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The map label for this gene is dusB [H]

Identifier: 110834874

GI number: 110834874

Start: 2299530

End: 2300570

Strand: Direct

Name: dusB [H]

Synonym: ABO_2013

Alternate gene names: 110834874

Gene position: 2299530-2300570 (Clockwise)

Preceding gene: 110834873

Following gene: 110834875

Centisome position: 73.7

GC content: 59.46

Gene sequence:

>1041_bases
ATGCAGATCGGACCCTATAACCTGCCGAACTCCTTGATTCTAGCCCCCATGGCCGGCGTCACGGACCGCCCATTCCGGCG
CTTGTGCAGAGACCTGGGGGCCGGCCTGCTGGTGTCGGAAATGGTGACCTCGGACACCCGTCTGTGGAACTCTCGAAAGT
CTCGCCAACGGTTAGACCATAGCGGCGAACCAGGCCCCATCTCGGTACAGATCGCCGGCGGAGAGCCGGCAATGCTGGCA
GAGGCGGCCCGCGCCAACGTGGAGCATGGCGCCCAGATTATTGACATCAATATGGGCTGCCCGGCGAAAAAAGTGTGCAA
ACGCGCCGCGGGGTCTGCCCTGCTAGCCGACCCGGACCTAGTCGCGCGCATCCTCGATGCAGTGACTCAGGCTGTAAATG
TCCCCGTTACCCTGAAAATCCGTACCGGCCCTGATCGCGATCAGCGCAATGCCATCGACATTGCCCGCATCGCCGAAACT
GCGGGCATTCAAGCCCTTGCCATTCACGGGCGCACCCGCGCCGACAAGTTCAACGGTGACGCCGAATACGCCACTATCAA
ACAGGTGGTGGATGCGGTGAACATTCCGATCATGGCCAATGGAGACGTGGATAGTCCGGAAAAAGCCAAACGGGTACTGG
AATCCACCGGCGCCGATGGTATCATGATCGGCCGCGCTGCTCAGGGTAACCCGTGGATCTTCCGGGACACCCTGTATTAC
CTCGAGCACGGCTCGCTGCCCGCAGCGCCGTTAGTAACGGAAGTCCATGATTGCGTGCAACAGCACCTGTACGAACTGCA
TGATTTTTATGGTGAGTTCATGGGGGTACGTATCGCACGCAAGCACCTGGGCTGGTACACACAGAACCTGCCCAATGGGG
AAGACTTCCGGCGCGGGTTTAACACACTGGAAAATGCCACCGATCAACGCGTGGCAATCGAGCAGTTTTTCCAACAGATA
AGCGCCACTACCGACCGAGTTCGCTTCGGTCGTTATGGCGAGGTGTCTGCCCGGAAGAAAAACGGAGCCCTGGCCGCATG
A

Upstream 100 bases:

>100_bases
CAAAATCGACAGCCCGTCGCCTGATGGCAGACGGGCCTTTCATCCCTAGCCTCCGATCTCTATCATTGTTCCCCCTCTTA
GACACCCCAAGCCCGGCGCC

Downstream 100 bases:

>100_bases
CCCTTTCTTCAGTTAACACAGCCGCTGCACGGACACTTACTGAAATGAACACCGCTGAAGCACGAAAGCAGCCGCATCTT
ACTATTGCGCGCAGCGAAAC

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MQIGPYNLPNSLILAPMAGVTDRPFRRLCRDLGAGLLVSEMVTSDTRLWNSRKSRQRLDHSGEPGPISVQIAGGEPAMLA
EAARANVEHGAQIIDINMGCPAKKVCKRAAGSALLADPDLVARILDAVTQAVNVPVTLKIRTGPDRDQRNAIDIARIAET
AGIQALAIHGRTRADKFNGDAEYATIKQVVDAVNIPIMANGDVDSPEKAKRVLESTGADGIMIGRAAQGNPWIFRDTLYY
LEHGSLPAAPLVTEVHDCVQQHLYELHDFYGEFMGVRIARKHLGWYTQNLPNGEDFRRGFNTLENATDQRVAIEQFFQQI
SATTDRVRFGRYGEVSARKKNGALAA

Sequences:

>Translated_346_residues
MQIGPYNLPNSLILAPMAGVTDRPFRRLCRDLGAGLLVSEMVTSDTRLWNSRKSRQRLDHSGEPGPISVQIAGGEPAMLA
EAARANVEHGAQIIDINMGCPAKKVCKRAAGSALLADPDLVARILDAVTQAVNVPVTLKIRTGPDRDQRNAIDIARIAET
AGIQALAIHGRTRADKFNGDAEYATIKQVVDAVNIPIMANGDVDSPEKAKRVLESTGADGIMIGRAAQGNPWIFRDTLYY
LEHGSLPAAPLVTEVHDCVQQHLYELHDFYGEFMGVRIARKHLGWYTQNLPNGEDFRRGFNTLENATDQRVAIEQFFQQI
SATTDRVRFGRYGEVSARKKNGALAA
>Mature_346_residues
MQIGPYNLPNSLILAPMAGVTDRPFRRLCRDLGAGLLVSEMVTSDTRLWNSRKSRQRLDHSGEPGPISVQIAGGEPAMLA
EAARANVEHGAQIIDINMGCPAKKVCKRAAGSALLADPDLVARILDAVTQAVNVPVTLKIRTGPDRDQRNAIDIARIAET
AGIQALAIHGRTRADKFNGDAEYATIKQVVDAVNIPIMANGDVDSPEKAKRVLESTGADGIMIGRAAQGNPWIFRDTLYY
LEHGSLPAAPLVTEVHDCVQQHLYELHDFYGEFMGVRIARKHLGWYTQNLPNGEDFRRGFNTLENATDQRVAIEQFFQQI
SATTDRVRFGRYGEVSARKKNGALAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=230, Percent_Identity=31.7391304347826, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI40807366, Length=256, Percent_Identity=32.8125, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=32.2033898305085, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.2222222222222, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=31.1475409836066, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=57.7639751552795, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=31.0924369747899, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=28.7096774193548, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=310, Percent_Identity=29.3548387096774, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17507177, Length=244, Percent_Identity=31.9672131147541, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=230, Percent_Identity=30.8695652173913, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=29.5546558704453, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=33.1896551724138, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323437, Length=327, Percent_Identity=25.6880733944954, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=322, Percent_Identity=26.3975155279503, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24580595, Length=274, Percent_Identity=29.9270072992701, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19920448, Length=274, Percent_Identity=29.9270072992701, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921524, Length=298, Percent_Identity=30.5369127516779, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=31.2236286919831, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45549423, Length=291, Percent_Identity=31.9587628865979, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37850; Mature: 37850

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYNLPNSLILAPMAGVTDRPFRRLCRDLGAGLLVSEMVTSDTRLWNSRKSRQRLDH
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SGEPGPISVQIAGGEPAMLAEAARANVEHGAQIIDINMGCPAKKVCKRAAGSALLADPDL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEECCHHH
VARILDAVTQAVNVPVTLKIRTGPDRDQRNAIDIARIAETAGIQALAIHGRTRADKFNGD
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
AEYATIKQVVDAVNIPIMANGDVDSPEKAKRVLESTGADGIMIGRAAQGNPWIFRDTLYY
CHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEHHHHHH
LEHGSLPAAPLVTEVHDCVQQHLYELHDFYGEFMGVRIARKHLGWYTQNLPNGEDFRRGF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NTLENATDQRVAIEQFFQQISATTDRVRFGRYGEVSARKKNGALAA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIGPYNLPNSLILAPMAGVTDRPFRRLCRDLGAGLLVSEMVTSDTRLWNSRKSRQRLDH
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SGEPGPISVQIAGGEPAMLAEAARANVEHGAQIIDINMGCPAKKVCKRAAGSALLADPDL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEECCHHH
VARILDAVTQAVNVPVTLKIRTGPDRDQRNAIDIARIAETAGIQALAIHGRTRADKFNGD
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
AEYATIKQVVDAVNIPIMANGDVDSPEKAKRVLESTGADGIMIGRAAQGNPWIFRDTLYY
CHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEHHHHHH
LEHGSLPAAPLVTEVHDCVQQHLYELHDFYGEFMGVRIARKHLGWYTQNLPNGEDFRRGF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NTLENATDQRVAIEQFFQQISATTDRVRFGRYGEVSARKKNGALAA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]