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The map label for this gene is nth [H]

Identifier: 110834478

GI number: 110834478

Start: 1850810

End: 1851448

Strand: Direct

Name: nth [H]

Synonym: ABO_1617

Alternate gene names: 110834478

Gene position: 1850810-1851448 (Clockwise)

Preceding gene: 110834476

Following gene: 110834481

Centisome position: 59.32

GC content: 55.09

Gene sequence:

>639_bases
ATGAATCGCGAAAAACGTACTGAAATTTTTTCTCGTCTGCGTGCCCAGAATCCACACCCGACCACCGAACTGGAATACCA
GTCGGATTTCGAGCTACTGGTTGCCGTGGTGCTCTCAGCCCAGGCCACGGATGTGGGCGTCAACAAAGCCACCGCGCGGC
TATATCCGGTGGCGAATACGCCAGAGGCGATTTTCGCTCTAGGTGTAGATGGGCTCAAAGAGTACATCAAGACCATCGGC
CTGTTTAATTCCAAGGCCGAAAACATCATTCAACTGTGTAAAATATTGATCGAACAGCATGAGAGCCAGGTACCCCGCAC
CCGTGAAGAGCTAGAAGCCCTTCCCGGTGTTGGCCGCAAAACCGCCAACGTGGTGCTTAATACTGCCTTCGGCTACCCCA
CCATGGCCGTGGACACCCACATCTTCCGCGTCTCCAACCGCACCCGCATTGCTCCGGGTAAAAATGTGCTGGAGGTAGAA
AAACGCCTAGTGCGGCTGGTGCCGGAGGAGTTTCTACGTGACGCCCATCACTGGCTAATTCTGCATGGCCGCTATGTGTG
CACCGCCCGTAAACCCAAGTGCGGAGAATGCTTGATCGCTGATCTATGCGAGTTTCCGCCTCGCCAGCGGCCTAGCTAA

Upstream 100 bases:

>100_bases
GATCCGTCTATCCGGACCTGACACACCACCGTTCACAGGGGTTACCCCTCACAGTAGCCACTTGATTCGCTATACTGCCT
CGCGAACACAGGAAGATGAT

Downstream 100 bases:

>100_bases
CGGAGCATGGTGAGCCCCGCCATTAGCCGCCGGCTGCCCTCTTATAGTTAAAGGCTCAGCCGTATCTGATCCTGCCCGCG
TTGCTTCGCTTTCGGCAGCA

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNREKRTEIFSRLRAQNPHPTTELEYQSDFELLVAVVLSAQATDVGVNKATARLYPVANTPEAIFALGVDGLKEYIKTIG
LFNSKAENIIQLCKILIEQHESQVPRTREELEALPGVGRKTANVVLNTAFGYPTMAVDTHIFRVSNRTRIAPGKNVLEVE
KRLVRLVPEEFLRDAHHWLILHGRYVCTARKPKCGECLIADLCEFPPRQRPS

Sequences:

>Translated_212_residues
MNREKRTEIFSRLRAQNPHPTTELEYQSDFELLVAVVLSAQATDVGVNKATARLYPVANTPEAIFALGVDGLKEYIKTIG
LFNSKAENIIQLCKILIEQHESQVPRTREELEALPGVGRKTANVVLNTAFGYPTMAVDTHIFRVSNRTRIAPGKNVLEVE
KRLVRLVPEEFLRDAHHWLILHGRYVCTARKPKCGECLIADLCEFPPRQRPS
>Mature_212_residues
MNREKRTEIFSRLRAQNPHPTTELEYQSDFELLVAVVLSAQATDVGVNKATARLYPVANTPEAIFALGVDGLKEYIKTIG
LFNSKAENIIQLCKILIEQHESQVPRTREELEALPGVGRKTANVVLNTAFGYPTMAVDTHIFRVSNRTRIAPGKNVLEVE
KRLVRLVPEEFLRDAHHWLILHGRYVCTARKPKCGECLIADLCEFPPRQRPS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=72.1951219512195, Blast_Score=321, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.1428571428571, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.1336898395722, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=33.7078651685393, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23971; Mature: 23971

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREKRTEIFSRLRAQNPHPTTELEYQSDFELLVAVVLSAQATDVGVNKATARLYPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCC
PEAIFALGVDGLKEYIKTIGLFNSKAENIIQLCKILIEQHESQVPRTREELEALPGVGRK
CHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
TANVVLNTAFGYPTMAVDTHIFRVSNRTRIAPGKNVLEVEKRLVRLVPEEFLRDAHHWLI
HHHHEEECCCCCCCHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCTARKPKCGECLIADLCEFPPRQRPS
EECEEEEECCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNREKRTEIFSRLRAQNPHPTTELEYQSDFELLVAVVLSAQATDVGVNKATARLYPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCC
PEAIFALGVDGLKEYIKTIGLFNSKAENIIQLCKILIEQHESQVPRTREELEALPGVGRK
CHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
TANVVLNTAFGYPTMAVDTHIFRVSNRTRIAPGKNVLEVEKRLVRLVPEEFLRDAHHWLI
HHHHEEECCCCCCCHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCTARKPKCGECLIADLCEFPPRQRPS
EECEEEEECCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]