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The map label for this gene is yjfh [H]

Identifier: 110835053

GI number: 110835053

Start: 2500562

End: 2501317

Strand: Reverse

Name: yjfh [H]

Synonym: ABO_2192

Alternate gene names: 110835053

Gene position: 2501317-2500562 (Counterclockwise)

Preceding gene: 110835054

Following gene: 110835052

Centisome position: 80.17

GC content: 60.85

Gene sequence:

>756_bases
ATGAATAAACCCCTAATGTATTCCGGCTTCCATGCGGTGGAGGCGCTGTTGCGCCATCGGCCGGAGGCAGTGCTGGAGCT
GTTCGTTCAGGATACCCGGGCCGACCGGGATGATCCGCGTCTGGCTGCTCTGATGCAGGCAGCAAAAGATTTCGGTATCG
CTATCCAGCGAGCTCGGCGCGAGGTGCTTGAAAAGCACGCAGGGCCCCAGAATCAGGGCATCGTGGCGCGGGCGCGACCC
CGTCGCCCTTTAGATGAGAGCGCGTTGTTGCAGTGGCTGGACGGCAAGCCGGCCAAGCCGTTGCTGCTGGTTCTGGAAAA
CGTTACCGATCCGCATAATCTCGGCGCCTGCCTGCGCAGTGCGGATGCTGCCGGGGTTCAGGCAGTGATTGTGCCGCGCC
GTAATGCGGCAGGCCTGACGCCGGTGGCATGTCGCACTGCGGTTGGGGCGGCGGAGAGCCTGGCGTATTACGAGATTGGT
AATTTGGCTAGCCTGCTGGACCAGCTGCGCGATCGAGGGGTTTGGGTGGTTGGCACGGCTTTAGAAGAACGAAGTGAGTC
CCTGTTTACCTTCCAGGCCCCGGAATCCCTGGCCGTGGTGATGGGGGCTGAAGGGAGTGGGTTAAAGCGCCTGACCCGTG
ATAAGTGCGACCAGTTGTTGGAGATACCCATGGCTGGGGAAGTGCAGAGCCTCAATGTGTCTGTTGCTACTGGGGTTATG
CTGTTTCAGGTGCGGGCTCAACGCAGCGTGGAATGA

Upstream 100 bases:

>100_bases
ATGGCATTAATGCGCGGCTTAAGCGCTGCTCCTGCGTTTTGATTCCATTTTCACTGCGTATCGCGTGCTTGCGTGGTGCG
TTTAGCGTGCAGGCGTCGTT

Downstream 100 bases:

>100_bases
CGCAGTAAGTTGCTGCCGGCCACGCTCCGGCGGCGCGCCCTTGCGACTCTCAACCCTTTTTTATTGCCGCCGCCTCGCTC
GAGTGTGCATGGTCGTGTTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNKPLMYSGFHAVEALLRHRPEAVLELFVQDTRADRDDPRLAALMQAAKDFGIAIQRARREVLEKHAGPQNQGIVARARP
RRPLDESALLQWLDGKPAKPLLLVLENVTDPHNLGACLRSADAAGVQAVIVPRRNAAGLTPVACRTAVGAAESLAYYEIG
NLASLLDQLRDRGVWVVGTALEERSESLFTFQAPESLAVVMGAEGSGLKRLTRDKCDQLLEIPMAGEVQSLNVSVATGVM
LFQVRAQRSVE

Sequences:

>Translated_251_residues
MNKPLMYSGFHAVEALLRHRPEAVLELFVQDTRADRDDPRLAALMQAAKDFGIAIQRARREVLEKHAGPQNQGIVARARP
RRPLDESALLQWLDGKPAKPLLLVLENVTDPHNLGACLRSADAAGVQAVIVPRRNAAGLTPVACRTAVGAAESLAYYEIG
NLASLLDQLRDRGVWVVGTALEERSESLFTFQAPESLAVVMGAEGSGLKRLTRDKCDQLLEIPMAGEVQSLNVSVATGVM
LFQVRAQRSVE
>Mature_251_residues
MNKPLMYSGFHAVEALLRHRPEAVLELFVQDTRADRDDPRLAALMQAAKDFGIAIQRARREVLEKHAGPQNQGIVARARP
RRPLDESALLQWLDGKPAKPLLLVLENVTDPHNLGACLRSADAAGVQAVIVPRRNAAGLTPVACRTAVGAAESLAYYEIG
NLASLLDQLRDRGVWVVGTALEERSESLFTFQAPESLAVVMGAEGSGLKRLTRDKCDQLLEIPMAGEVQSLNVSVATGVM
LFQVRAQRSVE

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=241, Percent_Identity=32.3651452282158, Blast_Score=98, Evalue=6e-21,
Organism=Escherichia coli, GI1790623, Length=248, Percent_Identity=49.1935483870968, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1788935, Length=235, Percent_Identity=33.6170212765957, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790083, Length=151, Percent_Identity=30.4635761589404, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=255, Percent_Identity=27.843137254902, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27321; Mature: 27321

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPLMYSGFHAVEALLRHRPEAVLELFVQDTRADRDDPRLAALMQAAKDFGIAIQRARR
CCCCCEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLEKHAGPQNQGIVARARPRRPLDESALLQWLDGKPAKPLLLVLENVTDPHNLGACLRS
HHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHC
ADAAGVQAVIVPRRNAAGLTPVACRTAVGAAESLAYYEIGNLASLLDQLRDRGVWVVGTA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHH
LEERSESLFTFQAPESLAVVMGAEGSGLKRLTRDKCDQLLEIPMAGEVQSLNVSVATGVM
HHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECEEHHHHHHH
LFQVRAQRSVE
HHHHHHHHCCC
>Mature Secondary Structure
MNKPLMYSGFHAVEALLRHRPEAVLELFVQDTRADRDDPRLAALMQAAKDFGIAIQRARR
CCCCCEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLEKHAGPQNQGIVARARPRRPLDESALLQWLDGKPAKPLLLVLENVTDPHNLGACLRS
HHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHC
ADAAGVQAVIVPRRNAAGLTPVACRTAVGAAESLAYYEIGNLASLLDQLRDRGVWVVGTA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHH
LEERSESLFTFQAPESLAVVMGAEGSGLKRLTRDKCDQLLEIPMAGEVQSLNVSVATGVM
HHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECEEHHHHHHH
LFQVRAQRSVE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]