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The map label for this gene is cca

Identifier: 110834913

GI number: 110834913

Start: 2340824

End: 2342056

Strand: Direct

Name: cca

Synonym: ABO_2052

Alternate gene names: 110834913

Gene position: 2340824-2342056 (Clockwise)

Preceding gene: 110834911

Following gene: 110834914

Centisome position: 75.02

GC content: 56.37

Gene sequence:

>1233_bases
ATGAATGTCTATCTAGTGGGCGGCGCAGTGCGAGATTCGCTGCTCGGCCTCCCCGTGACGGAAAAAGATTGGGTCGTGGT
CGGTGCCACCCCGAAGGAAATGGAAGCCAACGGGTTCCGCCCCGTGGGCAAAGATTTCCCCGTATTCCTGCATCCAAAAA
CACAGGAAGAATACGCCCTGGCCAGAACCGAACGGAAATCCGGCCACGGTTATGGCGGCTTTACCTTCCATGCCGCCAGC
ACAGTCTCTCTAGAAGAGGATCTGATTCGCCGCGACCTGACCATCAATGCCATGGCGCAAGCCCGTGGTGGTGAAATCAT
CGACCCGTTCAATGGCCGCGTGGATCTCAAAGCTCGCCTATTGCGCCATGTTTCCCCCGCTTTTGCCGAAGACCCCTTAC
GAGTATTGCGGGTTGCCCGTTTTGCCGCTCGCTACCACTGGCTAGGGTTTCAGGTGGCCGATGACACACTTATGTTGATG
AAACAACTGAGCGACTCCGGTGAGCTGGGTTATCTTGTCGCTGAGCGGGTTTGGAAAGAAACCAGCCGAGCGCTCATGGA
GCGCGATCCTCAGGTATTCTTTCAGGTACTTCACCGCTGCGGCGCACTGCATGCCTTATTTCCAGAACTTGCCGCGTTGG
ATGGCGTACCTCAACCGGAACAACACCATCCGGAAGTGGACACCCTGCTTCATCAATTCCTATGCCTTAAGCAAGCCGCT
CGGCTCGGCTTAAGCCTTAACGCTCGCTATGCATTGCTCTGCCATGATCTAGGTAAGGGTAAAACCCCAAAGGAAGAGTG
GCCCCGACACATTGCCCACGAAATCCGCAGCGCTCGTTTAAGCAAAAAGGTATCAAAGCGATTGAAAGTTCCCAAGGAAG
CCGCAACCTTGGCCACCTTAGTCGCTGAGTTCCATACTCACAGCCACCGTGCATTGGAACTGAAACCGGCCACGGTGTGG
AAACTGTTCAAGTCGCTGGATATCCTTCGTCGCCCGGAGCGACTAACGGATTTCTTGGGAGCCTGTGAAGCCGACGCGCG
GGGCCGCACCAGTTTCGAAAACCGCGAGTATCCTCAAGCCTCCTATCTACAGGGCGCGGCAGATGCCGCACGCCAGGTAG
ATATACAGGCGCTGCAGGCCCAAGGCCACGAAGGTCCGGCACTGGGGGAAGCGATTGAGCAAGCCCGCATTGATGCCATC
GCCACCTTTAAACAACAGGAGCTGAACCCCTGA

Upstream 100 bases:

>100_bases
CTCGCCATTGGCGAGTACAGTGGTCTACGACTAAAGAGTTCAGTTTCAGTACACTAGAAAGCATGCGACCATTCTGCTCA
AGATAACGAGAGCGCACAAG

Downstream 100 bases:

>100_bases
TGAACGCCCCCGTCGCCCTGGTTACCGGCAGCGCCCGCCGTATTGGCGCCCAGATTGTTCGCACCCTGCATCAGCACGGG
ATGCGGGTGATCATTCATTA

Product: tRNA cytidylyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNVYLVGGAVRDSLLGLPVTEKDWVVVGATPKEMEANGFRPVGKDFPVFLHPKTQEEYALARTERKSGHGYGGFTFHAAS
TVSLEEDLIRRDLTINAMAQARGGEIIDPFNGRVDLKARLLRHVSPAFAEDPLRVLRVARFAARYHWLGFQVADDTLMLM
KQLSDSGELGYLVAERVWKETSRALMERDPQVFFQVLHRCGALHALFPELAALDGVPQPEQHHPEVDTLLHQFLCLKQAA
RLGLSLNARYALLCHDLGKGKTPKEEWPRHIAHEIRSARLSKKVSKRLKVPKEAATLATLVAEFHTHSHRALELKPATVW
KLFKSLDILRRPERLTDFLGACEADARGRTSFENREYPQASYLQGAADAARQVDIQALQAQGHEGPALGEAIEQARIDAI
ATFKQQELNP

Sequences:

>Translated_410_residues
MNVYLVGGAVRDSLLGLPVTEKDWVVVGATPKEMEANGFRPVGKDFPVFLHPKTQEEYALARTERKSGHGYGGFTFHAAS
TVSLEEDLIRRDLTINAMAQARGGEIIDPFNGRVDLKARLLRHVSPAFAEDPLRVLRVARFAARYHWLGFQVADDTLMLM
KQLSDSGELGYLVAERVWKETSRALMERDPQVFFQVLHRCGALHALFPELAALDGVPQPEQHHPEVDTLLHQFLCLKQAA
RLGLSLNARYALLCHDLGKGKTPKEEWPRHIAHEIRSARLSKKVSKRLKVPKEAATLATLVAEFHTHSHRALELKPATVW
KLFKSLDILRRPERLTDFLGACEADARGRTSFENREYPQASYLQGAADAARQVDIQALQAQGHEGPALGEAIEQARIDAI
ATFKQQELNP
>Mature_410_residues
MNVYLVGGAVRDSLLGLPVTEKDWVVVGATPKEMEANGFRPVGKDFPVFLHPKTQEEYALARTERKSGHGYGGFTFHAAS
TVSLEEDLIRRDLTINAMAQARGGEIIDPFNGRVDLKARLLRHVSPAFAEDPLRVLRVARFAARYHWLGFQVADDTLMLM
KQLSDSGELGYLVAERVWKETSRALMERDPQVFFQVLHRCGALHALFPELAALDGVPQPEQHHPEVDTLLHQFLCLKQAA
RLGLSLNARYALLCHDLGKGKTPKEEWPRHIAHEIRSARLSKKVSKRLKVPKEAATLATLVAEFHTHSHRALELKPATVW
KLFKSLDILRRPERLTDFLGACEADARGRTSFENREYPQASYLQGAADAARQVDIQALQAQGHEGPALGEAIEQARIDAI
ATFKQQELNP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=57.6354679802956, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_ALCBS (Q0VMU8)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693772.1
- ProteinModelPortal:   Q0VMU8
- SMR:   Q0VMU8
- STRING:   Q0VMU8
- GeneID:   4212963
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2052
- NMPDR:   fig|393595.12.peg.2054
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   Q0VMU8
- BioCyc:   ABOR393595:ABO_2052-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45874; Mature: 45874

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYLVGGAVRDSLLGLPVTEKDWVVVGATPKEMEANGFRPVGKDFPVFLHPKTQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHH
ARTERKSGHGYGGFTFHAASTVSLEEDLIRRDLTINAMAQARGGEIIDPFNGRVDLKARL
HHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYHWLGFQVADDTLMLMKQLSDSGELGYLVAERVWKE
HHHCCHHHHHCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHH
TSRALMERDPQVFFQVLHRCGALHALFPELAALDGVPQPEQHHPEVDTLLHQFLCLKQAA
HHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLGLSLNARYALLCHDLGKGKTPKEEWPRHIAHEIRSARLSKKVSKRLKVPKEAATLATL
HCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VAEFHTHSHRALELKPATVWKLFKSLDILRRPERLTDFLGACEADARGRTSFENREYPQA
HHHHHCCCCCEEEECHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SYLQGAADAARQVDIQALQAQGHEGPALGEAIEQARIDAIATFKQQELNP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVYLVGGAVRDSLLGLPVTEKDWVVVGATPKEMEANGFRPVGKDFPVFLHPKTQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHH
ARTERKSGHGYGGFTFHAASTVSLEEDLIRRDLTINAMAQARGGEIIDPFNGRVDLKARL
HHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYHWLGFQVADDTLMLMKQLSDSGELGYLVAERVWKE
HHHCCHHHHHCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHH
TSRALMERDPQVFFQVLHRCGALHALFPELAALDGVPQPEQHHPEVDTLLHQFLCLKQAA
HHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLGLSLNARYALLCHDLGKGKTPKEEWPRHIAHEIRSARLSKKVSKRLKVPKEAATLATL
HCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VAEFHTHSHRALELKPATVWKLFKSLDILRRPERLTDFLGACEADARGRTSFENREYPQA
HHHHHCCCCCEEEECHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SYLQGAADAARQVDIQALQAQGHEGPALGEAIEQARIDAIATFKQQELNP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA