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The map label for this gene is ada [H]

Identifier: 110834273

GI number: 110834273

Start: 1610256

End: 1611320

Strand: Direct

Name: ada [H]

Synonym: ABO_1412

Alternate gene names: 110834273

Gene position: 1610256-1611320 (Clockwise)

Preceding gene: 110834272

Following gene: 110834274

Centisome position: 51.61

GC content: 56.62

Gene sequence:

>1065_bases
ATGAGCACCGAGCAACAACGGCGACAAGCCATCAAAGACCACTCCCCCTTGCCGGGAGATAACTTTGTTTATGGTGTGCT
GACGACGGGAATTTTCTGCTTGCCTATCTGTCCATCGCGACGTCCTCGCGATGAAAATGTTCGTCTCTTCGACACCCCCG
TACAGGCTATTCAGAATGGCTACCGACCCTGTCGGCGCTGCCATCCACTTGGAGATATCGCCGCACCATCAACAGCCACA
GTGATTGCGCTATGTCGATTGATTGAAGCGGCCGAACGAAAACCCACGCTCACGGAGTTATCGGCGCAATCGGGCTGGAG
CGCTCATCATCTGCAACGCCAATTCAAAGCGGTTGCCGGCATTTCACCGCACCAGTACGGTGTCGCCATTCGCCGCCGGC
GAGCGCAACAGCAGCTCCGCTATAAACAGTCATTGACCCTCTCGGCTCACTTGGCCGGATTTGACTCGACTAGCCACCTA
CATGCCGTCGCTGGCAACACCCTTGGCATGAAACCAGGCCAATATCGACAAGCAGGTAAAGATATGGCACTTCGATTTGC
CATCGCCGAAAGCTCACTCGGCTCTGTGCTGGTGGCAGAAAGCGAGCGTGGCATCTGTGCCGTCACCATAGGGGATGATC
CCAGCGTGTTACTGCAGGATTTCGAACAGGAATTCAGTGGCGCCACCCTGCTGCCGGGTAATAGTGAGTTTGATCAGCGC
GTTGCCATGGTCATTACCCTAATCGACGACCCGCATCAGATTACTCAAGCACCTCGTGATCTGCTGCCTTTGGATATTCG
AGGGACCGCCTTCCAGCAACAAGTCTGGGCGGCACTACAGACGATTCCCACAGGCCAGACCCTGTCTTATCAGGCACTGG
CAGTAAAGCTTGGCAAGCCTACAGCCGCCCGAGCTGTTGCCCAGGCCTGCGCCAGTAACCGGCTGGCTGTTTTGGTTCCT
TGTCATCGTATTGTGCGCACTGATGGCGGGCTGTCTGGCTACCGCTGGGGTGTTGGCCGCAAAGCCGCTTTACTCGACCG
CGAACGGGAGCCCAGCGACAAATGA

Upstream 100 bases:

>100_bases
TTTGGATGCTCACTCACCCTGGTTTCCGCGATGCTCCCAGTCAGTCTCATTTCCCTTCATACTGCGGGTAGCACATATGG
AAACAGGTCTGAATACAACC

Downstream 100 bases:

>100_bases
CAACGGTAAAGCTGCCACTACCCGCCCACTTTAGCGTTTCCGACTTTCTTCATTTTCATGGTCGAGACAGTCATGCCGTT
TCCGAACGGGTAGATGCCCT

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSTEQQRRQAIKDHSPLPGDNFVYGVLTTGIFCLPICPSRRPRDENVRLFDTPVQAIQNGYRPCRRCHPLGDIAAPSTAT
VIALCRLIEAAERKPTLTELSAQSGWSAHHLQRQFKAVAGISPHQYGVAIRRRRAQQQLRYKQSLTLSAHLAGFDSTSHL
HAVAGNTLGMKPGQYRQAGKDMALRFAIAESSLGSVLVAESERGICAVTIGDDPSVLLQDFEQEFSGATLLPGNSEFDQR
VAMVITLIDDPHQITQAPRDLLPLDIRGTAFQQQVWAALQTIPTGQTLSYQALAVKLGKPTAARAVAQACASNRLAVLVP
CHRIVRTDGGLSGYRWGVGRKAALLDREREPSDK

Sequences:

>Translated_354_residues
MSTEQQRRQAIKDHSPLPGDNFVYGVLTTGIFCLPICPSRRPRDENVRLFDTPVQAIQNGYRPCRRCHPLGDIAAPSTAT
VIALCRLIEAAERKPTLTELSAQSGWSAHHLQRQFKAVAGISPHQYGVAIRRRRAQQQLRYKQSLTLSAHLAGFDSTSHL
HAVAGNTLGMKPGQYRQAGKDMALRFAIAESSLGSVLVAESERGICAVTIGDDPSVLLQDFEQEFSGATLLPGNSEFDQR
VAMVITLIDDPHQITQAPRDLLPLDIRGTAFQQQVWAALQTIPTGQTLSYQALAVKLGKPTAARAVAQACASNRLAVLVP
CHRIVRTDGGLSGYRWGVGRKAALLDREREPSDK
>Mature_353_residues
STEQQRRQAIKDHSPLPGDNFVYGVLTTGIFCLPICPSRRPRDENVRLFDTPVQAIQNGYRPCRRCHPLGDIAAPSTATV
IALCRLIEAAERKPTLTELSAQSGWSAHHLQRQFKAVAGISPHQYGVAIRRRRAQQQLRYKQSLTLSAHLAGFDSTSHLH
AVAGNTLGMKPGQYRQAGKDMALRFAIAESSLGSVLVAESERGICAVTIGDDPSVLLQDFEQEFSGATLLPGNSEFDQRV
AMVITLIDDPHQITQAPRDLLPLDIRGTAFQQQVWAALQTIPTGQTLSYQALAVKLGKPTAARAVAQACASNRLAVLVPC
HRIVRTDGGLSGYRWGVGRKAALLDREREPSDK

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788542, Length=356, Percent_Identity=46.3483146067416, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1787596, Length=89, Percent_Identity=49.438202247191, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=166, Percent_Identity=33.1325301204819, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=131, Percent_Identity=35.8778625954198, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=96, Percent_Identity=45.8333333333333, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137554, Length=88, Percent_Identity=48.8636363636364, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38686; Mature: 38554

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEQQRRQAIKDHSPLPGDNFVYGVLTTGIFCLPICPSRRPRDENVRLFDTPVQAIQNG
CCCHHHHHHHHHCCCCCCCCCEEHHHHHHCEEEEECCCCCCCCCCCCEEECCHHHHHHHC
YRPCRRCHPLGDIAAPSTATVIALCRLIEAAERKPTLTELSAQSGWSAHHLQRQFKAVAG
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHC
ISPHQYGVAIRRRRAQQQLRYKQSLTLSAHLAGFDSTSHLHAVAGNTLGMKPGQYRQAGK
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCC
DMALRFAIAESSLGSVLVAESERGICAVTIGDDPSVLLQDFEQEFSGATLLPGNSEFDQR
CCEEEEEEECCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCHHHHE
VAMVITLIDDPHQITQAPRDLLPLDIRGTAFQQQVWAALQTIPTGQTLSYQALAVKLGKP
EEEEEEECCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
TAARAVAQACASNRLAVLVPCHRIVRTDGGLSGYRWGVGRKAALLDREREPSDK
HHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEECCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
STEQQRRQAIKDHSPLPGDNFVYGVLTTGIFCLPICPSRRPRDENVRLFDTPVQAIQNG
CCHHHHHHHHHCCCCCCCCCEEHHHHHHCEEEEECCCCCCCCCCCCEEECCHHHHHHHC
YRPCRRCHPLGDIAAPSTATVIALCRLIEAAERKPTLTELSAQSGWSAHHLQRQFKAVAG
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHC
ISPHQYGVAIRRRRAQQQLRYKQSLTLSAHLAGFDSTSHLHAVAGNTLGMKPGQYRQAGK
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCC
DMALRFAIAESSLGSVLVAESERGICAVTIGDDPSVLLQDFEQEFSGATLLPGNSEFDQR
CCEEEEEEECCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCHHHHE
VAMVITLIDDPHQITQAPRDLLPLDIRGTAFQQQVWAALQTIPTGQTLSYQALAVKLGKP
EEEEEEECCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
TAARAVAQACASNRLAVLVPCHRIVRTDGGLSGYRWGVGRKAALLDREREPSDK
HHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEECCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]