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The map label for this gene is rnfB

Identifier: 110834471

GI number: 110834471

Start: 1842462

End: 1843046

Strand: Direct

Name: rnfB

Synonym: ABO_1610

Alternate gene names: 110834471

Gene position: 1842462-1843046 (Clockwise)

Preceding gene: 110834470

Following gene: 110834472

Centisome position: 59.05

GC content: 60.68

Gene sequence:

>585_bases
ATGAGTGGTGTACTGATTGCTGTCGCCGCCCTGCTAGCCTTGGCCGCCGTGTTCGGTGCGGTGCTGGGTTTTGCCTCGGA
AAAGTTCAAAGTGGAAGGCGACCCGATCGTCGACCAAATCGACTCGTTGCTGCCCCAAACTCAATGCGGCCAGTGCGGCC
ATCCGGGCTGTCGCCCCTACGCAGAAGCCATTGCTGAAGGCGAAGAGCACAACCGCTGCCCGCCAGGGGGGCAAGACACG
GTTGTTGCGTTATCGGAACTATTGGGCCGCGAAGAACTGACGCTGGATGATGAAGGCGCGGAAGACGCTAACGTGGCCAA
GGTGGCCTACATCCGTGAAGACGAGTGTATTGGCTGCACCAAGTGCATCCAGGCCTGCCCGGTAGATGCCATTGTCGGCG
CCGCCAAGCTGATGCACACCGTCATTGTGGATGAGTGCACCGGCTGCGACCTCTGCGTGGAGCCCTGCCCGGTGGATTGC
ATTGATATGCTGGAAGTGAAGCCCACCCTGCAAACCTGGGGCTGGCAGCGCCCGGCCGGCATTGGTGAACGCCCGGACAG
CCGTATCGAGGTGGTGAATTTATGA

Upstream 100 bases:

>100_bases
TGGCCGATGTGCCCGAAGCTTTTCGCGGCCCCAGCATCGGCCTGATTACCGCTGGCTTGATGTCGCTGGCGTTCATGGGC
TTTTCCGGGTTGATCAAGCT

Downstream 100 bases:

>100_bases
CACACCCGATACCTAACACCACGCGCATGATGCGGGTGCTTTGCCAACCTGCTCCGCTCGCCCATGGCGGGAGGTTTTTG
CTATGAGTCAGGCCCAAACC

Product: electron transport complex protein RnfB

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSGVLIAVAALLALAAVFGAVLGFASEKFKVEGDPIVDQIDSLLPQTQCGQCGHPGCRPYAEAIAEGEEHNRCPPGGQDT
VVALSELLGREELTLDDEGAEDANVAKVAYIREDECIGCTKCIQACPVDAIVGAAKLMHTVIVDECTGCDLCVEPCPVDC
IDMLEVKPTLQTWGWQRPAGIGERPDSRIEVVNL

Sequences:

>Translated_194_residues
MSGVLIAVAALLALAAVFGAVLGFASEKFKVEGDPIVDQIDSLLPQTQCGQCGHPGCRPYAEAIAEGEEHNRCPPGGQDT
VVALSELLGREELTLDDEGAEDANVAKVAYIREDECIGCTKCIQACPVDAIVGAAKLMHTVIVDECTGCDLCVEPCPVDC
IDMLEVKPTLQTWGWQRPAGIGERPDSRIEVVNL
>Mature_193_residues
SGVLIAVAALLALAAVFGAVLGFASEKFKVEGDPIVDQIDSLLPQTQCGQCGHPGCRPYAEAIAEGEEHNRCPPGGQDTV
VALSELLGREELTLDDEGAEDANVAKVAYIREDECIGCTKCIQACPVDAIVGAAKLMHTVIVDECTGCDLCVEPCPVDCI
DMLEVKPTLQTWGWQRPAGIGERPDSRIEVVNL

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1787915, Length=176, Percent_Identity=57.3863636363636, Blast_Score=209, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFB_ALCBS (Q0VP40)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693330.1
- ProteinModelPortal:   Q0VP40
- SMR:   Q0VP40
- STRING:   Q0VP40
- GeneID:   4212122
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1610
- NMPDR:   fig|393595.12.peg.1613
- eggNOG:   COG2878
- HOGENOM:   HBG549899
- OMA:   KFKVEGD
- PhylomeDB:   Q0VP40
- BioCyc:   ABOR393595:ABO_1610-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00463
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- PIRSF:   PIRSF005784
- TIGRFAMs:   TIGR01944

Pfam domain/function: PF00037 Fer4; PF04060 FeS

EC number: NA

Molecular weight: Translated: 20564; Mature: 20432

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
6.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVLIAVAALLALAAVFGAVLGFASEKFKVEGDPIVDQIDSLLPQTQCGQCGHPGCRPY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
AEAIAEGEEHNRCPPGGQDTVVALSELLGREELTLDDEGAEDANVAKVAYIREDECIGCT
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
KCIQACPVDAIVGAAKLMHTVIVDECTGCDLCVEPCPVDCIDMLEVKPTLQTWGWQRPAG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCHHHHCCCCCCCC
IGERPDSRIEVVNL
CCCCCCCCEEEECC
>Mature Secondary Structure 
SGVLIAVAALLALAAVFGAVLGFASEKFKVEGDPIVDQIDSLLPQTQCGQCGHPGCRPY
CHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
AEAIAEGEEHNRCPPGGQDTVVALSELLGREELTLDDEGAEDANVAKVAYIREDECIGCT
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
KCIQACPVDAIVGAAKLMHTVIVDECTGCDLCVEPCPVDCIDMLEVKPTLQTWGWQRPAG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCHHHHCCCCCCCC
IGERPDSRIEVVNL
CCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA