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The map label for this gene is proB

Identifier: 110833316

GI number: 110833316

Start: 494452

End: 495582

Strand: Direct

Name: proB

Synonym: ABO_0455

Alternate gene names: 110833316

Gene position: 494452-495582 (Clockwise)

Preceding gene: 110833315

Following gene: 110833318

Centisome position: 15.85

GC content: 59.59

Gene sequence:

>1131_bases
GTGGCGGATCGCCAGCGTTGGGTAATCAAGATAGGCAGTGCCTTGCTGACCAATGACGGCAAGGGCTTGGATTGTGCGCT
CATGCAAACCTGGGTGGATCGCATGGTCGCTCTGCGTGAGCGGGGCATTGAGGTGGTGATCGTCTCATCCGGGGCAGTGG
CCGAAGGCATGACCCGCCTGGGTTGGTCCGCTCGCCCGGATTCAGTGCATGAGTTGCAGGCTGCCGCTGCGGTAGGGCAG
ATGGGGCTGGTGCAGGCCTGGGAGACCCGTTTTCAGCAGCACCAGCTGCATACCGCCCAGGTGCTACTTACCCATGATGA
CCTTTCTGACCGTAAGCGCTATCTCAATGCGCGCTCCACGCTGCTAACCTTGCTGGGCTTTGGTGTGGTGCCGGTGGTAA
ACGAAAACGATACCGTGGTTACCGATGAAATTCGCTTTGGCGATAACGACACGTTGGCGGCGTTGGTGGCCAATCTTGTA
GAGGCTGATCGGCTGTTGATCCTGACGGATCAGGAGGGGTTGTTCGATGCAGACCCGCGTAGCAATGCTGATGCTAATCT
GATTCGCGAAGCGCGTGCCTCGGACCCGGCCATTGTCCGTGTGGCTGGCGGCGGCGGTTTGTTGGGGCGCGGAGGTATGG
CCACCAAGGTACGAGCTGGAAAGCTGGCGGCCCGTTCTGGTGCTATTACAACGATTGCGTCAGGGCGTAGTCCGGAGGTG
TTGATTGAGCTTGCTGACGGACAGGGGCCGGGGACTACGCTGTTGCCGGATACCACGCCAATGAATGCACGCAAGCAATG
GTTGGCAGGGCATTTGCAGATGCGTGGGCGGTTGGTGCTGGATGCTGGGGCGGTGGTGCGTTTGCGTGAAGGTGGCTCTA
GCCTGTTGCCGGTAGGAGTGGTGGATGTGTTTGGCCAGTTTTCCCGTGGCGAAATGGTGGCCTGTGAAGATGAGCGTGGC
GAGCGGGTTGGCTGTGGCCTGGTGAATTATGATGCGGCAGATGCGCGCCGGATTTGTCGTAAGAAAAGTGCTGAAATCGC
CGGTGTGCTGGGTTTCATGAACGAGGAAGAGCTGATCCACCGGGATAACCTGGTGTTGATGGAGGCCGGGAGCCGGGTTT
CGGGCGCTTGA

Upstream 100 bases:

>100_bases
GATCATGATGTGGAGGTTGTCTACGAGCCCTAGTGGACTTGTTCTGGTGCGCGTGAATGAATCAATGTCGGATGTTTGTC
TCAGGTAATAAGGAGTGAGC

Downstream 100 bases:

>100_bases
TGTGGGGGCTAGCTGGAATCTTTGCCGTCTGGTGCCCTTAAGTGTCAGACATAAAAAAACCGGCTTAGGGCCGGTTTTTT
TATGTACACCAAGAGGCTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MADRQRWVIKIGSALLTNDGKGLDCALMQTWVDRMVALRERGIEVVIVSSGAVAEGMTRLGWSARPDSVHELQAAAAVGQ
MGLVQAWETRFQQHQLHTAQVLLTHDDLSDRKRYLNARSTLLTLLGFGVVPVVNENDTVVTDEIRFGDNDTLAALVANLV
EADRLLILTDQEGLFDADPRSNADANLIREARASDPAIVRVAGGGGLLGRGGMATKVRAGKLAARSGAITTIASGRSPEV
LIELADGQGPGTTLLPDTTPMNARKQWLAGHLQMRGRLVLDAGAVVRLREGGSSLLPVGVVDVFGQFSRGEMVACEDERG
ERVGCGLVNYDAADARRICRKKSAEIAGVLGFMNEEELIHRDNLVLMEAGSRVSGA

Sequences:

>Translated_376_residues
MADRQRWVIKIGSALLTNDGKGLDCALMQTWVDRMVALRERGIEVVIVSSGAVAEGMTRLGWSARPDSVHELQAAAAVGQ
MGLVQAWETRFQQHQLHTAQVLLTHDDLSDRKRYLNARSTLLTLLGFGVVPVVNENDTVVTDEIRFGDNDTLAALVANLV
EADRLLILTDQEGLFDADPRSNADANLIREARASDPAIVRVAGGGGLLGRGGMATKVRAGKLAARSGAITTIASGRSPEV
LIELADGQGPGTTLLPDTTPMNARKQWLAGHLQMRGRLVLDAGAVVRLREGGSSLLPVGVVDVFGQFSRGEMVACEDERG
ERVGCGLVNYDAADARRICRKKSAEIAGVLGFMNEEELIHRDNLVLMEAGSRVSGA
>Mature_375_residues
ADRQRWVIKIGSALLTNDGKGLDCALMQTWVDRMVALRERGIEVVIVSSGAVAEGMTRLGWSARPDSVHELQAAAAVGQM
GLVQAWETRFQQHQLHTAQVLLTHDDLSDRKRYLNARSTLLTLLGFGVVPVVNENDTVVTDEIRFGDNDTLAALVANLVE
ADRLLILTDQEGLFDADPRSNADANLIREARASDPAIVRVAGGGGLLGRGGMATKVRAGKLAARSGAITTIASGRSPEVL
IELADGQGPGTTLLPDTTPMNARKQWLAGHLQMRGRLVLDAGAVVRLREGGSSLLPVGVVDVFGQFSRGEMVACEDERGE
RVGCGLVNYDAADARRICRKKSAEIAGVLGFMNEEELIHRDNLVLMEAGSRVSGA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=282, Percent_Identity=35.1063829787234, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI21361368, Length=284, Percent_Identity=34.8591549295775, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.0765027322404, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17569967, Length=266, Percent_Identity=32.7067669172932, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=266, Percent_Identity=32.7067669172932, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=33.4140435835351, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=422, Percent_Identity=33.175355450237, Blast_Score=192, Evalue=5e-50,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=30.2405498281787, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ALCBS (Q0VSE5)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692175.1
- ProteinModelPortal:   Q0VSE5
- SMR:   Q0VSE5
- STRING:   Q0VSE5
- GeneID:   4211220
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0455
- NMPDR:   fig|393595.12.peg.454
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q0VSE5
- ProtClustDB:   PRK05429
- BioCyc:   ABOR393595:ABO_0455-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40278; Mature: 40146

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRQRWVIKIGSALLTNDGKGLDCALMQTWVDRMVALRERGIEVVIVSSGAVAEGMTRL
CCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHC
GWSARPDSVHELQAAAAVGQMGLVQAWETRFQQHQLHTAQVLLTHDDLSDRKRYLNARST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHH
LLTLLGFGVVPVVNENDTVVTDEIRFGDNDTLAALVANLVEADRLLILTDQEGLFDADPR
HHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
SNADANLIREARASDPAIVRVAGGGGLLGRGGMATKVRAGKLAARSGAITTIASGRSPEV
CCCCHHHHHHHCCCCCCEEEEECCCCEECCCCCCEEEECCCCCCCCCCEEEEECCCCCEE
LIELADGQGPGTTLLPDTTPMNARKQWLAGHLQMRGRLVLDAGAVVRLREGGSSLLPVGV
EEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCHHH
VDVFGQFSRGEMVACEDERGERVGCGLVNYDAADARRICRKKSAEIAGVLGFMNEEELIH
HHHHCCCCCCCEEEECCCCCCEEECEEECCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHH
RDNLVLMEAGSRVSGA
CCCEEEEECCCCCCCC
>Mature Secondary Structure 
ADRQRWVIKIGSALLTNDGKGLDCALMQTWVDRMVALRERGIEVVIVSSGAVAEGMTRL
CCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHC
GWSARPDSVHELQAAAAVGQMGLVQAWETRFQQHQLHTAQVLLTHDDLSDRKRYLNARST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHH
LLTLLGFGVVPVVNENDTVVTDEIRFGDNDTLAALVANLVEADRLLILTDQEGLFDADPR
HHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
SNADANLIREARASDPAIVRVAGGGGLLGRGGMATKVRAGKLAARSGAITTIASGRSPEV
CCCCHHHHHHHCCCCCCEEEEECCCCEECCCCCCEEEECCCCCCCCCCEEEEECCCCCEE
LIELADGQGPGTTLLPDTTPMNARKQWLAGHLQMRGRLVLDAGAVVRLREGGSSLLPVGV
EEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCHHH
VDVFGQFSRGEMVACEDERGERVGCGLVNYDAADARRICRKKSAEIAGVLGFMNEEELIH
HHHHCCCCCCCEEEECCCCCCEEECEEECCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHH
RDNLVLMEAGSRVSGA
CCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA