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The map label for this gene is dusC [H]

Identifier: 110835023

GI number: 110835023

Start: 2470692

End: 2471636

Strand: Reverse

Name: dusC [H]

Synonym: ABO_2162

Alternate gene names: 110835023

Gene position: 2471636-2470692 (Counterclockwise)

Preceding gene: 110835028

Following gene: 110835020

Centisome position: 79.22

GC content: 59.47

Gene sequence:

>945_bases
ATGCGCGCCATGAAATTGATTCTGGCTCCGATGGAAGGCCTGGCGGATTTTTGGGTGCGCCAGGCGCTGACCGATGTGGG
CGACTATGACTGGTGTGTTAGTGAGTTTGTTCGCGTTAGCGGGCTACTGCTGCCGCCCAAAGTATTTTACCGCTGGTGCC
CGGAGCTAAAACTCGGTGCGCGTACCCGGGCGGGCACCCCTGTGCACCTTCAGTTGCTCGGTTCCGACCCGGCCTGCATG
GCGGATAATGCGGAGCGAGCAGTAGAGCTGGGCGCACCCGCCATTGATCTGAACTTCGGCTGTCCGGCAAAAACGGTGAA
TCGACATCGTGGTGGTGCGGTGCTGCTTGATGAGCCGGACGTGGTGCATGCGGTGACGGCGGCAGTGCGCCAGGCGGTCC
CGTCCCAGGTGCCGGTGTCAGTAAAGATGCGTCTGGGTAATGAAGATGACGCTCGTGCCTTGGATAATGCCAGGGCCGTG
GTGGACGCTGGGGCGGCTTGGCTGACGGTGCATGGGCGCACCAAACGTCAAGGGTATAAGCCGCCGGCGTTTTGGGACCG
TATCGGCCACATTCGTGAGGTAGTGTCGATTCCGGTAATTGCCAATGGCGAAATTTGGACACCGGACGACGCTGGCCGAG
CCCAGCGGGAAAGTGGTTGTGAGGATCTTATGTTGGGTCGAGGGGCGGTGGCCAATCCATTGCTAGTTTCGTTGTTGCGG
TTCGGCGGCGAAACCAATTGGGATGCGATCTTGCCTATATTGCAACAATTCTGGATGGACGTGGCTGATACCGGCACTGA
TGCCCAGTTAGCTGGGCGGATCAAGCAGTGGCTAAGCTACCTGCGCCGCCGTTGGCCGCAGGCGGCGCAATTGCTGGAAC
AGGTGAAACGAGAAACGAGCCCGGGGGTTATTGGTCGATTGTTGGCGGATTTTGAGCCGCAGTAA

Upstream 100 bases:

>100_bases
ACCGGGGGTTTTGTGTTGTGCCTCATTGTGTAGGGAGCCGTGTGCTGAAGGTTTTCCCGTTCAGATTTATTCGTATTCGG
TGGCGCGGCAGTAGCCTAGA

Downstream 100 bases:

>100_bases
CCGTTAGCGTAAAAAATTGAAAAGGGAGGCACGACGTCGCCCCCCCCCTATGGCGATCCGCCAGCGGGTGAAATGATTGT
GCAAATTATCTAACCCCCAG

Product: dihydrouridine synthase (Dus) superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRAMKLILAPMEGLADFWVRQALTDVGDYDWCVSEFVRVSGLLLPPKVFYRWCPELKLGARTRAGTPVHLQLLGSDPACM
ADNAERAVELGAPAIDLNFGCPAKTVNRHRGGAVLLDEPDVVHAVTAAVRQAVPSQVPVSVKMRLGNEDDARALDNARAV
VDAGAAWLTVHGRTKRQGYKPPAFWDRIGHIREVVSIPVIANGEIWTPDDAGRAQRESGCEDLMLGRGAVANPLLVSLLR
FGGETNWDAILPILQQFWMDVADTGTDAQLAGRIKQWLSYLRRRWPQAAQLLEQVKRETSPGVIGRLLADFEPQ

Sequences:

>Translated_314_residues
MRAMKLILAPMEGLADFWVRQALTDVGDYDWCVSEFVRVSGLLLPPKVFYRWCPELKLGARTRAGTPVHLQLLGSDPACM
ADNAERAVELGAPAIDLNFGCPAKTVNRHRGGAVLLDEPDVVHAVTAAVRQAVPSQVPVSVKMRLGNEDDARALDNARAV
VDAGAAWLTVHGRTKRQGYKPPAFWDRIGHIREVVSIPVIANGEIWTPDDAGRAQRESGCEDLMLGRGAVANPLLVSLLR
FGGETNWDAILPILQQFWMDVADTGTDAQLAGRIKQWLSYLRRRWPQAAQLLEQVKRETSPGVIGRLLADFEPQ
>Mature_314_residues
MRAMKLILAPMEGLADFWVRQALTDVGDYDWCVSEFVRVSGLLLPPKVFYRWCPELKLGARTRAGTPVHLQLLGSDPACM
ADNAERAVELGAPAIDLNFGCPAKTVNRHRGGAVLLDEPDVVHAVTAAVRQAVPSQVPVSVKMRLGNEDDARALDNARAV
VDAGAAWLTVHGRTKRQGYKPPAFWDRIGHIREVVSIPVIANGEIWTPDDAGRAQRESGCEDLMLGRGAVANPLLVSLLR
FGGETNWDAILPILQQFWMDVADTGTDAQLAGRIKQWLSYLRRRWPQAAQLLEQVKRETSPGVIGRLLADFEPQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=172, Percent_Identity=34.8837209302326, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI40807366, Length=175, Percent_Identity=36, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI239788483, Length=320, Percent_Identity=28.75, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI239788462, Length=320, Percent_Identity=28.75, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788462, Length=295, Percent_Identity=47.4576271186441, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789660, Length=230, Percent_Identity=38.2608695652174, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693211, Length=208, Percent_Identity=29.3269230769231, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=37.6623376623377, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=29.4930875576037, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=239, Percent_Identity=24.6861924686192, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=205, Percent_Identity=29.7560975609756, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=173, Percent_Identity=32.9479768786127, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=29.3388429752066, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=29.3388429752066, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24585320, Length=304, Percent_Identity=26.6447368421053, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34554; Mature: 34554

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMKLILAPMEGLADFWVRQALTDVGDYDWCVSEFVRVSGLLLPPKVFYRWCPELKLGA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
RTRAGTPVHLQLLGSDPACMADNAERAVELGAPAIDLNFGCPAKTVNRHRGGAVLLDEPD
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEECCCCCCHHHHHCCCCCEEEECCCH
VVHAVTAAVRQAVPSQVPVSVKMRLGNEDDARALDNARAVVDAGAAWLTVHGRTKRQGYK
HHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCC
PPAFWDRIGHIREVVSIPVIANGEIWTPDDAGRAQRESGCEDLMLGRGAVANPLLVSLLR
CCHHHHHHHHHHHHHHCCEEECCCEECCCCCCHHHHHCCCHHHHHCCCCCCHHHHHHHHH
FGGETNWDAILPILQQFWMDVADTGTDAQLAGRIKQWLSYLRRRWPQAAQLLEQVKRETS
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PGVIGRLLADFEPQ
CHHHHHHHHCCCCC
>Mature Secondary Structure
MRAMKLILAPMEGLADFWVRQALTDVGDYDWCVSEFVRVSGLLLPPKVFYRWCPELKLGA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
RTRAGTPVHLQLLGSDPACMADNAERAVELGAPAIDLNFGCPAKTVNRHRGGAVLLDEPD
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEECCCCCCHHHHHCCCCCEEEECCCH
VVHAVTAAVRQAVPSQVPVSVKMRLGNEDDARALDNARAVVDAGAAWLTVHGRTKRQGYK
HHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCC
PPAFWDRIGHIREVVSIPVIANGEIWTPDDAGRAQRESGCEDLMLGRGAVANPLLVSLLR
CCHHHHHHHHHHHHHHCCEEECCCEECCCCCCHHHHHCCCHHHHHCCCCCCHHHHHHHHH
FGGETNWDAILPILQQFWMDVADTGTDAQLAGRIKQWLSYLRRRWPQAAQLLEQVKRETS
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PGVIGRLLADFEPQ
CHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]