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The map label for this gene is queA

Identifier: 110833361

GI number: 110833361

Start: 549147

End: 550226

Strand: Direct

Name: queA

Synonym: ABO_0500

Alternate gene names: 110833361

Gene position: 549147-550226 (Clockwise)

Preceding gene: 110833360

Following gene: 110833362

Centisome position: 17.6

GC content: 56.48

Gene sequence:

>1080_bases
GTGAATGTCGACGATTTTGATTTTGAGCTCCCCGACAGGTTAATCGCCCGTCACCCGCCTGCGCAACGCCGTGATGCGCG
TTTGTTGGCGCTTACCTGCGATGCGCTTGAGCATCAGCAGTTTCCTGATTTGCTCTCCCATGTGCACCCCGGTGATCTAT
TGATCTTCAACGATACCCGCGTCATTCCTGCCCGGTTGTTTGGCCAGAAGGAAAGTGGCGGCAAGGTAGAAGTGTTGATA
GAGCGAGTGGTGGATGATCATGAAGCGCTTGCCCACGTGCGCGCCTCCAAGTCACCGAAACCCGGTAGCTGGTTGGAATT
TGCTAAAGGCATTCGTGCCCAGGTAACAGGGCGCCGGGGTGCTCTGTTTATCCTGCACTTCTCGTTGCCAGGAAACGGCT
GTGACACTCTTCTGAGCGCATTGGAACTGATTGGCCATGTGCCACTGCCTCCCTATATCGACCGCCCAGATGAGGACGGT
GATATGGAACGCTACCAAACGGTGTATGCTCGCGAGCCGGGGGCTGTTGCGGCTCCGACGGCCGGTCTGCATTTTGATGA
TGCGATGCTGGCGGCTTTGGAACAGCGCGGGGTTGATATCGGTTTTGTCACTCTTCATGTGGGCGCAGGGACGTTTCAGC
CGGTGCGTGTGGATAAAGTGGAAGACCACCATATGCACAGTGAGCGTTACCAGATTCCCGGTTCGCTAGTGGAGCAGGTG
GCCCAGGCTCATGCTCGGGGCGGTCGAGTGGTGGCGGTAGGCACTACTGCGCTGCGTGCGCTGGAAGCGGCCAGTCAGTC
AGGTGGTTTGCACGCTGGTCAGGGTGAAACGGATATCTTTATTTTTCCTGGTTACCGGTTCCGCTTGGTGGATGCTTTGG
TGACCAACTTTCATTTGCCTAAATCGACACTGCTGATGCTGATTAGTGCGTTCGCCGGCCGAGATCGGATCATGGGCGCC
TACCGGGCGGCCATTGAGGCCCAATACCGGTTTTTCAGTTATGGCGATGCCATGTTTATTGAGGGAACGCAGCCGGCATC
ACGCAATACCCAGCACGTGAAAAGCGGAGCTGACGAATGA

Upstream 100 bases:

>100_bases
TAGGTGAATTTTGCGCTTTCTGTAGTGGATACAGGTATTTTTTCCCGGCCGGATAGCGAGTATCCTTGCGGCCCCTTTAA
TTTCCGTGCCGGTACAGTAC

Downstream 100 bases:

>100_bases
CCAAGATTTCCTTTTTTGGCGTGCTGCGTATTGCGTGGCGCATGCAAGTGAGCGCTTATCCATGTCCCGAATGACGTTCA
AGCTCAGCACCACCGACGGT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNVDDFDFELPDRLIARHPPAQRRDARLLALTCDALEHQQFPDLLSHVHPGDLLIFNDTRVIPARLFGQKESGGKVEVLI
ERVVDDHEALAHVRASKSPKPGSWLEFAKGIRAQVTGRRGALFILHFSLPGNGCDTLLSALELIGHVPLPPYIDRPDEDG
DMERYQTVYAREPGAVAAPTAGLHFDDAMLAALEQRGVDIGFVTLHVGAGTFQPVRVDKVEDHHMHSERYQIPGSLVEQV
AQAHARGGRVVAVGTTALRALEAASQSGGLHAGQGETDIFIFPGYRFRLVDALVTNFHLPKSTLLMLISAFAGRDRIMGA
YRAAIEAQYRFFSYGDAMFIEGTQPASRNTQHVKSGADE

Sequences:

>Translated_359_residues
MNVDDFDFELPDRLIARHPPAQRRDARLLALTCDALEHQQFPDLLSHVHPGDLLIFNDTRVIPARLFGQKESGGKVEVLI
ERVVDDHEALAHVRASKSPKPGSWLEFAKGIRAQVTGRRGALFILHFSLPGNGCDTLLSALELIGHVPLPPYIDRPDEDG
DMERYQTVYAREPGAVAAPTAGLHFDDAMLAALEQRGVDIGFVTLHVGAGTFQPVRVDKVEDHHMHSERYQIPGSLVEQV
AQAHARGGRVVAVGTTALRALEAASQSGGLHAGQGETDIFIFPGYRFRLVDALVTNFHLPKSTLLMLISAFAGRDRIMGA
YRAAIEAQYRFFSYGDAMFIEGTQPASRNTQHVKSGADE
>Mature_359_residues
MNVDDFDFELPDRLIARHPPAQRRDARLLALTCDALEHQQFPDLLSHVHPGDLLIFNDTRVIPARLFGQKESGGKVEVLI
ERVVDDHEALAHVRASKSPKPGSWLEFAKGIRAQVTGRRGALFILHFSLPGNGCDTLLSALELIGHVPLPPYIDRPDEDG
DMERYQTVYAREPGAVAAPTAGLHFDDAMLAALEQRGVDIGFVTLHVGAGTFQPVRVDKVEDHHMHSERYQIPGSLVEQV
AQAHARGGRVVAVGTTALRALEAASQSGGLHAGQGETDIFIFPGYRFRLVDALVTNFHLPKSTLLMLISAFAGRDRIMGA
YRAAIEAQYRFFSYGDAMFIEGTQPASRNTQHVKSGADE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=59.0778097982709, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ALCBS (Q0VSA0)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692220.1
- ProteinModelPortal:   Q0VSA0
- SMR:   Q0VSA0
- STRING:   Q0VSA0
- GeneID:   4211646
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0500
- NMPDR:   fig|393595.12.peg.496
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q0VSA0
- BioCyc:   ABOR393595:ABO_0500-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39410; Mature: 39410

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDDFDFELPDRLIARHPPAQRRDARLLALTCDALEHQQFPDLLSHVHPGDLLIFNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCC
VIPARLFGQKESGGKVEVLIERVVDDHEALAHVRASKSPKPGSWLEFAKGIRAQVTGRRG
EEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCC
ALFILHFSLPGNGCDTLLSALELIGHVPLPPYIDRPDEDGDMERYQTVYAREPGAVAAPT
EEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
AGLHFDDAMLAALEQRGVDIGFVTLHVGAGTFQPVRVDKVEDHHMHSERYQIPGSLVEQV
CCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHCCCHHHCCCHHHHHHH
AQAHARGGRVVAVGTTALRALEAASQSGGLHAGQGETDIFIFPGYRFRLVDALVTNFHLP
HHHHHCCCEEEEECHHHHHHHHHHHHCCCEECCCCCCEEEEECCCHHHHHHHHHHHCCCC
KSTLLMLISAFAGRDRIMGAYRAAIEAQYRFFSYGDAMFIEGTQPASRNTQHVKSGADE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MNVDDFDFELPDRLIARHPPAQRRDARLLALTCDALEHQQFPDLLSHVHPGDLLIFNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCC
VIPARLFGQKESGGKVEVLIERVVDDHEALAHVRASKSPKPGSWLEFAKGIRAQVTGRRG
EEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCC
ALFILHFSLPGNGCDTLLSALELIGHVPLPPYIDRPDEDGDMERYQTVYAREPGAVAAPT
EEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
AGLHFDDAMLAALEQRGVDIGFVTLHVGAGTFQPVRVDKVEDHHMHSERYQIPGSLVEQV
CCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHCCCHHHCCCHHHHHHH
AQAHARGGRVVAVGTTALRALEAASQSGGLHAGQGETDIFIFPGYRFRLVDALVTNFHLP
HHHHHCCCEEEEECHHHHHHHHHHHHCCCEECCCCCCEEEEECCCHHHHHHHHHHHCCCC
KSTLLMLISAFAGRDRIMGAYRAAIEAQYRFFSYGDAMFIEGTQPASRNTQHVKSGADE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA