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The map label for this gene is tgt [H]

Identifier: 110833362

GI number: 110833362

Start: 550288

End: 551409

Strand: Direct

Name: tgt [H]

Synonym: ABO_0501

Alternate gene names: 110833362

Gene position: 550288-551409 (Clockwise)

Preceding gene: 110833361

Following gene: 110833363

Centisome position: 17.64

GC content: 56.51

Gene sequence:

>1122_bases
ATGTCCCGAATGACGTTCAAGCTCAGCACCACCGACGGTGCTGCCCGCCGTGGTGAAATTACGTTTCCCCGCGGCACCAT
TCAAACGCCGGCCTTTATGCCGGTAGGCACCTATGGCACGGTGAAAAGCATGCTGCCTAGGGATCTTGCCGATACTGGCG
CCGAGATTTGTCTGGGAAACACCTTCCATTTGATGCTGCGCCCGGGCACTGAAGTCATTGAGGCTCACGGTTCCTTGCAT
GGCTTTATGCAGTGGGACAAACCGATCTTGACGGATTCCGGCGGCTTTCAGGTGTTCAGCCTTGGTGAGATGCGCAAGAT
TTCCGAGCAGGGCGTGGTGTTCAGAAACCCTATCAATGGTGACAAGGTTGAACTGAGCCCGGAAAAAGCCATGCAGGTAC
AGCGCTCGCTGGGCAGCGATATCTGCATGATCTTTGATGAATGCACTCCTTTCCCGGCCACTGAAAAACAGGCCCGTGAT
TCCATGGAGCTGTCCCTGCGCTGGGCGAAGCGCTCAAAAGACGCCCATGAAGGCAATGATGCCGCCTGTTTCGGTATCGT
GCAGGGTGGCATGTATGATGATCTGCGCCGCGAATCCCTGGCCGGGCTGGTGGATATTGGCTTTGACGGCTACGCGGTAG
GTGGCTTGAGTGTGGGCGAGCCCCAGAACGAGATGTTACGAACGCTGGGTAATCTGACCCCGCATATGCCGGAAGACCAC
CCCCGTTACTTGATGGGCGTGGGTAAGCCGGATGATATTGTTGAGGCGGTACGCCGGGGGGTGGACATGTTTGATTGCGT
GATGCCTACCCGCAATGCCCGCAATGGCCACCTGTTCATCGCCGAAGGGGTGGTGAAAATTCGGAATGCCCGGCACCGCC
ATGATCTGGCTCCTCTGGAGGCAGAGTGTGACTGTTATACCTGCCAGAATTTCTCCCGCAGCTATTTGCACCATCTGGAT
CGGTGCAATGAAATGCTGGGCTCCCAGCTCAATACTATCCATAACCTGCGGTATTACCAGCGGTTGATGGCTGGATTGCG
GGGGGCCATTGAAGGGGGTACATTGTCCGCCTTTGTGGACGACTTTTATGCAGCCCGAGGTCAACAAACCCCGGCGCTGT
AG

Upstream 100 bases:

>100_bases
CGCAATACCCAGCACGTGAAAAGCGGAGCTGACGAATGACCAAGATTTCCTTTTTTGGCGTGCTGCGTATTGCGTGGCGC
ATGCAAGTGAGCGCTTATCC

Downstream 100 bases:

>100_bases
CTATTTATTGATTGGAGTAATTAATGAACATCGCAATGCGTTCCCTGTTGGCCCTGATGGCTGCGGCCATGGTTTCTCCG
GCGTTTGCAGCTCCTGCTGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSRMTFKLSTTDGAARRGEITFPRGTIQTPAFMPVGTYGTVKSMLPRDLADTGAEICLGNTFHLMLRPGTEVIEAHGSLH
GFMQWDKPILTDSGGFQVFSLGEMRKISEQGVVFRNPINGDKVELSPEKAMQVQRSLGSDICMIFDECTPFPATEKQARD
SMELSLRWAKRSKDAHEGNDAACFGIVQGGMYDDLRRESLAGLVDIGFDGYAVGGLSVGEPQNEMLRTLGNLTPHMPEDH
PRYLMGVGKPDDIVEAVRRGVDMFDCVMPTRNARNGHLFIAEGVVKIRNARHRHDLAPLEAECDCYTCQNFSRSYLHHLD
RCNEMLGSQLNTIHNLRYYQRLMAGLRGAIEGGTLSAFVDDFYAARGQQTPAL

Sequences:

>Translated_373_residues
MSRMTFKLSTTDGAARRGEITFPRGTIQTPAFMPVGTYGTVKSMLPRDLADTGAEICLGNTFHLMLRPGTEVIEAHGSLH
GFMQWDKPILTDSGGFQVFSLGEMRKISEQGVVFRNPINGDKVELSPEKAMQVQRSLGSDICMIFDECTPFPATEKQARD
SMELSLRWAKRSKDAHEGNDAACFGIVQGGMYDDLRRESLAGLVDIGFDGYAVGGLSVGEPQNEMLRTLGNLTPHMPEDH
PRYLMGVGKPDDIVEAVRRGVDMFDCVMPTRNARNGHLFIAEGVVKIRNARHRHDLAPLEAECDCYTCQNFSRSYLHHLD
RCNEMLGSQLNTIHNLRYYQRLMAGLRGAIEGGTLSAFVDDFYAARGQQTPAL
>Mature_372_residues
SRMTFKLSTTDGAARRGEITFPRGTIQTPAFMPVGTYGTVKSMLPRDLADTGAEICLGNTFHLMLRPGTEVIEAHGSLHG
FMQWDKPILTDSGGFQVFSLGEMRKISEQGVVFRNPINGDKVELSPEKAMQVQRSLGSDICMIFDECTPFPATEKQARDS
MELSLRWAKRSKDAHEGNDAACFGIVQGGMYDDLRRESLAGLVDIGFDGYAVGGLSVGEPQNEMLRTLGNLTPHMPEDHP
RYLMGVGKPDDIVEAVRRGVDMFDCVMPTRNARNGHLFIAEGVVKIRNARHRHDLAPLEAECDCYTCQNFSRSYLHHLDR
CNEMLGSQLNTIHNLRYYQRLMAGLRGAIEGGTLSAFVDDFYAARGQQTPAL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=43.1818181818182, Blast_Score=301, Evalue=5e-82,
Organism=Homo sapiens, GI13375872, Length=287, Percent_Identity=24.7386759581882, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=68.010752688172, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17544680, Length=374, Percent_Identity=38.2352941176471, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=180, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=41.1290322580645, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI21355311, Length=261, Percent_Identity=23.7547892720307, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41372; Mature: 41241

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMTFKLSTTDGAARRGEITFPRGTIQTPAFMPVGTYGTVKSMLPRDLADTGAEICLGN
CCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECC
TFHLMLRPGTEVIEAHGSLHGFMQWDKPILTDSGGFQVFSLGEMRKISEQGVVFRNPING
EEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCC
DKVELSPEKAMQVQRSLGSDICMIFDECTPFPATEKQARDSMELSLRWAKRSKDAHEGND
CEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCHHHEEHHHHCCCCCCCCCC
AACFGIVQGGMYDDLRRESLAGLVDIGFDGYAVGGLSVGEPQNEMLRTLGNLTPHMPEDH
EEEEEEECCCCHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
PRYLMGVGKPDDIVEAVRRGVDMFDCVMPTRNARNGHLFIAEGVVKIRNARHRHDLAPLE
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCHHHHHCCHHHCCCCCCC
AECDCYTCQNFSRSYLHHLDRCNEMLGSQLNTIHNLRYYQRLMAGLRGAIEGGTLSAFVD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DFYAARGQQTPAL
HHHHHCCCCCCCC
>Mature Secondary Structure 
SRMTFKLSTTDGAARRGEITFPRGTIQTPAFMPVGTYGTVKSMLPRDLADTGAEICLGN
CCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECC
TFHLMLRPGTEVIEAHGSLHGFMQWDKPILTDSGGFQVFSLGEMRKISEQGVVFRNPING
EEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCC
DKVELSPEKAMQVQRSLGSDICMIFDECTPFPATEKQARDSMELSLRWAKRSKDAHEGND
CEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCHHHEEHHHHCCCCCCCCCC
AACFGIVQGGMYDDLRRESLAGLVDIGFDGYAVGGLSVGEPQNEMLRTLGNLTPHMPEDH
EEEEEEECCCCHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
PRYLMGVGKPDDIVEAVRRGVDMFDCVMPTRNARNGHLFIAEGVVKIRNARHRHDLAPLE
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCHHHHHCCHHHCCCCCCC
AECDCYTCQNFSRSYLHHLDRCNEMLGSQLNTIHNLRYYQRLMAGLRGAIEGGTLSAFVD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DFYAARGQQTPAL
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA