BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

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The map label for this gene is braF [H]

Identifier: 110680745

GI number: 110680745

Start: 3449735

End: 3450463

Strand: Direct

Name: braF [H]

Synonym: RD1_3587

Alternate gene names: 110680745

Gene position: 3449735-3450463 (Clockwise)

Preceding gene: 110680743

Following gene: 110680746

Centisome position: 83.47

GC content: 58.57

Gene sequence:

>729_bases
TTGTCACTGGAACTAAGGTCCATCAACGTTGCATTTGGCGGCTTCAAAGCCATCGATGATGTGACGATTTCCCTCAAGAA
GGCAGAAATCGCAGGGTTGATCGGCCCAAACGGTGCCGGCAAAACCACATGTGTGAACGTGATCACCGGGTTTCAGGACC
CCAGTTCCGGTACGGTCACGCTGGATGGTGAAAGCATCACCGGGAAAGCACCGCATGACATCCGACGCCGGGGTCTTGCG
CGCACCTTTCAGGCAGGCCGCCTGTTCACGGGACTTGATGTGCTCGACAACCTCGCGGTGACGGGCGTTGGTCTTGGGCA
TTCGCGCCGCGCGTCCGAAGATGAGGCGATGCGTCTGCTTGACTGGATGGGCGTTGCGCATCTGGCCGAACTTCCCGCTG
CCGGCCTGCCCTATACTGACGAGCGCCGCGTGGGCATTGCGCGTGCACTGATGTTTGCACCGGATTTTCTACTTCTCGAT
GAACCGGCTGCAGGCATGTCCGAAAAGGAAGCGGACGATCTCGCCCGGATCATCAGACGCATCGCAGACGAGCTTGAATG
CGGCGTTTTGCTGATTGAGCACAATGTCGGCCTCGTGCTGTCACTGTCCAATCACGTCTATGTTCTGGATGCGGGCAAGA
TTATCGAAGAGGGCGACAAGGCGGCAATTCTGGGCAGTCAGGCCGTGCGCGAAGCCTATCTGGGCGCTGACCAGATGGAG
GACGCGTAA

Upstream 100 bases:

>100_bases
AGATCGGCTTGCGGCTGTCCGTTCAGGCTGGCCGCAAGCCACGTAGTTGCATCTGCGCGCCGCAGCATCGGCGACGCACC
AACCACGAAGGATAGAATTG

Downstream 100 bases:

>100_bases
TGTCTAAGCTGGGCATATTCGATGTCGCTGTCAGATATGGCAAAGTGCCGGCCCTGTCAGACGTCTCCTTCACCGTCGAT
GAAGGTGAGCGGCTTTTCAT

Product: branched-chain amino acid ABC transporter, ATP-binding protein, putative

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSLELRSINVAFGGFKAIDDVTISLKKAEIAGLIGPNGAGKTTCVNVITGFQDPSSGTVTLDGESITGKAPHDIRRRGLA
RTFQAGRLFTGLDVLDNLAVTGVGLGHSRRASEDEAMRLLDWMGVAHLAELPAAGLPYTDERRVGIARALMFAPDFLLLD
EPAAGMSEKEADDLARIIRRIADELECGVLLIEHNVGLVLSLSNHVYVLDAGKIIEEGDKAAILGSQAVREAYLGADQME
DA

Sequences:

>Translated_242_residues
MSLELRSINVAFGGFKAIDDVTISLKKAEIAGLIGPNGAGKTTCVNVITGFQDPSSGTVTLDGESITGKAPHDIRRRGLA
RTFQAGRLFTGLDVLDNLAVTGVGLGHSRRASEDEAMRLLDWMGVAHLAELPAAGLPYTDERRVGIARALMFAPDFLLLD
EPAAGMSEKEADDLARIIRRIADELECGVLLIEHNVGLVLSLSNHVYVLDAGKIIEEGDKAAILGSQAVREAYLGADQME
DA
>Mature_241_residues
SLELRSINVAFGGFKAIDDVTISLKKAEIAGLIGPNGAGKTTCVNVITGFQDPSSGTVTLDGESITGKAPHDIRRRGLAR
TFQAGRLFTGLDVLDNLAVTGVGLGHSRRASEDEAMRLLDWMGVAHLAELPAAGLPYTDERRVGIARALMFAPDFLLLDE
PAAGMSEKEADDLARIIRRIADELECGVLLIEHNVGLVLSLSNHVYVLDAGKIIEEGDKAAILGSQAVREAYLGADQMED
A

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=205, Percent_Identity=27.8048780487805, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI27477115, Length=198, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI6005701, Length=198, Percent_Identity=28.7878787878788, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI171184400, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27262624, Length=194, Percent_Identity=25.2577319587629, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27262626, Length=194, Percent_Identity=25.2577319587629, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27436953, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI105990541, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI27881501, Length=200, Percent_Identity=28, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI30795238, Length=200, Percent_Identity=28, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI9955968, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI156105685, Length=236, Percent_Identity=27.1186440677966, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI9955966, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45243524, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI116734710, Length=170, Percent_Identity=30.5882352941176, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789864, Length=253, Percent_Identity=38.7351778656126, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1789593, Length=237, Percent_Identity=35.0210970464135, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI87081791, Length=232, Percent_Identity=27.5862068965517, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788761, Length=223, Percent_Identity=29.5964125560538, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787712, Length=213, Percent_Identity=28.6384976525822, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787089, Length=234, Percent_Identity=31.1965811965812, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081709, Length=232, Percent_Identity=27.1551724137931, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082268, Length=244, Percent_Identity=30.327868852459, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786872, Length=225, Percent_Identity=28, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788472, Length=218, Percent_Identity=27.5229357798165, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1788450, Length=235, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788165, Length=208, Percent_Identity=29.8076923076923, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787029, Length=234, Percent_Identity=24.7863247863248, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI48995001, Length=208, Percent_Identity=29.3269230769231, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789991, Length=228, Percent_Identity=30.7017543859649, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87081782, Length=167, Percent_Identity=33.5329341317365, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789672, Length=222, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786563, Length=210, Percent_Identity=29.0476190476191, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786253, Length=216, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1789586, Length=231, Percent_Identity=28.1385281385281, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=26.2931034482759, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787370, Length=225, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788210, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790467, Length=240, Percent_Identity=23.3333333333333, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790190, Length=225, Percent_Identity=25.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789032, Length=236, Percent_Identity=25.4237288135593, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789873, Length=209, Percent_Identity=29.6650717703349, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788644, Length=230, Percent_Identity=27.3913043478261, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786398, Length=209, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI48994943, Length=249, Percent_Identity=25.7028112449799, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786698, Length=202, Percent_Identity=27.2277227722772, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790525, Length=232, Percent_Identity=26.2931034482759, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787792, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786703, Length=182, Percent_Identity=29.1208791208791, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081834, Length=204, Percent_Identity=25, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788506, Length=226, Percent_Identity=26.9911504424779, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786803, Length=234, Percent_Identity=23.9316239316239, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=26.2672811059908, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787759, Length=243, Percent_Identity=27.5720164609054, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786319, Length=233, Percent_Identity=24.8927038626609, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790162, Length=243, Percent_Identity=23.0452674897119, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790544, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787758, Length=244, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082267, Length=214, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787547, Length=241, Percent_Identity=23.6514522821577, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788540, Length=223, Percent_Identity=27.3542600896861, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789962, Length=232, Percent_Identity=23.2758620689655, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994997, Length=237, Percent_Identity=24.8945147679325, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789963, Length=259, Percent_Identity=26.6409266409266, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=200, Percent_Identity=25, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787164, Length=213, Percent_Identity=27.2300469483568, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786345, Length=236, Percent_Identity=22.4576271186441, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790739, Length=239, Percent_Identity=25.9414225941423, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787112, Length=242, Percent_Identity=28.5123966942149, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367384, Length=243, Percent_Identity=26.7489711934156, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786981, Length=213, Percent_Identity=30.0469483568075, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI48994883, Length=236, Percent_Identity=23.728813559322, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17510237, Length=222, Percent_Identity=26.5765765765766, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983510, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17567265, Length=234, Percent_Identity=26.0683760683761, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=198, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=230, Percent_Identity=25.6521739130435, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=218, Percent_Identity=26.1467889908257, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=168, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=217, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24659289, Length=244, Percent_Identity=25, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666092, Length=228, Percent_Identity=25.4385964912281, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648314, Length=180, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643674, Length=224, Percent_Identity=27.6785714285714, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221512771, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641565, Length=245, Percent_Identity=24.8979591836735, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24581191, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581189, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581193, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581195, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363262, Length=220, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653245, Length=220, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574744, Length=223, Percent_Identity=25.1121076233184, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25751; Mature: 25620

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLELRSINVAFGGFKAIDDVTISLKKAEIAGLIGPNGAGKTTCVNVITGFQDPSSGTVT
CCEEEEEEEEEECCCEECCCEEEEEEEEEEEEEECCCCCCCHHEEEEEECCCCCCCCEEE
LDGESITGKAPHDIRRRGLARTFQAGRLFTGLDVLDNLAVTGVGLGHSRRASEDEAMRLL
ECCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCHHHEEECCCCCCCCCHHHHHHHH
DWMGVAHLAELPAAGLPYTDERRVGIARALMFAPDFLLLDEPAAGMSEKEADDLARIIRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
IADELECGVLLIEHNVGLVLSLSNHVYVLDAGKIIEEGDKAAILGSQAVREAYLGADQME
HHHHHCCCEEEEECCCEEEEEECCCEEEEECCHHHHCCCCEEEECHHHHHHHHCCCHHCC
DA
CC
>Mature Secondary Structure 
SLELRSINVAFGGFKAIDDVTISLKKAEIAGLIGPNGAGKTTCVNVITGFQDPSSGTVT
CEEEEEEEEEECCCEECCCEEEEEEEEEEEEEECCCCCCCHHEEEEEECCCCCCCCEEE
LDGESITGKAPHDIRRRGLARTFQAGRLFTGLDVLDNLAVTGVGLGHSRRASEDEAMRLL
ECCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCHHHEEECCCCCCCCCHHHHHHHH
DWMGVAHLAELPAAGLPYTDERRVGIARALMFAPDFLLLDEPAAGMSEKEADDLARIIRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
IADELECGVLLIEHNVGLVLSLSNHVYVLDAGKIIEEGDKAAILGSQAVREAYLGADQME
HHHHHCCCEEEEECCCEEEEEECCCEEEEECCHHHHCCCCEEEECHHHHHHHHCCCHHCC
DA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]