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The map label for this gene is queF

Identifier: 110833741

GI number: 110833741

Start: 975449

End: 976273

Strand: Direct

Name: queF

Synonym: ABO_0880

Alternate gene names: 110833741

Gene position: 975449-976273 (Clockwise)

Preceding gene: 110833740

Following gene: 110833746

Centisome position: 31.26

GC content: 52.24

Gene sequence:

>825_bases
ATGAGTTATCTTAAAGAAACGCCGCTGGGTCGCTCCAGTGAGTATGTTGATCAATACATGCCTTCGCTGTTGTGTCCGGT
GCCGCGCTGGGACGCGCGCGAGGGCCTGGAGCTGGAAAGTGCCACACTGCCTTTTCACGGCTCGGATATTTGGAATGCCT
ACGAATTGTCCTGGCTCAATGAAAAGGGCAAGCCCATTGTGGCCATGTGCGAACTGCGTGTTCCGTGCACAACCCCGAAC
ATTGTTGAGTCCAAGTCCTTAAAGCTGTATCTGAATTCGTTTGCCAATACTCGCTTTCAAAGCCGCGATGCGGTGCGTGC
TGCTATTGAGAAAGATGTGGCACAAACAGCAGGGGGCAACATTGAGGTATTACTGTTTTCGCTGCAGGAGTCGGCGGGTT
TCCCTGTGTGGGAAGATCGCGGCGATTGCGTGGACAATATAGATCTGAATTTCGAGCATTACGAGTACCGGCCTGATTTG
CTGTTATGCGATCAGGGCCCCGAACAAACTGGCCAGTTGTATTCTCATTTGTTGCGCAGCCATTGTCCGGTTACCGATCA
GCCAGACTGGGCGACAGTGGTCGTTCGTTACACCGGTCGTGCTATCAGCCCGGCCAGCTTTTTGCGCTATGTGGTATCGC
TGCGAAATCATCAGGGCTTTCACGAGCAGATTATTGAACAAATGTTTGTCGATCTGATGACGCAATGTTCGCCTCGCCAC
CTGACCGTTTACGGTCGTTTTACCCGGCGTGGTGGAATTGATATCAATCCGTTCCGCTCAAACAGTGAGCAGCCATTACC
GAACCGTCGCACTATTCGCCAGTAA

Upstream 100 bases:

>100_bases
TGCATCGTGGTACCGGTATCCGGCACTAACCAATGGGCTTGAATCCGGTTATCTTTAACCATTGCCGACGACCCATGGTG
GGGGCTACCAAGAGGTTATC

Downstream 100 bases:

>100_bases
GGGAAAGTAGCGGCGGGTTTGTGGTTTTGCTGTTTACCTTCGTGTCATTGGCGTTATCTTGTTGCCGCCTTCTGTCTAGT
ATGGCTGGAGGTACGGCTTC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSYLKETPLGRSSEYVDQYMPSLLCPVPRWDAREGLELESATLPFHGSDIWNAYELSWLNEKGKPIVAMCELRVPCTTPN
IVESKSLKLYLNSFANTRFQSRDAVRAAIEKDVAQTAGGNIEVLLFSLQESAGFPVWEDRGDCVDNIDLNFEHYEYRPDL
LLCDQGPEQTGQLYSHLLRSHCPVTDQPDWATVVVRYTGRAISPASFLRYVVSLRNHQGFHEQIIEQMFVDLMTQCSPRH
LTVYGRFTRRGGIDINPFRSNSEQPLPNRRTIRQ

Sequences:

>Translated_274_residues
MSYLKETPLGRSSEYVDQYMPSLLCPVPRWDAREGLELESATLPFHGSDIWNAYELSWLNEKGKPIVAMCELRVPCTTPN
IVESKSLKLYLNSFANTRFQSRDAVRAAIEKDVAQTAGGNIEVLLFSLQESAGFPVWEDRGDCVDNIDLNFEHYEYRPDL
LLCDQGPEQTGQLYSHLLRSHCPVTDQPDWATVVVRYTGRAISPASFLRYVVSLRNHQGFHEQIIEQMFVDLMTQCSPRH
LTVYGRFTRRGGIDINPFRSNSEQPLPNRRTIRQ
>Mature_273_residues
SYLKETPLGRSSEYVDQYMPSLLCPVPRWDAREGLELESATLPFHGSDIWNAYELSWLNEKGKPIVAMCELRVPCTTPNI
VESKSLKLYLNSFANTRFQSRDAVRAAIEKDVAQTAGGNIEVLLFSLQESAGFPVWEDRGDCVDNIDLNFEHYEYRPDLL
LCDQGPEQTGQLYSHLLRSHCPVTDQPDWATVVVRYTGRAISPASFLRYVVSLRNHQGFHEQIIEQMFVDLMTQCSPRHL
TVYGRFTRRGGIDINPFRSNSEQPLPNRRTIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=268, Percent_Identity=47.3880597014925, Blast_Score=253, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ALCBS (Q0VR70)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692600.1
- ProteinModelPortal:   Q0VR70
- SMR:   Q0VR70
- STRING:   Q0VR70
- GeneID:   4212696
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0880
- NMPDR:   fig|393595.12.peg.884
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   Q0VR70
- ProtClustDB:   PRK11792
- BioCyc:   ABOR393595:ABO_0880-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31403; Mature: 31272

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLKETPLGRSSEYVDQYMPSLLCPVPRWDAREGLELESATLPFHGSDIWNAYELSWLN
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
EKGKPIVAMCELRVPCTTPNIVESKSLKLYLNSFANTRFQSRDAVRAAIEKDVAQTAGGN
CCCCEEEEEEEEECCCCCCCCCCCCHHEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
IEVLLFSLQESAGFPVWEDRGDCVDNIDLNFEHYEYRPDLLLCDQGPEQTGQLYSHLLRS
EEEEEEEEHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHH
HCPVTDQPDWATVVVRYTGRAISPASFLRYVVSLRNHQGFHEQIIEQMFVDLMTQCSPRH
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
LTVYGRFTRRGGIDINPFRSNSEQPLPNRRTIRQ
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYLKETPLGRSSEYVDQYMPSLLCPVPRWDAREGLELESATLPFHGSDIWNAYELSWLN
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
EKGKPIVAMCELRVPCTTPNIVESKSLKLYLNSFANTRFQSRDAVRAAIEKDVAQTAGGN
CCCCEEEEEEEEECCCCCCCCCCCCHHEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
IEVLLFSLQESAGFPVWEDRGDCVDNIDLNFEHYEYRPDLLLCDQGPEQTGQLYSHLLRS
EEEEEEEEHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHH
HCPVTDQPDWATVVVRYTGRAISPASFLRYVVSLRNHQGFHEQIIEQMFVDLMTQCSPRH
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
LTVYGRFTRRGGIDINPFRSNSEQPLPNRRTIRQ
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA