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The map label for this gene is ksgA

Identifier: 110834909

GI number: 110834909

Start: 2337360

End: 2338202

Strand: Direct

Name: ksgA

Synonym: ABO_2048

Alternate gene names: 110834909

Gene position: 2337360-2338202 (Clockwise)

Preceding gene: 110834908

Following gene: 110834910

Centisome position: 74.91

GC content: 58.96

Gene sequence:

>843_bases
ATGAAAGAACATCGCACCCGTAAACGCTTTGGCCAGCATTTTCTGCACGACCGCAACTTGGTCGACCGCATGATCCGCAC
CCTGGGCCTGCAAACGGGAGACACCTTGGTGGAAATCGGCCCAGGCAGAGGCGCCCTCACCTATCCACTACTGGAAGAGC
TCCCCCATTTGCATGTGGTGGAACTGGACCGGGATCTAATCGCCCTACTGCGCCAAGAAAATACTCCCGAGCGACTGACC
ATCCACGAAAGTGATGCCCTGCGCTTTGATTTCCGCACCCTAAAACCAGCGGACAAGCCCCTGCGGGTGATCGGTAACCT
GCCGTACAACATCTCTACCCCCTTGATCTTTCATCTACTTGCCCAGGCCGACGCCATCAGCGACATGACTTTCATGCTGC
AAAAAGAAGTGGTGGAACGGCTCACTGCCAGCCCCGGCACCCGTGACTGGGGGCGGCTCTCGATCATGGTGCAGTATCAT
TGCCAGGCGGATTATCTTTTCTTTGTGCCCCCGGAGGCATTCAGCCCCCCTCCACGAGTAGACTCCGCCGTGGTCCGGCT
AATTCCCCACGACAGCCCGCCCCACCCAGCGGACGACGAAGACCACCTGCGTAAACTGGTGGCCCAGGCCTTTACCCAGC
GCCGCAAGGCAATCCGAAATGGGTTAAAATCTTGGGTTAGCGCCGAGCAATTTCAAGCCGTCGGCATTGATGCCGGCCTA
CGCCCGGATCAACTGTCCGTGGCCGACTACGTGGCGCTGGCCAACATCAGCCGCCCGCCGGCCGATGTGGAAGACGCCAA
CGCACCACACACTGAACAAGGCAAGGGAGACAACAGCCAATGA

Upstream 100 bases:

>100_bases
GCCTAGCGTTAACCTTGGCAACACCGCAGCATAATGCCTGACCACACCAGCGCCCTTGCGTTGATTAAGTCACACCACCC
GGCCTGACCTGAGATCCGCT

Downstream 100 bases:

>100_bases
GCGTCACCGAACCAGGTAGCGACAACACCGCCAACGGCGACTCCAATCACAACATACAGATATCGGTGGAAACCGAATTT
CTACCCGATCAGAGTGATGA

Product: ribosomal RNA adenine dimethylase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKEHRTRKRFGQHFLHDRNLVDRMIRTLGLQTGDTLVEIGPGRGALTYPLLEELPHLHVVELDRDLIALLRQENTPERLT
IHESDALRFDFRTLKPADKPLRVIGNLPYNISTPLIFHLLAQADAISDMTFMLQKEVVERLTASPGTRDWGRLSIMVQYH
CQADYLFFVPPEAFSPPPRVDSAVVRLIPHDSPPHPADDEDHLRKLVAQAFTQRRKAIRNGLKSWVSAEQFQAVGIDAGL
RPDQLSVADYVALANISRPPADVEDANAPHTEQGKGDNSQ

Sequences:

>Translated_280_residues
MKEHRTRKRFGQHFLHDRNLVDRMIRTLGLQTGDTLVEIGPGRGALTYPLLEELPHLHVVELDRDLIALLRQENTPERLT
IHESDALRFDFRTLKPADKPLRVIGNLPYNISTPLIFHLLAQADAISDMTFMLQKEVVERLTASPGTRDWGRLSIMVQYH
CQADYLFFVPPEAFSPPPRVDSAVVRLIPHDSPPHPADDEDHLRKLVAQAFTQRRKAIRNGLKSWVSAEQFQAVGIDAGL
RPDQLSVADYVALANISRPPADVEDANAPHTEQGKGDNSQ
>Mature_280_residues
MKEHRTRKRFGQHFLHDRNLVDRMIRTLGLQTGDTLVEIGPGRGALTYPLLEELPHLHVVELDRDLIALLRQENTPERLT
IHESDALRFDFRTLKPADKPLRVIGNLPYNISTPLIFHLLAQADAISDMTFMLQKEVVERLTASPGTRDWGRLSIMVQYH
CQADYLFFVPPEAFSPPPRVDSAVVRLIPHDSPPHPADDEDHLRKLVAQAFTQRRKAIRNGLKSWVSAEQFQAVGIDAGL
RPDQLSVADYVALANISRPPADVEDANAPHTEQGKGDNSQ

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=283, Percent_Identity=33.9222614840989, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI7657198, Length=250, Percent_Identity=31.2, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786236, Length=254, Percent_Identity=51.5748031496063, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25146882, Length=188, Percent_Identity=34.5744680851064, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25141369, Length=249, Percent_Identity=32.1285140562249, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=219, Percent_Identity=31.5068493150685, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357273, Length=296, Percent_Identity=32.0945945945946, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21358017, Length=193, Percent_Identity=37.8238341968912, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ALCBS (Q0VMV2)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693768.1
- ProteinModelPortal:   Q0VMV2
- SMR:   Q0VMV2
- STRING:   Q0VMV2
- GeneID:   4211533
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2048
- NMPDR:   fig|393595.12.peg.2050
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q0VMV2
- BioCyc:   ABOR393595:ABO_2048-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31601; Mature: 31601

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 13-13 BINDING 15-15 BINDING 40-40 BINDING 61-61 BINDING 85-85 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHRTRKRFGQHFLHDRNLVDRMIRTLGLQTGDTLVEIGPGRGALTYPLLEELPHLHVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECHHHHHCCCCEEE
ELDRDLIALLRQENTPERLTIHESDALRFDFRTLKPADKPLRVIGNLPYNISTPLIFHLL
EHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
AQADAISDMTFMLQKEVVERLTASPGTRDWGRLSIMVQYHCQADYLFFVPPEAFSPPPRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCC
DSAVVRLIPHDSPPHPADDEDHLRKLVAQAFTQRRKAIRNGLKSWVSAEQFQAVGIDAGL
CCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPDQLSVADYVALANISRPPADVEDANAPHTEQGKGDNSQ
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEHRTRKRFGQHFLHDRNLVDRMIRTLGLQTGDTLVEIGPGRGALTYPLLEELPHLHVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECHHHHHCCCCEEE
ELDRDLIALLRQENTPERLTIHESDALRFDFRTLKPADKPLRVIGNLPYNISTPLIFHLL
EHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
AQADAISDMTFMLQKEVVERLTASPGTRDWGRLSIMVQYHCQADYLFFVPPEAFSPPPRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCC
DSAVVRLIPHDSPPHPADDEDHLRKLVAQAFTQRRKAIRNGLKSWVSAEQFQAVGIDAGL
CCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPDQLSVADYVALANISRPPADVEDANAPHTEQGKGDNSQ
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA