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The map label for this gene is mmsB [C]

Identifier: 110834364

GI number: 110834364

Start: 1716109

End: 1716990

Strand: Direct

Name: mmsB [C]

Synonym: ABO_1503

Alternate gene names: 110834364

Gene position: 1716109-1716990 (Clockwise)

Preceding gene: 110834363

Following gene: 110834366

Centisome position: 55.0

GC content: 57.48

Gene sequence:

>882_bases
ATGAGCACACTAAACGCGGCTTTTATCGGCCTTGGCACCATGGGCTACCCCATGGCTGGCCACCTGGCCGAAGCAGGCAT
TGCCATGACCGTTTACAACCGCACGGCCCAACGAGCGCAACAGTGGACGCGCGATTACCCAGGCAGTCACGCGCCCTCCC
CCGCAGACGCCGCACGGCATGCGGATCTGGTCTTTATTTGCGTCGGAGACGATAACGATCTACGCCAGGTCACCCTTGGT
GATGACGGGGTTATGCAAACTCTCGCCAAGGGCGCTGTTCTTGTGGATCACACCACCGCCAGTGCAGAAGTGGCCCGCGA
ACTTGATGCCGCCAGTCGCAAACAAGGCAGTCATTTTCTCGATGCCCCGGTGTCTGGGGGCCAACAGGGTGCACAGAACG
GCCAATTATCGATCATGTGTGGCGGAGACGAAGCCACCTATCAGCGGGTACTGCCAACGCTGCAACATTACGGCAAAGCC
ATCAATCTGATGGGCCCGGCAGGCAGTGGCCAGCTAACCAAGATGGTCAACCAAATTTGCGTGGCCGGGCTGGTAGAAGG
GCTCGCTGAAGGACTCGCTTTCGCTCTTAATGCGGGCCTTGATGCCAACAAAGTCTTCGAAACCATCAGCCAGGGTGCAG
CCGGCTCCTGGCAAATGGTGAACCGCCACCAGACCATGATCGACGACCAGTATGAGCACGGTTTTGCGGTGGATTGGATG
CGAAAAGACTTGGATATCTGTCTGCGCGAAGCGGTTCGAAACGGCACACCCTTGCCCATCACTGAACAAATAAACGACTT
TTACCGTGAGATTCAGTCCATGGGCGGCGGCCGCTGGGACACCTCTTCGCTACTCCGTCGACTGCAAAAAATCCCCACTT
AA

Upstream 100 bases:

>100_bases
GCAGCCTCACCGAACCTCGCTTCGCCGCTTTCACCCTGCCCGGCACAGCAACAGCAACAGCAACAGCAACAGCAACAGTC
CACGATGGAGCCAACAAACA

Downstream 100 bases:

>100_bases
TAGCAAATATTGCCCTCTTACGGTTTTCGAACCGCTAACGTCTTCACCATGGGAGTCTAATTTTCCACACATTAAGAGCC
TGAGAACGAGGAAACGATAT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSTLNAAFIGLGTMGYPMAGHLAEAGIAMTVYNRTAQRAQQWTRDYPGSHAPSPADAARHADLVFICVGDDNDLRQVTLG
DDGVMQTLAKGAVLVDHTTASAEVARELDAASRKQGSHFLDAPVSGGQQGAQNGQLSIMCGGDEATYQRVLPTLQHYGKA
INLMGPAGSGQLTKMVNQICVAGLVEGLAEGLAFALNAGLDANKVFETISQGAAGSWQMVNRHQTMIDDQYEHGFAVDWM
RKDLDICLREAVRNGTPLPITEQINDFYREIQSMGGGRWDTSSLLRRLQKIPT

Sequences:

>Translated_293_residues
MSTLNAAFIGLGTMGYPMAGHLAEAGIAMTVYNRTAQRAQQWTRDYPGSHAPSPADAARHADLVFICVGDDNDLRQVTLG
DDGVMQTLAKGAVLVDHTTASAEVARELDAASRKQGSHFLDAPVSGGQQGAQNGQLSIMCGGDEATYQRVLPTLQHYGKA
INLMGPAGSGQLTKMVNQICVAGLVEGLAEGLAFALNAGLDANKVFETISQGAAGSWQMVNRHQTMIDDQYEHGFAVDWM
RKDLDICLREAVRNGTPLPITEQINDFYREIQSMGGGRWDTSSLLRRLQKIPT
>Mature_292_residues
STLNAAFIGLGTMGYPMAGHLAEAGIAMTVYNRTAQRAQQWTRDYPGSHAPSPADAARHADLVFICVGDDNDLRQVTLGD
DGVMQTLAKGAVLVDHTTASAEVARELDAASRKQGSHFLDAPVSGGQQGAQNGQLSIMCGGDEATYQRVLPTLQHYGKAI
NLMGPAGSGQLTKMVNQICVAGLVEGLAEGLAFALNAGLDANKVFETISQGAAGSWQMVNRHQTMIDDQYEHGFAVDWMR
KDLDICLREAVRNGTPLPITEQINDFYREIQSMGGGRWDTSSLLRRLQKIPT

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=28.3687943262411, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=28.2758620689655, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=34.3971631205674, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=30.1038062283737, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1790315, Length=287, Percent_Identity=28.9198606271777, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=27.7397260273973, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=26.6666666666667, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=27.9863481228669, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=27.9863481228669, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=22.7758007117438, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31506; Mature: 31375

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNAAFIGLGTMGYPMAGHLAEAGIAMTVYNRTAQRAQQWTRDYPGSHAPSPADAARH
CCCCHHHHHCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
ADLVFICVGDDNDLRQVTLGDDGVMQTLAKGAVLVDHTTASAEVARELDAASRKQGSHFL
CCEEEEEECCCCCCEEEEECCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEE
DAPVSGGQQGAQNGQLSIMCGGDEATYQRVLPTLQHYGKAINLMGPAGSGQLTKMVNQIC
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
VAGLVEGLAEGLAFALNAGLDANKVFETISQGAAGSWQMVNRHQTMIDDQYEHGFAVDWM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCHHHHH
RKDLDICLREAVRNGTPLPITEQINDFYREIQSMGGGRWDTSSLLRRLQKIPT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
STLNAAFIGLGTMGYPMAGHLAEAGIAMTVYNRTAQRAQQWTRDYPGSHAPSPADAARH
CCCHHHHHCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
ADLVFICVGDDNDLRQVTLGDDGVMQTLAKGAVLVDHTTASAEVARELDAASRKQGSHFL
CCEEEEEECCCCCCEEEEECCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEE
DAPVSGGQQGAQNGQLSIMCGGDEATYQRVLPTLQHYGKAINLMGPAGSGQLTKMVNQIC
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
VAGLVEGLAEGLAFALNAGLDANKVFETISQGAAGSWQMVNRHQTMIDDQYEHGFAVDWM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCHHHHH
RKDLDICLREAVRNGTPLPITEQINDFYREIQSMGGGRWDTSSLLRRLQKIPT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA