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The map label for this gene is nemA [C]

Identifier: 110834900

GI number: 110834900

Start: 2327231

End: 2328340

Strand: Reverse

Name: nemA [C]

Synonym: ABO_2039

Alternate gene names: 110834900

Gene position: 2328340-2327231 (Counterclockwise)

Preceding gene: 110834902

Following gene: 110834898

Centisome position: 74.62

GC content: 54.5

Gene sequence:

>1110_bases
ATGAGTAGTTCCCTGGATAGCCTGTTGCGTCCTTTTGAACTTAAGTCTTTGAAAATGCGTAATCGAGTGGCCATGGCGCC
CATGACGCGCACCTTTTCTCCGGGTTATGTGCCTAATGATCAGGTGGTGGGCTATTACCAGCGCCGAGCCGAGGGCGAGG
TGGGCCTGATCATTACCGAGGGTACTTTTGTGGGCCACAAGGCCGCCAATGGTTATGAGGGTGTGCCGGCTATTTATGGC
GATGAGGCCATGGCGGGTTGGAAGAAAGTGGTGGACGCGGTGCACGCTGCCGGTGGGCAGATCATTCCACAGTTGTGGCA
TGTGGGCTCAGTGCGTAAGCCGGGTATTGGCCCGGACAAGGATGTGCCTGCTTATTCGCCTTCCGGTTTGTTTAAGCCGG
GTAAAAAAAATGGCGAAGCTATGAGCAAAGCGGATATCCAGGATGTGATTCGTGCTTTTGCTGAATCTGCCAAGGCGGCT
AAAGAGGTGGGCTTTGATGGGGTCGAGATTCATGGTGCCCATGGTTATCTACTCGATCAGTTTTTCTGGGAAGGCACCAA
CCAGCGTGATGATGAGTACGGTGGGTCCATCGAGAACCGTGCCCGTTTTGGTATCGAGATTGTTAAGGCGGTACGCGAGG
CGGTAGGTGAGGACTTCGCCATCGTGCTGCGTTTTTCCCAGTGGAAGCAGCAAGATTACGAGGCAAAGCTGGCTCATAAT
CCAGAAGAGCTGGAGCGCTTCCTGATGCCGTTTGTGGAGGCCGGTGTCGATGTGTTTCACGCGTCTACGCGCCGTTTTTG
GTTGCCAGAATTTGAGGGGTCCGATCTGAACTTGGCAGGTTGGACTAAAAAGATTACGGGTAAGCCGGTGATTTCTGTGG
GCAGCGTGGGTTTGGATGGCGACTTCCTCAATAGCCCAGAAGGCATGGGTGGAACGGCTAACCCGACGGGGCTTGATGAG
CTGGAGCGGCGCATGGATAACGACGAGTTCGACATAATTGCCGTGGGCCGTGCGTTGCTGCAGGACCCGAACTGGTTGGT
GAAAATACGTGAGGGTAAGGAAGACGAAATTGCGCCGTTCACTAAGGCTGCGCTGGGCAGCTTGAGTTAA

Upstream 100 bases:

>100_bases
CATTGCTAGCCCCTTTTTTTGTTACCTCCAATGCTCACGCCGAGCCAGGTACGGGCTCGGCTTAGAGAGTGAATAAACGA
AGATTGATTAGGAGAGCCTC

Downstream 100 bases:

>100_bases
GAGTGAAAGGTTAAACGTTGGAATGCGGGCCGGGGTTTACCGGGGCCGATAACATAGAGGCCGCATCCAGATCTGGACGC
GGCCTCTTTCGTTTCAGTAA

Product: FMN-binding oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSSSLDSLLRPFELKSLKMRNRVAMAPMTRTFSPGYVPNDQVVGYYQRRAEGEVGLIITEGTFVGHKAANGYEGVPAIYG
DEAMAGWKKVVDAVHAAGGQIIPQLWHVGSVRKPGIGPDKDVPAYSPSGLFKPGKKNGEAMSKADIQDVIRAFAESAKAA
KEVGFDGVEIHGAHGYLLDQFFWEGTNQRDDEYGGSIENRARFGIEIVKAVREAVGEDFAIVLRFSQWKQQDYEAKLAHN
PEELERFLMPFVEAGVDVFHASTRRFWLPEFEGSDLNLAGWTKKITGKPVISVGSVGLDGDFLNSPEGMGGTANPTGLDE
LERRMDNDEFDIIAVGRALLQDPNWLVKIREGKEDEIAPFTKAALGSLS

Sequences:

>Translated_369_residues
MSSSLDSLLRPFELKSLKMRNRVAMAPMTRTFSPGYVPNDQVVGYYQRRAEGEVGLIITEGTFVGHKAANGYEGVPAIYG
DEAMAGWKKVVDAVHAAGGQIIPQLWHVGSVRKPGIGPDKDVPAYSPSGLFKPGKKNGEAMSKADIQDVIRAFAESAKAA
KEVGFDGVEIHGAHGYLLDQFFWEGTNQRDDEYGGSIENRARFGIEIVKAVREAVGEDFAIVLRFSQWKQQDYEAKLAHN
PEELERFLMPFVEAGVDVFHASTRRFWLPEFEGSDLNLAGWTKKITGKPVISVGSVGLDGDFLNSPEGMGGTANPTGLDE
LERRMDNDEFDIIAVGRALLQDPNWLVKIREGKEDEIAPFTKAALGSLS
>Mature_368_residues
SSSLDSLLRPFELKSLKMRNRVAMAPMTRTFSPGYVPNDQVVGYYQRRAEGEVGLIITEGTFVGHKAANGYEGVPAIYGD
EAMAGWKKVVDAVHAAGGQIIPQLWHVGSVRKPGIGPDKDVPAYSPSGLFKPGKKNGEAMSKADIQDVIRAFAESAKAAK
EVGFDGVEIHGAHGYLLDQFFWEGTNQRDDEYGGSIENRARFGIEIVKAVREAVGEDFAIVLRFSQWKQQDYEAKLAHNP
EELERFLMPFVEAGVDVFHASTRRFWLPEFEGSDLNLAGWTKKITGKPVISVGSVGLDGDFLNSPEGMGGTANPTGLDEL
ERRMDNDEFDIIAVGRALLQDPNWLVKIREGKEDEIAPFTKAALGSLS

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=232, Percent_Identity=38.3620689655172, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1789463, Length=362, Percent_Identity=27.9005524861878, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17540738, Length=241, Percent_Identity=31.1203319502075, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=187, Percent_Identity=33.1550802139037, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=211, Percent_Identity=31.7535545023697, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=199, Percent_Identity=32.6633165829146, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=221, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=217, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=212, Percent_Identity=33.4905660377358, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=235, Percent_Identity=36.5957446808511, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321973, Length=235, Percent_Identity=36.1702127659575, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40479; Mature: 40348

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLDSLLRPFELKSLKMRNRVAMAPMTRTFSPGYVPNDQVVGYYQRRAEGEVGLIITE
CCCCHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
GTFVGHKAANGYEGVPAIYGDEAMAGWKKVVDAVHAAGGQIIPQLWHVGSVRKPGIGPDK
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
DVPAYSPSGLFKPGKKNGEAMSKADIQDVIRAFAESAKAAKEVGFDGVEIHGAHGYLLDQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
FFWEGTNQRDDEYGGSIENRARFGIEIVKAVREAVGEDFAIVLRFSQWKQQDYEAKLAHN
HHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCHHHHCCC
PEELERFLMPFVEAGVDVFHASTRRFWLPEFEGSDLNLAGWTKKITGKPVISVGSVGLDG
HHHHHHHHHHHHHHCHHHEECCCCEEECCCCCCCCCEECCCCCCCCCCCEEEECCCCCCC
DFLNSPEGMGGTANPTGLDELERRMDNDEFDIIAVGRALLQDPNWLVKIREGKEDEIAPF
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHCCCCEEEEEECCCCCCCCHH
TKAALGSLS
HHHHHCCCC
>Mature Secondary Structure 
SSSLDSLLRPFELKSLKMRNRVAMAPMTRTFSPGYVPNDQVVGYYQRRAEGEVGLIITE
CCCHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
GTFVGHKAANGYEGVPAIYGDEAMAGWKKVVDAVHAAGGQIIPQLWHVGSVRKPGIGPDK
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
DVPAYSPSGLFKPGKKNGEAMSKADIQDVIRAFAESAKAAKEVGFDGVEIHGAHGYLLDQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
FFWEGTNQRDDEYGGSIENRARFGIEIVKAVREAVGEDFAIVLRFSQWKQQDYEAKLAHN
HHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCHHHHCCC
PEELERFLMPFVEAGVDVFHASTRRFWLPEFEGSDLNLAGWTKKITGKPVISVGSVGLDG
HHHHHHHHHHHHHHCHHHEECCCCEEECCCCCCCCCEECCCCCCCCCCCEEEECCCCCCC
DFLNSPEGMGGTANPTGLDELERRMDNDEFDIIAVGRALLQDPNWLVKIREGKEDEIAPF
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHCCCCEEEEEECCCCCCCCHH
TKAALGSLS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]