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The map label for this gene is opuD [H]

Identifier: 110833669

GI number: 110833669

Start: 901465

End: 903033

Strand: Direct

Name: opuD [H]

Synonym: ABO_0808

Alternate gene names: 110833669

Gene position: 901465-903033 (Clockwise)

Preceding gene: 110833668

Following gene: 110833671

Centisome position: 28.89

GC content: 52.77

Gene sequence:

>1569_bases
ATGAAAATTAATTACGAGACCGACTATGACGTCGGCCAAGATAACGTGCAGGTCATGGGAATGGACATGCACAATCCTGT
CTTCTTTATCAGTGCATTACTGATTCTTACTTTTGTGATTGGCACATTAATTTTCCCTGAACAAGCTAACGCTTTGCTAA
ATGGCTCCAAAGGCTGGGTTATTGAGCACTTTGACTGGTTATTCATGGTGAGCGGGAATGTCTTCGTTTTATTTTGCGTG
ATGTTGGCGGTACTGCCGTTGGGGAAAATTCGCCTCGGTGGGCAAAATGCCAAGCCGGAATTCTCTACTCTGTCCTGGTT
TGCCATGTTGTTCGCAGCAGGGATGGGCATTGGCCTGATGTTCTGGAGCGTGGCGGAACCGGTAGCCTATCTTAATGGGC
AATGGTACGGCACGCCGTTGGCGGTGGAAGCCGGCTCAGAAGAGGCCCGGCATATGGCGATGGGCGCTACCATGTACCAT
TGGGGTCTGCACCCTTGGGCAATTTACGCTGTAGTGGCATTGTCGCTGGCCTTTTTCTCCTACAATAAAGGAATGCCGCT
GACTATCCGATCGGCTTTTTACCCCTTGCTGGGTGAGCGCTGCTGGGGCTGGCCGGGGCATATCATCGATATTCTTGCTG
TGCTGGCGACGATCTTTGGTCTGGCCACGTCACTGGGGCTGGGTGCTCAACAGGCTTCCGGTGGCTTGCACTTCCTGTTT
GACACGCCCAACGCCATTAACGTACAGATCGGCATCATTGTCGGTGTTACCGCTGTTGCGGTGCTATCGGTGATCCGTGG
CTTGGATGGTGGTGTGAAAGTGCTCAGTAATATCAACATGAGCCTGGCCGTTTTCCTTCTGCTATTTGTGATTATCGCGG
GTCCCACCGCGTTGATTCTCACGGGTTTTGGCACCACGGCGGTGGATTACATCAGTAATATTATTCCTCTGAGTACTTGG
TTTGGCCGTGAAGACGGGGAATGGATGCACGGCTGGACCGTCTTCTACTGGGCGTGGTGGATTTCCTGGTCGCCGTTTGT
GGGGATGTTTATTGCGCGTATTTCCAAAGGCCGTACCGTGCGAGAATTCATTGTGGCAGTGTTGCTGGTTCCCACAGTGA
TCACCATTATCTGGATGACCGCGTTCGGTGCCACTGGGCTGGAGCAATCCATCAATGGCGTGGGTGAATTGGCAAATGGG
GTGGGTGATGCCTCCCTGGCCATGTTCCAGATGCTGGCTCACTTGCCGTGGACGGGGATTATTTCCTTCTTGGGTATTGC
GCTGGTGCTAGTGTTCTTCGTGACTTCGTCTGATTCGGGGTCTCTGGTGATTGATTCAATTACCGCTGGCGGCAAGCTCG
ATGCCCCGGTTCCTCAGCGTATTTTCTGGGCTGTAATGGAAGGGGTGATCGCTGGCGTGTTGCTGTACGGTGGCGGTAAA
GAAGCGCTCAAGGCTCTGCAATCCGGTGCGGTCACTACGGGCCTGCCGTTTACCATTGTATTGTTGTTTATGTGTGTGAG
CCTGTTTATGGCCTTATCGGCAGAACGTAAGCGCCTGAAACTGGCTTGA

Upstream 100 bases:

>100_bases
GCTATAGAGGAGATCAACTCGGTTTCCTCGCATCGTCCGAGCCGACTGGCTGCAAGGCAGGTAATCTGCCCCTCGAAGCA
AGACGGAATACGGAGCTTAG

Downstream 100 bases:

>100_bases
TCTACATTCTGTGATTGATAAAAATGCCCGCGTTATGCGGGCATTTTTATGTCGCTTAGCGGCTGCAACCTGGTATCAAC
CGGCCGTTTTTTCAAACGGC

Product: choline/carnitine/betaine transporter family protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKINYETDYDVGQDNVQVMGMDMHNPVFFISALLILTFVIGTLIFPEQANALLNGSKGWVIEHFDWLFMVSGNVFVLFCV
MLAVLPLGKIRLGGQNAKPEFSTLSWFAMLFAAGMGIGLMFWSVAEPVAYLNGQWYGTPLAVEAGSEEARHMAMGATMYH
WGLHPWAIYAVVALSLAFFSYNKGMPLTIRSAFYPLLGERCWGWPGHIIDILAVLATIFGLATSLGLGAQQASGGLHFLF
DTPNAINVQIGIIVGVTAVAVLSVIRGLDGGVKVLSNINMSLAVFLLLFVIIAGPTALILTGFGTTAVDYISNIIPLSTW
FGREDGEWMHGWTVFYWAWWISWSPFVGMFIARISKGRTVREFIVAVLLVPTVITIIWMTAFGATGLEQSINGVGELANG
VGDASLAMFQMLAHLPWTGIISFLGIALVLVFFVTSSDSGSLVIDSITAGGKLDAPVPQRIFWAVMEGVIAGVLLYGGGK
EALKALQSGAVTTGLPFTIVLLFMCVSLFMALSAERKRLKLA

Sequences:

>Translated_522_residues
MKINYETDYDVGQDNVQVMGMDMHNPVFFISALLILTFVIGTLIFPEQANALLNGSKGWVIEHFDWLFMVSGNVFVLFCV
MLAVLPLGKIRLGGQNAKPEFSTLSWFAMLFAAGMGIGLMFWSVAEPVAYLNGQWYGTPLAVEAGSEEARHMAMGATMYH
WGLHPWAIYAVVALSLAFFSYNKGMPLTIRSAFYPLLGERCWGWPGHIIDILAVLATIFGLATSLGLGAQQASGGLHFLF
DTPNAINVQIGIIVGVTAVAVLSVIRGLDGGVKVLSNINMSLAVFLLLFVIIAGPTALILTGFGTTAVDYISNIIPLSTW
FGREDGEWMHGWTVFYWAWWISWSPFVGMFIARISKGRTVREFIVAVLLVPTVITIIWMTAFGATGLEQSINGVGELANG
VGDASLAMFQMLAHLPWTGIISFLGIALVLVFFVTSSDSGSLVIDSITAGGKLDAPVPQRIFWAVMEGVIAGVLLYGGGK
EALKALQSGAVTTGLPFTIVLLFMCVSLFMALSAERKRLKLA
>Mature_522_residues
MKINYETDYDVGQDNVQVMGMDMHNPVFFISALLILTFVIGTLIFPEQANALLNGSKGWVIEHFDWLFMVSGNVFVLFCV
MLAVLPLGKIRLGGQNAKPEFSTLSWFAMLFAAGMGIGLMFWSVAEPVAYLNGQWYGTPLAVEAGSEEARHMAMGATMYH
WGLHPWAIYAVVALSLAFFSYNKGMPLTIRSAFYPLLGERCWGWPGHIIDILAVLATIFGLATSLGLGAQQASGGLHFLF
DTPNAINVQIGIIVGVTAVAVLSVIRGLDGGVKVLSNINMSLAVFLLLFVIIAGPTALILTGFGTTAVDYISNIIPLSTW
FGREDGEWMHGWTVFYWAWWISWSPFVGMFIARISKGRTVREFIVAVLLVPTVITIIWMTAFGATGLEQSINGVGELANG
VGDASLAMFQMLAHLPWTGIISFLGIALVLVFFVTSSDSGSLVIDSITAGGKLDAPVPQRIFWAVMEGVIAGVLLYGGGK
EALKALQSGAVTTGLPFTIVLLFMCVSLFMALSAERKRLKLA

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=509, Percent_Identity=39.4891944990177, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786224, Length=473, Percent_Identity=30.0211416490486, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=30.1369863013699, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56478; Mature: 56478

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINYETDYDVGQDNVQVMGMDMHNPVFFISALLILTFVIGTLIFPEQANALLNGSKGWV
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCE
IEHFDWLFMVSGNVFVLFCVMLAVLPLGKIRLGGQNAKPEFSTLSWFAMLFAAGMGIGLM
EECCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FWSVAEPVAYLNGQWYGTPLAVEAGSEEARHMAMGATMYHWGLHPWAIYAVVALSLAFFS
HHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHH
YNKGMPLTIRSAFYPLLGERCWGWPGHIIDILAVLATIFGLATSLGLGAQQASGGLHFLF
CCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEE
DTPNAINVQIGIIVGVTAVAVLSVIRGLDGGVKVLSNINMSLAVFLLLFVIIAGPTALIL
ECCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
TGFGTTAVDYISNIIPLSTWFGREDGEWMHGWTVFYWAWWISWSPFVGMFIARISKGRTV
ECCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHH
REFIVAVLLVPTVITIIWMTAFGATGLEQSINGVGELANGVGDASLAMFQMLAHLPWTGI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
ISFLGIALVLVFFVTSSDSGSLVIDSITAGGKLDAPVPQRIFWAVMEGVIAGVLLYGGGK
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
EALKALQSGAVTTGLPFTIVLLFMCVSLFMALSAERKRLKLA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKINYETDYDVGQDNVQVMGMDMHNPVFFISALLILTFVIGTLIFPEQANALLNGSKGWV
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCE
IEHFDWLFMVSGNVFVLFCVMLAVLPLGKIRLGGQNAKPEFSTLSWFAMLFAAGMGIGLM
EECCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FWSVAEPVAYLNGQWYGTPLAVEAGSEEARHMAMGATMYHWGLHPWAIYAVVALSLAFFS
HHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHH
YNKGMPLTIRSAFYPLLGERCWGWPGHIIDILAVLATIFGLATSLGLGAQQASGGLHFLF
CCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEE
DTPNAINVQIGIIVGVTAVAVLSVIRGLDGGVKVLSNINMSLAVFLLLFVIIAGPTALIL
ECCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
TGFGTTAVDYISNIIPLSTWFGREDGEWMHGWTVFYWAWWISWSPFVGMFIARISKGRTV
ECCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHH
REFIVAVLLVPTVITIIWMTAFGATGLEQSINGVGELANGVGDASLAMFQMLAHLPWTGI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
ISFLGIALVLVFFVTSSDSGSLVIDSITAGGKLDAPVPQRIFWAVMEGVIAGVLLYGGGK
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
EALKALQSGAVTTGLPFTIVLLFMCVSLFMALSAERKRLKLA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]