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The map label for this gene is aceF [H]

Identifier: 110833484

GI number: 110833484

Start: 687434

End: 689128

Strand: Direct

Name: aceF [H]

Synonym: ABO_0623

Alternate gene names: 110833484

Gene position: 687434-689128 (Clockwise)

Preceding gene: 110833483

Following gene: 110833494

Centisome position: 22.03

GC content: 53.51

Gene sequence:

>1695_bases
GTGAGTAAAACGCAAATATATGTTCCCGATGTGGGCAGCAGTGATCCTGTTGATATCATCGAGATCAGGGTTAACGTCGG
CGATACCATTAGCGCTGAAGACACGATTATCGTGCTTGAATCTGATAAGGCCACGGTGGAAGTGCCGGCGCCCCAGGGCG
GGAAGGTGGCTTCTATTAGTGTAAAGGTGGGTGACAGGGTTAAGGAGGGCGATGCAGTAATGGAGCTGGAGGTCGCCGAC
GGTGACGCTACCGATAGCGCCGAAGATGTATCGGACTCTGAGTCAGAAAACGAGACCGCCAAGGGCGGTAAAACCAACGA
TGCTAGTTCGCAGACGCAGAATGAAAATGCATCGGAGAAGCAGACAAAGTCCACTTCAGACAATAATGCGGATAAGGCTG
CCGAGCCGGCTGCAAGTAGATCGGAAACCGTAAAGGTGCCGGATCTGGGCGACATTGATGCCGCAGAGATCATTGAAGTG
AATGTGGCCGTGGGGGATGAGCTTGATGAAGAGCAAATCATCGTAGTGGTGGAATCAGACAAGGCGTCTTTGGAAATTCC
CTCACCAAAAGCCGGCAAGGTGGAATCCGTTAACGTTAGTGTCGGCGATAAGGTTGGTAGTGGTGATGCGTTGATTACCC
TTGCCGTTACCGGTACGCCGGCAGCAGAAGAGAGTGCACAGCCTGAGCGCGAACAGGATGGGGCAGAGCAGGATTCCTCT
CCTGAAACGGCTTCTTCTAGCGGTTCTACACCGTCCCGTGCGCAACCCTCTGATGCAGGTGGCTCGCCTTCCCGCCCCGT
TCATGCCGGCCCGGCGGTACGCAAATTGGCCCGGGAAACGGGGGTGGATTTGTCCCAGGTCAGCGGCACTGGCCCGAAAG
ATCGCATCCTCAAAGATGACGTGCATGCCTATGTGAAGCAGCGTTTGGAGGGGCCACAGAGCGGGGGCTCAGCGGGTGGA
ATGGCGATAGACTTGCCAGAAATCGATTTCAGCCAGTTTGGTGAAATTGAACGCGTTGAGTTGAACAAGCTGCGCAAGGT
GTCGGCGCAGAATCTGACCCGTTCATGGTTGACTATTCCCCATGTAACCCAGCATGACAATGCGGATATCACCGACTTGG
AGGCGTTTCGTAAGAGCCAGAACAAGCGTCTTGAACGTGAAGGGGTAAAGCTCACCATGTTGGCTTTCCTAGTAGCGGCC
TGTGCACGGGCGTTAAAGGAATATCCACGGTTTAACAGCTCTTTGGAAAATAGCGGCGAAGCATTGATTGAAAAGCGGTA
TATCAATATTGGTATCGCAGTCGATACACCTAATGGATTGGTTGTGCCGGTGATCAAGGATGCGGATAAAAAAGGGCTAA
AGGCGATTGCTCAGGAAATGGACGAACTGGCAGAGAAAGCCCGTAATCGGAAGCTGACCCCTGCGGACATGAAGGGCGGG
ACTTTCAGTATTTCGTCTTTGGGGGGGATTGGCGGTACGGCGTTCACCCCGATTGTAAACTGGCCGGAAGTGGCGATTCT
CGGCGTCAGCCGCTCCGATATGCAGCCGGTCTGGGATGGGAGTGAGTTCCAGCCGCGGTTAATACTGCCAATGAGCCTGT
CTTACGATCATCGAGTCATTGATGGTGCAGCAGCCGCACGATTTACGACTTATTTGAGCCAGTTATTAACGGATATGCGC
CAGGCATTGTTGTAA

Upstream 100 bases:

>100_bases
GAGAAATACGGCATTGACACGGATAAACCCTATCCGGTGATGCATTAATCGCCCCCGTCACTGATTACCGATCAGTGATC
TCACCATAAGGAGTTAAGGT

Downstream 100 bases:

>100_bases
TTGAGCTGGCAAACGTAGCCAAGAGGAATAAAAAAAGGCCTTCGTGTGAAGGCCTTTTTTAAAGTGCACTAAAAGCAAAG
GGTTAACGTTTTTCCTTTTT

Product: pyruvate dehydrogenase, E2 component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MSKTQIYVPDVGSSDPVDIIEIRVNVGDTISAEDTIIVLESDKATVEVPAPQGGKVASISVKVGDRVKEGDAVMELEVAD
GDATDSAEDVSDSESENETAKGGKTNDASSQTQNENASEKQTKSTSDNNADKAAEPAASRSETVKVPDLGDIDAAEIIEV
NVAVGDELDEEQIIVVVESDKASLEIPSPKAGKVESVNVSVGDKVGSGDALITLAVTGTPAAEESAQPEREQDGAEQDSS
PETASSSGSTPSRAQPSDAGGSPSRPVHAGPAVRKLARETGVDLSQVSGTGPKDRILKDDVHAYVKQRLEGPQSGGSAGG
MAIDLPEIDFSQFGEIERVELNKLRKVSAQNLTRSWLTIPHVTQHDNADITDLEAFRKSQNKRLEREGVKLTMLAFLVAA
CARALKEYPRFNSSLENSGEALIEKRYINIGIAVDTPNGLVVPVIKDADKKGLKAIAQEMDELAEKARNRKLTPADMKGG
TFSISSLGGIGGTAFTPIVNWPEVAILGVSRSDMQPVWDGSEFQPRLILPMSLSYDHRVIDGAAAARFTTYLSQLLTDMR
QALL

Sequences:

>Translated_564_residues
MSKTQIYVPDVGSSDPVDIIEIRVNVGDTISAEDTIIVLESDKATVEVPAPQGGKVASISVKVGDRVKEGDAVMELEVAD
GDATDSAEDVSDSESENETAKGGKTNDASSQTQNENASEKQTKSTSDNNADKAAEPAASRSETVKVPDLGDIDAAEIIEV
NVAVGDELDEEQIIVVVESDKASLEIPSPKAGKVESVNVSVGDKVGSGDALITLAVTGTPAAEESAQPEREQDGAEQDSS
PETASSSGSTPSRAQPSDAGGSPSRPVHAGPAVRKLARETGVDLSQVSGTGPKDRILKDDVHAYVKQRLEGPQSGGSAGG
MAIDLPEIDFSQFGEIERVELNKLRKVSAQNLTRSWLTIPHVTQHDNADITDLEAFRKSQNKRLEREGVKLTMLAFLVAA
CARALKEYPRFNSSLENSGEALIEKRYINIGIAVDTPNGLVVPVIKDADKKGLKAIAQEMDELAEKARNRKLTPADMKGG
TFSISSLGGIGGTAFTPIVNWPEVAILGVSRSDMQPVWDGSEFQPRLILPMSLSYDHRVIDGAAAARFTTYLSQLLTDMR
QALL
>Mature_563_residues
SKTQIYVPDVGSSDPVDIIEIRVNVGDTISAEDTIIVLESDKATVEVPAPQGGKVASISVKVGDRVKEGDAVMELEVADG
DATDSAEDVSDSESENETAKGGKTNDASSQTQNENASEKQTKSTSDNNADKAAEPAASRSETVKVPDLGDIDAAEIIEVN
VAVGDELDEEQIIVVVESDKASLEIPSPKAGKVESVNVSVGDKVGSGDALITLAVTGTPAAEESAQPEREQDGAEQDSSP
ETASSSGSTPSRAQPSDAGGSPSRPVHAGPAVRKLARETGVDLSQVSGTGPKDRILKDDVHAYVKQRLEGPQSGGSAGGM
AIDLPEIDFSQFGEIERVELNKLRKVSAQNLTRSWLTIPHVTQHDNADITDLEAFRKSQNKRLEREGVKLTMLAFLVAAC
ARALKEYPRFNSSLENSGEALIEKRYINIGIAVDTPNGLVVPVIKDADKKGLKAIAQEMDELAEKARNRKLTPADMKGGT
FSISSLGGIGGTAFTPIVNWPEVAILGVSRSDMQPVWDGSEFQPRLILPMSLSYDHRVIDGAAAARFTTYLSQLLTDMRQ
ALL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=560, Percent_Identity=28.0357142857143, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=28.7640449438202, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=35.6521739130435, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI203098816, Length=324, Percent_Identity=29.6296296296296, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI203098753, Length=309, Percent_Identity=30.0970873786408, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI260898739, Length=149, Percent_Identity=38.9261744966443, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786305, Length=566, Percent_Identity=42.9328621908127, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=29.925187032419, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=411, Percent_Identity=31.8734793187348, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=427, Percent_Identity=30.6791569086651, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=31.2056737588652, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=32.1311475409836, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=437, Percent_Identity=28.8329519450801, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=443, Percent_Identity=32.2799097065463, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=32.15859030837, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=32.15859030837, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24645909, Length=213, Percent_Identity=34.2723004694836, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 59826; Mature: 59695

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTQIYVPDVGSSDPVDIIEIRVNVGDTISAEDTIIVLESDKATVEVPAPQGGKVASIS
CCCCEEEECCCCCCCCCEEEEEEEECCCEECCCCEEEEEECCCCEEEECCCCCCEEEEEE
VKVGDRVKEGDAVMELEVADGDATDSAEDVSDSESENETAKGGKTNDASSQTQNENASEK
EEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QTKSTSDNNADKAAEPAASRSETVKVPDLGDIDAAEIIEVNVAVGDELDEEQIIVVVESD
HHCCCCCCCCCHHCCCCCCCCCEEECCCCCCCCHHHEEEEEEEECCCCCCCEEEEEEECC
KASLEIPSPKAGKVESVNVSVGDKVGSGDALITLAVTGTPAAEESAQPEREQDGAEQDSS
CCEEECCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCCC
PETASSSGSTPSRAQPSDAGGSPSRPVHAGPAVRKLARETGVDLSQVSGTGPKDRILKDD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHH
VHAYVKQRLEGPQSGGSAGGMAIDLPEIDFSQFGEIERVELNKLRKVSAQNLTRSWLTIP
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHEECC
HVTQHDNADITDLEAFRKSQNKRLEREGVKLTMLAFLVAACARALKEYPRFNSSLENSGE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCH
ALIEKRYINIGIAVDTPNGLVVPVIKDADKKGLKAIAQEMDELAEKARNRKLTPADMKGG
HHEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TFSISSLGGIGGTAFTPIVNWPEVAILGVSRSDMQPVWDGSEFQPRLILPMSLSYDHRVI
EEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEH
DGAAAARFTTYLSQLLTDMRQALL
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTQIYVPDVGSSDPVDIIEIRVNVGDTISAEDTIIVLESDKATVEVPAPQGGKVASIS
CCCEEEECCCCCCCCCEEEEEEEECCCEECCCCEEEEEECCCCEEEECCCCCCEEEEEE
VKVGDRVKEGDAVMELEVADGDATDSAEDVSDSESENETAKGGKTNDASSQTQNENASEK
EEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QTKSTSDNNADKAAEPAASRSETVKVPDLGDIDAAEIIEVNVAVGDELDEEQIIVVVESD
HHCCCCCCCCCHHCCCCCCCCCEEECCCCCCCCHHHEEEEEEEECCCCCCCEEEEEEECC
KASLEIPSPKAGKVESVNVSVGDKVGSGDALITLAVTGTPAAEESAQPEREQDGAEQDSS
CCEEECCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCCC
PETASSSGSTPSRAQPSDAGGSPSRPVHAGPAVRKLARETGVDLSQVSGTGPKDRILKDD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHH
VHAYVKQRLEGPQSGGSAGGMAIDLPEIDFSQFGEIERVELNKLRKVSAQNLTRSWLTIP
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHEECC
HVTQHDNADITDLEAFRKSQNKRLEREGVKLTMLAFLVAACARALKEYPRFNSSLENSGE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCH
ALIEKRYINIGIAVDTPNGLVVPVIKDADKKGLKAIAQEMDELAEKARNRKLTPADMKGG
HHEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TFSISSLGGIGGTAFTPIVNWPEVAILGVSRSDMQPVWDGSEFQPRLILPMSLSYDHRVI
EEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEH
DGAAAARFTTYLSQLLTDMRQALL
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171401; 10984043 [H]