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The map label for this gene is rlmL

Identifier: 110833866

GI number: 110833866

Start: 1142748

End: 1144862

Strand: Direct

Name: rlmL

Synonym: ABO_1005

Alternate gene names: 110833866

Gene position: 1142748-1144862 (Clockwise)

Preceding gene: 110833864

Following gene: 110833867

Centisome position: 36.62

GC content: 55.32

Gene sequence:

>2115_bases
ATGGAATTTGTGGCCACCTGCATGCCAGGTCTGGCAGATCTGCTCAGCGACGAACTAGGCTCACTGGGCATATCCGTAAC
AGAAACCGGGCGTGCGCATGTCAGTTTCTCCGGGGGCGTGGCGGATGCTCTGAAGGTGTGCCTCTGGTCCCGTTTGTCCG
AGCGGGTGCTGCTGCATCTGGCCACGTTGGGTGTAACACCGGATATTGCACCGGAAAAGTTGGCGGCTAGCCAGGATTGG
CTATCTTTGGTGGGGAACAATGCACCGGTACATCTGCATATTGAGCACGGCGCTGAAGTGCGTGGTGATAATCGGATCAG
TGCCAAACGGTTTATTCATGCATTACCGGAGCAGTTTGCCATCTCCCGTGAGCCGAAAGGCAGTTGCTGTATTCGTGCCC
GGCTGGATTTGAATGAGGCACATCTGTGGTTGGATCTGGCCGGCGAGCCTTTGCATCGCCGTGGATATCGCCTCGCAGGC
GGTCGTGCGCCGCTACGCGAAACCTTGGCTGCAGCCATATTATGGGCTGTAGGGTGGGGCAAGGAAGGTCGTCATTCCGC
GCTGATCGATCCGTTTTGCGGTAGTGGTACCCTGGTGATTGAAGCGGCGCTGATAGCAGCAGGCCGCGCCGCAGGTGCCC
AGCGTCAGCACTATGGCTTTCAGCAGTGGCGTGGTTGTCGGCGCCAATTGTGGTTGGATGCCCAGAAGGAAGCAGGTGAG
AGTGGTAAGCGGCCAGTGCCAGCCGTATCGCTGAAAGGGTTTGATTCTGAAGCGAGCACATTGCAATTGGCTTTGCAGAA
CGCAGAGCGCGCCGGAGTGCGTAACGCAATTCATTTTGAGCGCCGGGAACTGGGTGCTCTGCGGCGTCGAGATTTTGTTG
ATAACGGTATTGTGGTGGGTAACCCGCCTTGGGGTGAGCGCCTGGAGGAAAAACCACAAGCGGGCTGGCTGCATTATGCG
CTGGGTCGTATTTTGTCGGTTCGAGCGCCGGGCTACCAGGCATTGTTGCTGGGTGCGGATGCGGAAGTCATGGACCGCTC
CGGGATGAATTTGGAACAGCAATGGCGCCTGAAAAATGGCCCGTTCAATAACTTTATCCGTCTTTATTCGCCCCGTAAGC
AGACGCCAGCCAATGTGGTGAAAGTTGCTGATGATAGTGCGTTCAACGTGCCCGAGGGCGCGCAACCCTTATTAAATCGG
CTGCGTAAAAATGGCAAACACCTGCGCCGTTGGCTGGAGCGGGAGGACATTCAGTGCTACCGGCTGTATGACCGCGATTT
GCCAGAATTCAATGTGGCCGTGGATATCTATGCGGATCAGGTGTTGGTGCAGGAGTTCAAGGCACCTAAAACCATTGATC
CGGAAAAAGCGAAAGAGCGCCGTGATTGGGCGGTGACAGCGGTGCGAGCGGCCTTAGGTGTTCACCGTGAGCAGGTGCAC
CTGCGCACCCGTGAAAAGCAGAAGGGGAATCAGCAATATCAAAAGCTGGATGCTCAGGGACAATACCGGGTTGTGCGTGA
AGGGCAATCGCAGTTGTTGATTAATTTGCAGGATTACTTGGATACCGGGCTATTTCTCGATCACCGGCCCACGCGTTTGC
GTATCGCTGAAGAGGCCAGTGGTAAGCGCTTCCTTAACCTGTTTGCGTACACGGGGTCTGCCACGGTGCATGCTGCTGTG
GGTGGCGCAAAGCGCACCGTTACGGTGGATTCCTCCAAGCGGTATCTGGATTGGGCAGCCTGCAACCTGGCCGCTAACGG
TTTCTCCACCGATCAGCATGAATTGGCTCGGGCCGACACCATGCGTTGGCTGGATGAGTGTAAGGAGCAGTTTGATCTGG
TGTTCTGCGATCCGCCCACTTTTTCCAATAATAAGTCACGGAGTGACTTTGTGGTGGAAGAGCATCACGGTGATTTGATT
CGCAAGATTATGCGCTGCTTGGAACCGGGTGGGGTGCTGTATTTCTCCTGTAATTACCGTCGCTTCCAAATGGATGAAAG
CATCAGCAAGTGGTACGCGGTAGAGGATATCTCACGTTGGAGTATCCCGGAGGATTTCCGCCGCAATGAAAAAATCCATT
ATTGCTATGCCATTCGTCATGTGGAGGATGCATGA

Upstream 100 bases:

>100_bases
CGTATAAAGGACAGTTTGATGACAGTTTGTGTAATACATTGAAAAATAATGGTTTATTTTTTGTTTTGCACATAAATTAG
TCATTTTAGAGGATTAGATA

Downstream 100 bases:

>100_bases
CGGTGCTGTTGTATACCACCTTGGGGTGCCACCTTTGTGATCAGGCCAGGGAGCTGGTAGCCATGGTCAGGCCGGATCTT
GAAATTACGCTGGTAGATAT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MEFVATCMPGLADLLSDELGSLGISVTETGRAHVSFSGGVADALKVCLWSRLSERVLLHLATLGVTPDIAPEKLAASQDW
LSLVGNNAPVHLHIEHGAEVRGDNRISAKRFIHALPEQFAISREPKGSCCIRARLDLNEAHLWLDLAGEPLHRRGYRLAG
GRAPLRETLAAAILWAVGWGKEGRHSALIDPFCGSGTLVIEAALIAAGRAAGAQRQHYGFQQWRGCRRQLWLDAQKEAGE
SGKRPVPAVSLKGFDSEASTLQLALQNAERAGVRNAIHFERRELGALRRRDFVDNGIVVGNPPWGERLEEKPQAGWLHYA
LGRILSVRAPGYQALLLGADAEVMDRSGMNLEQQWRLKNGPFNNFIRLYSPRKQTPANVVKVADDSAFNVPEGAQPLLNR
LRKNGKHLRRWLEREDIQCYRLYDRDLPEFNVAVDIYADQVLVQEFKAPKTIDPEKAKERRDWAVTAVRAALGVHREQVH
LRTREKQKGNQQYQKLDAQGQYRVVREGQSQLLINLQDYLDTGLFLDHRPTRLRIAEEASGKRFLNLFAYTGSATVHAAV
GGAKRTVTVDSSKRYLDWAACNLAANGFSTDQHELARADTMRWLDECKEQFDLVFCDPPTFSNNKSRSDFVVEEHHGDLI
RKIMRCLEPGGVLYFSCNYRRFQMDESISKWYAVEDISRWSIPEDFRRNEKIHYCYAIRHVEDA

Sequences:

>Translated_704_residues
MEFVATCMPGLADLLSDELGSLGISVTETGRAHVSFSGGVADALKVCLWSRLSERVLLHLATLGVTPDIAPEKLAASQDW
LSLVGNNAPVHLHIEHGAEVRGDNRISAKRFIHALPEQFAISREPKGSCCIRARLDLNEAHLWLDLAGEPLHRRGYRLAG
GRAPLRETLAAAILWAVGWGKEGRHSALIDPFCGSGTLVIEAALIAAGRAAGAQRQHYGFQQWRGCRRQLWLDAQKEAGE
SGKRPVPAVSLKGFDSEASTLQLALQNAERAGVRNAIHFERRELGALRRRDFVDNGIVVGNPPWGERLEEKPQAGWLHYA
LGRILSVRAPGYQALLLGADAEVMDRSGMNLEQQWRLKNGPFNNFIRLYSPRKQTPANVVKVADDSAFNVPEGAQPLLNR
LRKNGKHLRRWLEREDIQCYRLYDRDLPEFNVAVDIYADQVLVQEFKAPKTIDPEKAKERRDWAVTAVRAALGVHREQVH
LRTREKQKGNQQYQKLDAQGQYRVVREGQSQLLINLQDYLDTGLFLDHRPTRLRIAEEASGKRFLNLFAYTGSATVHAAV
GGAKRTVTVDSSKRYLDWAACNLAANGFSTDQHELARADTMRWLDECKEQFDLVFCDPPTFSNNKSRSDFVVEEHHGDLI
RKIMRCLEPGGVLYFSCNYRRFQMDESISKWYAVEDISRWSIPEDFRRNEKIHYCYAIRHVEDA
>Mature_704_residues
MEFVATCMPGLADLLSDELGSLGISVTETGRAHVSFSGGVADALKVCLWSRLSERVLLHLATLGVTPDIAPEKLAASQDW
LSLVGNNAPVHLHIEHGAEVRGDNRISAKRFIHALPEQFAISREPKGSCCIRARLDLNEAHLWLDLAGEPLHRRGYRLAG
GRAPLRETLAAAILWAVGWGKEGRHSALIDPFCGSGTLVIEAALIAAGRAAGAQRQHYGFQQWRGCRRQLWLDAQKEAGE
SGKRPVPAVSLKGFDSEASTLQLALQNAERAGVRNAIHFERRELGALRRRDFVDNGIVVGNPPWGERLEEKPQAGWLHYA
LGRILSVRAPGYQALLLGADAEVMDRSGMNLEQQWRLKNGPFNNFIRLYSPRKQTPANVVKVADDSAFNVPEGAQPLLNR
LRKNGKHLRRWLEREDIQCYRLYDRDLPEFNVAVDIYADQVLVQEFKAPKTIDPEKAKERRDWAVTAVRAALGVHREQVH
LRTREKQKGNQQYQKLDAQGQYRVVREGQSQLLINLQDYLDTGLFLDHRPTRLRIAEEASGKRFLNLFAYTGSATVHAAV
GGAKRTVTVDSSKRYLDWAACNLAANGFSTDQHELARADTMRWLDECKEQFDLVFCDPPTFSNNKSRSDFVVEEHHGDLI
RKIMRCLEPGGVLYFSCNYRRFQMDESISKWYAVEDISRWSIPEDFRRNEKIHYCYAIRHVEDA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmL family

Homologues:

Organism=Escherichia coli, GI1787181, Length=693, Percent_Identity=38.961038961039, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI145693111, Length=302, Percent_Identity=26.8211920529801, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_ALCBS (Q0VQU5)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692725.1
- ProteinModelPortal:   Q0VQU5
- SMR:   Q0VQU5
- STRING:   Q0VQU5
- GeneID:   4212860
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1005
- NMPDR:   fig|393595.12.peg.1012
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   Q0VQU5
- ProtClustDB:   PRK11783
- BioCyc:   ABOR393595:ABO_1005-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- PIRSF:   PIRSF037618

Pfam domain/function: PF10672 Methyltrans_SAM; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79405; Mature: 79405

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFVATCMPGLADLLSDELGSLGISVTETGRAHVSFSGGVADALKVCLWSRLSERVLLHL
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ATLGVTPDIAPEKLAASQDWLSLVGNNAPVHLHIEHGAEVRGDNRISAKRFIHALPEQFA
HHHCCCCCCCHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHH
ISREPKGSCCIRARLDLNEAHLWLDLAGEPLHRRGYRLAGGRAPLRETLAAAILWAVGWG
CCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHCCCEECCCCCHHHHHHHHHHHHHHCCC
KEGRHSALIDPFCGSGTLVIEAALIAAGRAAGAQRQHYGFQQWRGCRRQLWLDAQKEAGE
CCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHCCC
SGKRPVPAVSLKGFDSEASTLQLALQNAERAGVRNAIHFERRELGALRRRDFVDNGIVVG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
NPPWGERLEEKPQAGWLHYALGRILSVRAPGYQALLLGADAEVMDRSGMNLEQQWRLKNG
CCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCEEEEEECCHHHHHCCCCCHHHHHCCCCC
PFNNFIRLYSPRKQTPANVVKVADDSAFNVPEGAQPLLNRLRKNGKHLRRWLEREDIQCY
CHHCEEEECCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCEEE
RLYDRDLPEFNVAVDIYADQVLVQEFKAPKTIDPEKAKERRDWAVTAVRAALGVHREQVH
EECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LRTREKQKGNQQYQKLDAQGQYRVVREGQSQLLINLQDYLDTGLFLDHRPTRLRIAEEAS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHCCCCEECCCCCEEEEECCCC
GKRFLNLFAYTGSATVHAAVGGAKRTVTVDSSKRYLDWAACNLAANGFSTDQHELARADT
CCEEEEEEEECCCCEEEEEECCCEEEEEECCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
MRWLDECKEQFDLVFCDPPTFSNNKSRSDFVVEEHHGDLIRKIMRCLEPGGVLYFSCNYR
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEHHCCHHHHHHHHHCCCCCEEEEEECCE
RFQMDESISKWYAVEDISRWSIPEDFRRNEKIHYCYAIRHVEDA
EEECCHHHHHHEEHHHHHHCCCCHHHHCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MEFVATCMPGLADLLSDELGSLGISVTETGRAHVSFSGGVADALKVCLWSRLSERVLLHL
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ATLGVTPDIAPEKLAASQDWLSLVGNNAPVHLHIEHGAEVRGDNRISAKRFIHALPEQFA
HHHCCCCCCCHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHH
ISREPKGSCCIRARLDLNEAHLWLDLAGEPLHRRGYRLAGGRAPLRETLAAAILWAVGWG
CCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHCCCEECCCCCHHHHHHHHHHHHHHCCC
KEGRHSALIDPFCGSGTLVIEAALIAAGRAAGAQRQHYGFQQWRGCRRQLWLDAQKEAGE
CCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHCCC
SGKRPVPAVSLKGFDSEASTLQLALQNAERAGVRNAIHFERRELGALRRRDFVDNGIVVG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
NPPWGERLEEKPQAGWLHYALGRILSVRAPGYQALLLGADAEVMDRSGMNLEQQWRLKNG
CCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCEEEEEECCHHHHHCCCCCHHHHHCCCCC
PFNNFIRLYSPRKQTPANVVKVADDSAFNVPEGAQPLLNRLRKNGKHLRRWLEREDIQCY
CHHCEEEECCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCEEE
RLYDRDLPEFNVAVDIYADQVLVQEFKAPKTIDPEKAKERRDWAVTAVRAALGVHREQVH
EECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LRTREKQKGNQQYQKLDAQGQYRVVREGQSQLLINLQDYLDTGLFLDHRPTRLRIAEEAS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHCCCCEECCCCCEEEEECCCC
GKRFLNLFAYTGSATVHAAVGGAKRTVTVDSSKRYLDWAACNLAANGFSTDQHELARADT
CCEEEEEEEECCCCEEEEEECCCEEEEEECCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
MRWLDECKEQFDLVFCDPPTFSNNKSRSDFVVEEHHGDLIRKIMRCLEPGGVLYFSCNYR
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEHHCCHHHHHHHHHCCCCCEEEEEECCE
RFQMDESISKWYAVEDISRWSIPEDFRRNEKIHYCYAIRHVEDA
EEECCHHHHHHEEHHHHHHCCCCHHHHCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA