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The map label for this gene is insF1 [H]

Identifier: 110680621

GI number: 110680621

Start: 3319831

End: 3320646

Strand: Direct

Name: insF1 [H]

Synonym: RD1_3452

Alternate gene names: 110680621

Gene position: 3319831-3320646 (Clockwise)

Preceding gene: 110680615

Following gene: 110680622

Centisome position: 80.32

GC content: 57.84

Gene sequence:

>816_bases
ATGTTTGGGTTTGTGGCGAAGCACCGAGGGGTCTGGCCAGTGAGTTGGATGTGTGATGCGCTCGGTGTGTCACGAAGCGG
CTTTCATGCCTGGCTGACGCGACCGCGCAGCGACCGGTCTATTCGCGACGAAGAGCTTTCGGCGGCGATCCGTGCGAGCT
TTCTGCGATCGGACCGAACCTATGGAGCCCGACGGGTCTGGCACGATCTTCTCGAAGAAGGTTATGCCTGTGGCCTGCAT
TGCGTTGAACGATTGATGCGACAAGCAGCGTTCAAGGCCCGCCCCAAGCGTCGGCAACCGCCAAAAGACCAAGGCATGAG
GTCGGTCATAGCTGCCAACGTGCTTGAACGAGAGTTTGATGCGGATGGGCCGAACCAGAAATGGGTGGCCGACTTCACCT
ATGTCTGGACCGCGCAAGGGTGGCTCTACGTTGCAGCCGTCATCGATCTGTTCTCGCGGCGTGTCGTTGGCTGGTCAATG
AGCGAACAAATGACGTCTCAGATGGTCACGGACGCTCTGATCATGGCGATCTGGCGCAGAGGCAGGCCCGACGAGTTGCT
GCACCATTCCGACCAGGGCAGCCAATACACCAGTGAGCCGTTCCAGCGACTGATGGCCGATCACGGCATCACCTGTTCGA
TGAGCCGTTCAGGGAATGTCTGGGACAACGCTGCAATGGAAAGCTTCTTCTCTTCGCTTAAGACAGAACGGATCAAACGC
AAAACCTACAGAACCAGAAAGCACGCGCGCGCAGATGTCTTCGATTACATCGAACGCTTCTACAACCCGAAGCGTCGCCA
CTCGACCATCGGCTAG

Upstream 100 bases:

>100_bases
AGGAAGAGCTCAGGATTCTTCGACGTGAGGTCGCCAAGCTGAAGGCGGAGCGTGACATCTTAAAAAAAGCGGCCGCCTAC
TTCGCCAAGGACCAGCTGTG

Downstream 100 bases:

>100_bases
CTAAGCCCTATCACATTCGAGGAGCGAGCTATGAAAGCTTAAGTTGCCGTCCACGAAACCGGCAGCAGGCCAGTTTGATG
ACTACAGCTGCCATCGCCTC

Product: transposase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MFGFVAKHRGVWPVSWMCDALGVSRSGFHAWLTRPRSDRSIRDEELSAAIRASFLRSDRTYGARRVWHDLLEEGYACGLH
CVERLMRQAAFKARPKRRQPPKDQGMRSVIAANVLEREFDADGPNQKWVADFTYVWTAQGWLYVAAVIDLFSRRVVGWSM
SEQMTSQMVTDALIMAIWRRGRPDELLHHSDQGSQYTSEPFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERIKR
KTYRTRKHARADVFDYIERFYNPKRRHSTIG

Sequences:

>Translated_271_residues
MFGFVAKHRGVWPVSWMCDALGVSRSGFHAWLTRPRSDRSIRDEELSAAIRASFLRSDRTYGARRVWHDLLEEGYACGLH
CVERLMRQAAFKARPKRRQPPKDQGMRSVIAANVLEREFDADGPNQKWVADFTYVWTAQGWLYVAAVIDLFSRRVVGWSM
SEQMTSQMVTDALIMAIWRRGRPDELLHHSDQGSQYTSEPFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERIKR
KTYRTRKHARADVFDYIERFYNPKRRHSTIG
>Mature_271_residues
MFGFVAKHRGVWPVSWMCDALGVSRSGFHAWLTRPRSDRSIRDEELSAAIRASFLRSDRTYGARRVWHDLLEEGYACGLH
CVERLMRQAAFKARPKRRQPPKDQGMRSVIAANVLEREFDADGPNQKWVADFTYVWTAQGWLYVAAVIDLFSRRVVGWSM
SEQMTSQMVTDALIMAIWRRGRPDELLHHSDQGSQYTSEPFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERIKR
KTYRTRKHARADVFDYIERFYNPKRRHSTIG

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=246, Percent_Identity=34.9593495934959, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=41.970802919708, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=41.970802919708, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=41.970802919708, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=41.970802919708, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=41.970802919708, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789981, Length=262, Percent_Identity=34.3511450381679, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31519; Mature: 31519

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFVAKHRGVWPVSWMCDALGVSRSGFHAWLTRPRSDRSIRDEELSAAIRASFLRSDRT
CCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGARRVWHDLLEEGYACGLHCVERLMRQAAFKARPKRRQPPKDQGMRSVIAANVLEREFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ADGPNQKWVADFTYVWTAQGWLYVAAVIDLFSRRVVGWSMSEQMTSQMVTDALIMAIWRR
CCCCCCCEEEHHHHEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GRPDELLHHSDQGSQYTSEPFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERIKR
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KTYRTRKHARADVFDYIERFYNPKRRHSTIG
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MFGFVAKHRGVWPVSWMCDALGVSRSGFHAWLTRPRSDRSIRDEELSAAIRASFLRSDRT
CCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGARRVWHDLLEEGYACGLHCVERLMRQAAFKARPKRRQPPKDQGMRSVIAANVLEREFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ADGPNQKWVADFTYVWTAQGWLYVAAVIDLFSRRVVGWSMSEQMTSQMVTDALIMAIWRR
CCCCCCCEEEHHHHEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GRPDELLHHSDQGSQYTSEPFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERIKR
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KTYRTRKHARADVFDYIERFYNPKRRHSTIG
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]