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The map label for this gene is trpA

Identifier: 110681034

GI number: 110681034

Start: 3763301

End: 3764092

Strand: Direct

Name: trpA

Synonym: RD1_3895

Alternate gene names: 110681034

Gene position: 3763301-3764092 (Clockwise)

Preceding gene: 110681031

Following gene: 110681035

Centisome position: 91.05

GC content: 60.35

Gene sequence:

>792_bases
ATGACCCGTATCGACGACAAATTCGCCCAGCTTGCCAAGGACGGCAAGAAAGCCTTCGTGGCCTATGTGATGGCGGGTGA
TCCGAACTATGAAACCTCGCTGGAAGTCGTCAAAGGGTTGCCTGCGGCGGGGGTGGATGTGATCGAACTGGGCCTGCCTT
TTACGGACCCGATGGCGGACGGGCCGACCATCCAACTGGCCGGTCAACGCGCACTGGAGGCGGGCATGACGCTGCAAAAG
ACGCTGGACCTCGCCCGCGCCTTTCGCAAGGACGACCAGACAACCCCCATCGTGATGATGGGCTATTACAATCCGATCTA
CAGCCGCGGCGTTGATCGTTTTCTGGCCGAGGCCAAGGACGCGGGCATTGATGGATTGATCGTTGTGGATTTGCCACCGG
AGGAAGACAGCGAATTGTGTCTGCCCGCGCAAGCCGCCGGTCTGAACTTCATCCGATTGGCAACGCCCACCACCGATGAC
AAGCGCCTGCCGCGCGTTTTGCAAAACACCTCCGGTTTTGTCTATTATGTCTCGATCACGGGCATCACCGGTGCGGCCGA
AGCGCAAGCGGTCGATGTCGGCCCCGAAGTGGCGCGTATCAAATCCGCCGCCGCCCTGCCCGTAATCGTCGGTTTCGGCG
TGAACACCCCTGAAAAGTCCAAAGCCATCGCAGAGGTGGCCGACGGCGTGGTCGTGGGGTCCGCCATTGTCGGAAAGATC
GGCGCAGGCCAAAGCGTTTCGGATGTTCTGGCCTTTGTCAAAACACTCGCAGACGGCGCCCACAGCGTGTGA

Upstream 100 bases:

>100_bases
AACCACCCGCTGAGTGGGCGCGAAGCGCTTGGCACCTTTGATGCATTGATTTCCCCGGCGGTTTTGGGCATGTGAAGCTC
AAATGCTGAAAGGTGCCGCC

Downstream 100 bases:

>100_bases
TTGAAACATAGAGAGCCCGCGATTTGCTTTGCCGCCAGCGAGGCCATCGAACATGGGCACTGCCTTGCAAATCCGCTCCG
AACGCATTTCCTTTTGCACT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTRIDDKFAQLAKDGKKAFVAYVMAGDPNYETSLEVVKGLPAAGVDVIELGLPFTDPMADGPTIQLAGQRALEAGMTLQK
TLDLARAFRKDDQTTPIVMMGYYNPIYSRGVDRFLAEAKDAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDD
KRLPRVLQNTSGFVYYVSITGITGAAEAQAVDVGPEVARIKSAAALPVIVGFGVNTPEKSKAIAEVADGVVVGSAIVGKI
GAGQSVSDVLAFVKTLADGAHSV

Sequences:

>Translated_263_residues
MTRIDDKFAQLAKDGKKAFVAYVMAGDPNYETSLEVVKGLPAAGVDVIELGLPFTDPMADGPTIQLAGQRALEAGMTLQK
TLDLARAFRKDDQTTPIVMMGYYNPIYSRGVDRFLAEAKDAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDD
KRLPRVLQNTSGFVYYVSITGITGAAEAQAVDVGPEVARIKSAAALPVIVGFGVNTPEKSKAIAEVADGVVVGSAIVGKI
GAGQSVSDVLAFVKTLADGAHSV
>Mature_262_residues
TRIDDKFAQLAKDGKKAFVAYVMAGDPNYETSLEVVKGLPAAGVDVIELGLPFTDPMADGPTIQLAGQRALEAGMTLQKT
LDLARAFRKDDQTTPIVMMGYYNPIYSRGVDRFLAEAKDAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDDK
RLPRVLQNTSGFVYYVSITGITGAAEAQAVDVGPEVARIKSAAALPVIVGFGVNTPEKSKAIAEVADGVVVGSAIVGKIG
AGQSVSDVLAFVKTLADGAHSV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=221, Percent_Identity=36.6515837104072, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=266, Percent_Identity=40.6015037593985, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ROSDO (Q161I8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_684041.1
- ProteinModelPortal:   Q161I8
- SMR:   Q161I8
- STRING:   Q161I8
- GeneID:   4198782
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3895
- NMPDR:   fig|375451.6.peg.3620
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q161I8
- ProtClustDB:   PRK13111
- BioCyc:   RDEN375451:RD1_3895-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27539; Mature: 27408

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIDDKFAQLAKDGKKAFVAYVMAGDPNYETSLEVVKGLPAAGVDVIELGLPFTDPMAD
CCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPTIQLAGQRALEAGMTLQKTLDLARAFRKDDQTTPIVMMGYYNPIYSRGVDRFLAEAKD
CCEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
AGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDDKRLPRVLQNTSGFVYYVSIT
CCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEE
GITGAAEAQAVDVGPEVARIKSAAALPVIVGFGVNTPEKSKAIAEVADGVVVGSAIVGKI
CCCCCCCCEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEECHHHHHCC
GAGQSVSDVLAFVKTLADGAHSV
CCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TRIDDKFAQLAKDGKKAFVAYVMAGDPNYETSLEVVKGLPAAGVDVIELGLPFTDPMAD
CCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPTIQLAGQRALEAGMTLQKTLDLARAFRKDDQTTPIVMMGYYNPIYSRGVDRFLAEAKD
CCEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
AGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDDKRLPRVLQNTSGFVYYVSIT
CCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEE
GITGAAEAQAVDVGPEVARIKSAAALPVIVGFGVNTPEKSKAIAEVADGVVVGSAIVGKI
CCCCCCCCEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEECHHHHHCC
GAGQSVSDVLAFVKTLADGAHSV
CCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA