VMF3
- STRING:   Q0VMF3
- GeneID:   4211140
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2197
- NMPDR:   fig|393595.12.peg.2197
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q0VMF3
- BioCyc:   ABOR393595:ABO_2197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46611; Mature: 46480

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVILGTQWGDEGKGKIVDLLTDQASLVARFQGGHNAGHTLVIDGKKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEHHCHHHHHHHHCCCCCCCCEEEECCCHHEEHHHCCHH
LREDVQCLIGNGVVLSLEALLKEIGGLEDQGVPVRDRLRLSASCPLILPVHVALDQAREA
HHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHHHEECCCCCEEEEHHHHHHHHHHH
ARGNKKIGTTGRGIGPAYEDKVARRGLRLGDVYQRELFAAKLGEVMDYHNFVLRSYYNAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVDFQKTLDDTLQLGEDVRSMVTDVTAVLHGARERGENIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGTATGTGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDENGRYLAEKGHEFGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEHHCCCCCCC
TTGRARRCGWFDAVALKRSIQINSITGLCLTKLDVLDGLEEINICVGYQDAAGNSLECAW
CCCCCHHCCHHHHHHHHHHEEEECCHHHHHHHHHHHCCHHHCEEEEEECCCCCCCEEEEE
DADSYEEVKPVYESLPGWSESTLGVKSEDDLPPNAKAYLKRIEAITGAPIDIISTGPDRV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHH
ETIIKRHPFS
HHHHHHCCCC
>Mature Secondary Structure 
GKNVVILGTQWGDEGKGKIVDLLTDQASLVARFQGGHNAGHTLVIDGKKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEHHCHHHHHHHHCCCCCCCCEEEECCCHHEEHHHCCHH
LREDVQCLIGNGVVLSLEALLKEIGGLEDQGVPVRDRLRLSASCPLILPVHVALDQAREA
HHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHHHEECCCCCEEEEHHHHHHHHHHH
ARGNKKIGTTGRGIGPAYEDKVARRGLRLGDVYQRELFAAKLGEVMDYHNFVLRSYYNAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVDFQKTLDDTLQLGEDVRSMVTDVTAVLHGARERGENIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGTATGTGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDENGRYLAEKGHEFGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEHHCCCCCCC
TTGRARRCGWFDAVALKRSIQINSITGLCLTKLDVLDGLEEINICVGYQDAAGNSLECAW
CCCCCHHCCHHHHHHHHHHEEEECCHHHHHHHHHHHCCHHHCEEEEEECCCCCCCEEEEE
DADSYEEVKPVYESLPGWSESTLGVKSEDDLPPNAKAYLKRIEAITGAPIDIISTGPDRV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHH
ETIIKRHPFS
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA