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The map label for this gene is holB [H]

Identifier: 110833936

GI number: 110833936

Start: 1216742

End: 1217749

Strand: Direct

Name: holB [H]

Synonym: ABO_1075

Alternate gene names: 110833936

Gene position: 1216742-1217749 (Clockwise)

Preceding gene: 110833935

Following gene: 110833937

Centisome position: 39.0

GC content: 57.64

Gene sequence:

>1008_bases
ATGGCCGTAACCGGTGCAAAAGTTCAGGTCCCTTGCCCTTGGCACAGCGATGCTATGGCGCAGCTGCTTGATCAACACGC
CCGGGGGCGGCTGCCCCATGCCCTGTTGCTAAGCGGTCCGAAAGGCATTGGTAAATTGCGGCTGGCGGAGTCTTTGGCGC
AAACTTTATTATGTGAAAAACCCTCCGCTGGCTTGCCCTGTGGTCATTGCCAAGGTTGCCAGCTCAGCACGGCAGGCACC
CATCCAGATTTGTATCGACTTAGCCCGGAAGAAAAAAGCAAAACCATCAAGATTGACCAGGTGCGCAAGTTGGTAGCATT
TGCCACCCGTACTGCGCAATACAGTGGTTATCGTGTGGCGATTATTTCCCCCGCTGAAGCCCTTAACCGCAACGCCCAGA
ATGCGTTGCTGAAAACCCTCGAAGAGCCTGGAACACAAACCCTATTGTTGCTGGTTAGCGACCAACCAACCCTGCTGTTG
CCAACCATTCGTAGCCGTTGTCAGCAACGTATCTTGCCCTTACCGGTAAAGGAACATGCGTTACAGTGGCTGCAGTCGCA
AGTGGGAGAAAGCGCGGCCTCGTTACTGGATGCCGCTCAGGGGGCGCCTTTGCGGGCACTGGCTCTTGAGCAAGCGGAGT
GGTTCGCCGATCGGACCCGTTTGCTGGATACCCTAGTCAGGGTGGCAGAAGGGCGCCAATCGGTAGCGCAGGCCTCCCCG
GTGCTGGCCAAGCATGACCCCACGGAAATGGCTAGTGTGATTTACGGATGGCTATCGCGGAGTTTGAATCAACGTTTTTC
TGGTCAGCAGAGCTCCGATGCCTTGCTGGCGCCGCTGCTAGAGCGCCTTAATAAGGCTGTGCCTCCGGCTCGTCTGTTAC
GGGCCGCCCAGCGTGTGCTGGAAGGTCGCCGCGCCTTGTTGTCTGGAGCCAACCCCAATAAGGAGCTGCTCTACGAGCAG
TGGTTGTTGGTGTTGGTGGGCGTTGACGCTGCGGCGGCGGCGCACTAA

Upstream 100 bases:

>100_bases
GGGCAGCCCAGGAGCCCAATCGGTTTCGCACGGTAGACGCTAGCCAGCCGCTGGAATCAGTACAGCAACAACTAGCCCGG
ATTGTTGAGGAGATGCAGGC

Downstream 100 bases:

>100_bases
CCTTGATTATAGATTAATAATAATTTCGTAAGGGGTTGGTTCATGAAGATGCCGGTAGGTCGCAATGGCATTCTGTCGCT
TTCCATCAAAGATAAAGCGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAVTGAKVQVPCPWHSDAMAQLLDQHARGRLPHALLLSGPKGIGKLRLAESLAQTLLCEKPSAGLPCGHCQGCQLSTAGT
HPDLYRLSPEEKSKTIKIDQVRKLVAFATRTAQYSGYRVAIISPAEALNRNAQNALLKTLEEPGTQTLLLLVSDQPTLLL
PTIRSRCQQRILPLPVKEHALQWLQSQVGESAASLLDAAQGAPLRALALEQAEWFADRTRLLDTLVRVAEGRQSVAQASP
VLAKHDPTEMASVIYGWLSRSLNQRFSGQQSSDALLAPLLERLNKAVPPARLLRAAQRVLEGRRALLSGANPNKELLYEQ
WLLVLVGVDAAAAAH

Sequences:

>Translated_335_residues
MAVTGAKVQVPCPWHSDAMAQLLDQHARGRLPHALLLSGPKGIGKLRLAESLAQTLLCEKPSAGLPCGHCQGCQLSTAGT
HPDLYRLSPEEKSKTIKIDQVRKLVAFATRTAQYSGYRVAIISPAEALNRNAQNALLKTLEEPGTQTLLLLVSDQPTLLL
PTIRSRCQQRILPLPVKEHALQWLQSQVGESAASLLDAAQGAPLRALALEQAEWFADRTRLLDTLVRVAEGRQSVAQASP
VLAKHDPTEMASVIYGWLSRSLNQRFSGQQSSDALLAPLLERLNKAVPPARLLRAAQRVLEGRRALLSGANPNKELLYEQ
WLLVLVGVDAAAAAH
>Mature_334_residues
AVTGAKVQVPCPWHSDAMAQLLDQHARGRLPHALLLSGPKGIGKLRLAESLAQTLLCEKPSAGLPCGHCQGCQLSTAGTH
PDLYRLSPEEKSKTIKIDQVRKLVAFATRTAQYSGYRVAIISPAEALNRNAQNALLKTLEEPGTQTLLLLVSDQPTLLLP
TIRSRCQQRILPLPVKEHALQWLQSQVGESAASLLDAAQGAPLRALALEQAEWFADRTRLLDTLVRVAEGRQSVAQASPV
LAKHDPTEMASVIYGWLSRSLNQRFSGQQSSDALLAPLLERLNKAVPPARLLRAAQRVLEGRRALLSGANPNKELLYEQW
LLVLVGVDAAAAAH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=318, Percent_Identity=32.3899371069182, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786676, Length=141, Percent_Identity=36.1702127659575, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36345; Mature: 36214

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTGAKVQVPCPWHSDAMAQLLDQHARGRLPHALLLSGPKGIGKLRLAESLAQTLLCEK
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHCC
PSAGLPCGHCQGCQLSTAGTHPDLYRLSPEEKSKTIKIDQVRKLVAFATRTAQYSGYRVA
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCEEHHHHHHHHHHHHHHHHCCCEEEE
IISPAEALNRNAQNALLKTLEEPGTQTLLLLVSDQPTLLLPTIRSRCQQRILPLPVKEHA
EECCHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCEECHHHHHHHHHCCCCCCHHHHH
LQWLQSQVGESAASLLDAAQGAPLRALALEQAEWFADRTRLLDTLVRVAEGRQSVAQASP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLAKHDPTEMASVIYGWLSRSLNQRFSGQQSSDALLAPLLERLNKAVPPARLLRAAQRVL
CHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
EGRRALLSGANPNKELLYEQWLLVLVGVDAAAAAH
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
AVTGAKVQVPCPWHSDAMAQLLDQHARGRLPHALLLSGPKGIGKLRLAESLAQTLLCEK
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHCC
PSAGLPCGHCQGCQLSTAGTHPDLYRLSPEEKSKTIKIDQVRKLVAFATRTAQYSGYRVA
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCEEHHHHHHHHHHHHHHHHCCCEEEE
IISPAEALNRNAQNALLKTLEEPGTQTLLLLVSDQPTLLLPTIRSRCQQRILPLPVKEHA
EECCHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCEECHHHHHHHHHCCCCCCHHHHH
LQWLQSQVGESAASLLDAAQGAPLRALALEQAEWFADRTRLLDTLVRVAEGRQSVAQASP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLAKHDPTEMASVIYGWLSRSLNQRFSGQQSSDALLAPLLERLNKAVPPARLLRAAQRVL
CHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
EGRRALLSGANPNKELLYEQWLLVLVGVDAAAAAH
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]