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The map label for this gene is rsuA [H]

Identifier: 110833959

GI number: 110833959

Start: 1246547

End: 1247293

Strand: Direct

Name: rsuA [H]

Synonym: ABO_1098

Alternate gene names: 110833959

Gene position: 1246547-1247293 (Clockwise)

Preceding gene: 110833958

Following gene: 110833962

Centisome position: 39.95

GC content: 56.63

Gene sequence:

>747_bases
GTGCTCGGTTATCATGTGTTGATGACTTCTCGACACCATCGCCTAGACCGTTTTCTCAGCGCCCGACTGCAAATGAGCCG
AAGCAATCTGCGGCTGTTGCTGGCTGCCGGGCGCGTGCGGGTAAACGGGCAGGTGGAGCGGGATCGGCAGCGGCGAATTG
GCCTTTTTGATGCGGTGGTGTGCGATGGTGAAATTCTGCAGGCGAACCGGCCGCGTTATTTGATGATTAACAAGCCTGCG
GGGGTCGTCAGCGCTACCCGAGATGAGCACCACCCCACGGTGCTGGAGCTGCTGCCAAGGGGCCAGCGTGAAGGGCTGCA
TCTGGCCGGGCGGTTGGATTTTAATTCCACAGGGTTGGTGTTGCTGACCAATGATGGTGCCTGGTCGCGTCGCTTGAGCT
TGCCGGAAACGGGCCTCTGGAAACATTACCGGGTGAGTGTTGATAAACCGTTGAGCGATGAAGATGTAGTGTCATTTTCG
CAACGTATGTGGTTCGACTTCGAGCAAATTTACACCCAGCCGGCCAGGCTAACGATTCGTTCTTCTTTTGAAGCGGATGT
ATGGCTTCACGAAGGCAAGTATCACCAGATTAAGCGAATGTTTGGGCGTACTGGGAAGAAGGTAACGGCGCTGCACCGTT
TGGCCGTAGGCCGCATTCAGTTGGACGCTTACCTGGCACCGGGGGAAAGCCGGGAGCTGACCCAGGCAGAGCAGCAAACG
GCTTTAGATCCAGAGCCAGTGGAGTAA

Upstream 100 bases:

>100_bases
GCCGTAACAGCGCAGAGGACAGCGTGAGAAAAGTGTAAGAGTGATGCTTGATTGAGCTTGCCAGCCATGGGTATGTCGCT
TCCGGCTACGGGAAATCTGT

Downstream 100 bases:

>100_bases
AGTGCGGTTGTCTTTGGCTTGAGCCGTGAGTGTGTTTCGCCTGCATTGTCTTGAGGTTGTGTCAGGGCAGGAGGTTGCTG
TTAGCGTCGCCGCGCGCGGA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLGYHVLMTSRHHRLDRFLSARLQMSRSNLRLLLAAGRVRVNGQVERDRQRRIGLFDAVVCDGEILQANRPRYLMINKPA
GVVSATRDEHHPTVLELLPRGQREGLHLAGRLDFNSTGLVLLTNDGAWSRRLSLPETGLWKHYRVSVDKPLSDEDVVSFS
QRMWFDFEQIYTQPARLTIRSSFEADVWLHEGKYHQIKRMFGRTGKKVTALHRLAVGRIQLDAYLAPGESRELTQAEQQT
ALDPEPVE

Sequences:

>Translated_248_residues
MLGYHVLMTSRHHRLDRFLSARLQMSRSNLRLLLAAGRVRVNGQVERDRQRRIGLFDAVVCDGEILQANRPRYLMINKPA
GVVSATRDEHHPTVLELLPRGQREGLHLAGRLDFNSTGLVLLTNDGAWSRRLSLPETGLWKHYRVSVDKPLSDEDVVSFS
QRMWFDFEQIYTQPARLTIRSSFEADVWLHEGKYHQIKRMFGRTGKKVTALHRLAVGRIQLDAYLAPGESRELTQAEQQT
ALDPEPVE
>Mature_248_residues
MLGYHVLMTSRHHRLDRFLSARLQMSRSNLRLLLAAGRVRVNGQVERDRQRRIGLFDAVVCDGEILQANRPRYLMINKPA
GVVSATRDEHHPTVLELLPRGQREGLHLAGRLDFNSTGLVLLTNDGAWSRRLSLPETGLWKHYRVSVDKPLSDEDVVSFS
QRMWFDFEQIYTQPARLTIRSSFEADVWLHEGKYHQIKRMFGRTGKKVTALHRLAVGRIQLDAYLAPGESRELTQAEQQT
ALDPEPVE

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=225, Percent_Identity=40.8888888888889, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=27.6315789473684, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=28.4518828451883, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28486; Mature: 28486

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGYHVLMTSRHHRLDRFLSARLQMSRSNLRLLLAAGRVRVNGQVERDRQRRIGLFDAVV
CCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCHHHHCCHHEEEE
CDGEILQANRPRYLMINKPAGVVSATRDEHHPTVLELLPRGQREGLHLAGRLDFNSTGLV
ECCCEEECCCCCEEEEECCCCCEECCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCEE
LLTNDGAWSRRLSLPETGLWKHYRVSVDKPLSDEDVVSFSQRMWFDFEQIYTQPARLTIR
EEECCCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEE
SSFEADVWLHEGKYHQIKRMFGRTGKKVTALHRLAVGRIQLDAYLAPGESRELTQAEQQT
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHH
ALDPEPVE
CCCCCCCC
>Mature Secondary Structure
MLGYHVLMTSRHHRLDRFLSARLQMSRSNLRLLLAAGRVRVNGQVERDRQRRIGLFDAVV
CCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCHHHHCCHHEEEE
CDGEILQANRPRYLMINKPAGVVSATRDEHHPTVLELLPRGQREGLHLAGRLDFNSTGLV
ECCCEEECCCCCEEEEECCCCCEECCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCEE
LLTNDGAWSRRLSLPETGLWKHYRVSVDKPLSDEDVVSFSQRMWFDFEQIYTQPARLTIR
EEECCCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEE
SSFEADVWLHEGKYHQIKRMFGRTGKKVTALHRLAVGRIQLDAYLAPGESRELTQAEQQT
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHH
ALDPEPVE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]