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The map label for this gene is alkA [H]

Identifier: 110834274

GI number: 110834274

Start: 1611317

End: 1612192

Strand: Direct

Name: alkA [H]

Synonym: ABO_1413

Alternate gene names: 110834274

Gene position: 1611317-1612192 (Clockwise)

Preceding gene: 110834273

Following gene: 110834275

Centisome position: 51.64

GC content: 57.53

Gene sequence:

>876_bases
ATGACAACGGTAAAGCTGCCACTACCCGCCCACTTTAGCGTTTCCGACTTTCTTCATTTTCATGGTCGAGACAGTCATGC
CGTTTCCGAACGGGTAGATGCCCTTACGCTGCACAAAGCCATTATCTTTAACCTCCGGCCCTGTATCCTGAAAATGGACT
TCAACCAAGTCGGGCACGTGATTACTAACGCCACAGACACCACCGCCCCGGCCTTGACCCGCCAAGCCAGCGCCATGCTG
GGCCTGAATCAACCTGTGGAGGTATTCGAAACGGCGATAGGCACAAAGCCCCCTTTGAATACTTTGGTGCAACGACAAAG
AGGGCTGCGGGTGCCGCAATCGGCAACGCCGTTCGAGGCGCTTACCTGGGCCATTATTGGCCAACAGATTAGCGTGAGTG
CGGCCACCGCTATTCGCCGCCGTTTTATCCAGCTGGCGAGCCCGGTTCGACACGATGGCCTTCACTGCTACCCGGATGCC
GCCACAGTCTGCTTGCTGACGCCAGACGCTCTTCGCAGCGTGGGATTTTCCGCCACCAAAGCTGACACTTTACTGGCCGT
GTCCCGGCTCTGTCGCGACCAGCAACTTTTGCCAGAAACATTGCATCTCGACGCTTACGCGGAGCAACTGGAGCGCAACC
TGCTTGAGATTCGTGGCCTGGGACCCTGGAGCGTGAATTACACCCTGCTAAGAGGCTACGGTTTTCTGGACGGAAGCCTG
CATGCCGACGTGGCGGTACAAAAGGCGCTACAAATGTTGCTTGGGCAACCGGAACGTCCCACGGCCAGAGTAACCCGGGA
TTGGTTGGCAGACTTCACGCCCTGGCGGGCACTGGTGGCCGCACACCTGTGGCAATCCTTATCGACACAAGCGTGA

Upstream 100 bases:

>100_bases
GTCATCGTATTGTGCGCACTGATGGCGGGCTGTCTGGCTACCGCTGGGGTGTTGGCCGCAAAGCCGCTTTACTCGACCGC
GAACGGGAGCCCAGCGACAA

Downstream 100 bases:

>100_bases
AGGCACTTTCTAGTGTCCCGGATTAGACCTTGCGAGCCAGCCGCCATGCCAGAATCGACATGGGCGCCAGTAGCGTAATC
ACTAACAGGTACGGCCAGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTTVKLPLPAHFSVSDFLHFHGRDSHAVSERVDALTLHKAIIFNLRPCILKMDFNQVGHVITNATDTTAPALTRQASAML
GLNQPVEVFETAIGTKPPLNTLVQRQRGLRVPQSATPFEALTWAIIGQQISVSAATAIRRRFIQLASPVRHDGLHCYPDA
ATVCLLTPDALRSVGFSATKADTLLAVSRLCRDQQLLPETLHLDAYAEQLERNLLEIRGLGPWSVNYTLLRGYGFLDGSL
HADVAVQKALQMLLGQPERPTARVTRDWLADFTPWRALVAAHLWQSLSTQA

Sequences:

>Translated_291_residues
MTTVKLPLPAHFSVSDFLHFHGRDSHAVSERVDALTLHKAIIFNLRPCILKMDFNQVGHVITNATDTTAPALTRQASAML
GLNQPVEVFETAIGTKPPLNTLVQRQRGLRVPQSATPFEALTWAIIGQQISVSAATAIRRRFIQLASPVRHDGLHCYPDA
ATVCLLTPDALRSVGFSATKADTLLAVSRLCRDQQLLPETLHLDAYAEQLERNLLEIRGLGPWSVNYTLLRGYGFLDGSL
HADVAVQKALQMLLGQPERPTARVTRDWLADFTPWRALVAAHLWQSLSTQA
>Mature_290_residues
TTVKLPLPAHFSVSDFLHFHGRDSHAVSERVDALTLHKAIIFNLRPCILKMDFNQVGHVITNATDTTAPALTRQASAMLG
LNQPVEVFETAIGTKPPLNTLVQRQRGLRVPQSATPFEALTWAIIGQQISVSAATAIRRRFIQLASPVRHDGLHCYPDAA
TVCLLTPDALRSVGFSATKADTLLAVSRLCRDQQLLPETLHLDAYAEQLERNLLEIRGLGPWSVNYTLLRGYGFLDGSLH
ADVAVQKALQMLLGQPERPTARVTRDWLADFTPWRALVAAHLWQSLSTQA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=194, Percent_Identity=27.8350515463918, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 32134; Mature: 32003

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVKLPLPAHFSVSDFLHFHGRDSHAVSERVDALTLHKAIIFNLRPCILKMDFNQVGHV
CCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEECHHHHHHH
ITNATDTTAPALTRQASAMLGLNQPVEVFETAIGTKPPLNTLVQRQRGLRVPQSATPFEA
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHH
LTWAIIGQQISVSAATAIRRRFIQLASPVRHDGLHCYPDAATVCLLTPDALRSVGFSATK
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCCEEEEECHHHHHHCCCCHHH
ADTLLAVSRLCRDQQLLPETLHLDAYAEQLERNLLEIRGLGPWSVNYTLLRGYGFLDGSL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCH
HADVAVQKALQMLLGQPERPTARVTRDWLADFTPWRALVAAHLWQSLSTQA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTVKLPLPAHFSVSDFLHFHGRDSHAVSERVDALTLHKAIIFNLRPCILKMDFNQVGHV
CEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEECHHHHHHH
ITNATDTTAPALTRQASAMLGLNQPVEVFETAIGTKPPLNTLVQRQRGLRVPQSATPFEA
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHH
LTWAIIGQQISVSAATAIRRRFIQLASPVRHDGLHCYPDAATVCLLTPDALRSVGFSATK
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCCEEEEECHHHHHHCCCCHHH
ADTLLAVSRLCRDQQLLPETLHLDAYAEQLERNLLEIRGLGPWSVNYTLLRGYGFLDGSL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCH
HADVAVQKALQMLLGQPERPTARVTRDWLADFTPWRALVAAHLWQSLSTQA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]