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The map label for this gene is rlmI [H]

Identifier: 110834042

GI number: 110834042

Start: 1341727

End: 1342701

Strand: Direct

Name: rlmI [H]

Synonym: ABO_1181

Alternate gene names: 110834042

Gene position: 1341727-1342701 (Clockwise)

Preceding gene: 110834040

Following gene: 110834045

Centisome position: 43.0

GC content: 50.77

Gene sequence:

>975_bases
TTGTCTCTCTTACTGCTTGTATTGATCCCTATGCTCTCGACCCAGCTATCAGACCTGCTTGCTCCGTATCTGGTTCCATT
GTCTCAACCAAAACATCGCCTTTTTCATGGTCGCGGTAAAACGGTGCCATCCCTGGAATGGCTGACGATAGATTGCTTTC
CTCCTCTGCTGGTGGCCACTCTGTTTCGCGCACAGGAAGCGGAGATGTTGGCGGCGCTGGAGGTTTATTTAAGCGAAACC
ATGGCGTCACTGGGCTGCGATGTGCTGGTTTTTCAACATCGTTATGACGGCAAGGCCGATAATGTTGTCGTGAAGGGTGA
AATGCCCTCTCACTCTGTGGCCACAGAGCAAGGGCTGGTTTTCAAACTGGATTTTCAGCGCGGCCAGAACTTGGGATTCT
TTGCCGATATGGCTCAAGGCCGGCGCTGGATACGCGAACGGGCTGACGGCAAGAAGGTGCTCAATCTGTTCAGTTTTACG
TGTAGTTTTTCTGTGGCTGCACTAGCAGGTGGAGCCCATTCCGTGGTGAATATTGATTTATCTGATGCGGCGCTGACCCT
AGGTAAACAAAACCATGCGTTGAATGGTTTCCAGGACGCCCGAGTTCACTATTTACCGCACAATATATTTCGGAGTTGGA
AAAAACTGAATCAGTTGGGCCGCTATGAATTGATTGTGATTGATCCCCCCAGCGCCCAGCGTGGCAGCTTTATGGCGCAA
GAGGATTACCCAAGAGTGCTGCGCAAGCTGCATAAATTGTTGGCGGTAGAAGCAGAGTTACTGGTCTGCCTCAATGCCCC
TTGGCTGGATTATGATTGGCTGGAACAGTGCGTGGAAAAAAATCTTCCCGGTGCAGAAAAAGTCTCTAGATTGCCGGGGG
CGATCGGTTTTGATGAAGTCGGTGAGCCGGCGTTAAAGACTGTGCATTATCGTTATCGTCGCCCTCAGGCTTTGGATGAA
GGTAATACTCCGTAG

Upstream 100 bases:

>100_bases
GCCTTGTCTAGGCATGTAAGGCGTTATTCAAAGACTCCCCTGAGCTATAATTAGCCCTTGTCAGCTGGAGAGAAGCATCC
TGTGTTTCTGCCAGCATGCT

Downstream 100 bases:

>100_bases
TCACTCAAGTTTGCTGGTTGGCAACAGCGGGCAACCCCTTTATGCACTCTGGCCGGTACTTTTGTCGCCGCGCAGTTAAC
GCTCGCCCTGGTTACAGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSLLLLVLIPMLSTQLSDLLAPYLVPLSQPKHRLFHGRGKTVPSLEWLTIDCFPPLLVATLFRAQEAEMLAALEVYLSET
MASLGCDVLVFQHRYDGKADNVVVKGEMPSHSVATEQGLVFKLDFQRGQNLGFFADMAQGRRWIRERADGKKVLNLFSFT
CSFSVAALAGGAHSVVNIDLSDAALTLGKQNHALNGFQDARVHYLPHNIFRSWKKLNQLGRYELIVIDPPSAQRGSFMAQ
EDYPRVLRKLHKLLAVEAELLVCLNAPWLDYDWLEQCVEKNLPGAEKVSRLPGAIGFDEVGEPALKTVHYRYRRPQALDE
GNTP

Sequences:

>Translated_324_residues
MSLLLLVLIPMLSTQLSDLLAPYLVPLSQPKHRLFHGRGKTVPSLEWLTIDCFPPLLVATLFRAQEAEMLAALEVYLSET
MASLGCDVLVFQHRYDGKADNVVVKGEMPSHSVATEQGLVFKLDFQRGQNLGFFADMAQGRRWIRERADGKKVLNLFSFT
CSFSVAALAGGAHSVVNIDLSDAALTLGKQNHALNGFQDARVHYLPHNIFRSWKKLNQLGRYELIVIDPPSAQRGSFMAQ
EDYPRVLRKLHKLLAVEAELLVCLNAPWLDYDWLEQCVEKNLPGAEKVSRLPGAIGFDEVGEPALKTVHYRYRRPQALDE
GNTP
>Mature_323_residues
SLLLLVLIPMLSTQLSDLLAPYLVPLSQPKHRLFHGRGKTVPSLEWLTIDCFPPLLVATLFRAQEAEMLAALEVYLSETM
ASLGCDVLVFQHRYDGKADNVVVKGEMPSHSVATEQGLVFKLDFQRGQNLGFFADMAQGRRWIRERADGKKVLNLFSFTC
SFSVAALAGGAHSVVNIDLSDAALTLGKQNHALNGFQDARVHYLPHNIFRSWKKLNQLGRYELIVIDPPSAQRGSFMAQE
DYPRVLRKLHKLLAVEAELLVCLNAPWLDYDWLEQCVEKNLPGAEKVSRLPGAIGFDEVGEPALKTVHYRYRRPQALDEG
NTP

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=205, Percent_Identity=30.7317073170732, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787181, Length=229, Percent_Identity=27.0742358078603, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 36293; Mature: 36162

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLLLVLIPMLSTQLSDLLAPYLVPLSQPKHRLFHGRGKTVPSLEWLTIDCFPPLLVAT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEECCHHHHHHH
LFRAQEAEMLAALEVYLSETMASLGCDVLVFQHRYDGKADNVVVKGEMPSHSVATEQGLV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCE
FKLDFQRGQNLGFFADMAQGRRWIRERADGKKVLNLFSFTCSFSVAALAGGAHSVVNIDL
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SDAALTLGKQNHALNGFQDARVHYLPHNIFRSWKKLNQLGRYELIVIDPPSAQRGSFMAQ
CHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
EDYPRVLRKLHKLLAVEAELLVCLNAPWLDYDWLEQCVEKNLPGAEKVSRLPGAIGFDEV
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHC
GEPALKTVHYRYRRPQALDEGNTP
CCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SLLLLVLIPMLSTQLSDLLAPYLVPLSQPKHRLFHGRGKTVPSLEWLTIDCFPPLLVAT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEECCHHHHHHH
LFRAQEAEMLAALEVYLSETMASLGCDVLVFQHRYDGKADNVVVKGEMPSHSVATEQGLV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCE
FKLDFQRGQNLGFFADMAQGRRWIRERADGKKVLNLFSFTCSFSVAALAGGAHSVVNIDL
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SDAALTLGKQNHALNGFQDARVHYLPHNIFRSWKKLNQLGRYELIVIDPPSAQRGSFMAQ
CHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
EDYPRVLRKLHKLLAVEAELLVCLNAPWLDYDWLEQCVEKNLPGAEKVSRLPGAIGFDEV
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHC
GEPALKTVHYRYRRPQALDEGNTP
CCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA