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The map label for this gene is xthA [H]

Identifier: 110834346

GI number: 110834346

Start: 1693134

End: 1693949

Strand: Direct

Name: xthA [H]

Synonym: ABO_1485

Alternate gene names: 110834346

Gene position: 1693134-1693949 (Clockwise)

Preceding gene: 110834340

Following gene: 110834347

Centisome position: 54.26

GC content: 53.43

Gene sequence:

>816_bases
ATGACCCTGATTTCATTCAACATCAATGGTATTCGTGCCCGTCTACATCAGCTCCAGGCGGTGATCGACAAATACCAACC
CGCATTAATCGGCTTGCAGGAAACCAAGGTTCACGACCCGGATTTTCCAGAGCAGGCGGTTCGAGATATGGGCTACCATC
CCTATTATTTTGGCCAGAAAGGCCACTATGGCGTGGCCCTGATTACCCGCGAACCTCTGGAATCAGTGCAGTATGGTTTC
CCCACCGACGACGAAGACGCGCAGCGGCGCATGATTATCGGCACACTGAAACATAATGATGGTAGCCTAATCACAGTACT
CAACGGCTACTTCCCTCAAGGGGAAAACCGCGACCACCCGTTAAAGTTTCCTGCCAAGAAAAAATTCTACGAAGATCTGC
AGCATTACCTGATCACCCACCATCAGCCCAGCGATCAAGTGGCGGTAATGGGGGACTTCAATATTTCATCTACAGATCTG
GATATCGGCATTGGCGAGCCGAACCGCAAGCGCTGGCTGCGCGAAGGCAAAACCAGCTTCCTGCCTGAAGAAAGGGAATG
GTGGGACCGCCTAGTAGGCTGGGGCCTGACTGACACCTTCCGACACCAGAATCCCACCACCGACGACGTGTTCTCCTGGT
TTGACTATCGCTCCAAGGGCTTTGATCGTGAGCCACGCCGAGGCCTGCGCATTGATACCGTATTGGCTACTGAACCACTG
ATGGCCAAGCTGGACACCACCGGTGTGGATTATCAGATGCGCGCAATGGAAAAGCCCTCGGATCATTGTCCGGTCTGGGC
ACGTTTTAACGGCTGA

Upstream 100 bases:

>100_bases
AAGCCTAAGGGGCTGGCTGGCACGCCAATCGTATCATTCTATCAAATCCGCGATGAACGACTTTTAAGGTAGTGTACAAT
GCGGGCACCAAGGAGGCGCT

Downstream 100 bases:

>100_bases
GCAGGTTATTCCATGAAAGTCAGGCTGCACCCGGAAAAGTTAAAAGCGAACCTGGATACCATCGGTTTCATTATCGACCT
GGCCATGATTGTGCTGGTGA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTLISFNINGIRARLHQLQAVIDKYQPALIGLQETKVHDPDFPEQAVRDMGYHPYYFGQKGHYGVALITREPLESVQYGF
PTDDEDAQRRMIIGTLKHNDGSLITVLNGYFPQGENRDHPLKFPAKKKFYEDLQHYLITHHQPSDQVAVMGDFNISSTDL
DIGIGEPNRKRWLREGKTSFLPEEREWWDRLVGWGLTDTFRHQNPTTDDVFSWFDYRSKGFDREPRRGLRIDTVLATEPL
MAKLDTTGVDYQMRAMEKPSDHCPVWARFNG

Sequences:

>Translated_271_residues
MTLISFNINGIRARLHQLQAVIDKYQPALIGLQETKVHDPDFPEQAVRDMGYHPYYFGQKGHYGVALITREPLESVQYGF
PTDDEDAQRRMIIGTLKHNDGSLITVLNGYFPQGENRDHPLKFPAKKKFYEDLQHYLITHHQPSDQVAVMGDFNISSTDL
DIGIGEPNRKRWLREGKTSFLPEEREWWDRLVGWGLTDTFRHQNPTTDDVFSWFDYRSKGFDREPRRGLRIDTVLATEPL
MAKLDTTGVDYQMRAMEKPSDHCPVWARFNG
>Mature_270_residues
TLISFNINGIRARLHQLQAVIDKYQPALIGLQETKVHDPDFPEQAVRDMGYHPYYFGQKGHYGVALITREPLESVQYGFP
TDDEDAQRRMIIGTLKHNDGSLITVLNGYFPQGENRDHPLKFPAKKKFYEDLQHYLITHHQPSDQVAVMGDFNISSTDLD
IGIGEPNRKRWLREGKTSFLPEEREWWDRLVGWGLTDTFRHQNPTTDDVFSWFDYRSKGFDREPRRGLRIDTVLATEPLM
AKLDTTGVDYQMRAMEKPSDHCPVWARFNG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=63.9405204460966, Blast_Score=360, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=29.6703296703297, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=29.6703296703297, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31485; Mature: 31354

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLISFNINGIRARLHQLQAVIDKYQPALIGLQETKVHDPDFPEQAVRDMGYHPYYFGQK
CEEEEECCHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHHHHCCCCCEEECCC
GHYGVALITREPLESVQYGFPTDDEDAQRRMIIGTLKHNDGSLITVLNGYFPQGENRDHP
CCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
LKFPAKKKFYEDLQHYLITHHQPSDQVAVMGDFNISSTDLDIGIGEPNRKRWLREGKTSF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCC
LPEEREWWDRLVGWGLTDTFRHQNPTTDDVFSWFDYRSKGFDREPRRGLRIDTVLATEPL
CCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCH
MAKLDTTGVDYQMRAMEKPSDHCPVWARFNG
HHHHCCCCCCEEEHHCCCCCCCCCEEEEECC
>Mature Secondary Structure 
TLISFNINGIRARLHQLQAVIDKYQPALIGLQETKVHDPDFPEQAVRDMGYHPYYFGQK
EEEEECCHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHHHHCCCCCEEECCC
GHYGVALITREPLESVQYGFPTDDEDAQRRMIIGTLKHNDGSLITVLNGYFPQGENRDHP
CCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
LKFPAKKKFYEDLQHYLITHHQPSDQVAVMGDFNISSTDLDIGIGEPNRKRWLREGKTSF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCC
LPEEREWWDRLVGWGLTDTFRHQNPTTDDVFSWFDYRSKGFDREPRRGLRIDTVLATEPL
CCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCH
MAKLDTTGVDYQMRAMEKPSDHCPVWARFNG
HHHHCCCCCCEEEHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]