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The map label for this gene is ppa [H]

Identifier: 110834855

GI number: 110834855

Start: 2282069

End: 2282599

Strand: Direct

Name: ppa [H]

Synonym: ABO_1994

Alternate gene names: 110834855

Gene position: 2282069-2282599 (Clockwise)

Preceding gene: 110834854

Following gene: 110834857

Centisome position: 73.14

GC content: 55.37

Gene sequence:

>531_bases
ATGGATTTTGCTAAGGTTCCCGCCGGGAAAGACCTCCCCGAAGACATCTACGTGGCCATCGAGATTCCGGCCAATGCTGA
CCCGATCAAGTACGAGATCGACAAAGACAGCAACGCCGTATTCGTAGACCGCTTCATGGCGACCCCCATGTTCTATCCGG
CCAACTACGGCTACATCCCCAATACCCTGTCCGAAGATGGCGACCCGCTGGACGTGCTGGTGGTAACGCCGTACCCAGTG
GTCCCCGGTTCCGTGATTCGCTGCCGCCCGGTAGGTATCCTGAACATGACCGACGAAGCCGGTAAAGATGCCAAGCTGGT
TGCAGTGCCGCACACCAAATTGACCAAAATCTACGACGACGTAAAAGACATCGACGACCTTCCCGAGCTGCTGTTGGCCC
AGATCAAGCACTTCTTCGAGCACTACAAAGAGCTGGAAGCAGGCAAGTGGGTGAAGGTTGATGGCTGGGATGGCGCCGAA
GCCGCCAAGGCCGAGATTATCAAATCTGTAGAAGCTTACGAAAAAGCGTAA

Upstream 100 bases:

>100_bases
TTCTATTCCATAGGCAAGTTCGACTGTCCCCGATTAGCCGTCGTCCGCTGCCATTGCTATAATCCGCGCCCACCACCCAC
CCTGTTGAGAAGGACAGACC

Downstream 100 bases:

>100_bases
GGTGAGCTTCAAGCGACAACGCGAGATAACTTCGCGGTAAGCAGAAAAAGGCCGCACCCATTATCTGGGCGCGGCCTTTT
TTATGTCCCTTTCTTGCAGC

Product: inorganic diphosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDFAKVPAGKDLPEDIYVAIEIPANADPIKYEIDKDSNAVFVDRFMATPMFYPANYGYIPNTLSEDGDPLDVLVVTPYPV
VPGSVIRCRPVGILNMTDEAGKDAKLVAVPHTKLTKIYDDVKDIDDLPELLLAQIKHFFEHYKELEAGKWVKVDGWDGAE
AAKAEIIKSVEAYEKA

Sequences:

>Translated_176_residues
MDFAKVPAGKDLPEDIYVAIEIPANADPIKYEIDKDSNAVFVDRFMATPMFYPANYGYIPNTLSEDGDPLDVLVVTPYPV
VPGSVIRCRPVGILNMTDEAGKDAKLVAVPHTKLTKIYDDVKDIDDLPELLLAQIKHFFEHYKELEAGKWVKVDGWDGAE
AAKAEIIKSVEAYEKA
>Mature_176_residues
MDFAKVPAGKDLPEDIYVAIEIPANADPIKYEIDKDSNAVFVDRFMATPMFYPANYGYIPNTLSEDGDPLDVLVVTPYPV
VPGSVIRCRPVGILNMTDEAGKDAKLVAVPHTKLTKIYDDVKDIDDLPELLLAQIKHFFEHYKELEAGKWVKVDGWDGAE
AAKAEIIKSVEAYEKA

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=171, Percent_Identity=77.7777777777778, Blast_Score=278, Evalue=1e-76,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19557; Mature: 19557

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFAKVPAGKDLPEDIYVAIEIPANADPIKYEIDKDSNAVFVDRFMATPMFYPANYGYIP
CCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEHHHHCCEECCCCCCCCC
NTLSEDGDPLDVLVVTPYPVVPGSVIRCRPVGILNMTDEAGKDAKLVAVPHTKLTKIYDD
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECCHHHHHHHHH
VKDIDDLPELLLAQIKHFFEHYKELEAGKWVKVDGWDGAEAAKAEIIKSVEAYEKA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFAKVPAGKDLPEDIYVAIEIPANADPIKYEIDKDSNAVFVDRFMATPMFYPANYGYIP
CCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEHHHHCCEECCCCCCCCC
NTLSEDGDPLDVLVVTPYPVVPGSVIRCRPVGILNMTDEAGKDAKLVAVPHTKLTKIYDD
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECCHHHHHHHHH
VKDIDDLPELLLAQIKHFFEHYKELEAGKWVKVDGWDGAEAAKAEIIKSVEAYEKA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]