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The map label for this gene is gloB

Identifier: 110680699

GI number: 110680699

Start: 3400401

End: 3401168

Strand: Direct

Name: gloB

Synonym: RD1_3539

Alternate gene names: 110680699

Gene position: 3400401-3401168 (Clockwise)

Preceding gene: 110680689

Following gene: 110680700

Centisome position: 82.27

GC content: 60.42

Gene sequence:

>768_bases
ATGCCCATCGAGATACTGACCATTCCCTGCCTGAACGACAACTACGCGTTTCTGGTGCATGACACGGCGACGGGTCAGAC
CGCGCTGATTGATGTTCCCGAGGCGGCCCCGATTCTAAAGGTGCTTGCAGAACGGACGTGGCAGCTGAGTGAGGTCTGGA
TCACCCATCATCACGCAGACCACGTACAGGGCCTCGGAGATATCTTGTCCGCGCATCCGGCGCAGGTGCGCGGGGCAGCG
CGCGATGCGCACCGTCTGCCGGCACTTGATGTCGAATTGCGCGACGGGGAGAGCTTTGATTTCGCCGGCCACAAGGTCGA
CGTGATGGACGTTTCCGGACACACGGTCGGCCACATCGCCTATTATTGTGCAGACGCAAAGGCCGTGTTCACCGCAGATA
GCCTGATGGCACTGGGCTGCGGGCGCCTGTTCGAAGGCACCGCCGATCAGATGTGGGACAGCCTGAGCAAGCTCGCGGCC
CTGCCCTCCGAGACACAAGTATATTCGGGTCATGAATACACCGCCGCCAACGCTAGGTTTGCATTAACCATTGATCCGCA
TAATCCAGATCTTATATCGCGCTCGGAGGGCATAACGGCGGCGCGCGCGGCCGCAAAGCCCACCGTTCCATCCACCTTGG
CGACAGAAGCCGCAACAAACCCCTTTCTGCGGGCCGCCGACCCTGCCATCCGCGCGCACCTCAATATGCAGCACGCGAGC
GACAGCGAAGTCTTTGCTGAAATTCGCGCCCGCAAAGACGCATTCTAA

Upstream 100 bases:

>100_bases
AGTAGAAATTTCGCAGGTCCTGTACGTCCAGATGCATTGCGGCCAGCCTCGTGATCGGTCACTCTTGAAACCAGTGTCGC
AAAGCCGGAGGAAAATGACC

Downstream 100 bases:

>100_bases
CGCCCACATCGTGACCCCTGCGCGGCCTGAATCACAAAATGTGAATGGAAAAATGAAGAAAAAACTTGAAGCCGCGCTAG
GATCAACCAAACTCTAACTT

Product: hydroxyacylglutathione hydrolase, putative

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPIEILTIPCLNDNYAFLVHDTATGQTALIDVPEAAPILKVLAERTWQLSEVWITHHHADHVQGLGDILSAHPAQVRGAA
RDAHRLPALDVELRDGESFDFAGHKVDVMDVSGHTVGHIAYYCADAKAVFTADSLMALGCGRLFEGTADQMWDSLSKLAA
LPSETQVYSGHEYTAANARFALTIDPHNPDLISRSEGITAARAAAKPTVPSTLATEAATNPFLRAADPAIRAHLNMQHAS
DSEVFAEIRARKDAF

Sequences:

>Translated_255_residues
MPIEILTIPCLNDNYAFLVHDTATGQTALIDVPEAAPILKVLAERTWQLSEVWITHHHADHVQGLGDILSAHPAQVRGAA
RDAHRLPALDVELRDGESFDFAGHKVDVMDVSGHTVGHIAYYCADAKAVFTADSLMALGCGRLFEGTADQMWDSLSKLAA
LPSETQVYSGHEYTAANARFALTIDPHNPDLISRSEGITAARAAAKPTVPSTLATEAATNPFLRAADPAIRAHLNMQHAS
DSEVFAEIRARKDAF
>Mature_254_residues
PIEILTIPCLNDNYAFLVHDTATGQTALIDVPEAAPILKVLAERTWQLSEVWITHHHADHVQGLGDILSAHPAQVRGAAR
DAHRLPALDVELRDGESFDFAGHKVDVMDVSGHTVGHIAYYCADAKAVFTADSLMALGCGRLFEGTADQMWDSLSKLAAL
PSETQVYSGHEYTAANARFALTIDPHNPDLISRSEGITAARAAAKPTVPSTLATEAATNPFLRAADPAIRAHLNMQHASD
SEVFAEIRARKDAF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI14150041, Length=256, Percent_Identity=34.765625, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=32.9545454545455, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=32.9545454545455, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI46361987, Length=192, Percent_Identity=35.9375, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI116642887, Length=264, Percent_Identity=29.1666666666667, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI21703352, Length=264, Percent_Identity=29.1666666666667, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI41327741, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=37.0517928286853, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17536925, Length=224, Percent_Identity=35.7142857142857, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538952, Length=180, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=28.996282527881, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=266, Percent_Identity=27.4436090225564, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=36.9230769230769, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=36.9230769230769, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=36.9230769230769, Blast_Score=170, Evalue=7e-43,
Organism=Drosophila melanogaster, GI221330176, Length=182, Percent_Identity=29.6703296703297, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ROSDO (Q162S3)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683706.1
- ProteinModelPortal:   Q162S3
- SMR:   Q162S3
- STRING:   Q162S3
- GeneID:   4196229
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3539
- NMPDR:   fig|375451.6.peg.3291
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q162S3
- ProtClustDB:   CLSK836335
- BioCyc:   RDEN375451:RD1_3539-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27523; Mature: 27392

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00469 NDP_KINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILTIPCLNDNYAFLVHDTATGQTALIDVPEAAPILKVLAERTWQLSEVWITHHHAD
CCEEEEEEEEECCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCHH
HVQGLGDILSAHPAQVRGAARDAHRLPALDVELRDGESFDFAGHKVDVMDVSGHTVGHIA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
YYCADAKAVFTADSLMALGCGRLFEGTADQMWDSLSKLAALPSETQVYSGHEYTAANARF
EEECCCCEEEEHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCEE
ALTIDPHNPDLISRSEGITAARAAAKPTVPSTLATEAATNPFLRAADPAIRAHLNMQHAS
EEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEECCCCC
DSEVFAEIRARKDAF
CHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIEILTIPCLNDNYAFLVHDTATGQTALIDVPEAAPILKVLAERTWQLSEVWITHHHAD
CEEEEEEEEECCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCHH
HVQGLGDILSAHPAQVRGAARDAHRLPALDVELRDGESFDFAGHKVDVMDVSGHTVGHIA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
YYCADAKAVFTADSLMALGCGRLFEGTADQMWDSLSKLAALPSETQVYSGHEYTAANARF
EEECCCCEEEEHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCEE
ALTIDPHNPDLISRSEGITAARAAAKPTVPSTLATEAATNPFLRAADPAIRAHLNMQHAS
EEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEECCCCC
DSEVFAEIRARKDAF
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA